...The Extraction of Bioethanol from Pineapple (Ananas comosus) Peelings Through Simultaneous Saccharification and Fermentation Using the Yeast Saccharomyces cerevisiae AN INVESTIGATORY PROJECT SUBMITTED AS AN ENTRY TO THE 16TH INTERNATIONAL ENVIRONMENTAL PROJECT OLYMPIAD (16TH INEPO) 16. INEPO ÇEVRE PROJE OLİMPİYADI FATİH KOLEJİ (FATIH COLLEGE) ISTANBUL, TURKEY 1-4 JUNE 2008 Avril Rodiel Bries Quezon City Science High School (Regional Science High School for NCR) REPUBLIC OF THE PHILIPPINES- CUMHURIYET FİLİPİNLER School Year 2007-2008 Mr. Noel Pablo Diaz Research Adviser Abstract: On average, 435,000 metric tons of pineapples are produced annually in the Philippines, which is one of the country’s leading commercial fruit products. However, there are a lot of unused excess parts of the pineapple, notably the peelings, which are considered as waste and contribute to the country’s garbage problem. There is a global oil crisis, as the demand for petroleum increases each year while our supplies are rapidly being depleted. Bioethanol, a principle biofuel, is a natural alternative to gasoline. One solution to both dilemmas is to produce bioethanol from pineapple plant peelings. This will be made possible by extracting the glucose content of the peelings and fermenting it through the process of Simultaneous Saccharification and Fermentation (SSF) using the yeast sample. Peel samples, coming...
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...The genetics of S. cerevisiae Biology 2 Genetics of Saccharomyces cerevisiae Part 1: Introduction & Determination of Phenotypes Overview You will be given three strains of Saccharomyces cerevisiae (named A, B, and C), each with a different genotype. The hypotheses for this study are (1) identify the phenotypes and genotypes of the three strains in regards to their ability to synthesize leucine, and uracil; and (2) determine whether these genes follow Mendel’s Second Law and assort independently. Goals and objectives of this project: • • • • • Learn how to work with S. cerevisiae, a model organism that is powerful for genetic research Experimentally explore genetic concepts (auxotroph vs. prototroph, genes vs. alleles, diploid vs. haploid, complementation, segregation and independent assortment, meiosis vs. mitosis, genotype vs. phenotype) Develop and test scientific hypotheses Learn to work with a microscope, micropipettor, microcentrifuge and hemacytometer Learn about the genetics of metabolism How this project will be graded: 1. Your scores for Question Sets 1, 2, and 3 will be combined into a single grade. That grade will be included in your Lab Average for the course. Your Lab Average will be included in your final semester average as described in the syllabus for this course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 2. Your scores for Question Sets 4, 5, and 6 will...
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...in a cylinder containing ethanol. * We put 50microliters of special broth in starved agar plate containing dextrose and lactose. * We grow them for seven days Independent variable is the yeast plate containing dextrose and dependent variable is yeast plate containing lactose. We plan to look at the sizes of colonies to find out whether there was an increase or decrease in them. This will help us to reject or not to reject our hypothesis. Reference Ratcliff, W. C., Denison, R. F., Borrello, M., & Travisano, M. (2012). Experimental evolution of multicellularity. Proceedings of the National Academy of Sciences, 109(5), 1595-1600. Varon, M., & Choder, M. (2000). Organization and cell-cell interaction in starved Saccharomyces cerevisiae colonies. (Statistical Data Included). Journal Of Bacteriology, (13-14), 3877. Lavrentovich, O. M., Koschwanez, J. H., and Nelson, D. R., (2013). Nutrients shielding in cluster of cells. Biological Physics, (Submitted on 23 Apr 2013 (v1), last revised 13 Jun...
