the news article? 2. Why is boosting crop yields necessary? 3. What enzyme did the researchers target and what does the enzyme do? 4. Why do plants have so much of this enzyme? 5. The Cornell researchers took this enzyme from another source to put into what crop plant? What source did they take the enzyme from? 6. In what way is the substitute enzyme more wasteful than the original enzyme? 7. What is it called when Rubisco uses O2 instead of CO2, and why is
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the process of cutting DNA molecules into smaller pieces with special enzymes such as, BamH1 and EcoR1. One had to determine which of the two plasmids, A or B, were pGLO or pWEB. A plasmid is a small circular DNA strand in the cytoplasm of a bacterium (Isite, 2013). In order to determine that, one had to use BamH1 and EcoR1 on two tubes each one with plasmid A and the other with plasmid B to observe the cuts made by each enzyme. The hypothesis for this segment of the laboratory exercise states that
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important? The chemical problem explored in this research is based upon anticancer agents and enzyme inhibitors. The scientists looked for caging groups other than the known Ru(bpy)2 that would bond biologically active molecules to either organic or metal-based protecting groups cleaved with light. These species would be used in medical situations to treat neurotransmitter defects, cancers, and enzyme dysfunctions. 2. What information is needed to solve the problem? One piece of extremely important
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insight commentary Virtual screening of chemical libraries Brian K. Shoichet Department of Pharmaceutical Chemistry, University of California, 600 16th Street, San Francisco, California 94143-2240, USA (e-mail: shoichet@cgl.ucsf.edu) Virtual screening uses computer-based methods to discover new ligands on the basis of biological structures. Although widely heralded in the 1970s and 1980s, the technique has since struggled to meet its initial promise, and drug discovery remains dominated by
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the cell walls to expose the DNA and the use of enzymes to remove contaminants. The DNA is analyzed for purity by taking the absorbance. The pure DNA is then visualized by gel electrophoresis. The DNA extraction of plant seeds is difficult because of their cell wall. The method used to break the cell wall includes grinding the seeds with liquid nitrogen. The addition of DNAzol is used to isolate genomic DNA (Chomczynski et al. 1997). Restriction enzymes are necessary to fragment patterns of the DNA
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Molecular Systems Biology 4; Article number 228; doi:10.1038/msb.2008.60 Citation: Molecular Systems Biology 4:228 & 2008 EMBO and Macmillan Publishers Limited All rights reserved 1744-4292/08 www.molecularsystemsbiology.com Finding multiple target optimal intervention in diseaserelated molecular network Kun Yang1,2, Hongjun Bai1,2, Qi Ouyang2, Luhua Lai1,2,* and Chao Tang2,3 1 Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable
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This article was downloaded by: [Universite de Lorraine] On: 10 February 2012, At: 06:13 Publisher: Taylor & Francis Informa Ltd Registered in England and Wales Registered Number: 1072954 Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH, UK Journal of Environmental Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering Publication details, including instructions for authors and subscription information: http://www.tandfonline.com/loi/lesa20
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Discussion The results of the experiment did not support the created hypothesis that the poisonous enzyme PTU (Phenylthiourea) was a competitive inhibitor. In the experiment to test whether PTU was competitive a catechol oxidase substrate was added to the solution to determine it’s inhibition. As more of the substrate was added, no reaction occurred and the substance remained consistently clear. However, the results indicated that the catechol that was used continued to be inhibited
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Peroxidases are enzymes that use hydrogen peroxide as an electron acceptor to catalyze oxidative reactions. Peroxidase is believed to play a major role in synthesizing cell walls, preventing toxins and responding to stress. It is responsible for the purple color that is seen throughout the experiments. Peroxidase can be found in different locations in a plant in the form of isoenzymes. Many different experiments can be done to locate, visualize and quantify the activity of peroxidase enzymes. These include
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adenosine tri-phosphate (ATP). The process follows oxidation (catabolic) and reduction (anabolic) pathways. Processes involved are glycolysis, Krebs or tricarboxylic acid (TCA) cycle, and the electron transport chain. One step in the TCA cycle is the enzyme-catalyzed conversion of succinate to fumarate in a redox reaction. In intact cells succinate loses hydrogen ions and electrons to FAD to form fumarate. This step in the TCA cycle will be used to study the rate of cellular respiration under different
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