...Unknown Bacteria Lab Report #21 In Microbiology Lab, we learn the scientific method of streaking, staining, microscopy, aseptic culture, and selective and differential tests. These tests will help me identify my Unknown Bacteria. The first procedure I did was the isolation streak of my unknown out on a Trypticase Soy Agar. The purpose of Trypticase Soy Agar is to provide enough nutrients of the microorganism to grow. After I finish my isolation streak, my plate were incubated and grown. Once I got my plate to look at my results and I realize I started my streaking pattern wrong. Because of the way I started my streaking pattern I wasn’t able to see my own results. However, I did my own research of how my plate should have look like if I had did my streaking right. With an educated guess of my unknown species, the results I found for the plate have colonies agar that are tan- pigment, convex, circular, and smooth. The cells are also motile rods. The colonies can be observed under Ultraviolet Light. The second procedure was to perform a Gram stain. The purpose of the Gram stained method is to differentiate between a Gram positive and Gram negative organisms and also learning about the cellular morphology and arrangements. I carefully prepare a slide smear on three slides and then started the Gram stain procedure. Once the procedure was done, all three of my slides had a pink stain which automatically figured that my unknown bacteria was Gram negative. Then I examine the finished...
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...Lab#5- Ex. 1-5, 2-2, 3-4, 3-5 1. Assume you want to streak a trypticase soy agar plate for isolation using a nutrient broth culture of Staphylococcus epidermidis. A. Indicate how many times you would need to remove the cap from the S. epidermidis broth culture tube, and explain why. B. How many times would you need to flame your inoculating loop, and explain why. Answers: A. The cap is removed one time when I obtain the original sample. B. The loop will be flamed five times; one for each of the four streaks and once after the streak (Leboffe, 2012, p 41-42). 2. Indicate the goal in streaking a plate for isolation, and explain why it is important (Leboffe, 2012, p 41-42). Answer: Streaking a plate for isolation is important because to test and identify the organism correctly it must be in individual colonies or a pure culture (Leboffe, 2012, p 41-42). 3. A. What two cellular characteristics are determined with the negative stain? B. Why is the bacterial smear not heat fixed before performing the negative stain? Answers: A. The two cellular characteristics determined by negative stain is morphology (shape) and cellular arrangement in bacteria that cannot stand heat-fixing (Leboffe, 2012, p 165). B. The bacterial smear is too fragile to withstand heat-fixing. Heat-fixing distorts shape and the cellular arrangement of the bacterial smear (Leboffe, 2012, p 165). 4. Differentiate between cellular morphology and colony morphology. Answer: Cellular...
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...was murdered, but he has had an impact impacted me greatly on the way I choose to live my life. I decided at a young age to never conform to other people's views. My mother will not would not admit it, but she had a plan of how she wanted me to act and look around her friends. On the night of Halloween in 1995, my mother attempted to dress me in a Raggedy Anne costume. Instead of being forced into wearing something I had no interest in wearing, I threw a fit. I screamed and threw my fists until I got to wear what I wanted to, which was nothing but my birthday suit. From adolescence to the present I have tried my hardest to be an individual and keep away from stereotypes. I try to let people know that it is okay to be an individual by making a fool out of myself John Lennon sat in bed for a week to inspire peace and I went streaking through my camp to inspire individualism. I may not have made a worldwide impact, but when I explained to people why I had gone streaking, a few younger campers decided to be fearless and participate in the talent show. (This part is a bit jumpy. I understand you're trying to make a new point, but it doesn't flow too well)Friendship has always meant a great deal to me, and pretty much the entire world. Many people would not take chances if it were not for the push of a friend. Which brings me to my second quote and another reason Another quotation I greatly admire from John Lennon is undeniably one about friendship: I feel that John Lennon and...
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...baleen,oil, and their meat. Whale meat can be made into pet food. Whale meat can also be sold to people too. Whale skin can be made into umbrella’s. Whale oil can be made into lamp oil. Problem and solution Whales have been hunted from 1960s to 1980s for many different reasons.Whales have been hunted for their Meat, Skin, Blubber,Oil and lipstick. they ran at an extreme low amount later on many countries were still hunting the humpback whales this was bad but then they had to think and chose to put them on the no hunting list they still had a hard time to live when Norway,Japanese,Iceland,Greenland,Sloveni,British and were still hunting whales. Until now the population is streaking down to it’s last 30,000 to 40,000 almost 30% of their original population.But at least now the whales population is streaking...