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...UNIT 24: BREWING SCIENCE Get assignment help for this unit at assignmenthelpuk@yahoo.com LO1 Understand fermentation systems Microbial groupings: microbial range and form Unicellular fungi: importance to the fermentation process especially Saccharomyces species History of fermentation advances: metabolic pathways of respiration; dynamics of anaerobic respiration/fermentation; methods of controlling fermentation (top and bottom); principal steps in the brewing process LO2 Understand the biochemistry of malting, mashing and conversions in the copper Barley physiology: choice of cereal; biochemical changes affected by the malting process Wort composition: biochemical/biophysical conversions Malting process: the enhancement of diastatic power; control of N2-content; development of colour Mashing process: the ionic balance of the liquor (Burtonization); factors affecting extraction and enzymatic conversion in the grist; importance of temperature and time controls; monitoring ‘run-off’ from the mash tun for turbidity and specific gravity Hop variety: varieties of hops available (including brief histories and geographical location); characteristic properties of each variety; hop additions to brews (biochemistry of action); hop quality (appearance, feel and aroma) Hop biochemistry: the ratio of α and β-acids; oxidative conversions to humulones and hulupones; the effect of seed content on hop property and the principle contributors to aroma The copper: inactivation of enzymes; precipitation...
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...Case Study: Mountain Man Brewing Company Situation/Background Analysis: Mountain Man Brewing Company (MMBC), located at West Virginia State, was a family-owned beer company, which possessed respectable market shares in the Central East beer region of the United State. Thanks to the unique bitter flavor and the high quality of its only product -- Mountain Man Lager, which was selected as the “Best Beer in West Virginia” and “America’s Championship Lager” recently, the small company gained a national well-known reputation and therefore was able to survive in the fierce competition of the U.S beer market. In addition, the core customers of MMBC, baby boomers, blue-collar, middle-to-lower income and mid-aged men, still shared their ultimate loyalty to the brand and formed a stable, large portion of annually profits to the company. In spite of all the strengths above, in 2005, the company encountered the first decline on products sale during its 80-years history. Based on market researches, the company believed that this decline was induced mainly by two reasons: 1. Over the past 6 years, the traditional premium beer segment to which MMBC belongs had shrunk, due to the growing light beer segment. 2. Mountain Man Lager, as MMBC’s core product, is not popular among younger drinkers who constitute the vital consumer segment for beer companies. Such a decline became a clear alarm to the MMBC management team after the company had seen so many other regional breweries disappear in the...
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...Application Note: 51853 Determination of Free Glycerol in Biodiesel with the Evolution Array UV-Visible Spectrophotometer Nicole Kreuziger Keppy, Gordon Bain, Ph.D., Michael W. Allen, Ph.D., Thermo Fisher Scientific, Madison, WI, USA www.analiticaweb.com.br/newsletter/08/AN51853_Biodiesel_UV.pdf Introduction Key Words • ASTM Standards • Biodiesel • EN Standards • Free Glycerol • Transesterification • UV-Visible Spectroscopy The consumption of biodiesel as an alternative fuel source is on the rise. Biodiesel is used in its pure form or added to fuel blends to power automobiles and heat homes. Biodiesel is typically produced from vegetable oils and animal fats by adding an alcohol, usually methanol, to triglycerides in a transesterification reaction as shown in Figure 1 below. The product of the transesterification reaction is the biodiesel fuel itself in the form of fatty acid methyl esters (FAME) and a glycerol by-product.1 Free glycerol affects the quality of biodiesel and its content is regulated by industry standards. In this experiment, the amount of free glycerol in biodiesel is measured with a Thermo Scientific Evolution Array UV-Visible spectrophotometer for conformance to American (ASTM)2 and European (EN)3 standards for free glycerol using the Quantification Mode of the Thermo Scientific VISIONcollect software. A solvent solution containing a 1:1 ratio of deionized water and 95% ethanol, and a reference solution of 0.036 mg/mL...
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...catalase using a drop of hydrogen peroxide on each organism, prokaryotic and eukaryotic. In the third experiments we will be testing if either of the organism can do fermentation, in different products such as glucose, lactose, and sucrose. Oxidase Test Materials 4 wooden sticks Prokaryotes (Neisseria sicca and Escherichia coli) Eukaryotes (Candida albicans and Saccharomyces cerevisiae) BBL DrySlide Oxidase Test Procedure Gather 4 wooden sticks that will be used for each test tube with different bacteria’s, 1 BBL Dry slide will be used to determine the oxidase reaction of bacteria, 2 test tubes with eukaryotes, and 2 test tubes with prokaryotes. Using a wooden stick gently take small portion, and transfer it onto BBL DrySlide, rub the bacteria onto one of the four slides. Wait patiently approximately 20 seconds for a color change, appearance of blue color indicates a positive reaction, any other color represents a negative reaction. Dispose the wooden stick and DrySlide. Catalase Test Materials 4 wooden sticks Prokaryotes (Neisseria sicca and Escherichia coli) Eukaryotes (Candida albicans and Saccharomyces cerevisiae) 4 glass slides Hydrogen Peroxide (H2O2) Catalase Test Procedure Gather 4 wooden sticks, each stick will be used for each organism; 4 glass slides will be used for each organism, 2 prokaryotes and 2 eukaryotes, and a bottle of hydrogen peroxide to test for the reaction. Using a wooden stick gently take a small portion of the colony and transfer it to one of...
