Thermophilic Bacteria of Yellowstone National Park CEE:5154 Environmental Microbiology Research Paper University of Iowa Department of Civil and Environmental Engineering December 14, 2015 Bruce McWilliams Amid the vast, sparsely populated regions of Northwest Wyoming, lies one of the most diverse and extraordinary ecosystems in the world, Yellowstone National Park. Yellowstone is one of the world's foremost sites for the study and appreciation of the evolutionary history of the earth
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International Journal of Biological Sciences Impact Factor ISSN 1449-2288 News feeds of IJBS published articles Manuscript Status/Login Contact 6 May 2015 Home Index & Ranking Current Issue Archive Cover Images Editorial Board Author Info Submission Special Issues Contact Journal of Genomics now in PubMed/PubMed Central. Submit manuscript... Top Introduction Materials and Methods Results Discussion Supplementary
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mitochondrial CO1 genes with the BLAST library, it was revealed that DNA bar coding is convenient and accurate for taxonomy study. DNA bar coding utilizes the amplification and purification of a specific region of the mitochondrial genome by polymerase chain reactions (PCR). DNA bar coding then uses the PCR products ran in gel electrophoresis to analyze. Materials and Methods— DNA Isolation The wild card specimens were obtained and brought to the lab for DNA isolation. The first step in DNA isolation
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I. 진단혈액 진단혈액 수련항목 (1) : 혈액학적검사 기본 술기 표준수련기간 : 1주 수련내용 : ◆ 용어정의 : • 혈액학적검사 : 혈액세포와 응고에 관련된 일련의 검사를 의미한다. 혈구의 체내분포, 구조, 기능에 관련된 검사, 골수에 분포하는 전구세포에 관한 검사, 혈구에 영향을 끼칠 수 있는 혈장 인자에 관한 검사 및 유전자 이상에 관련된 검사 등을 포괄적으로 포함한다. • 망상적혈구수 : 적혈구 성숙 단계 중 정염색성 적아구(orthochromatophilic normoblast) 바로 다음 단계의 세포로 핵이 빠져나간 직후부터를 의미한다. 미토콘드리아, 중심소체(centriole), 리보솜 등을 함유하고 있으며 말초혈액에서 24-48시간의 성숙과정을 거쳐서 성숙한 적혈구로 된다 (Ref. Williams 16th p373-374) ◆ 숙지할 필수 지식 : • 혈액학 검사에 사용되는 검체와 항응고제의 작용기전 및 종류
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DNA band near the top of the gel is the nicked circular plasmid. The nicked circular has one DNA strand nicked by cellular topoisomerase during replication (Tirabassi, 2014). Hence, the DNA superhelical tension is relaxed allowing access for DNA polymerase. The resulting plasmid is a large and loose circle with slowest mobility in the gel. The following band, the linear plasmid is formed when both strands of DNA helix is cut at one site by restriction enzyme and is often due to contamination of endonuclease
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Learn Genetics Gel Electrophoresis Virtual Lab 1. How is it possible to sort different lengths of DNA strands using gel electrophoresis? 1. Shorter strands travel faster, thus causing them to be farther down the gel. Longer strands travel slower, so they will appear near the top of the gel. 2. List the steps required to complete the gel electrophoresis process. 1. Make the gel 2. Set up gel apparatus 3. Load the DNA sample into the gel 4. Hook up the electrical current and run
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the nearest hundred bp) the sizes of PCR products your group obtained from amplification of DNA from homozygous wild type flies, homozygous mutant flies and heterozygous flies. • Comment on the result obtained from the “no template control” PCR reaction. Bioinformatics analyses (< 1 page, 25 marks) • Compare the actual PCR results with those predicted by the bioinformatics analyses you performed using “Primer-BLAST” program. Discuss any differences. • Summarise the outcomes of your “nucleotide
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Purpose To examine the microbial population of an elliptical at the Dixon Recreation Center by analysis of DNA from an uncultured community sample. 16S rRNA genes will distinguish bacteria and represent the microbial diversity of the sampled elliptical. An estimate of the cleanliness of the elliptical will be made based on species-abundance as well as evidence of any pathogenic strains. We will also compare and contrast respective populations of bacteria on clean and disinfected ellipticals to assess
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PRIMER DESIGN QUIZZ Basing on the properties of primer for PCR process, a primers are designed to satisfy most of the requirements to be a good primer: The forward primer: 5’- VR THGAGTWYTA YRTYRTVG - 3’, the template from nucleotide 399 to 418 The revere primer: 3’- G TGAARTYVCG HACCSKN - 5’, the template from nucleotide 609 - 627 1) The reason for the region chosen: There are two basic reason for the region chosen for primer design: - Firstly, in this region, there are a high similarities
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1 执行总结 ................................................................................. 4 1.1 1.2 1.3 1.4 1.5 1.6 项目背景 ....................................................................................................... 4 项目分析 ....................................................................................................... 4 公司与战略 ................................................................................................... 5 市场分析 .................................................
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