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...ORGANIZATON BEHAVIOR and DEVELOPMENT By: Disha Patel A graduate paper submitted In partial fulfillment of the requirements for the Degree of Masters of Science in Accountancy COLLEGE OF BUSINESS BENEDICTINE UNIVERSITY Lisle, Illinois USA November 18, 2013 Organization Behavior and Development has been a major topic in every business around the world. It is how every organization is built. The environment in an organization is created by organizational culture, behavior, diversity, and communication. The culture in an organization guides the actions of all the employees through shared values and beliefs. Diversity is the differences in race, gender, ethnicity, and how well it is received in the organization. Additionally, communication is also important, if there is an absence of communication it creates barrier between employees, hindering creativity, and disrupting the work environment. The organization that I had worked for in the past is a great example of an organization behavior gone wrong. It is 6:00 AM on Monday my alarm rings, I turn it off and try going back to sleep. When it dawns on me, I am starting my new job today. I get up quickly and get ready to head out. I am so excited and nervous at the same time that I am shaking. It is five minutes before 8:00 AM. I park my car, and head through the employee entrance. As I walk in I see the same woman that I saw on the 16th June 2010 when I came for my interview, I walk up to her and introduce...
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...You look up and see a swarm of people getting into formation. Your anxious. Your body starts to tremble, and your palms start to sweat. You think to yourself, what if I drop it? What would happen if … At that moment someone shouts “Disc in!” Everyone starts fidgeting, and getting ready to make a move to get open. At that second, you breath, plant your feet and begin streaking up field. The wind blows against your face, and you feel another person right behind you. Someone shouts, you turn around and see the frisbee coming right at you. You jump up, and with all your power you manage to and catch it, and land perfectly in the end zone. The feeling of pure thrill, and excitement courses threw your body as your teammates engulf you in happiness. Ultimate Frisbee is an exciting and entertaining sport. Since being created in 1968, frisbees have been seen in countless parks, schools, colleges, and countries. With all its growth and popularity one question arises; Should Ultimate Frisbee be added to the Olympics? In 1968 Joel Silver created what we know of as today to be Ultimate Frisbee. Joel is a famous film producer, who has made countless movies, and short films. But back in 1968, he was a normal high school student living Maplewood New Jersey. Alongside his...
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...n January 16 of this year, the North Carolina Tar Heels were 12-5, having just beaten Virginia Tech in a close game in Chapel Hill. The grumbling old men of Franklin Street were grumbling about what they had been grumbling about for the past two years: Larry Drew II, the team's starting point guard, was playing horribly. In the Virginia Tech game, he had picked up more fouls (4) than assists (3), points (3), or rebounds (2). Kendall Marshall, Drew's freshman backup, had played spectacularly. In 24 minutes, Marshall put up the following line: 3-4 from the field, 2 rebounds, 9 points, 9 assists, and zero turnovers. But statistics, even advanced ones, don't really tell us much about point guard play in college. Yes, Marshall thoroughly outproduced Drew. But when you're the point guard at Carolina, production is a secondary concern. You can shoot badly from the floor, turn the ball over, and have teammates botch assists, but as long as you're pushing tempo and finding streaking and cutting teammates, you're doing your job. Marshall set a torrid pace, but he did it in a strange, almost counterintuitive way. Carolina fans were still dizzied from the memories of Raymond Felton and Ty Lawson — two of the fastest players to ever set foot in Chapel Hill. But Felton and Lawson set pace through more traditional means — they got the ball and ran like jackrabbits up the court. Marshall, who runs with his back as straight as a ramrod and keeps his head up at all times, was much, much slower...
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...Based on the results of my unknown A, I concluded that the bacteria was Escherichia Coli. After I had collected the results and compared them with my dichotomous key, I was convinced that Escherichia coli was the only organism with a bacillus morphology gram negative that was able to survive under this conditions. The result for the lactose fermentation clarified that the bacteria carrie the enzyme lactase in the DNA, therefore can ferment lactose and produce acid and gasses. This type of bacteria contain also the enzyme indole which can use tryptophan as a way for growth and survival. the result from the tryptophanase came out positive, eliminating the identification of the other organisms with a morphology bacillus and with a gram negative...
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...to urinate, burning sensation when urinating, passing frequent, small amounts of urine, urine that appears cloudy, urine that appears bright pink or brown in color (signs of blood in the urine), strong-smelling urine e. Significant additional information. I.e. drug resistance, special growth requirements, vaccines available, etc. (As given by the instructor or found in your reading.) Serratia marcescens is also a common bacteria found in the shower. It is easily identified by the pink color slime. This microorganism shows to have resistance to many antibiotics including: penicillin as well as ampicillin. B. New technique(s) or method(s): (Each technique/method must be written-up separately). 1. What new test/method(s) were introduced during this laboratory? No new test/method was introduced in this lab. 2. Discuss the new test/method(s) used. (See laboratory manual, text, and web source). Why did we perform this test? How do we interpret the...
