...Fermentation and Cellular Respiration How will Temperature Affect the Rate of Fermentation? Amy Smith Biology Lab 121 November 21, 2013 Partner: Courtney Sargent Professor: Dr. Marietta Wright Introduction: Fermentation is a process that yeast uses to make carbon dioxide. This occurs in an anaerobic setting filled with fluid (Vullo and Wachsman, 2005). This type of fermentation produces energy. Through glycolysis some of glucose is broken down, but the molecule that is formed cannot go into the Krebs cycle. Instead, it enters another pathway that does not utilize oxygen. This pathway produces 2 molecules of ethanol and 2 molecules of carbon dioxide. Fermentation does not completely breakdown glucose; it does not release much energy that can be captured in the form of ATP (Vullo and Wachsman, 2005). Two molecules of ATP are produced from each glucose molecule. This a low yield compared to Cellular Respiration 32-34 molecules of ATP. The ability to perform fermentation allows the yeast to survive and grow in environments where no oxygen exists. The process of fermentation is a very important process in everyday living. Without it we wouldn't have beer, bread, wine, cheese, yogurt, and vinegar (Mader and Windelspecht, 201-210). Some of these products are less important than others but they are all important in some aspect or another. The purpose of this lab is to have one control test and then change one objective of the exercise for the experimental group, by doing...
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...Biology 13A Lab #12: The Respiratory System Lab #12 Table of Contents: • Expected Learning Outcomes . . . . 94 • Introduction . . . . . . 95 • Activity 1: Structures of the Respiratory System 96 • Activity 2: Measuring Respiration . . . 96 • Activity 3: Film: “Deadly Ascent” . . . 100 [pic] Introduction The organs of the respiratory system include the nose, nasal cavity, sinuses, pharynx, larynx, trachea, respiratory tree, and lungs. They function to transport air to the air sacs of the lungs (the alveoli) where gas exchange occurs. The process of transporting and exchanging gases between the atmosphere and the body cells is respiration. The process of taking in air is known as inspiration, while the process of blowing out air is called expiration. A respiratory cycle consists of one inspiration and one expiration. The point of respiration is to allow you to obtain oxygen, eliminate carbon dioxide, and regulate the blood’s pH level. Respiration rate (breaths per minute) and depth (volume of air inhaled and exhaled with each breath) varies due to changes in blood chemistry that are monitored by the brain. For example, when you exercise, demand for oxygen increases because the cells require more ATP. In turn, more carbon dioxide is produced by cells and diffuses to the blood. The rise in carbon dioxide leads to a decrease in pH, causing the blood to be more acidic. The brain is especially sensitive to pH levels; as pH levels...
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...Course: Bio 102 Instructor: Marina Yoffe Assignment: Lab report (Introduction and discussion) Introduction The way in which different organisms acquire oxygen in exchange for carbon dioxide has truly evolved. The exchange of carbon dioxide for oxygen is essential for the survival of organisms that need to produce the energy molecule ATP through the process of cellular respiration. Respiration (breathing) is the way organisms exchange gases with their environment (White and Campo 2004). During cellular respiration organisms break down energy rich macromolecules such as starch to make ATP in a chemical reaction where oxygen and water is involved (Ege R. and A. Krogh 1914).The use of oxygen is very important; therefore the efficient acquisition of oxygen is very necessary for an animal’s survival. Depending on what type of organism is involved, our sources of oxygen are different and occur through diffusion. Terrestrial animals obtain oxygen through the air while organisms living in water depend on the oxygen from the water. Unlike terrestrial animals, the amount of oxygen for animals living in water is limited based on the amount of oxygen that has dissolved in the water. Since an adequate amount of oxygen is necessary, animals developed respiratory organs to help them ventilate and obtain as much oxygen from their respected sources as possible (W.C and R. Oesting 1934). One organism that has developed an efficient way of acquiring oxygen is fish. Fish use a counter-current exchange...
