...In biology, in order to purify biological molecules, a common process called chromatography is often used. This process involves the separation of the large particles in the solution from the smaller particles. This is instrumental in filtering the solution, but without specialty scientific instruments, there is no way to immediately know the concentration of the, now separated, solution. However, there are multiple processes that are utilized to find unknown concentrations of a solution. One of these, known as the Bradford method, allows for an accurate, fairly simple, and timely assessment of these unknown concentrations by using a dye called the Coomassie Brilliant Blue G-250. The samples that contain this dye are run through a spectrophotometer,...
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...Can the Brilliant Jerk Be Managed Effectively? By James Haskett JAMES HESKETT James Heskett is a Baker Foundation Professor, Emeritus, at Harvard Business School. ORIGINAL ARTICLE The annoying employee who makes his numbers while alienating those around him will gain needed attention in the coming months with at least one book about to be published on the subject. This is an age-old problem that most managers handle badly. You know the story by now. It concerns high-performing employees, known by some as "stars" and by others as "destructive heroes" or "brilliant jerks," those who generate a great deal of business while creating problems for colleagues. They are demanding to the point of being abusive, they make promises to clients that their colleagues cannot meet, they take too much credit for success, and they generally are unable to adhere to commonly shared values of members of the organization. The management response to this kind of situation is too often ineffective. By their own admission, their managers are reluctant to rock the boat as long as the numbers continue to be good. In doing so, they underestimate the costs to the organization, including the loss of other talent. And when they do act, they do so much too slowly, often after most of the damage has been done. Jack Welch has written about the phenomenon of what he calls "jerks" or "bullies" from his own experience. At GE they were referred to as a "Type 4" manager, "the person who delivers on all...
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...that they could be proud of. So, I explored and put my knowledge to the test to come up with an experimental study of comparing mayonnaise-sprite solution and certain product and determine which one could show satisfaction among woman. Then, I simply conducted a comparative study of Cream Silk and Mayonnaise-Sprite solution. Conclusion: Therefore I conclude Mayonnaise-Sprite solution is better hair conditioner than cream silk because it is made out of eggs and causes fair to produce natural keratin. It gives us a brilliant-looking hair that is bouncy, full of life, smooth and healthy hair. Title: A comparative study between mayonnaise-Sprite solution and Cream Silk as better conditioner. MAYONNAISE TABLESPOON CREAM SILK SPRITE Problem: Can mayonnaise-Sprite solution be better hair conditioner than cream silk? Hypothesis: Mayonnaise-Sprite solution can be better hair conditioner than a commercial conditioner called Cream Silk. PROCEDURE: 1. Prepare and secure all materials needed. 2. Put the...
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...Can the Brilliant Jerk Be Managed Effectively? By James Haskett JAMES HESKETT James Heskett is a Baker Foundation Professor, Emeritus, at Harvard Business School. ORIGINAL ARTICLE The annoying employee who makes his numbers while alienating those around him will gain needed attention in the coming months with at least one book about to be published on the subject. This is an age-old problem that most managers handle badly. You know the story by now. It concerns high-performing employees, known by some as "stars" and by others as "destructive heroes" or "brilliant jerks," those who generate a great deal of business while creating problems for colleagues. They are demanding to the point of being abusive, they make promises to clients that their colleagues cannot meet, they take too much credit for success, and they generally are unable to adhere to commonly shared values of members of the organization. The management response to this kind of situation is too often ineffective. By their own admission, their managers are reluctant to rock the boat as long as the numbers continue to be good. In doing so, they underestimate the costs to the organization, including the loss of other talent. And when they do act, they do so much too slowly, often after most of the damage has been done. Jack Welch has written about the phenomenon of what he calls "jerks" or "bullies" from his own experience. At GE they were referred to as a "Type 4" manager, "the person who delivers on all...
