Suppression is a technique that plays an integral role in ion exchange chromatography as well as conductivity detection. It is commonly used in the detection and analysis of molecules such as ions as well as organic acids. There are numerous factors behind its popularity and effectiveness. These factors includes its ability to decrease background conductivity of the eluent which aids in the accuracy of result, minimizing of baseline noises which enables smoother analyses, optimization of signal to
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proper growth, development and maintenance of bone, connective tissue, brain, heart and other body organs. Copper is involved in absorption of iron, metabolism of cholesterol and glucose. Copper has a role in synthesis and release of proteins and enzymes involved in cellular energy, nerve regulation, blood clotting and oxygen transport. Copper stimulates immune system, repairing injured tissues and in healing. Copper helps to neutralize free radicals which cause damage to cells. The human body has
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of proteins is a three-dimensional structure formed by the folding up of a whole polypeptide chain. Every protein has unique tertiary structure, which is responsible for its properties and function. For example, the shape of the active site in an enzyme is due to its treaty structure. Quaternary structure of proteins found only in proteins containing more than one polypeptide chain. The amino acids in human bodies are made it two different ways: either from scratch or by modifying others. A few amino
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suffer from problems such as enzyme leakage from matrix when the interactions are relatively weak. Nonspecific Adsorption The simplest immobilization method is nonspecific adsorption, which is mainly based on physical adsorption or ionic binding. In physical adsorption, the enzymes are attached to the matrix through hydrogen bonding, van der Waals forces, or hydrophobic interactions; whereas in ionic bonding the enzymes are bound through salt linkages. This method is the easiest method of preparing
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with the building blocks of proteins: tryptophan. Using a chemical reactor tryptophan hydroxylase, cells build serotonin by merging with tryptophan. “The precursor of serotonin is the amino acid tryptophan. The enzyme tryptophan hydroxylases adds a hydroxyl group producing 5-HTP. The enzyme 5 HTP decarboxylase removes a carboxyl group from 5HTP and the result is 5HT or serotonin (Carlson, p.121)”. Serotonin is stored in synaptic vesicles where it awaits its release. These synaptic vesicles are located
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For the material; we had a box for housing flies, a lock to lock the locker containing the box for housing flies, group lab books, vials, foam plugs, fly-nap, netting, anesthetizing wand, media contain yeast and antifungal agent, plastic cups, paintbrushes, index cards, vial labels, record sheets, and Carolina Drosophila. Then we had to get ready the Drosophila fly vials; to finish these eight actions are essential. Phase one add 1.5 cm of flakes into vial to about 1.5 cm high of the vial. Phase
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Materials. Minimum essential medium alpha modification (α-MEM) with L-glutamine (2.0 mM), penicillin-streptomycin, trypsin ethylene diamine tretaacetic acid (EDTA), 6- thioguanine (6-TG) were purchased from Sigma-Aldrich. Fetal bovine serum (FBS) was purchased from Hyclone (Logan, UT). Dimethylsulfoxide (DMSO) and potassium chromate (K2CrO4) were purchased from Fisher Scientific. Reagents such as crystal violet, 70% ethanol were used as is. Cells were stored in liquid nitrogen until ready for use
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In the experiment “Enzyme Jelly Lab”, it was discovered that the hypothesis was rejected. The hypothesis being: If temperature affects enzyme function then fresh pineapple is the best to use when making jello. The rationale behind this hypothesis was that the bonds within the collagen of the fresh pineapple could withstand the temperature up to 70 degrees Celsius. This would allow bonds to repair themselves after cooling. When combined with hot water, the helices form a 3-D structure and is unraveled
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In microbiology lab different tests are performed for the diagnosis of different disease .they are used different techniques for the detection of presence of microorganism which are causing harmful disease Different test performed are • Test performed by ELISA technique a. Hepatitis B,C , b. Rheheumatoid arthritis (RF FACTOR ) c. Treponemapallidiumhaemagglutination test (TPHA) • Test performed by latex test a. CRP ( C reactive protein test ) b. ASO(anti - streptolysin o ) • Flocculation
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of Acid Phosphatase Activity Under a Variety of Environmental Conditions Introduction: Biological enzymes are very important as they allow the human body to function and produce a wide variety of biochemical reactions that help sustain life. The main purpose of this lab is to understand the narrow optimal range and varying perimeters that allow these enzymes to optimally function. The enzyme, acid phosphatase, and the substrate p-nitrophenylphosphate were subjected to several different conditions
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