Unknown bacteria determined to be Alcaligenes faecalis because of its morphological, physiological and metabolic properties. In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s
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Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by
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Enzyme Experiment * Isolation of yeast invertase * Effect of temperature and pH Objectives * Determine the activity of an enzyme (invertase) * Determine the effects of pH and temperature in each activity Parts of the Experiment 1. Isolation 2. Sucrose Assay – to generate or construct a standard curve 3. Effect of temperature and pH a. Determine and graph the optimum pH and Temperature Enzymes will act on a compound or biomolecule (substrate, e.g. sucrose) to
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| | | | Exploring the Effects of Enzymes on Fat Using Phenol RedVeronica ChengSBI 3U0Turner05 December 2014 | | Introduction Enzymes are proteins which help break down chemical substances such as other proteins, carbohydrates or fats that are necessary for life (Novozymes, 2014). In this experiment, the effects of water, pancreatic juice, and trypsin solutions were observed on milk. The use of the phenol red indicator helps detect the pH level. The lower pH level will cause the colour
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different types of necrosis that occur the first being cogulative necrosis, which occurs when the structure and enzymatic protein undergoes primary denaturation. Another important necrosis is colliquative necrosis caused by a highly powerful catalytic enzyme
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Molecules, Biodiversity, Food and Health Biological Molecules Biological Molecules (a) describe how hydrogen bonding occurs between water molecules, and relate this, and other properties of water, to the roles of water in living organisms; Water is a polar molecule. This is because the oxygen atom pulls the shared electrons towards it, meaning that water is slightly negatively charged at the oxygen and positively charged at the hydrogen ends, so they can form hydrogen bonds with each other
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A lysosome (derived from the Greek words lysis, meaning "dissolution/ destruction", and soma, "body") is a membrane-bound cell organellefound in most animal cells. They are known to contain more than 50 different enzymes, which are all optimally active at an acidic environment of about pH 4.5 For this function they are popularly referred to as "suicide bags" or "suicide sacs" of the cell. hey were discovered and named by Belgian biologist Christian de Duve, who eventually received the Nobel Prize
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Effect of plasma on flour hydration properties of parboiled rice flour: Flour hydrations properties of plasma treated parboiled rice flour are shown in the table 1.There is no significant difference (p>0.05) in hydration properties between the samples. But it was found that slight increase in WHC at higher Power and time from 1.79(g/g) to 1.88(g/g). Water binding capacity of flour samples was found to be increased after treatment. Significant increase (P<0.05) in WBC was found both untreated and
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B) For Purification: Thitima Sarachat et al (2009) worked on Pseudomonas aeruginosa SP4 and cultivated to produce a rhamnolipid biosurfactant from a nutrient broth with palm oil. The foam fractionation technique in batch mode was used for the recovery of the excreted biosurfactant from the free-cell culture medium. The effects of air flow rate, initial foam height, the pore size of the sintered glass disk, initial liquid volume, and operation time on the process performance were studied. The results
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Enzymes are globular proteins folded into a complex 3-dimensional shape that contain a special surface region called the active site where specific substrate can bind structurally and chemically. They act as catalysts, meaning that they are substances which lower the activation energy required for a chemical reaction to occur and therefore increases the rate of the reaction. Activation Energy is the minimum energy barrier needed to be overcome before a reaction can occur by providing an alternative
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