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...Can the Brilliant Jerk Be Managed Effectively? By James Haskett JAMES HESKETT James Heskett is a Baker Foundation Professor, Emeritus, at Harvard Business School. ORIGINAL ARTICLE The annoying employee who makes his numbers while alienating those around him will gain needed attention in the coming months with at least one book about to be published on the subject. This is an age-old problem that most managers handle badly. You know the story by now. It concerns high-performing employees, known by some as "stars" and by others as "destructive heroes" or "brilliant jerks," those who generate a great deal of business while creating problems for colleagues. They are demanding to the point of being abusive, they make promises to clients that their colleagues cannot meet, they take too much credit for success, and they generally are unable to adhere to commonly shared values of members of the organization. The management response to this kind of situation is too often ineffective. By their own admission, their managers are reluctant to rock the boat as long as the numbers continue to be good. In doing so, they underestimate the costs to the organization, including the loss of other talent. And when they do act, they do so much too slowly, often after most of the damage has been done. Jack Welch has written about the phenomenon of what he calls "jerks" or "bullies" from his own experience. At GE they were referred to as a "Type 4" manager, "the person who delivers on all...
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...Experimental Procedure Materials and Reagents The following materials and reagents were used throughout the experiment: P200 and P100 Pipetman micropipettes were used in this experiment. Fisher gel loading pipet tips were used. A Genesys 10S UV-VIS Spectrophotometer was used and had the ID number 2N3R294201. Two cuvettes (1.0cm path length) were used. A black Sharpie marker was used. A Fisher vortex mixer was used and had the ID number 060711069. A Fisher Scientific Isotemp was used and had the ID number 102NO562. Coomassie Brilliant Blue dye stain (2.5 g dye, 50% methanol, and 7.0% Acetic Acid in water) made on 4/22/2015 was used. Gel de-staining solution (50% Methanol/7.0% Acetic acid) made on 10/16/2013 was used. Two magnetic...
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...The purpose of this experiment is to find the unknown concentration of two different proteins; the gamma globulin, and the bovine serum albumin. The measurement is done in the spectrophotometer by using the method of the Bradford Essay with the Coomassie Brilliant Blue G-250 dye, as a reagent. To identify the unknowns, first a colorimetric assay of both proteins with known concentrations is executed, and the data in plotted in two standard curves respectively. Finally, the unknown absorbance measurements are compared with the standard curves to find the absolute concentration, which is the most accurate measurement. Introduction Life as we know it is possible for the presence of the same elements repeatedly distributed throughout the matter that surrounds us in nature. These elements form molecules that in turn are the building blocks of every biological cell. Significantly, the information obtained from nature to understand life and evolution are the product of an applied scientific method, which has been improved by the invention and use of technology over time. Nowadays, Scientific are able to measure the components of specific samples precisely, but there are cases when limitations are present in their investigations. For example, that is the case of the measurement of proteins, which is not as easy as looking a sample through a microscope to be able to count how many proteins are present in the biological sample. Proteins are an...
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...The Brad assay * is one of the most common forms of measuring absorbance through a spectrometer * uses a a dye (coomassie Brilliant Blue G-250) that binds to proteins and is absorbed at a wavelength of 465- 598 nm * Fast, therefore can meaure a 96 plate in 5 minutes * Absorbance shift allows the measure the protein at the 598 nm wavelength by using the spectrometer, can be justified through the beer-lambert law * The standard curve can be used to estimate the concentration of an unknown protein in solution by * Taking in account their known absorbance levels * Use line equation from curve * The unknown protein conc. in a sample with a known absorbance level can be determined Bradford Vs. Lowry * Lowry * Uses alkaline conditions- reduction cu 2+ to Cu+ * Folin phenol reagent- yellow to blue * Peptide bonds for monocovalent copper and radicals of try and trp react with folin * Concentrations 0.1-1.0 ug/ml of protein * Can be interfered with easily * Slow 40-60 * Colour varies with proteins * Criticial timing with procedure * * Bradford * Based on absorbance shift from brilliant blue when bound to proteins * 465- 598 (when bound) * Acidic conditions * Binds tightly to protein, and inhibits binding sites (little inference) * Limitations * Strong basic buffers Detergents interfere with binding of the cosmic blue, causing...
