Screening of various plants viz; Oreganum vulgare (Oregano), Ocimum basilicum (Basil), Cymbopogon citratus (Lemongrass), Corriander sativum (Corriander), Mentha piperita (Mint), Trigonella foenumgraceum (Fenugreek) and Aloe barbadensia (Aloe Vera) have been screened for the presence of protease enzyme. 4.1 Screening of various plants for protease enzyme Protease has been extracted from various plant samples and assayed for enzyme activity using standard protocols as mentioned in materials
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Addition Sequence B(ml) S(ml) T(ml) Working reagent Distilled water Uric acid Standard (S) Sample 1.0 1.0 1.0 0.02 -- -- -- 0.02 -- -- -- 0.02 Mix well and incubate at 37oC for 5 min. or at R.T. (25oC) for 15 min. Measure the absorbance of the standard (Abs.S), and Test Sample (Abs.T) against the Blank, within 30 min. Calculations Uric Acid in mg/dl = Abs.T X 8
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Investigation 2 Paragraph 1 Based on the results of our photosynthesis experiment, we can state that each treatment of light, varying in intensity of the light source, were not significantly different from each other. The positive control at 2 feet away from the light source when compared to the treatment of higher intensity of light at 1 foot away from the light source had a p-value of 0.1778. The positive control when compared to the treatment of lower intensity of light at 3 feet away from
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thus showing an expected linear relationship between temperature and mass-specific metabolic rate. The metabolic rate of an ectotherm can be measured by analyzing the oxygen intake per unit time per unit mass, using a respirometry system. In this lab the metabolic rate change of an ectotherm, a chameleon, was measured through indirect calorimetry. Indirect calorimetry refers to a set of known conditions to which an organism is exposed (Buffenstein, Lemons, Levitt & Hanks, 2015) in a closed chamber
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Bacteria can be found in plants and animals, in all habitats, on all surfaces and even in our bodies. To investigate the type bacteria existing in different locations, we took a culture from the inside of a mouth and the surface of a tabletop and let the bacteria grow in agar plates for one week. Separate agar plates were prepared for each of the two locations that also contained a small amount of two different cleaning solutions, Lysol and Green disinfectants, to observe which product worked better
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Introduction Water can diffuse across the partially permeable membrane of cells depending on the concentration difference between the inside and the outside of the cells. You are going to look at the effect of different concentrations of sugar solutions on potato cells. By measuring the mass of the potato cylinders, you will be able to find out if water has entered or left the potato cells. Aim To find out what happens to the mass of potato cells when they are left in increasingly concentrated
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Fourier Transform Infrared (FTIR) Spectroscopy Group 1 (Monday 1-5pm) Author Reshma Reji Outline Introduction Objective of the Experiment Theory * FTIR Spectroscopy * FTIR Spectrophotometer * Samples Experimental Physical properties of reagents used Solution Preparation Procedure Instrument settings Data 1. IR spectrum of Chloroform and D-chloroform 2. Rotational Spectrum of CO2 (Standard Resolution) 3. Rotational Spectrum of CO2 (High Resolution)
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Keywords: HPTLC, Tetracyclines, residues and broiler meat, validation Introduction Tetracycline antibiotics (TCs) which are represented by oxytetracycline (OTC) and tetracycline (TC), are active against gram positive and negative bacteria antimicrobials and are commonly used in livestock and poultry for prevention and treatment of various diseases [1]. Chemical names for these antibiotics are 4-(Dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro3,5,6,10,12,12a-hexahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide
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Discussion: The purpose of this lab was to investigate the effects that the intermolecular forces of different sized alcohols had on temperature decrease during evaporation. This was investigated using a thermometer to measure the initial temperature of a cotton ball containing 10 drops of an alcohol and its final temperature after 3 minutes of evaporation. It was hypothesized that Butanol, the largest molecule and the one with the strongest bonds, would experience the least temperature decrease
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INTRODUCTION The human population relies heavily on the oxygen in the air in order to survive every day. The oxygen that humans rely so heavily on is produced by plants in a process known as photosynthesis. Photosynthesis can be understood by simply looking at its equation. The equation for photosynthesis states that the reactants of carbon dioxide and water, with the use of solar energy, produce carbohydrates and oxygen. Photosynthesis is broken up into two types of reactions, the light reaction
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