...developed to provide nurses with an accurate and systematic method of easily interpreting arterial blood gases. The “6 Easy Steps to ABG Analysis” are listed below for easy reference, and will be explained in more detail in the sections that follow. Lastly, examples will be presented with a systematic review of pertinent findings. The 6 Easy Steps to ABG Analysis: 1. Is the pH normal? 2. Is the CO2 normal? 3. Is the HCO3 normal? 4. Match the CO2 or the HCO3 with the pH 5. Does the CO2 or the HCO3 go the opposite direction of the pH? 6. Are the pO2 and the O2 saturation normal? In order for our analysis to be effective, notes will have to be written next to the results on our lab slip. Alternately, the ABG results can be transcribed onto another paper for analysis (see example one below for the format). www.Ed4Nurses.com 1 6 Easy Steps to ABG Analysis ©2003-2009 Ed4Nurses, Inc. Step 1: Analyze the pH The first step in analyzing ABGs is to look at the pH. Normal blood pH is 7.4, plus or minus 0.05, forming the range 7.35 to 7.45. If blood pH falls below 7.35 it is acidic. If blood pH rises above 7.45, it is alkalotic. If it falls into the normal range, label what side of 7.4 it falls on. Lower than 7.4 is...
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...internal pH in order to survive – even in the event of environmental changes. Many naturally occurring biological, geological, and man-made chemicals are capable or stabilizing the environment’s pH. This may allow organisms to better survive in diverse environments found throughout the earth. In this investigation, you will test a number of substances using pH paper and a pH electrode to determine pH values of household substances. You will also examine the difference between qualitative and quantitative data. Objectives In this experiment you will: • Add an acid to a material and note the changes in pH • Add a base to a material and note the changes in pH • Work with classmates to compare the ability of different materials to test pH [pic] Figure 1 Materials Computer Vernier computer interface LoggerPro Vernier pH sensor Paper towel Wash bottle with distilled water Goggles Beaker 24-well plate pH paper Procedure Part 1: pH tests using pH paper 1. Obtain and wear goggles. 2. Notice the 24-well plate is labeled with A-D for the rows and 1-6 for the columns. 3. Have ONE group member go to the back lab bench and fill the wells halfway with the various household substances. 4. Take a piece of the pH paper and touch it to the substance in well A1. 5. Observe the reaction and record the results in the data table provided. 6. Compare the color of the pH strip to the key on the side of the pH container...
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...The effect of pH on salivary amylase Introduction: Saliva in the mouth contains the protein salivary amylase which acts on starches to break them down into mono- and disaccharides.(2) Saliva has been found to have an average pH level of about 6.78 +/- when tested in various locations of the mouth from multiple healthy individuals.(1) Saliva acts as a buffer against any possible changes in the pH from acids produced from bacteria, maintaining the oral cavity at an almost neutral pH level.(3) If saliva was introduced to a differing pH than its optimal pH, it would cause the amylase action to either decrease or cease functioning all together if the pH changed dramatically enough. With the given information, it could be said that salivary amylase would be at its optimal functioning level at around the average saliva pH of 6.78.(1) A change in pH would be detrimental to amylase given the protein nature of amylase and the instability of proteins in more acidic or basic pH concentrations.(3) When proteins are surrounded by an acidic pH, they become unstable due to the increase in positive charge from the disassociated hydrogen ions, the positive charge acting to pull the proteins out of their folded shape.(3) In similar opposition, when proteins are surrounded by a basic pH, they are instead pulled apart by the negative charge produced by the increased amount of hydroxide ions.(3) Methods: In this experiment, a saliva solution was procured by first...
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...Accurate Indicator for pH Tammy Daniel BIOL2101 9/28/12 Ms. Hunter INTRODUCTION The pH scale is based on the number of hydrogen ions in a solution. The actual concentration of hydrogen ions, [H+], and hydroxyl ions [OH] in moles per liter are indicated for each pH value noted. Acidity and alkalinity can be demonstrated on the pH scale, which ranges from zero to fourteen. Anything less than seven is acidic and anything greater than seven is basic. Seven is neutral with an even concentration of H+ and HO- ions. The farther from the center of the scale a substance is the stronger of an acid or base it is. For example an acid with a pH of one or two is a very strong acid, while a six is a weak acid. As well, a base of thirteen or fourteen is a very much stronger base than that of a pH of eight. The measurements on the pH scale are logarithmic, with each unit representing tenfold change in H+ ion concentration. The idea of the pH scale was devised by a Danish biochemist and part-time beer brewer named Soren Sorensen in 1909. He was searching for a convenient means of checking the acidity of his alcoholic product to prevent its spoilage by bacterial action. (Human Anatomy and Physiology; Seventh Edition ) Acids are proton donors while bases are proton acceptors. Referring to the pH scale numbers zero to fourteen, stomach acid and lemon juice are examples of strong acids having a pH of one and two while ammonia is an example of a base having a pH of 12. Milk and water...