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...Alcoholic Fermentation Accompanied by Dry Mustard Biology Lab (Section 10) 17 November 2012 Abstract Alcoholic fermentation of Saccharomyces cerevisia has been used for centuries in baking. The process of alcoholic fermentation by the Saccharomyces cerevisia creates byproducts such as carbon dioxide and ethanol. The byproduct of ethanol that Saccharomyces cerevisia produces is used in winemaking and brewing. Yet, in baking the yeast reacts to the heat which produces large amounts of carbon dioxide gas that causes the dough to rise. This happens since the gas can’t escape, the pressure from the gaseous molecules make the dough expand. With this knowledge, an experiment procedure can be conducted to test for activators or inhibitors for yeasts production of carbon dioxide. Introduction Fermentation is an anaerobic, meaning without the use of oxygen in the process; this is vital for organisms. When oxygen is low, organisms can no longer perform cellular respiration. Fermentation allows the organism to continue to make ATP molecules, but in lower increments that still allow organisms to maintain function. Because fermentation does not use oxygen, it only involves the prior process of glycolysis (Reece). This experiment specifically deals with the alcoholic fermentation since the yeast used is Saccharomyces cerevisiae. It is a single-celled fungus, also known as “baker’s yeast,” that is most common in alcoholic beverages and bread. Alcoholic fermentation of Saccharomyces...
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...Marlena Ferone LeafLab Biology 101 - Professor Landry University of Phoenix Due Date: August 30, 2011 EXPLORATION What is the relationship between the increase in light intensity and the photosynthetic rate in leaves from a corn plant? How does this relationship compare with what you observed for tomato plants? As light intensity increases, the photosynthetic rate of a plant increases as well. However biochemical reaction, in fact, DO have temperature limits because if the temperatures reach a certain degree and overheat, the enzymes will get damaged and/or lost and inevitably die. Therefore, temperatures must be a tolerable limits in order to increase a photosynthetic rate. Photosynthesis at low temperatures actually take CO2 more efficiently. The rate of photosynthesis is decreased by higher oxygen concentrations. Lower temperatures inhibit Rubisco and forces this enzyme to to work competitively. Rubisco works competitively because is actually binds itself to oxygen rather than binding to carbon dioxide because of the mere attraction. Therefore, in doing so, this activates photorespiration rather than photosynthesis. Photosynthesis in corn is different from photosynthesis in a tomato. Corn is part of CO4. CO4 plants are actually able to capture CO2 into precursor acids. In doing so, CO2 is kept at a constant rate. Increases and decreases in temperature do not affect the rate of CO2. Corn is able to continue efficient energy production which surprisingly allows it...
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...Lab 6: How to Write a Lab Report (1) Atta, S., M. Ikbal, A. Kumar, and N. D. Pradeep Singh. 2012. Application of photoremovable protecting group for controlled release of plant growth regulators by sunlight. Journal of Photochemistry and Photobiology B: Biology 111:39-49. --This article shows that sunlight is the key requirement for plant growth. It explains how sunlight helps in releasing plant growth regulators in plants which enhances plant growth. This article is helpful for my lab report because we provided sunlight to our plants which makes their development faster. (2) Ikram-ul-Haq, Z. A., G. M. Taseer, M. U. D. Mukesh, and S. Ali. 2011. Effects of different fruit juices used as carbon source on cucumber seedling under in-virto cultures. African Journal of Biotechnology 10:7404-7408. --This article shows how fruit juices help plants to grow better because of the sugar that fruit juices contain. They used strawberry and apple juices, but they were not as good as orange and grape juices, regarding their effect on plant growth. This article is helpful for my lab report because we used orange juice to measure its effect on the growth of our plants. (3) Einset, J.W. 1978. Citrus tissue culture: stimulation of fruit explants cultures with orange juice. Plant Physiology 62:885-888. --This article shows the effect of orange juice on plant growth. It explains how using high concentrations of citric acid does not enhance the plant growth, but using...
