Caffeine was isolated from tea by a sequence of solvent extractions of hot water and dichloromethane. The solid- liquid extraction, which involved hot water, removed the caffeine from the tea leaf and left behind cellulose and soluble organics. The lab actually began with the liquid- liquid extraction that involved dichloromethane and tea, and partitioned components in the layers based off their solubility in the two solvents. Since caffeine is moderately polar, it dissolves in hot water but more
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In the lab, All That Glitter, students learned to calculate and differentiate the density of different forms of matter. Students calculated the density of pre-1982 pennies, post-1982 pennies, and an unknown metal sample. In order to complete the lab, students first took ten pre-1982 and post-1982 pennies and carefully dried them. Afterwards, the pennies were placed on a digital scale in order to find the mass. Students then found the volume of the pennies by filling the graduated cylinder with
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To start the lab, the wire was cut into two pieces, both about 12 inches in length. The wire was burnt from one end on each of the wires to expose the copper end, one wire was burned an inch and half the other an inch. Crayfish was selected from the box and the claws were taped off and placed it into the pan. Using the needle provided two small holes were made through the cephalothorax. One hole will be on the upper cephalothorax where the copper wire with one inch burned is placed. The other hole
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We extracted Daphnia magma from an open container environment. The Daphnia that where used measured 4-5 millimeters is length from head to tail. To set up the experiment, a clear acrylic 30 X 2 centimeters (cm) mesocosm was as our testing environment. The mesocosm was washed out with warm water before being filled with purified water until it is 3-4 cm away from the top and 10 Daphnia were added to the tube and then a cork was added. A line was drawn at 10 centimeters from the cork at one end and
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David Chavez Arturo Huipe Chem 221B Lab January 20, 2016 Experiment 1: Introduction to Microscale (Option A) 1. Weigh a 3-mL conical vial with the screw cap and Teflon insert on a scale. Weight it to the nearest milligram (nearest 0.001 g) 2. Use the automatic pipette and get 0.500 mL of water and put it into the vial, replace the cap assembly (with the insert arranged Teflon side down), and weigh the vial a second time. 3. Find the weight of water dispensed. 4. Determine the density of water
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Safety #1 2. Obtain a sample of food and a food holder. Mount the food onto the food holder so that it can burn without damaging the holder. Find and record the initial mass of the food sample and food holder. CAUTION: Do not eat or drink in the laboratory. 3. Connect the Temperature Probe to the computer interface. Prepare the computer for data collection by opening the file “01 Energy in Food” from the Biology with Vernier folder of Logger Pro. 4. Set up the apparatus shown generally
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The following lab (Rubisco Western Blot) procedure was done over a course of three weeks. During Week 1, gels were prepared, plant extracts were produced and the Bradford Assay was completed. First 2 identical gels were poured. This was done by assembling 2 sets of glass plates, inserting combs between the plates, and using a permanent marker to mark a line 1 cm below the teeth of the comb. The comb was then removed. Vinyl Gloves were put on for protection from acrylamide due to the toxicity of
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Six sheets of Parafilm "M" laboratory film was used and obtained from Neenah, WI. Gel Seal ¼ oz tube was used and had the ID number 80642143. A corks metric ruler was used. A black plastic wedge was used. A 60-cc and 10-cc syringe was used. Sample treatment buffer (STB) was used
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To start the lab each student was required to have on a pair of goggles, long pants, and an apron on at all times. First, start by receiving four sterile nutrient agar plates then writing on each perimeter of the agar plates, with a sharpie, the student's initials, instructor name, class period, and the product being tested on the bottom of the agar plate. Be sure that the bottom of the agar plate stays facing up. Next, get two sterile cotton swabs and dip the tips of each swab into the culture tubes
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& Func on Mitosis Meiosis DNA & RNA Mendelian Gene cs Popula on Gene cs 3 4 Lab Safety Always follow the instruc ons in your laboratory manual and these general rules: eScience Labs, LLC. designs every kit with safety as our top priority. Nonetheless, these are science kits and contain items which must be handled with care. Safety in the laboratory always comes first! Lab Prepara on • • Please thoroughly read the lab exercise before star ng! If you have any doubt as to what you
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