The purpose of this lab was to genetically transform bacterial plasmid DNA with foreign pieces of DNA, pGLO, that causes expression of an ampicillin resistant gene, and see the effects it has on bacterial growth in presence of the antibiotic, ampicillin. We inserted pGLO DNA into the genome of the bacteria through the use of inoculation loops, Laurel Broth, transformation solution, and procedures such as heat shock and incubation. This experiment involved four bacterial agar plates, two of which
Words: 1796 - Pages: 8
the experiment was to see how abrasion works and how it affects rocks and minerals with the use of sugar cubes. I measured the mass of all 5 sugar cubes together then placed it in a jar with a lid on and shook it 20 times, as I was shaking the jar my lab partner recorded the mass that was taking with the number of times I shake the jar. After which we drew the shape of the sugar cubes when it was poured out on a paper, then we calculated the percent change. This procedure was repeated 5 times and with
Words: 462 - Pages: 2
When 3-nitrophthalic acid, a dicarboxylic acid, is combined with hydrazine and heated, 3-nitrophthalhydrazine, a cyclic diamide is produced. The nitro group is then reduced with sodium dithionite, producing luminol. The nucleophile in this reaction is the nitrogen of the amine and the electrophile is the carbonyl carbon of 3-nitrophthalic acid. Since the carbonyl carbon is not a good electrophile, under normal conditions, the two reagents will not react. In this case, Fischer’s approach of running
Words: 803 - Pages: 4
The Effect of Aerobic Exercise on the Cardiovascular and Respiratory System Anatomy & Physiology of Sport and Exercise Task 2: Laboratory Report BTEC Extended Diploma in Sports and Exercise Science Year 1 Contents Page 1 Front Page 2 Contents Page 3 –8 Introduction 9 Method 10-11 Results 12-18 Discussion Introduction The hypothesis for this project is to look at the effects of exercise on the cardiovascular system (heart rate and
Words: 4111 - Pages: 17
Introduction Lever is a very simple device that can measure the mass of an object. In this experiment, we are going to make a lever that consists of ruler, 50 gram weight and string that could measure the mass of a battery. We will get the mass by using the formula of moment (moment = distance×force) and based on the data that we obtain from the experiment. Moment is a force that can make object turn around if there is a pivot, for example the see-saw. Objective * To understand the
Words: 626 - Pages: 3
Phys261-203, the Capacitor Author:. 09/24/2015 Instructor: Swabir Silayi Abstract: In this experiment, we are going to investigate the relationship between the distance between two parallel plates and the capacitance, and the how the material of the plates impact the capacitance of the capacitor. In the first part, we are going to set vary the distance between two plates at interval of 0.5cm from 0.25cm to 4 cm to get sufficient data. In the second part of this experiment, we are going to
Words: 1551 - Pages: 7
What is the effect of enzyme concentration, pH level, and temperature on the rate of reaction? Theresa Lashinski Annandale High School In partial fulfillment of the requirements for College Biology Mrs. Kraemer November 27, 2012, 2012 Abstract What is the effect of enzyme concentration, temperature, and pH levels on the rate of reaction for the enzyme tyrosinase? An experiment was conducted, manipulating the pH levels, temperature and concentration of the enzyme tyrosinase
Words: 1490 - Pages: 6
associated with this lab was if the strength of the SPF in four different types of products would prevent or continue the growth of light sensitive yeast when tested. The yeast is essentially our skin and the growth of the yeast shows that the SPF of the product tested was effective. If the yeast growth was muted, then the SPF of the product did not protect our skin. Background information: Based on the information regarding depth of penetration of sun rays, we formed a lab that allowed us to
Words: 1693 - Pages: 7
INTRODUCTION: The kidneys are bean-shaped organs in the abdominopelvic cavity that are found just beneath the rib cage. The kidneys are vital in that they keep the components of the blood stable, which gives the body a chance to continue working properly (McConnell, 1982). About one to two quarts of urine are produced each day by the kidneys (McConnell, 1982). Urine leaves the kidneys and travels to the bladder through two muscles called ureters. Once the bladder is completely filled with urine
Words: 1416 - Pages: 6
Introduction: Photosynthesis is one of the most fundamental processes that take place in plants and some other organisms. Photosynthesis converts solar energy into chemical energy of a carbohydrate (Mader & Windelspecht, 2013).The whole process takes place in the chloroplasts of green plant cells containing the pigment chlorophyll. Chlorophyll is one of the most important molecules in existence as all energy capture starts from there (Mader, 2010). If none of this pigment is in existence, plants
Words: 1544 - Pages: 7