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...Red Onion Extract as pH/ Acid-Base Indicator _______________ A Science Investigatory Project Presented to Mrs. Shellah Marie Tabayoyong-Cruz Marist School, Marikina City _______________ In Partial Fulfillment of the Requirements for the Course Science & Technology 9 _______________ Presented by TAGAÑA, Kurt Lance C. CORDOVA, Chedric Joshua L. CONCEPCION, Ludwig Michael T. 9-St. Titus March 2014 ACKNOWLEDGEMENT The researchers of this study would like to thank Mrs. Gay Fan for sharing her advices and ideas about chemistry. Truly, without her help, this study wouldn’t be made. They would also want to acknowledge the help of Mrs. Aurora Ramos by letting them borrow a pH wheel with pH papers in it. Last, but not the least, they would want to thank Mrs. Shellah Marie Cruz for her efforts in checking, criticizing, and helping the researchers for the good of their study. TABLE OF CONTENTS Abstract 3 Chapter I: Introduction 4 Chapter II: Review of Related Literature 7 Chapter III: Methodology 9 Chapter IV: Presentation, Analysis and Interpretation 11 Chapter V: Generalization 12 Chapter VI: Recommendation 13 Bibliography 14 ABSTRACT The study aims to provide chemists a source of an accurate acid-base/ pH indicator which is abundant and can be found anywhere in order to help them in certain laboratory situations such as identifying whether a solution is an acid or base. Red onion...
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...Determining the equilibrium constant from pH values of changing concentrations of ethanoic acid Design Research question: How will altering the concentrations of ethanoic acid affect the pH value, and, in-turn, the equilibrium constant? Background information: When weak acids react, the reaction typically does not go to completion. Rather, the system goes to an intermediate state in which the rates of the forward and reverse reactions are equal. Such a system is said to be in chemical equilibrium. When equilibrium is reached, the reactants and the products have concentrations which do not change with time. When in equilibrium at a particular temperature, a reaction mixture obeys the Law of Chemical Equilibrium, which imposes a condition on the concentrations of reactants and products. This condition is expressed in the equilibrium constant Kc for the reaction. In this experiment, I will study the equilibrium properties of the reaction between ethanoic acid, otherwise known as acetic acid (CH3COOH) and water (H2O): CH3COOH(aq) + H2O(l) CH3COO-(aq) + H3O+(aq) When solutions containing ethanoic acid and water are mixed, they react to some extent, forming CH3COO- and H3O+. As a result of the reaction, the equilibrium amounts of CH3COOH and H2O will be less; for every mole of CH3COO- formed, one mole of CH3COOH and one mole of H2O will react. The equilibrium constant expression Kc for Reaction 1 is: Kc = [CH3COOH] / [H3O+][CH3COO-] The value of...
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...Effects of Different pH Level on Amylase in Starch Introduction Amylase, a family of proteins that differ in isoforms, is a digestive enzyme found in saliva and pancreatic fluid that helps digest starch into simple sugars. (Scannapieco et. al., 1993). Amylase is the first step in digesting starch, which is used for the intake of carbohydrates or energy in humans. (Butterworth et. al., 2011). Amylase functions in the hydrolysis of starches, which produces glucose monomers. (Karp, 2010). This is essential in glycolysis and the harvesting of ATP. Since amylase is also a protein that performs enzymatic reactions, the secondary and tertiary structures are affected by external stimuli like pH. (Karp, 2010). When the structure is altered the affinity of the enzyme is changed. (Luesse, 2012). The point where the majority of enzymes are found to be most active is in the neutral pH range near 7. (Guyot et. al., 2000). Therefore, it can be said that extremely high or low pH’s denature an enzyme. By finding the specific pH at which amylase is most active, optimum production can be achieved. (Guyot et. al, 2000). In our experiment, we want to determine what pH levels is optimum for the enzymatic activity of amylase. To see if starch is broken down to glucose amylase, a spectrophotomer is used to see the amount of light that passed through. The more light that passes through the test tube means more starch was broken down into glucose. (Luesse, 2012). We hypothesized that the enzyme...