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... 17 November 2012 Abstract Alcoholic fermentation of Saccharomyces cerevisia has been used for centuries in baking. The process of alcoholic fermentation by the Saccharomyces cerevisia creates byproducts such as carbon dioxide and ethanol. The byproduct of ethanol that Saccharomyces cerevisia produces is used in winemaking and brewing. Yet, in baking the yeast reacts to the heat which produces large amounts of carbon dioxide gas that causes the dough to rise. This happens since the gas can’t escape, the pressure from the gaseous molecules make the dough expand. With this knowledge, an experiment procedure can be conducted to test for activators or inhibitors for yeasts production of carbon dioxide. Introduction Fermentation is an anaerobic, meaning without the use of oxygen in the process; this is vital for organisms. When oxygen is low, organisms can no longer perform cellular respiration. Fermentation allows the organism to continue to make ATP molecules, but in lower increments that still allow organisms to maintain function. Because fermentation does not use oxygen, it only involves the prior process of glycolysis (Reece). This experiment specifically deals with the alcoholic fermentation since the yeast used is Saccharomyces cerevisiae. It is a single-celled fungus, also known as “baker’s yeast,” that is most common in alcoholic beverages and bread. Alcoholic fermentation of Saccharomyces cerevisiae creates the byproducts, carbon dioxide and ethanol (Encyclopedia...
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...INTRODUCTION Microorganisms are used extensively to provide a vast range of products and services. They have proved to be particularly useful because of the ease of their mass cultivation, speed of growth, use of cheap substrates (which in many cases are wastes) and the diversity of potential products. Their ability to readily undergo genetic manipulation has also opened up almost limitless further possibilities for new products and services from the fermentation industries (Trevor Palmor, 2004). Microorganisms are a large and disease group that exist as its single cell or cell cultures. These include bacteria, fungi, algae, protozoa and infectious agents at the borderline of life. Microbes are present most abundantly in soil, atmosphere and water plays a important role in the biochemical agent for the conversion of complex organic compounds into simple organic compounds (Glazer and Nikaido, 1995). Microorganisms are closely associated with health and welfare of human beings. Some Microorganisms are beneficial and others are detrimental. For example, Microorganisms are involved in the making of yoghurt, cheese and wine, in the production of Penicillin, interferons and alcohol, and the processing of industrial and domestic wastes. Microorganisms can cause disease, spoil food and deteriorate materials like iron, pipes, glass lenses and wood pilings (Glazer and Nikaido, 1995). Most of these Microorganisms irrespective of their origins, were subsequently modified by conventional...
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...process.Vinegar was produced through two successive fermentations: alcoholic and acetic fermentations. The alcohol fermentation was carried out at 30°C using yeast. Biomass, pH and Brix were evaluated daily during the fermentation. Acetic fermentation was carried out at 30°C using an acetic bacteria strain isolated from pineapple wine previously exposed to ambient temperature (28°C) for 5 days. Biomass, pH and acid levels were monitored each 2 days. The performance of acetic bacteria isolated was also assessed by studying their glucose and ethanol tolerance. The study allowed the isolation of yeast coded Saccharomyces cerevisiae (LAS01) and an acetic bacteria coded Acetobacter sp. (ASV03) both occurring in the pineapple juice. The monitoring of successive fermentations indicated that the pineapple juice with sugar concentration of 20 Brix, seeded with 106 cells of Saccharomyces cerevisiae (LAS01) for alcoholic fermentation for 4 days and afterwards seeded with 106 cells of Acetobacter sp. resulted in 4.5 acetic degree vinegar at Brix 5.3% and pH 2.8 for 23 to 25 days. The study of glucose tolerance of the strain of Acetobacter sp. showed that the growth of acetic bacteria was important in a juice with high concentration of sugar. However, the concentration of ethanol did not effect on the acetic bacteria growth. These results enabled on one hand to improve the manufacturing technology of vinegar from fruits and on the other hand to produce a starter of yeast and acetic bacteria strains...
