Agarose Gel Electrophoresis for the Separation of DNA Fragments Pei Yun Lee, John Costumbrado, Chih-Yuan Hsu, Yong Hoon Kim Department of Molecular, Cell, and Developmental Biology, University of California Los Angeles Video Article Chapters 0:05 Title 1:31 Preparation of the Gel 3:21 Setting up Gel Apparatus and Separating DNA Fragments 4:55 Observing Separated DNA Fragments 5:43 Results: Agarose Gel Electrophoresis of PCR Products 6:23 Conclusion Cite this Article Lee, P. Y.
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Anaerobic bioremediation of groundwater contaminated by chloroethenes Introduction Chloroethenes, such as tetrachloroethylene (PCE) and trichloroethylene (TCE) are one of the contaminants in soil and groundwater which is a significant problem domestically and internationally. Without extracting these contaminants from the ground, the in-situ treatment which directly degrades contaminants in soil or groundwater is now closely watched as an economical remediation technique. Especially, the
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Lecture 8 Nucleic Acid-Based Measurements Text Chapter 13 Wednesday, July 24, 2013 Total community DNA • Extract DNA from soil – – – – remove cells from soil separate cells from soil lyse cells separate DNA from cells – purify DNA • Extract DNA from soil – Extract DNA from cells in presence of soil • Bead-beating • chemical or enzymatic treatment – Sodium dodecyl sulfate or lysozyme Wednesday, July 24, 2013 DNA purification • Cesium chloride gradient centrifugation • Kits
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Introduction: Vitamin D is critical for embryonic skeletal formation and long bone growth. Without vitamin D, the hypertrophic cell zone will expand because the cartilage fails to calcify leading to rickets. Protein disulfide isomerase A3 (PDIA3), also known as ERp60, ERp57, Grp58, and 1,25-MARRS, is a multifunctional protein that has been associated with rapid membrane-initiated signaling by 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3) in several cell types. Knockdown of PDIA3 in osteoblast-like
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DNA in Forensic Science DNA is the carrier of genetic information in humans and other living organisms. It has become a very useful tool in forensic science since it was discovered. In forensic science, DNA testing is used to compare the genetic structure of two individuals to establish whether there is a genetic relationship between them. One example of the use of DNA in forensic science that is important in biology today is comparing a suspect’s DNA profile to DNA that was discovered at a crime
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contents including: forward and reverse primers, template DNA, and Q5 Hot Start High-Fidelity 2X Master Mix (which contains a polymerase with 3’->5’ exonuclease activity, dNTPs, and Mg2+). Towards the conclusion of lab, the PCR tubes containing the contents necessary for a successful PCR were added to a thermocycler. The thermocycler’s role is to provide strict control over the reaction temperature and duration of exposure to each distinct temperature, this ensures the amplification process. We also poured
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Student number: 3003481………………… DNA Paternity testing Two rams at the Easton College farm, a Norfolk Horn sheep and a Southdown sheep escaped from their field and ran in the ewes’ enclosure. The ewes were all Suffolk breed, an old native British breed with excellent lamb growth rate. You were asked to help with identifying the father of the lambs and you decided to try a DNA paternity test with two lambs. http://www.norfolkhornbreeders.co.uk/history.php I Protocol To perform a quick
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GENOMIC TANDEM REPEATS AND DNA FINGERPRINTING In the human genome, sequences of repeated nucleotides are repeated in tandem hundreds of times at dozens of different regions throughout ones genome. DNA profiling uses these numerous repetitive sequences as a method of identifying individuals. These repetitive sequences, called variable number tandem repeats (or VNTRs) and short tandem repeats (STRs) are highly variable and thus form excellent tools for genetic individualization. CLASSES OF GENOMIC
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Once the sample is processed, the samples are tested for the presence of various pathogenic viruses. In my placement laboratory this is usually done by using the real time fast PCR instrument. This is a quick and reliable method, generating results by the end of the day. However, since the principle of PCR is molecular amplification of nucleic acid, it has a disadvantage of getting contaminated quite easily. If a sample even gets slightly contaminated by a few virus particles or previously amplified
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ABSTRACT Real-time quantitative PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistical analysis of the enormous amount of data gained by this technology, as these functions
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