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...Rose Petal pH Indicator Strip II – Proponents Jhed Angelo Nemuel Parcon Princess Hope Delos Reyes Jalel Pasbal Maureen Suganob Nel Joshua Turaray Laureen Suganob Reed Elcen Lumbay II – Objective This project aims to produce a pH indicator strip made from rose petals. The researchers want know if the rose flower is an effective acid-base indicator and if it would be precise when used to identify the pH levels of household items. III – Introduction Normally, pH indicator strips such as litmus paper are the ones used in indicating the pH level of a substance. pH is the measure of the acidity or alkalinity of a solution, numerically equal to 7 for neutral solutions, increasing with increasing alkalinity and decreasing with increasing acidity. Acidic and basic are two extremes that defines chemicals. The pH scale measures how acidic or basic a substance is. It ranges from 0 to 14. A pH of 7 is neutral. A pH less than 7 is acidic, and a pH greater than 7 is basic. Each whole pH value below 7 is ten times more acidic than the next higher value. For example, a pH of 4 is ten times more acidic than a pH of 5 and 100 times (10 times 10) more acidic than a pH of 6. The same holds true for pH values above 7, each of which is ten times more alkaline—another way to say basic—than the next lower whole value. For example, a pH of 10 is ten times more alkaline than a pH of 9. (http://www.epa.gov) Roses are woody perennial shrubs or vines of the genus Rosa, within the family...
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...polypeptide chains that make up their structure (Reece, 2011). The functions of proteins are dependent on their structure, and since enzymes are almost always proteins, the structure of the enzyme is very important for the enzyme to function. However, there are factors that can disrupt the structures of enzymes. These can be environmental factors such as temperature and pH, or they can be concentration changes, such as an increase or decrease in enzyme or substrate concentration (Eed, 2013). Temperature is an environmental factor that can alter enzyme activity (Reece, 2011). An increase in the kinetic energy of a solution results in an increase in temperature (Reece, 2011). As the temperature increases, the molecules in the solution move faster, resulting in an increased number of collisions between enzymes and substrates. This will speed up the rate of reaction because enzymes and substrates bind together. But if the temperature is too high, this can denature, or disrupt the structure, of the enzyme (Eed, 2013). pH is another environmental factor that can effect enzyme activity (Reece, 2011). A pH too high or...
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...6 Potentiometric measurement of pH Theory: see Electrochemical cells and electrodes, p. 6-29 Potentiometric measurement of pH using hydrogen electrode, p. 7-36 Potentiometric measurement of pH using glass electrode, p. 8-41, in Kopecký F., Kaclík P., Fazekaš T.: Laboratory manual for physical chemistry, Department of Physical Chemistry, FaF UK, Bratislava, 1996 Task: Measure pH of 7 different aqueous solutions using: A: hydrogen electrode B: glass electrode. C: quinhydrone electrode Equipment and chemicals: Chemicals: 7 aqueous solutions with different pH, distilled water Equipment for task A: The potentiometer for the measurement of the electromotoric force, cell with the hydrogen and calomel electrodes, hydrogen gas in the steel cylinder with valve and manometers, thermometer, beakers, filtering paper An electrochemical cell (Fig. 7-1, p. 7-38) includes: hydrogen electrode with the platinized platinum (1) placed inside of tube connected with the hydrogen gass valve (1). Saturated calomel electrode (3) is used as the reference electrode. Electrodes are immersed in the measured solution (2). For storage, hydrogen electrode should be kept in distilled water, and the calomel electrode in the saturated KCl solution. It is necessary to prevent the sensitive parts of electrodes from drying out. Procedure: Manipulation with the hydrogen gas valve is allowed to the instructor only, or under his supervision! 1. Take off electrodes from the storage solution...
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...Temperature, pH, Enzyme Concentration , and Substrate Concentration on Catecholase Introduction Enzymes are biological proteins that speed up the reaction rate of a chemical reaction. They work in the human body by lowering activation energy making certain that reactions will initiate. For every action, there is an equal and opposite reaction. In this case, factors that influence the activity of an enzyme are called modulators. If modulators activate enzymes the reaction rate catalyzed will significantly increase, but if the modulator inactivates enzymes the reaction rate catalyzed will significantly decreased (Silverthorn, 2004). The potentially disastrous influence of temperature, pH, enzyme concentration, and substrate concentration on enzymes and other proteins is one reason why these modulators are very strictly regulated by the body (Silverthorn, 2004). Temperature, a measure of the intensity of heat, is an important factor in the activity of enzymes. The velocity of an enzymatic reaction is influenced by temperature. This is because substrates collide with active sites frequently in the presence of rapidly moving molecules. In addition, although these molecules do move rapidly the speed of the reaction drops sharply. In short, thermal agitation causes protein molecules (enzymes) to denature ( breakdown of protein structures). All enzymes have an optimal temperature at which reaction rates go fastest without denaturing the enzyme (Campbell and Reece, 2002) pH, a measure...
