AccuData® GTS/GTS
The Advantage test strip contains the enzyme glucose dehydrogenase which converts the glucose in a blood sample to gluconolactone. This reaction liberates an electron that reacts with a mediator. The oxidized form of the mediator hexacyanoferrate (III) accepts the electron, forming the reduced form of the mediator, hexacyanoferrate (II). The Advantage test strip employs the electrochemical principle of biamperometry. The monitor applies a voltage between two identical electrodes, which causes the reduced mediator formed during the incubation period to be reconverted to an oxidized mediator. This generates a small current that is read by the monitor.
If controls do not fall within the acceptable range after one repeat
1 Do not report the patient results (this would be if the operatoris running controls for the purpose of checking the meter; otherwise, the QC lockout would not have allowed the patient to be tested prior to getting the controls in acceptable range).
2 Review the test policy and procedure.
3 To get a trade, take all of the reagents and supplies out of the drawer and take the entire GTS to the High Volume Lab of Chemistry, 5th floor McCullough Bldg.
4 If feasible for the testing site, the Nurse Manager/designee or Clinic Manager can authorize the borrowing of a GTS unit from another location. cont. NOTE: The proper comment code (09) identifying a temporary GTS unit is being used should be entered after the patient result is displayed and before it is "entered" by the operator. Point of Care should be notified that the assigned GTS unit is not performing satisfactorily.
5 Control testing should be repeated with the borrowed monitor, and, if acceptable, patient testing can be performed and reported.
6 If the problem cannot be resolved with a borrowed monitor, a patient sample should be collected and sent to the clinical laboratory for testing.
7 Roche Diagnostics (the Accu-Chek manufacturer) can be reached 24 hours/day at the technical services help line (1-800-440-3638). If any instrument repairs are needed, or a trade is sent, Roche will send the replacement parts to the lab for repairs.
LIFESCAN SURESTEP FLEXX
PRINCIPLE:
The test employs a dry reagent technology based on the glucose oxidase method that is specific for D-glucose. When a small drop of whole blood is applied to a SureStepPro test strip, glucose oxidase on the test strip triggers the oxidation of glucose in the blood sample. Gluconic acid and hydrogen peroxidase are produced as a result of this reaction. Peroxidase on the test strip then causes the hydrogen peroxide to react with dyes to produce a blue color in the presence of oxygen. This blue color is visible through the confirmation dot on the back of the test strip---the darker the blue, the higher the glucose level in the blood sample. SureStep brand blood glucose meters measure the color intensity of the confirmation dot and report a plasma-calibrated glucose result.
If the result is "FAILED", press Enter Notes and choose the appropriate comment(s) to document your corrective action:
(1) Procedure Error
(2) Shook Control
(3) Repeated Ctrl
(4) Used New Ctrl
(5) New Strip Vial
Tosoh's advanced HPLC systems are fully automated systems that rapidly and precisely separate haemoglobins found naturally in human blood.
This technique is a form of column chromatography used frequently in biochemistry and analytical chemistry. It involves passing a mixture containing the “analyte” through a column (stationary phase), by a liquid (mobile phase) at high pressure.
Cation exchange chromatography is a process that allows the separation of the mixture based on the charge properties of the molecules in the mixture. Cation exchange chromatography retains analyte molecules based on coulombic (ionic) interactions.
The stationary phase surface displays negatively charged functional groups that interact with positively charged cations in the mixture.
It rapidly and precisely separate haemoglobins found naturally in blood. Charged haemoglobins and other haemoglobin components are eluted at varying times depending upon the net charge of the molecule in relation to a gradient of increasing ionic strength passed through a non-porous cation exchange column
