Introduction to Decision Making Methods János Fülöp Laboratory of Operations Research and Decision Systems, Computer and Automation Institute, Hungarian Academy of Sciences 1. Decision Making Process “Decision making is the study of identifying and choosing alternatives based on the values and preferences of the decision maker. Making a decision implies that there are alternative choices to be considered, and in such a case we want not only to identify as many of these alternatives as possible
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hot water (50°C) for use in Part II. 4. Use the marking pencil to label the bottom of one Petri dish S. epidermidis. Pour one half (9mL) of the contents of a tube of nutrient agar into the S. epidermidis Petri dish and the other half into the bottom of an unmarked Petri dish. Cover the dishes and allow them to solidify for use in Part IV. 5. Pour the remaining melted nutrient agar into the unmarked Petri dishes (half a tube per dish). Cover the dishes and allow them to solidify for use in Part
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have been an effective disinfectant for many years used as a treatment for bacterial and fungal infections of the skin and nails (American Cancer Society 2008). Micro-organisms such as bacteria and fungi can be found in a variety of places. Using petri dishes containing nutrient agar, bacteria and fungi can be grown and observed. ‘Agar is a seaweed extract that forms a jelly-like material that is also a source of food for these organisms. Bacteria grow into colonies made up of millions of individuals
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to reduce the likelihood of bacterial contamination? A. Aseptic techniques Q11.Procedure by which Bacteria may be introduced to the media A.innoculation Q12. Culture media is sterilised in A. an autoclave Q13. Solid culture media in a Petri dish is known as . A. agar plate Q14. What is added to culture medium to make nucleic acid and certain types of lipids A.Phosphorus Q15. inoculum is transferred using A. loop Q16. method for transferring micro-organisms onto an agar
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Homemade Petri Plates Commercial microbial media are really best for growing microorganisms. They provide the right nutrients that allow good growth. However, if a commercial agar medium is too expensive, you can prepare homemade gelatin plates that will allow many fungi and some bacteria to grow. This homemade medium can be poured into sterile disposable petri plates or, if those are too expensive, can be poured into foil muffin cup liners that can be stored in plastic sandwich bags. Materials
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(colony-forming units, CFUs), but can be modified for fungi (CFUs) and viruses (plaque-forming units, PFUs for viral counts). A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. The agar solidifies, with the bacterial cells locked inside of the agar. Colonies grow within the agar, as well as on top of the agar and below the agar (between the agar and the lower dish). The procedure described above produces a set of pour
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IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. Instead, biochemical testing has been used to make bacterial identification down to the “species” level. These schemes are based on creating and matching biochemical profiles of the production of enzymes, acids and gases by isolated pure cultures
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1. The streak plate technique is a method of isolation. It is used to produce isolated colonies of organisms using a nutrient medium. In this case, it is used to isolate Serratia marcescens on an agar plate. This technique is used when one needs to separate organisms in a mixed culture. It encourages growth of only one organism. The viable counting technique is used in effort to estimate the number or viable, or living, cells. It relies on cells growing on the nutrient medium, i.e. agar. Multiple
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three-day period. All turkey samples were in a zip locked bag, the 24 and 72 hour samples were left out at room temperature; the control remained refrigerated. All samples were inoculated into agar deeps, then into petri dishes to create a series of dilutions. We observed growth on all three Petri Dishes with the control having the least and 72-hour containing the most. This shows that although the turkey meat was sealed, microbial growth increases over time while left out at room temperature. This is important
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magnifiers, petri dishes, freeze dried ants, tweezers, plastic human bones, pencils, and a science notebook. ADVANCED PREPARATIONS: 1. Go to a pet store to buy a container of freeze dried ants. 2. Bring in Scholastic’s The Human Body by Steve Setford, and National Geographic Kids Ultimate Bug-opedia by Darlyne Murawski and Nancy Honovich. 3. Go to a teacher supply store, or if close to October any retail store, to buy a few plastic human bones. 4. Place a few ants in the petri dishes. 5
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