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...SHRIPAD COMPUTERISED CLINICAL LAB. Add- Dr.Harne Hospital. Shriramwadi GHOTI. Ph.9822215939/ 8793350162 Mrs.Pragati R.Harne. Time- 9 am-9pm. (B.Sc.D.M.L. Patient's Name Mr.Bhaskarrao Shirsath. Date- 10/5/13 Reffered By Dr. R.P.Harne.(DHMS) HAEMATOLOGY Haemoglobin (Hb) - 13.9 gm%(N.R.-11-15gm%) White Blood Cells - Total Count - 2,700 mm(N.R. - 4000-10,500cumm) Deffential Count Neutrophils - 69 % N.R. - 45-65 %) Lymphocytes - 24 % N.R. - 25-45 % Eosinophils - 06 % N.R. - 1-6%) Monocytes - 01 % (N.R. - 4-8 %) Basophils - 00 % (N.R. - 0-1 %) Red Blood Cells - Pletelet Count - 1.23 Lakh /cumm (N.R- 1.5-4.5 Lakh) ESR - mm/hr (N.R.- 0 - 20 mm/hr) Blood Group - Rh Factor - Bleeding Time (BT) - . (N.R. - 1-3 min.) Clotting Time (CT) - (N.R. - 3-5 min.) Mrs.Pragati R.Harne. SHRIPAD COPUTERISED CLIN ICAL LAB. Add- Dr.Harne Hospital. Shriramwadi GHOTI. Ph.9822215939/8793350162 Mrs.Pragati R.Harne. Time- 9 am-9pm. (B.Sc.D.M.L.T.) Patient's Name- Mrs.Taibai Chavan. Date- 6/5/13 Reffered By Dr.- R.P.Harne.(DHMS) HIV I & HIV II ANTIBODY TEST RESULT - HIV I - Non Reactive. HIV II - Non Reactive. METHOD - Enzyme Immune Assay (EIA) for the detection...
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...Ateneo de Naga University High School Km. 7, John Phelan Drive, Pacol, Naga City The Comparative Analysis of the pH Level and Lactose Content of Fresh Milks and Processed Powdered Milks Jenna Joyce Ebona Glowsy Cabral Nicole de Vera Rogelenne An Borja Renz John Alba Johannah Aguayo RESEARCHERS Mrs. Ma. Wendy Solomo ADVISER ACKNOWLEDGEMENT This study aimed to compare the pH level and Lactose content of fresh milks (Cow’s milk, Goat’s milk, and Carabao’s milk) and processed powdered milks (Bear Brand, Nido, and Alaska) in order to guide the consumers in choosing what kind of milk to buy. Nevertheless, this study would not be possible without the everlasting love, care, and assistance from the researcher’s family, who supported in the collection of milk samples for the experiment and encouraged the researchers when they were taken aback by discouragement. The researchers would never forget to acknowledge Mrs. Ma. Wendy Solomo who extended, in her precious time, patience in explaining the whole course of the research and gave words of encouragement and for the continuous help, guidance, and assistance extended to the researchers. Furthermore, the researchers would like to thank their friends especially their classmates for the shared information and for helping the researchers whenever they need help. The researchers would alsow like to thank the staffs at the INECAR department...
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...Biology Lab Fall 2014 Determining the properties of an enzyme Abstract: To determine the properties of an enzyme, a peroxidase, turnip extract is used in this experiment Enzymes are large proteins that are responsible for the speed at which chemical reactions they are involved in taking place. Enzymes speed up chemical reactions by lowering the activation energy of a reaction, the amount of energy necessary to trigger a reaction .Using Peroxidase ,Turnip extract which is expermitnatal easy to prepare and examime that is This experiment determines the effects that concentration temperature, ph, boiling have on an enzymes ability to perform its work. It is hypothesized that none of these variables will have any effect on the activity of the enzyme. Introduction : This expermient with present various temperates and ph levels to be teste in order to determine the properties of an enzyme. An enzyme is a protein that acts as a catalyst, that changes the rate of a reaction with no help of energy. Enzymes lower the activation energy of a reaction the amount of energy necessary to trigger a reaction. Most enzymes are proteins that have a unique shapes which are determined by their amino acid sequences.The shape of the enzyme called the active site determines its catalytic effects. The active site of each type of a unique shape that allows the enzyme to bind with only certain kinds of enzymes called the substrate. Smaller molecules are called...
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...follows. C1, C2(These will be the control variables) 1,2,3,4,5(these will be the experimental variables). Step3: Once all the test tubes are labeled with the correct number place each one in the test tube rack in in order from C1 to C2 on one side of the rack and test tubes 1 through 5 on the other side of the rack. Step 4: Using the graduated cylinder, measure out 10mL of the water with a pH of 7. Pour the water into one of the test tubes. Repeat this step until all 7 of the test...
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