An experiment to show effect of the temperature on the action of an enzyme Abstract The experiment was to analyse what reaction temperature would have on Amylase enzyme. We heated alpha amylase solution to set temperatures then tested for the presents starch with iodine solution. Any starch would turn the iodine black. Once the starch had broken down the iodine would remain brown to suggest the presents of maltose. Usually I would expect to find that the reactions would increase as the temperature
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Diagnostic and Biochemical Tests for Gram Positive Cocci/ Gram Negative Bacilli By: Angelita A. Briñas, RMT I. CATALASE TEST + result vigorous effervescence II. COAGULASE TEST III. MANNITOL SALT FERMENTATION TEST IV.DNASE TEST * * Staphylococus aureus on the left is negative for DNase production; the Serratia marcescens on the right is positive for DNase production as evidenced by the area of clearing around the growth. IV. NOVOBIOCIN TEST Rapid
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consistently performed, and more so in recent years, where significant advances in enzyme engineering has fastened the place of such developments. Targeted improvements aim at enzymes with enhanced thermal and operational stability, improved specific activity, modification of pH-activity profiles, and increased product specificity, among others. This has been mostly achieved through protein engineering and enzyme immobilization, along with improvements in screening. The latter has been considerably
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Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell
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something that went well was that the program and technique used to find the data and graph of the results was the same between part one and part two. One example of something that did not go well in the lab investigation was that the amount of H₂O₂ and catalase was not consistent between part one and part two. Another example of something that did not go well in the liver lab experiment was a different liver and a different O₂ sensor was used. Also, the O₂ sensor was put in the Nalgene bottle at different
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the peroxidase became affected within the testing environments. In terms of the enzyme concentration, both of the different conditions (double and half) resulted with the absorption readings at 470 nm to steadily increase over the course of 5 minutes. This result was caused because there were more enzymes present to react with the two substrates, hydrogen peroxide and guaiacol, therefore affecting the overall enzyme activity to become higher. Moving onto the temperature group, the results were a
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(notes or textbook can be used as sources) Peroxisome – Small organelle that is enclosed by a membrane; it makes use of Oxygen (molecular form) to oxidize organic material. As a result of their functions, some of its enzymes produce hydrogen peroxide as a by-product, while other enzymes have the capabilities to degrade it. Plant peroxisome – Peroxisomes that are specific to plants. They perform as well as aid in the processes of many plant cultivations such as detoxification. Arabidopsis
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Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell
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Chapter 3 Enzymes Learning Outcomes Candidates should be able to: (a) explain that enzymes are globular proteins that catalyse metabolic reactions; (b) explain the mode of action of enzymes in terms of an active site, enzyme/substrate complex, lowering of activation energy and enzyme specificity; (c) [PA] follow the progress of an enzyme-catalysed reaction by measuring rates of formation of products (for example, using catalase) or rates of disappearance of substrate (for example, using amylase);
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UTAR FHSC1214 Fundamentals of Cell Biology Trimester 1 How YOU can do well in BIOLOGY Follow the 4A’s and you can expect A’s. A ttitude • Attend ALL lectures, tutorials and practicals on time without fail. • Be attentive in class and revise your notes after class while the topic is still fresh in your mind. Why waste time re-reading 2-3 months later? • Do your assignments faithfully as they carry marks for the finals. • Come prepared for lessons (i.e. read up beforehand). •
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