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...ENERGY IN A CELL Lab Name: Tigist Badeg Data Table. Step | Tile | 1. | Energy | 2. | H2O, CO2, Chlorophyll | 3. | Electron Transfer | 4. | ATP + NADPH | 5. | Calvin cycle | 6. | Glucose and O2 | 7. | Glycolysis | 8. | Pyruvic acid | 9. | Krebs cycle | 10. | ATP, H2O and CO2 | 1.What is the importance of the light and dark reactions in photosynthesis? In light reactions, light is absorbed by chlorophyll in the thylakoid membrane and energizes the electrons. ATP is created from ADP and P. NADP accepts electrons and turns in to NADPH, which is energy. Once the light reactions have taken place, the light-independent, or ‘dark’ reaction occurs in the stroma, where CO2 is converted to sugar. The dark reaction entails the Calvin Cycle, where CO2 and energy from ATP is utilized to create sugar. (Mader, 2009) 2. What happens to food energy during photosynthesis? During cellular respiration? During photosynthesis, plants are actually making food for themselves by reducing the carbon dioxide in the air to carbohydrates after converting energy from sunlight (solar energy) into chemical energy. Living cells need to go through the process of cellular respiration to get energy from the food they eat in order to move, reproduce and function. Respiration releases the chemical energy stored in glucose, which is used by the cells to carry out their life activities (growing, moving, etc.). The process of cellular respiration is completed...
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...Rate of Photosynthesis Tyler Marshall AIU Biology Lab (SCIE2017 -1602A-16) * Purpose * The purpose of this lab is to find out the rate of photosynthesis. By measuring the number of oxygen bubbles using different light bulb watts. * Introduction * Photosynthesis and respiration are reactions that complement each other in the environment this much me we know. Even though they are the same thing the difference is that they happen in reverse. What happens is during photosynthesis; carbon dioxide and water yield glucose and oxygen. Also during respiration, glucose and oxygen yield carbon dioxide and water. As of a result of these two things the plants will give off oxygen. Which when in water you will be able to see the process happening through the release of bubbles. * Hypothesis or Predicted Outcome * The more light the plant is exposed to the higher the rate of photosynthesis. * Methods * In the lab we measure the rate of photosynthesis by exposing seaweed in a bowl of water to different light bulb watts. The watts where 0,25,50,100. The seaweed was exposed to each what for 1 hour, three different times. This was done in order to get an average of number of bubbles released to measure the rate of photosynthesis. Also in order to keep the experiment controlled for accrete results. * Results or Outcome * The experiment started out with exposing the seaweed to a light bulb with 0 watts. And after all three 1 hour trails...
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...Associate Level Material Scientific Method Virtual Biology Lab: How Does Acid Precipitation Affect Trees? Please note that the questions below are found electronically in the lab. You are required to provide your answers on this word document instead of answering them in the virtual lab since the electronic submission of your answers from the lab will not reach your instructor. As you work through the lab, it will ask you to answer certain questions. Answer the questions that are required and submit this word document to your instructor. 1. What are probable sources of acid precipitation in your community? What impact can this have on the environment in your region? In my community we have a paper mill that produces a lot of chemicals and delivers pollutants in the air, I live at least 9 miles from this particular mill and I can smell the chemical pollutants it releases from my house. The impact that it has is that it is possibly killing trees and animals in the area, as well as the plants and crops that the farmers grow. 2. What factors apart from acid rain could be killing trees? Oil spills, areas experiencing droughts, trash and litter, and human beings. 3. Limestone, an underlying material for many soils, dissolves and corrodes when exposed to high concentrations of hydrogen ions. How is this information important in understanding the effects of acid precipitation? Because when the limestone corrodes it produces...
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...Photosynthesis BIOS-135 Foundation of Biology & Chemistry with Lab Week V INTRODUCTION In this lab Photosynthesis is a process used by plants and other organisms to convert energy from the light, the most common solar light into chemical energy that can be later released to fuel the organisms' activities. And this happen on plants. Photosynthesis can be represented using a chemical equation. The overall balanced equation is... 6CO2 + 6H2O ------> C6H12O6 + 6O2 Sunlight energy Where: CO2 = carbon dioxide H2O = water Light energy is required C6H12O6 = glucose O2 = oxygen As light energy (in the form of photons) reaches a plant, chlorophyll molecules forming a light harvesting complex absorb that energy, exciting electrons. These electrons move along an electron transport chain, eventually transferring their energy into the bonds of ATP and NADPH. ATP and NADPH act as highly charged energy carriers ready to provide energy to continue photosynthesis in the dark reactions. (University, 2014) In the photosynthesis we have to different processes the light dependent process and the light independent process.in the light dependent process, or photo reduction, is the first stage of photosynthesis, in this process plants capture and store energy from sunlight, In this process light energy is converted into chemical energy, in the form of the energy carrying molecules ATP and NADPH. In the light-independent reactions, the formed NADPH and ATP drive the...