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...This experiment was designed to test whether yeast is able to metabolize with different types of sugars. In this experiment the four sugars that were tested were milk sugar or lactose (disaccharide), table sugar or sucrose (disaccharide), blood sugar or glucose (monosaccharide), and starch (polysaccharide) . Specific amounts of the four sugars were mixed with a specific amount of the yeast, saccharomyces cerevisiae, separately to test how the yeast respire using different sugars. Yeast are unicellular, single-celled organisms. Yeast has a nucleus, making them eukaryotic (Khan). Yeast is beneficial to humans because it is used in baking bread, beer and wine. The use of yeast in baking bread helps it to expand and rise (Bakeinfo). Beer, wine,...
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...Background Information Like all other living organisms, yeast thrives best in environments that are suited to its personal needs. Factors that determine how well an environment is suited for an organism include the amount of sunlight present, pH level of liquids around them, and the temperature of their surrounding atmosphere. This experiment will be testing the effect of different temperature levels on different categories/types of yeast. Yeast is a one-celled, eukaryotic organism that produces both carbon dioxide and alcohol by taking in sugars and completing alcohol fermentation. As for the different types of yeast, there is active and inactive. Active yeast refers to a strain/species of yeast that is alive. Inactive yeast refers to a...
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...production economics, making them challenging targets for future R&D activities. Moreover, the application of used coconut oil as an alternate carbon source for biosurfactant production would provide an advantage for the development of the productive chain of the coconut, an important industrial segment in tropical coastal regions. B Sridhar et al (2012) studied Purification and Characterization of Biosurfactants. The emulsifications of biosurfactants were checked in various oils such as olive, coconut, sunflower and rice bran oils, the emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa than Saccharomyces cerevisiae. Under this study, after centrigugation of crude extract,the pellets were suspended in a 40 mL of dichloromethane in a separating funnel and shaken vigorously and the allowed surfactant recovered in the organic layer at the top. The extraction performed twice and the organic layers were pooled and evaporated. The organic layers were collected in separate conical flasks (Abushady et al., 2005; Yalcin and Ergene, 2010). It was latter characterized by using thin layer chromatography (TLC). ...
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...Brandy Brandy is an alcoholic beverage produced by the distillation of wine with an alcohol content of between 35 – 60% by volume. It is mostly served as an after-dinner drink, but also popular in bars and nightclubs in many countries. It can also be produced by fruits other than grapes but is sold under different names, such as eaux-de-vie in France and schnapps in Germany. There are varying requirements held by countries in order to classify a drink as a brandy, but the most recognisable characteristic is that the distilled fruit alcohol is aged in oak containers for at least one year. The making of brandy is an effective way to salvage defective wines and production surpluses, though many good quality brandies are made from wines specially grown. Brandies are produced by distillation of wine in a piece of machinery called a rectifier. The wine is distilled in the distillation column where the “phlegm” or main distillation product is isolated. This highly concentrated alcohol product is then extracted while hot and further purified in the next column known as the epuration column by further removal of head foreshots and unwanted compounds, before finally reaching column C – the rectification column. The rectification column is where the potable pasteurized alcohol is drawn off. Some of the separation products during distillation can be recycled into the process, but methanol, due to its toxicity in humans is carefully removed. A disadvantage of this process is...
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...Annotated Bibliography Barnes, Deborah E, Leland H Johnston, K-L Kodama, et al. 1990. Human DNA ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences 87: 6679-6683. CDNA clones encoding for DNA ligase I were isolated. In one method, human cDNA was screened using oligonucleotides from partial amino acid sequence of purified bovine DNA ligase I. and the second approach the human cDNA library was screened for functional expression of a polypeptide able to complement of a DNA ligase mutant of Saccharomyces cerevisiae. The sequence found encodes a 102 kDa protein indistinguishable from DNA ligase I. It was also found that the amino acid sequence of the human ligase I is 40%...
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