...OF EXPERIMENT: EXTRACTION WITH ACID AND ALKALINE OBJECTIVES: 1) Performed on acid-alkaline extraction 2) Learned to recovered benzoic acid and p-dichlorobenzene from its mixture using acid-alkaline extraction method 3) Determine the percent recovery of benzoic acid and p-dichlorobenzene 4) Determine the melting point of recovered benzoic acid and p-dichlorobenzene INTRODUCTION: Extraction involves dissolving a compound or compounds either from a solid into a liqiud or from a solution into another liquid. An acid-alkaline extraction is also known as a form of liquid-liquid extraction. Extraction is a procedure to separate compounds based on their relative solubility in two different immiscible solutions. Two layers are formed when the mixture are separated. The upper layer contains a low dense solvent. Basically in most of the extraction, one of the solvent used would be water as an aqueous solution and the other would be an organic solvent that is insoluble in water. The choice of organic solvent must be volatile so it can easily by removed by evaporation when forming crystals. The cleanest separations of organic compounds can be performed by using acid or alkaline solutions which can convert the compound to be extracted to a water-soluble and ether insoluble salt. Phenolic compounds undergo similar salt formation with sodium hydroxide solution. Hence, sodium hydroxide solution can be used to extract a carboxylic acid or phenolic compound from its solution...
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...students, eugenol was isolated from clove oil via chemically active extraction, using acid-base chemistry. This isolated compound was analyzed via Infrared Spectroscopy and Gas Chromatography. Eugenol, eugenol acetate and caryophyllene (Figure 1) were isolated from ground cloves via steam distillation. To do this, 5.04g of ground cloves were added to a 500mL round bottom flask with 40mL of dH2O, boiling stones and 3 drops of an antifoaming agent (to prevent violent boiling). The contents of the flask were heated using a heating mantle for 75 minutes. The distillate was condensed through a water jacket...
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...Acid and Base Extraction Finding the unknown compound by extraction using their conjugate acid and base. Huda Introduction The purpose of the acid and base extraction lab is to determine the unknown mixture that contains three compounds which is made of an organic acid, an organic base and an organic neutral. To find out what the unknown compounds are found in the mixture, a series of techniques are done. To extract the compounds from the given unknown mixture, they were changed back to their conjugate acids by adding a strong acid and back to their conjugate bases by adding a strong base. The first technique is called extraction in which organic Bases and organic acids are separated from neutral compounds and from each other by using an aqueous solution of different PH values. Compounds that contain less than six carbons are not affected by changing the pH value. The compounds are extracted based on the solubility properties of the acid, bases and salts. The unknown compound will be dissolved in dichloromethane and HCl will be added and extraction will occur by mixing the solution and removing the aqueous layer. The extracted aqueous layer will be isolated by adding NaOH which will make the solution basic that will lead it to precipitate. The organic acid will be extracted NaOH to the dichloromethane solution and separating the aqueous solution. The majority of organic carboxylic acids are highly soluble in dilute NaOH since the acid gets deprotonated by the base and...
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...components. The steam distillation is a special kind of distillation that is sensitive to temperature and very much important in natural (organic oil) extraction. It works on the substance that are volatile and water insoluble. Because the clove oil have boiling point of 2540 C, this method becomes fundamental and necessary for the clove oil extraction as it reduces the boiling point of the clove oil to below 1000 C (Harding, 2008). In the presence of steam, the boiling point temperature of the clove oil reduces to 980C which is volatilized. The vapor mixture made up water and oil is passed through the condenser which cools the vapor that is collected at the end as distillate. Separation using organic solvent is mostly utilized in pharmaceutical industry. The organic molecule in plants after steam distillation would be easily separated by this method. This separation process has high demand energy in the processes involved e.g. evaporation and condensation (Harding, 2008).. The product of steam distillation is to be mixed with diethyl ether to remove traces of water. The product of clove is then tested for phenolic compound. The processed clove oil is mostly used as an antiseptic and is processed by the help of steam distillation of organic compounds. The cloves oil has eugenol, eugenol acetate and β-caryophyllene as the constituent organic compounds which vary depending on the cloves...
