...Properties of Gases Peter Jeschofnig, Ph.D. Version 42-0189-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor. Observations Make a table of the gases studied. Tabulate their colors, the effect on lighted or glowing splints, and other properties one might use to identify them. | | |Data Table: Experiment Results | |Gas |Flame |Glowing |Limewater |Bromothymol | | |reaction |splint |reaction |blue reaction | |Hydrogen |Bright orange | | | ...
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...MLT1 Experiment 5/Task 6 Differential Staining There are many different ways to stain bacteria for viewing under a microscope. For the most part these are categorized as “either simple, nonspecific or differential (specific) (LabPaq, p.128). The most common staining method used is Gram staining. Gram staining is a differential or specific method of staining. Gram staining is a way to tell the difference between gram positive and gram negative bacteria. The cell wall of the gram positive bacteria is made up of several “layers of peptidoglygan,” and “techoic acids,” (LabPaq, p. 128). This peptidoglycan is what absorbs the color part of the crystal violet stain causing the gram positive bacterial cell to appear violet colored when viewed with a microscope. In contrast, the cell wall of the gram negative bacterial cell is not as thick as that of the gram positive cell wall. Peptidoglygans are on the inside of the cell rather than in outer layers. The outer part of the gram negative cell is made up of phospholipids and lipipoly-saccharides (Betsy and Keough, 2005). The outer cell wall does not hold onto the violet color of the crystal violet and appears pink in color when viewed under a microscope. The purpose of iodine staining is to aid the bacteria to keep the stain by creating an iodine-crystal violet mixture that will not dissolve. Iodine is also known as a mordant in this case. A mordant is usually an inorganic oxide that when mixed with...
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...Motility Testing Cynthia Alonzo, M.S. Version 09-2.01 Read the entire experiment and organize time, materials, and work space before beginning. Remember to review the safety sections and wear goggles when working with chemicals. Objectives: To learn flagellar structure and arrangements common in microbes, and To use direct observation and testing to determine if a given microbe is motile. Materials From: Label or Box/Bag: Qty Item Description: Student Provides 1 Microscope 1 Paper Clip 1 10%-bleach Solution 1 Paper towels From LabPaq 1 Gloves packages - 11 pairs 1 Immersion Oil 1 Slide - Cover Glass - Cover Slip Cube (4) Culture Media Bag #2 - Refrigerate upon Receipt Culture Media Bag #2 - Refrigerate upon Receipt 2 Agar, 0.4% Motility Test Agar - 8 mL in Glass Tube Inoculation Instruments Inoculation Instruments 1 Inoculation Loop, Plastic Mask Bag Mask Bag 1 Mask with Earloops (11) in Bag 5" x 8" Slide Box MBK Slide Box MBK 1 Slide-Box-MBK with Blank-Slides (4) Pre-Lab Preparation: Place saved cultures of E. coli and S. epidermidis (from previous lab) in incubator 12-24 hours prior to the start of the experiment. Discussion and Review: Many bacteria are capable of motility, the ability to move under their own power. Most motile bacteria propel themselves by special organelles termed flagella. The bacterial flagellum is a noncontractile, semi-rigid, helical tube composed of protein and anchors to the bacterial cytoplasmic membrane...
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...MicroBiology- MLT1 LabPaq / Published by: Hands-On Labs, Inc. sales@labpaq.com / www.LabPaq.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology 50-0222-MB-01 LabPaq® is a registered trademark of Hands-On Labs, Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs, Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s unique design, assembly, and learning experiences. The laboratory manual included with a LabPaq is intended for the sole use by that LabPaq’s original purchaser and may not be reused without a LabPaq or by others without the specific written consent of HOL. No portion of any LabPaq manual’s materials may be reproduced, transmitted or distributed to others in any manner, nor may be downloaded to any public or privately shared systems or servers without the express written consent of HOL. No changes may be made in any LabPaq materials without the express written consent of HOL. HOL has invested years of research and development into these materials, reserves all rights related to them, and retains the right to impose substantial penalties for any misuse. Published by: Hands-On Labs, Inc. 3880 S. Windermere St. Englewood, CO 80110 Phone: Denver Area: 303-679-6252 Toll-free, Long-distance: 866-206-0773 www.LabPaq.com E-mail: info@LabPaq.com Printed...
