How Does Different Amounts of Salt Affect the Water Level? By Caleb Randall Science Block 6 Mrs. Ryan Table of Contents Abstract 3 How Does Different Amounts of Salt Affect the Water Level? 4 Experiment 6 Trial 1 7 Trial 2 8 Trial 3 10 Conclusion 11 BIBLIOGRAPHY 12 Abstract This project was about how different amounts of salt affect how much water will rise. This paper describes how different amounts of salt affect the increase or decrease of the water level. In
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In one method, human cDNA was screened using oligonucleotides from partial amino acid sequence of purified bovine DNA ligase I. and the second approach the human cDNA library was screened for functional expression of a polypeptide able to complement of a DNA ligase mutant of Saccharomyces cerevisiae. The sequence found encodes a 102 kDa protein indistinguishable from DNA ligase I. It was also found that the amino acid sequence of the human ligase I is 40%
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sequence and amino acid sequence of Alpha-B-crystallin, the NCBI protein database was used. Followed by this we utilized the Expasy translation tool to reveal our nucleotide sequence of codons from the mRNA sequence. Our start codon was methionine and our stop codon was lysine. We were assigned to create a mutation of the 159th residue which involved mutating an isoleucine to a leucine. We developed a primer using the Quickchange primer design that involved mutating the first and third base. The primers
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enzymes, also called proteases are a group of enzymes that exist in plants, bacteria, animals etc. One of the common functions of proteases is protein degradation and it is doing so by break protein down into fragments and further into amino acids. The importance of proteases has been realised by recent research as its increased role in biological regulations (Perlmann et al., 1970). Proteases are prominent in tropical fruit with bromelain in pineapple, papain in papaya and ficin in Figs
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29 EXPERIMENT 3. ACID-BASE TITRATIONS: DETERMINATION OF CARBONATE BY TITRATION WITH HYDROCHLORIC ACID BACKGROUND Carbonate Equilibria In this experiment a solution of hydrochloric acid is prepared, standardized against pure sodium carbonate, and used to determine the percentage of carbonate in a sample. An aqueous solution of hydrochloric acid is almost completely dissociated into hydrated protons and chloride ions. Therefore, in a titration with hydrochloric acid the active titrant species is
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standardization of hydrochloric acid To prepare a standard solution of sodium carbonate and use it to standardize a given solution of dilute hydrochloric acid. Anhydrous sodium carbonate is a suitable chemical for preparing a standard solution (as a primary standard). The molarity of the given hydrochloric acid can be found by titrating it against the standard sodium carbonate solution prepared. The equation for the complete neutralization of sodium carbonate with dilute hydrochloric acid is Na2CO3(aq) + 2HCl(aq)
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resulted in minimum production of benzoquinone (product). Even though the original hypothesis was not completely supported, however, as seen in figure 1, the absorbance for the mixture is at its highest when the reaction occurs in the presence of a pH 8 buffer, which is close to the pH 7 originally hypothesized. The catechol oxidase is affected by the different pH concentration, and its most favorable pH value is pH
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1. The different levels of organization are listed as follows: Cell- the structural and functional unit of all living things Tissue – a group of cells with a common structure and function Organ – composed of tissues functioning together for a specific task Organ System – composed of several organs working together Organism – an individual; complex individuals contain organ systems. Population – organisms of the same species in a particular area. 2. 7 basic characteristics of life are
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A Functional Approach: Vitamins and Minerals Understanding Nutrition, 11e Prepared by Michele Grodner, EdD, CHEs NUTRITION STUDENTS: IGNORE THE CHAPTER REFERENCE, THIS INFORMATION IS TAKEN FROM A DIFFERENT BOOK THAN “NUTRITION AND YOU”. BONE HEALTH Bone is active living tissue. Blood travels in capillaries through the bone, bringing nutrients to the cells that maintain the bone structure while carrying away waste materials from those cells. It picks up and deposits minerals as instructed
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through hydrolysis and chromatography. Gluten was isolated then hydrolyzed by proteolytic enzyme and was subjected to different qualitative tests. Paper Chromatography was also performed to analyze the different amino acid components of gluten. Tests showed that Gluten has several amino acid components. INTRODUCTION Proteins are a class of organic compounds which are present in and vital to every living cell. In the form of skin, hair, callus, cartilage, muscles, tendons and ligaments, proteins hold
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