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...Organic based solvents are used commonly in the world of drug synthesis, however their residues are undesirable. Many techniques are used to remove them due to the fact these residues can negatively alter the drugs in pharmaceuticals. This is due to their toxicity, influence on the quality of crystals, and their odor or taste which can be unpleasant for the patients that are receiving the drug.1 Although manufacturing techniques are available to get rid of the solvents, not all of the solvent is removed. The small quantities of these organic solvents that remain are designated as residual solvents or (RS).1 (ptfarm)Even though these residual solvents are in such miniscule quantities, these compounds can still affect the quality of the final product....
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...3. INTRODUCTION Organic compounds are isolated either from natural sources or from reactions mixtures. These compounds are seldom pure and are usually contaminated with small amounts of other similar compounds, which are found to exist together or formed during the reaction. In order to characterize them, it is important to purify them. 4. PROCESSES FOR PURIFICATION 1. Crystallization 2. Sublimation 3. Distillation 4. Chromatography 5. Differential Extraction 6. Qualitative Analysis 5. 1. CRYSTALLIZATION Aim To separate a solid compound in pure and geometrical form. Principle A saturated solution of the impure substance in a suitable solvent is made at a temperature higher than the room temperature. On cooling this solution, the substance reappears in the form of well shaped crystals. Process Purification by crystallization involves the following steps: Choice of solvent Preparation of solution Filtration of the solution Separating the crystals Drying of crystals Example Crystallisation of Phthalic acid 6. 2. SUBLIMATION Aim To separate volatile solids, which pass directly into vapour state on heating from a non-volatile solid. Principle A mixture of solid substances, such as camphor, benzoic acid, ammonium chloride, iodine etc., containing non-volatile substances, when heated, change directly into vapour without passing through the liquid state. Process 7. Fig :-Sublimation 8. 3. DISTILLATION Aim To separate a solution of a solid in a liquid and for separating a solution...
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...Lab report for Experiment #2: Extraction Your Name: Name of TA: Lab Partner’s Name: Lab Section: Title: Experiment #2: Extraction Purpose: What is the purpose of this lab? In your OWN words! Observations: Weighed out 3.2568 grams of chemical mixture that was yellow in color. Dissolved dry chemicals in 38 ml CH2Cl2 with gentle heating. Poured the yellow solution into sep funnel. Added 10 ml CH2Cl2 to flask to rinse, poured solution into sep funnel. Added 15 ml 3 M HCl, gently mixed and vented (saw bubbles during mixing, heard the evolution of gas while venting). Allowed layers to separate. Upper layer determined to be aqueous by density: lower layer removed. Upper layer placed into separate flask labeled “A” for acid extract. Lower layer placed into sep. funnel and reextracted with 15 ml 3 M HCl. Combined aqueous layers in flask A Reextracted CH2Cl2 layer containg chemicals with 15 ml 3 M NaOH. Upper layer in flask labeled “B” for base extract. Re-extract organic layer with 15 ml 3M NaOH. Combined base extracted materials in flask “B”. Saw bubbles during mixing, heard the evolution of gas while venting. Dried organic layer with anhyd. Na2SO4 until free flowing salt was observed (about 3 grams). Filtered the organic solution into a tared 100 ml round-bottom flask. Rinsed flask and funnel with 10 ml of CH2Cl2 and rotovaped off remaining solvent and weighed flask. The biphenyl obtained was white in color and was like a powder. Weight of flask and biphenyl...
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...hydrocarbons (PAHs) are a group of toxic organic compounds. These organic contaminants are often byproducts of the processing of crude oil or incomplete combustion consisting of many aromatic rings joined together. (1) Occurrence of Polynuclear Aromatic Hydrocarbons in Foods PAHs tend to be Lipophilic in nature and there occurrence in foods can be linked to contamination by soil, water and/or air or from cooking practices at home such as roasting, grilling smoking etc.(2) High temperatures and exposure to sunlight decreases the concentration in food substances. There are over 500 PAHs but only 15 have been identified that affect foods. Figure 1 shows a list of the fifteen PAHs along with their structures. (see appendix) Sources of airborne PAHs are forest fires and volcanoes. The most important stationary anthropogenic sources include residential burning of wood, oil, gas and charcoal as well as industrial power generation, incineration, production of aluminium, iron and steel, petroleum catalytic cracking and production of asphalt, coal tar and coke. The most important mobile sources are vehicular exhausts from gasoline and diesel-powered engines. In combustion processes the formation of PAHs is reduced when combustion is more thoroughly performed but this will increase the formation of nitrogen oxides. . Formation of PAHs The formation of PAHs in foods is varied based on the processing method chosen. The Formation of PAHs compound occur at temperatures of 100-150˚C. It...