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...MLT1 TASK 2 Western Governor’s University April 02, 2015 Task: A. The difference between Facultative and Obligate aerobes is that while both aerobes require oxygen to survive facultative aerobes can survive with or without oxygen to survive. Obligate aerobes require oxygen to live and grow organisms, with facultative aerobes can grow live and grow organisms with or without oxygen depending on the certain conditions and organisms. B. According to labpaq (labpaq, received 2015) it stated it was very important to keep the temp at 37 degrees. 37 degrees is a prime temperature for S. epidermis and L. acidophilus bacteria that commonly live on the skin to grow. C. It is very important to control the growth of microbes one way to do this is with adequate aseptic technique. For example cleaning IV poles, blood pressure machines, stethoscopes using “sani wipes” or other disinfectants is appropriate. For proper aseptic technique in control growth on humans, I would use first and foremost proper hand washing, then things like iodine, alcohol, and hibicleanse. D. There are several forms of culture media. “Media can be either liquid (nutrient broth) or solid (agar).” (ScienceProf) “Very few forms of Microorganisms can degrade agar, so it usually remains in a solid form.” (Microbiology, Demystified, 2005) Agar slant puts the test tubes on its side to grow. Agar dishes are plates that lie flat to grow bacteria. “A culture made by inserting an inoculating nee...
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...« Study and create flashcards for free at Cram.com Sign In | Sign Up StudyMode - Premium and Free Essays, Term Papers & Book Notes Essays Book Notes AP Notes Citation Generator More Experiment 4: properties of gases By swifty217, june 2013 | 2 Pages (480 Words) | 76 Views | 4.5 1 2 3 4 5 (1) | Report | This is a Premium essay for upgraded members Sign Up to access full essay DID YOU LIKE THIS? TELL YOUR FRIENDS... Send Report Submitted: June 21, 2013 Title: Properties of Gases Purpose: To analyze a few physical and chemical properties of gases as well as using those properties to identify the gases. Procedure: * For Hydrogen I placed a piece of Zn metal into a test tube with diluted HCl. * To produce oxygen I added Hydrogen peroxide to manganese. * Next, I added baking soda to vinegar. This caused a quick and energetic reaction! The gas from the reaction I pumped into some Limewater. * Then I mixed Alka Seltzer and water and pumped the gas caused by that reaction into some limewater. Data: Data Table: Experiment Results | Gas | Flame Reaction | Glowing Splint | Limewater reaction | Bromothymol blue reaction | Hydrogen | It enlarged the flame. | | | | Oxygen | It lit up quickly | | | | Hydrogen & oxygen | It puts it out. | | | | Carbon dioxide | It puts it out | | It bubbled and turned cloudy. | It turned it yellow | Alka Seltzer | | | Bubbles stayed in the...
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...Background: Acids are substances that are characterized by their ability to donate protons (H+) to other substances in a solution. Acids can be strong or weak. Weak acids do not ionize completely and are found in everyday substances. Acidity is a measure of the amount of dissolved hydrogen ions in a solution. The greater the number of hydrogen ions in a solution, the more acidic the solution. Strong acids donate hydrogen ions in water, to a much greater extent than weak acids. Bases are substances that are characterized by their ability to accept protons or produce hydroxide ions (OH-) in an aqueous solution. As for acids, the difference in strength of bases directly relates to the degree in which hydroxide ions are formed by the base in water. Like strong acids, strong bases are also dangerous chemicals. Weak bases are found in many everyday items. When feeling the consistency of a base, it feels slippery because it degrades the fatty acids and oils in the skin on contact. However, due to the possibility that an acid or a base could be very dangerous to human tissue, never experiment with an acid or base by tasting or touching it. To determine if a substance is an acid or a base, a pH scale may be used. pH is a measure of the concentration of hydrogen ions in a substance. The equation for pH is: pH = -log [H+]. The logarithm for pH is based on a scale of 10. As pH is a negative logarithm, the pH of a substance increases as the concentration of hydrogen ions decreases. For...
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