Name of Institution Abstract Polymerase chain reaction (PCR) is greatly used in molecular genetics. It entails amplification of a single DNA strand into millions of similar DNA fragments. It involves three stages in each cycle. It is repeated to about 30 cycles. This method is vital as it is used in various processes such molecular identification, genetic engineering, and sequencing. The three stages in each
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electrophoresis Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.[1] Nucleic acid molecules are separated
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Biology: Concepts and Connections, 6e (Campbell) Chapter 12 DNA Technology and Genomics Multiple-Choice Questions 1) When DNA fingerprinting was first used, A) genetic evidence was collected using only DNA from blood. B) blood samples from theGenomic libraries can be constructed using either bacterial plasmids or what other vector? crime scene were used to match the blood of a person who confessed. C) the two semen samples did not match the person who initially
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the phosphate backbone of the DNA. The active site of the endonuclease perform this cleavage by binding to the side chain of certain amino acids to the phosphate group through a chemical bond. This dissolves the preexisting bond between the deoxyribose sugar and the phosphate resulting in a breakage with in the DNA chain at a specific location. (3, 7) One characteristic feature of restriction endonucleases is that they cut at a very particular site having a specific DNA sequence. This specific sequence
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Wednesday, July 24, 2013 Total community DNA • Extract DNA from soil – – – – remove cells from soil separate cells from soil lyse cells separate DNA from cells – purify DNA • Extract DNA from soil – Extract DNA from cells in presence of soil • Bead-beating • chemical or enzymatic treatment – Sodium dodecyl sulfate or lysozyme Wednesday, July 24, 2013 DNA purification • Cesium chloride gradient centrifugation • Kits Low density DNA High density Wednesday, July 24, 2013
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Genetic Testing Overview Tracye D Burgess BIO 100 Dr. F. Zamamiri-Davis December 9, 2013 Genetic Testing Overview Tracye D Burgess BIO 100 Dr. F. Zamamiri-Davis December 9, 2013 Outline I. Genetic Testing of Diseases a. Genetic Testing II. Types of Genetic Testing a. Three Common Types b. Parental Testing c. Conclusion Impact Statement Genetic testing is a complex process, and the results depend both
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2003. Cloning is the process of replicating the genes present within a DNA molecule, in order to be able to make copies of an organism. I remember the first cloning of a mammal; it was a sheep by the name of “Dolly,” whom was successfully cloned in 1997, by a group of Scottish scientists. Cloning could make it achievable for us to get modified organisms. But we must also look at how will cloning fit into our ethical values? DNA fingerprints are often used as evidence in criminal law cases and with
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Creative Technology Exam 2 Study Guide 1) Uses a promoter- transcription 2) An anticodon is involved in this process - translation 3) Codons are involved - translation 4) Uses DNA Polymerase- replication 5) Polymerase chain reaction is a “synthetic” version of this 6) RNA polymerase is used - transcription 7) Ribosomes are used- translation 8) tRNA is used - translation 9) mRNA is produced - transcription 10) mRNA is read - translation 11) Important
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LATEST BIOCHEMICAL TECHNIQUES CAPILLARY ELECTROPHORESIS Capillary electrophoresis is an analytical technique that separates ions based on their electrophoretic mobility with the use of an applied voltage. The electrophoretic mobility is dependent upon the charge of the molecule, the viscosity, and the atom’s radius. In conventional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. The rate at which the particles moves is
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single DNA molecule even though it is initially present in a mixture containing many different DNA molecules. It is used to amplify a specific DNA (target) sequence lying between known positions (flanks) on a double-stranded (ds) DNA molecule. The polymerase chain reaction can be used to amplify both double and single stranded DNA. In order to perform PCR, one must know at least a portion of the sequence of the target DNA molecule that has to be copied. Generally, PCR amplifies small DNA targets
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