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...Experiment 7: Lipid Extraction I. Abstract Lipids are biomolecules that are used primarily for structural components of the cell, signaling molecules and energy storage purposes. Lipids are naturally occurring esters of long chain fatty acids with both hydrophobic chains, which is insoluble to polar organic solvents and hydrophilic chains which is soluble to polar organic solvents. Because of this conformation, they can assume a wide range of complex structures including fused rings. Lipids can be isolated from cells through different techniques and their presence can be tested through different qualitative tests. The sample choice is egg yolk and was used as a source of lipids in the experiment. Liquid-liquid extraction, separation of the organic and aqueous layer was used to extract the supernatant or extract. Also, thin layer chromatography or TLC was used to separate the different lipid components by using the Rf values computed. The farther the distance traveled by the compound (higher Rf), the more nonpolar the component, while the smaller the distance traveled, the more polar the component (lower Rf). Lecithin and cholesterol was not able to travel the plate. After, the isolated lipid was subjected to qualitative tests such as Acrolein test, test for phosphates, Leibermann-Burchard test and test for unsaturation. Acrolein tests determine the presence of glycerin; the test for phosphate detects phosphate groups in the structure of the lipid, Leibermann-Burchard uses cholesterol...
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...nitrogen, which has a lone pair. Although each hydrogen bond is weak compared with a covalent bond, large numbers of hydrogen bonds can have very significant effects. One example of this is in the properties of water. This highly unusual liquid plays a major role in making living systems and the earth’s environment behave as they do. Carbon normally forms four bonds, and carbon-carbon bonds are quite strong, allowing formation of long chains to which side branches and a variety of functional groups may be attached. Hence the number of molecular structures which can be adopted by organic compounds is extremely large. Functional groups containing oxygen atoms, nitrogen atoms, and multiple bonds often determine the chemical and physical properties of carbon compounds. Therefore organic chemistry may be systematized by studying related groups of compounds such as alkanes, cycloalkanes, aromatic compounds, alkenes and alkynes, alcohols, ethers, aldehydes and...
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...EXTRACTION Concepcion, Ma. Noelle. U., Corpus, Ian Dexter. N., Dayanghirang, Vivien Angela I., De Vera, Yerim K., Del Pilar, Nicole Alexandre L. Group 3 – 2CPH Organic Chemistry Laboratory ABSTRACT The group extracted caffeine from tea using single extraction, where 10 tea bags(15g) were boiled in 100ml of water containing 4.4g of anhydrous sodium carbonate. The extract was placed in a separatory funnel and the 60 ml of dichloromethane was poured at once to the mixture in a separatory funnel. From two distinctive layers, the clear portion was collected in a beaker containing anhydrous sodium sulfate. The group decanted the solution to pre-weighed evaporating dish and was evaporated to dryness. The weight of the dried product was 0.9 g, which was determined by subtracting the weight of pre-weighed evaporating dish from weight of evaporating dish containing the residue. The percentage yield of the caffeine was 6%. INTRODUCTION: Extraction is the process of solute tra-nsfer from one solvent to another solvent to where the extractive is more soluble (Pavia, 1998). The theory of extraction is the immiscibility in two liquid phases to separate only desired content from the others. It is used in the isolation of natural products, which consist of multi-component mixtures, from its sources. It commonly utilizes organic solvents, such as ether, hexane, and benzene or methylene chloride to extract organic substance. Extraction consists aqueous phase and organic phase,...
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...structures of DNA. Caffeine is found in numerous applications and can stimulate different parts of the body, possibly causing addiction, insomnia, and headaches. Using the proper extraction methods, the caffeine within a tea bag could potentially be isolated to yield a pure solid; the mass of this solid would reflect the actual yield of caffeine in the tea. Introduction Caffeine belongs to a large class of organic compounds called alkaloids, which vary widely in structure and reactivity. These nitrogen based compounds are found in plants and often taste bitter 2. Alkaloids can be found in different forms such as nicotine, morphine, or cocaine. The structure of caffeine is shown below along with the purine ring. Figure 1: Structure of purine and caffeine Caffeine is found in a number of foods and beverages. Such has chocolate, soda, and over the counter medication. The purpose of this experiment was to isolate caffeine that is found in tea by using the techniques of extracting, separating, drying, and sublimation. To do so, caffeine must be introduced to a solvent that is both volatile and insoluble to water; a perfect example is dichloromethane. Caffeine has a greater affinity for dichloromethane and will easily dissolve in this solvent over water; however, caffeine is not the only organic substance found in tea that is capable of reacting with dichloromethane. Along with caffeine, tea contains another substance, tannins, which are capable of dissolving in water as...