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...Holcomb, Kansas, is a village containing approximately three hundred citizens. The square town is with described with having rivers, stations, horses, fields of wheat, a bank, and a school. In Cold Blood by Truman Capote, an image of the town of Holcomb is presented throughout types of style such as, diction, imagery, syntax, and tone. In order to communicate a Western way of speaking used in Holcomb, Truman Capote mentions the town as, "out there," and says the pronunciation of the Arkansas River as "Ar-kan-sas." Throughout the town there are a few signs which cause a ghostly presence there. For example, "—Dance—but dancing has ceased and the advertisement has been dark for several years," and "HOLCOMB BANK," which later on said,...
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...British rule unjustifiably taxed the Bostonians tea to a ridiculous price and attempts were made to solve this issue using politics within the British Government. However as the latter says “No Representation” Their voice wasn't heard by the British because their system of government did not allow colony of the British kingdom to have a representative that had exact power in the parliament. As shown in the poster of “The Able Doctor” the results of such negligence resulted in the Bostonians being oppressed by the wretched hand of the British. After many repeated attempts to talk, to parley, Boston was forced to throw over the British teas over the harbor, thus final streaking and sparking the great fire of revolution. With Patrick Henry powerful speech giving the now tyrannized and abused colonies a reason to fight, to die fighting for their own freedom of instead of living on their knees to the will and binds of the British...
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...The majority of people who watch TV are familiar with the commercials by UNICEF that depict scenes of childhood hunger. The vast majority of these commercials pinpoint countries in Africa that are the most in need. These commercials are known for pictures of emaciated children with tears streaking their faces accompanied by depressing lyrical music. While this is an accurate description of what childhood hunger may present itself as, UNICEF does not acknowledge the hunger epidemic that is plaguing children on American soil. While commercials such as this one and countless others create awareness about child hunger abroad, it is on rare occasion that there is any media coverage of the hunger epidemic in the United States. Members of the Grand...
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...Burroughs’ style is full of rhetorical devices such as figurative language, humor, and forthright diction. One device that he uses often is imagery. He portrays the horror of his youth through rich descriptions of everything from the distressed state of a house to certain erratic characters. One example of this is when Augusten first enters the home of the Finches, the chaotic and often manic family who he was forced to live with and adjust to. “The house smelled like wet dog and something else. Fried eggs? And it was such a mess…I saw more fur. There was fur everywhere, streaking across the carpet, gathered in thick balls in the corners against the wall,” (page 41). He also goes on to describe two of the Finch daughters by saying, “They both had long, greasy,...
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...sometimes there isn’t a single solitary thing you can do about it, except believe with all your heart and soul that there is always a light at the end of the tunnel, no matter how long and dark the tunnel seems right now. However, for thirteen year old Cassandra, believing in that light seemed next to impossible as she stood with the rest of her family, staring at what once used to be her home, before it was destroyed by a freak tornado that hit her home town of Fort Myers just a few hours ago. Now standing, watching the mixed emotions of sadness and grief spread across the pale faces of her family members as they stared at a disintegrated pile of rubble that once was their home, Cassandra saw a single ray of murky sunlight break itself free from the interlocking hands of clouds, and rest silently on a small object on top of the rubble....
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...Microbiology lab study guide Exam 1 Know step by step instructions on use of microscope to view a specimen (image remains in focus even when changing objective lens – parfocal) * Bright field- Dark image against bright background. * Dark field- Field surrounding the specimen appears black while object is brightly illuminated. * Phase contrast- Specimens appear as various levels of “darks” against a bright background. * Fluorescence- fluorescence dye is used which emits fluorescence under UV light.. * Total Magnification = Magnification by the objective lens X Magnification by ocular lens. * FOCUS MICROSCOPE PROPERLY by starting with scanning lens and moving up each objective, using scope’s parfocal property Know step by step instructions for ocular micrometer calibration using the stage micrometer * Calibration = Stage Micrometer /Ocular Micrometer * Stage Micrometer: mm convert to um * Ocular Micrometer: 0 – 100 Ocular Units * EX: 0.2mm X 1,000 um = 200 um * 1mm * Placed stage micrometer superimposed by the ocular micrometer. * First left line of the ocular micrometer aligned with one of the marks on the stage micrometer * EX 25 of ocular micrometer on 8 major lines of the stage micrometer are perfectly aligned= 800 µm ÷ 25 ocular units = 32 um/OU * Record more than one measurement * Calibrate each magnification * Know the different parts of the microscope...
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