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...Comparison – Contrast Essay in Meyer’s The Seven-Per-Cent Solution Father of detective work, Sherlock Holmes and father of psychoanalysis, Sigmund Freud, can be compared and contrasted in Nicholas Meyer’s The Seven-Per-Cent Solution. Sherlock Holmes can be identified as one of the most brilliant fictional characters of the 19th century period. As a “consulting detective”, he uses his extraordinary abilities and logical reasoning to solve some of the most difficult cases. Equally as brilliant in his field, Sigmund Freud is identified in the novel as a neurologist that extensively studies the human mind and behavior. Both Sherlock Holmes and Sigmund Freud share many similarities in Meyer’s novel. One obvious parallel is each character’s remarkable abilities in their profession. In chapter IV Holmes does an impressive analysis of the Dr. Freud and surprises everyone after using clues to identify many of the Dr.’s personal and professional characteristics. Freud’s intelligence is likewise showcased when he is able to reveal the underlying cause of Holmes’ addiction to cocaine in the last paragraph of the story. Another similarity is that both characters are loners. Other than Dr. Watson, Holmes does not trust or allow others in his life. As for Dr. Freud, he is excluded from society and colleagues due to his radical ideas and methods of work. Both characters also happen to smoke Tobacco. For Holmes it was “huge amounts of shag”, during his cases whereas Freud would...
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...Making Hotplates Tony Bennett Ashford University BUS644: Organizational Behavior/William Adams March 10, 2013 The summary will examine a company consisting of a group of workers who are responsible for assembling hotplates which are used in hospitals and medical laboratories to bring solutions to certain temperatures. A job of this nature requires a certain amount of patience and skills that some people could find hard or impossible to handle. The goal of the summary is to answer specific questions about the environment in which they worked, the managers and what did or did not motivate the employees. This analysis is done to obtain a better understanding of how motivation affects different aspects of working environments. Increasing Productivity What changes in the work situation might account for the increase in productivity and the decrease in controllable rejects? The increase in productivity and the decrease in controllable rejects are contributed to one decision, the introduction of the individual workstation. Separating the employees increased morale and made them accountable for their own work. The new assembly set up was perfect for everyone, the managers and also the company. Increasing Moral What might account for the drop in absenteeism and the increase in moral? Based on the information provided it appears the employees did not get along well. There are many factors that contribute...
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...future technology. Robots can transcend any actual ability of human. https://www.ted.com/talks/jinha_lee_a_tool_that_lets_you_touch_pixels This is incredible idea and work. Video gives the information about to robotic technology as the world is growing speedily to the robotic technology. After watching video one can feel the powerful future technology. His presentation was very knowledgeable. He delivers the great idea regarding future technology. In short his presentation is really inspiring for the teenagers. https://www.ted.com/talks/shyam_sankar_the_rise_of_human_computer_cooperation Shyam saknar in his presentation said that the human brain is more powerful than any computer device. By utilizing human brain one can makes the solution of any problems in technology. As the world has become global village technology is growing speedily day by day just because of human brain. Technology needs more research, methods and experiments which can only give by human brain. His presentation is totally inspiring. https://www.ted.com/talks/daphne_koller_what_we_re_learning_from_online_education Now a days...