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...EXTRACTION OF ESSENTIAL OIL FROM MURRAYA KOENIGII LEAVES USING ULTRASONIC-ASSISTED SOLVENT EXTRACTION METHOD ZA ISKANDAR B MOHD DIKUI UNIVERSITI MALAYSIA PAHANG EXTRACTION OF ESSENTIAL OIL FROM MURRAYA KOENIGII LEAVES USING ULTRASONIC-ASSISTED SOLVENT EXTRACTION METHOD ZA ISKANDAR B MOHD DIKUI A report submitted in partial fulfillment of the requirement for the award of the degree of Bachelor of Chemical Engineering (Gas Technology) Faculty of Chemical Engineering & Natural Resources Universiti Malaysia Pahang APRIL 2009 v ABSTRACT The objective of this research is to extract essential oils from M. koenigii leaves by using ultrasonic-assisted solvent extraction method. The major constituent of M. koenigii has been reported as caryophyllene and 3-carene which is responsible for the aroma and flavor. This research has focused on the influence of ultrasonic, various natures of solvents, sonication times and also drying method towards the extraction of M.koenigii essential oil. Two types of solvents are used in this research which is ethanol and hexane. In this research, the methods of drying, grinding, extraction, separation and analysis are used and the sample is separated from solvents by using a rotary evaporator to get the essential oil. The sample was analyzed by using a GC-MS to identify the component of M. koenigii essential oil. In this research, the most suitable solvent to produce higher percentage yield is by using ethanol...
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...there was little to no separation between the organic compounds, the impurities remained in the distillation flask. The impurities were later removed before extraction. Extractions were performed to further purify the distillate of the alcohols and sulfuric acid. Water was able to remove the leftover alcohol from the solution, which resulted in the water spikes in our NMR and IR spectrums. Water was able to remove the leftover alcohol because water is a polar molecule and the hydroxyl groups are attracted to the layer that is more polar. On the separatory funnel, there were three layers, organic layer (product layer), aqueous layer, then once again the organic layer. The product was formed on both the top and bottom layer even though they were only supposed to form on the bottom layer of the separatory funnel because the products are denser than water. If the organic layer converged at the top of the aqueous layer, it indicates that the reaction did not proceed as much as it should have and contained alcohol. Since the product was at both top and bottom layer, it means that some of the reagent was went through the reaction like it was supposed to and has no alcohol, but some reagents did not and contained some alcohol. The product was then washed with sodium bicarbonate to remove the acids in the organic layer, which produced salt and carbonic acid, which decomposed to water and CO2. The polarity of the C-Br bond allowed the organic layer to cling onto water molecules after the...
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...CHEMISTRY CHEMISTRY (CLASSES XI–XII) Rationale Higher Secondary Stage is the most crucial stage of school education because at this stage specialised discipline based, content oriented courses are introduced. Students reach this stage after 10 years of general education and opt for Chemistry with a purpose of mostly for pursuing their career in basic sciences or professional courses like medicines, engineering, technology and studying courses in applied areas of science and technology at tertiary level. Therefore, at this stage, there is a need to provide learners with sufficient conceptual background of Chemistry, which will make them competent to meet the challenges of academic and professional courses after the higher secondary stage. National Curriculum Framework for School Education – 2005 recommends a disciplinary approach with appropriate rigour and depth with the care that syllabus is not heavy and at the same time it is comparable to the international level. It emphasizes a coherent focus on important ideas within the discipline that are properly sequenced to optimize learning. It recommends that theoretical component of Higher Secondary Science should emphasize on problem solving methods and the awareness of Syllabus for Secondary and Higher Secondary Levels 22 historical development of key concepts of science be judiciously integrated into content. The present exercise of syllabus development in Chemistry at Higher Secondary Stage is based on this framework...
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