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...Table of Contents: FHSB 1214 FHSC 1214 Biology I Cell Biology Introduction Practical 1 Practical 1 Cell Biology Biological Studies I molecules I Practical 2 Practical 2 Cell Biology Biological Studies II molecules II Practical 3 Cell Biology Studies III Practical 4 Cell Biology Studies IV Practical 8 Cell Biology Studies VIII Practical 5 Cell Biology Studies V Practical 6 Cell Biology Studies VI Practical 7 Cell Biology Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages...
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...Table of Contents: FHSB 1214 FHSC 1214 Biology I Cell Biology Introduction Practical 1 Practical 1 Cell Biology Biological Studies I molecules I Practical 2 Practical 2 Cell Biology Biological Studies II molecules II Practical 3 Cell Biology Studies III Practical 4 Cell Biology Studies IV Practical 8 Cell Biology Studies VIII Practical 5 Cell Biology Studies V Practical 6 Cell Biology Studies VI Practical 7 Cell Biology Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages...
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...Introduction It has been determined that the rates of chemical reactions are affected by temperature. This experiment is done to test the effect of five different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. “The fermentation rate is measured in ml/min.” The purpose for performing this experiment is to understand the rate of fermentation of yeast cells which can be determined by measuring the amount of carbon dioxide produced at each temperature. Since there is no direct way to measure the carbon dioxide produced in the lab, an indirect method is used instead. The fermentation rate in this experiment is measured in ml/min which is the rate of carbon dioxide production that is measured over time spent in each water bath. Water will substitute for the carbon dioxide produced at the end of the experiment. The amount of water is measured in milliliters (by the graduated cylinder) will serve as an indirect method of fermentation rate because carbon dioxide is a gas which cannot be measured by means other than indirectly with this experiment. Carbon dioxide production was measured by measuring the volume of water at different temperature; 25°C, 35°C, 45°C, 55°C, and 65°C. The time was also recorded at which all the measurements were taken for the different temperatures. Materials and Method The materials used in this experiment was: Yeast culture (contains yeast and glucose in the form of molasses) Five fermentation tubes...
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...Describe one weakness or area of growth that you overcame in our class and how you overcame it. Before I entered AP Biology this year, I was really self-critical of my own work. My self-esteem was not at the level I wanted it to be, and I deemed any success that I had as a fluke. Throughout the year, I gradually grew more confident about my own work. I would have to attribute biology to being one of my favorite subjects and my willingness to help others. I was so eager to learn more biology this year, and inevitably I had the opportunity to help others. I became progressively more confident about my abilities to say the correct answer and produce high quality assignments and projects not just due to chance, but because of my dedication to learning the...
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...AP Biology Exam Review: Lab Essays At least one essay (FRQ) on the exam will be based on an AP laboratory. To prepare for this question, review the objectives for all twelve laboratory exercises. The College Board does not necessarily expect that you have completed that lab, but rather that you have investigated the objectives of the lab. You may be asked to “design an experiment to determine….” You don’t necessarily need to create a new lab; if you have done an activity that would answer the question, simply describe it. For a good response, you should include the following. 1. State a hypothesis [as an “if…..(conditions), then….(results)” statement] Be sure your hypothesis is testable. 2. Identify the variable factor. 3. Identify the control. Be certain to explain the control for the experiment. 4. Hold all other variables constant. 5. Manipulate the variable. 6. State how you would measure the results. 7. Discuss the expected results. Relate the results to your hypothesis. 8. Include steps to replicate or verify. You may be asked to graph data. Be sure to use a graph that is appropriate for you data. Bar graphs are used when data points are discrete (not related to one another), while line graphs are used with the data are continuous. If there is a data point at zero, be certain to extend your line to 0, but do not extend the line to 0 if there is no data point at zero. Other points to keep in mind: ...
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