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...bringing meaningful innovation to our customers. And the best inspiration for innovation comes from none other than our customers.” The much awaited device offers the flagship experience of its predecessor, the Galaxy S5, in a compact design. With a host of innovative features like water & dust resistance, ultra power saving mode and heart rate monitoring, the Galaxy S5 Mini delivers a powerful performance that truly enhances your lives. Equipped with Android 4.4 Kit Kat OS, the Galaxy S5 Mini comes with a spectacular 11.3 cm (4.5”) HD Super AMOLED screen which delivers brilliant images that come to life, while still staying super slim and compact. Powered by a Quad Core 1.4GHz processor along with 1.5GB RAM and 16GB ROM, S5 Mini delivers a turbo-charged experience. A micro SD card slot also stands ready to add up to another 64GB, should you need more memory space. Galaxy S5 Mini also comes equipped with a brilliant 8MP rear-facing camera and a 2.1MP front camera that allows you to capture everyday events and turn them into special moments. “The Galaxy S5 Mini is yet another manifestation of the philosophy of people inspired innovation that drives Samsung products. This stylish smartphone is the perfect combination of performance and power in a conveniently compact design. Effortlessly smart...
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...mayonnaise-sprite solution and certain product and determine which one could show satisfaction among woman. Then, I simply conducted a comparative study of Cream Silk and Mayonnaise-Sprite solution. Title: A comparative study between mayonnaise-Sprite solution and Cream Silk as better conditioner. Problem: Can mayonnaise-Sprite solution be better hair conditioner than cream silk? Hypothesis: Mayonnaise-Sprite solution can be better hair conditioner than a commercial conditioner called Cream Silk. Background of the Study: As our generation advances to move forward. Women are having a great passion and are craving to have spontaneous-looking hair. And so, I compared my very own Mayonnaise- Sprite solution and Cream Silk to which one would take a better reaction to your hair as better Performing hair conditioner. As I researched more in the field of study about hair, a revolutionary breakthrough hit me. I just found the our hair is composed of natural keratin. And as we grow older our hair loses some keratin due to too much shampoo or too much frequent brushing. That’s why we have dull, frizzy and unmanageable hair. And since mayonnaise is made out of real eggs, protein is present in the substance. And as we are prone to eat food or apply products that are rich in protein, it is easily conduced into keratin. Some of it are turned into natural keratin and some if it are being used u keratin is naturally present in our solution. I made this product to help women achieve brilliant-looking...
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...Failure is the lack of succeeding, but success is an accomplishment of an aim or a purpose. However, sometimes success happens unwillingly or unexpected. These are what are called happy accidents. Some of the most popular products invented today were not even planned; they just happened. Everything may not go as intended, but the outcome can be rewarding. There is a fine line between a brilliant invention and absolute failure. Some of the best inventors had discovered this theory. As exploration of thinking outside the box expands, good things can start to accumulate and invent something useful. The feeling of these happy accidents can feel good once they are accomplished. The feeling of self worth takes over and confidence boosts. When the...
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...Study Between Mayonnaise – Sprite Solution And Cream Silk As Better Hair Conditioner Problem: Can Mayonnaise – Sprite solution make our hair shinier than Cream Silk? How long does Mayonnaise – Sprite solution makes our hair shiny? Hypothesis: Mayonnaise – Sprite solution can make our hair shinier than Cream Silk. It can make our hair shiny for a whole day. BACKGROUND OF THE STUDY As our generation advances to move forward women are having a great passion and are craving to have a spontaneous-looking hair. And so, we compared our very own Mayonnaise-Sprite solution and Cream Silk to see which one would take a better reaction to your hair as better performing hair conditioner. As we researched more in the field of study about hair, a revolutionary breakthrough hit us. We just found that our hair is composed of natural keratin. And as we grow older, our hair loses some keratin due to too much shampoo or too much frequent brushing. That’s why we have dull, frrizzy and unmanageable hair.And since Mayonnaise is made of real eggs, protein is present in the substance. And as we are prone to eat foods or apply products that are reach in protein, it is easily conduced into keratin, Some of it are turned into natural keratin and some of it are being used up keratin is naturally present in our solution. We made this product to help women achieve brilliant-looking hair that is bouncy,full of life...
Words: 455 - Pages: 2