...Introduction In race carbohydrate is a key aspect to endurance performance, especially when the events lasts in extent of 1-2 hours. It is common practice within the endurance community to utilize carbohydrates as the substrate, as glycogen depletion is seen as a key mechanism in performance outcome[2,4, 14]. Providing exogenous carbohydrates into the body, in theory, spares endogenous carbohydrate reserves allowing for increased exercise time[5]. The current recommendation from the American College of Sports Medicine is feeding at regular short intervals (15-20 min), totaling 30-60 g/hour of a mixture of carbohydrates (glucose, fructose, maltodextrin), when the events exceeds 1 hour[8]. However, more recent reviews have questioned this...
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...London School of Engineering and Materials Science Laboratory report writing instructions DEN101 - Fluid Mechanics 1 Flow Rate Measurement Experiment A. Student Student Number: 1234567 Version 2.0, 27 November 2010 Template for Word 97-2003 Abstract This document explains what is expected in your Fluids 1 lab report. The sections that should be covered are outlined and a structure you could follow is proposed. Detailed advice on how to edit the report is given. The document concludes with the marking criteria for this lab report. Table of Contents Abstract 2 1. Introduction 3 1.1. Writing 3 1.2. Editing and formatting 3 1.3. Content of the introduction 4 2. Background and theory 4 3. Apparatus 4 4. Test 4 5. Experimental procedure 4 6. Results 5 7. Discussion 5 8. Conclusions 5 9. References 5 10. Appendix A: Marking criteria 6 Introduction Before starting to write a report, you should think about what is your audience. Am I writing for colleagues who want a lot of detail how it is done, or am I writing for my boss who just wants an executive summary as he has no time for details? In general, there is not a single type of audience and we have to make our writing suitable for the detailed read, as well as the fast perusal. To understand what is required from you in this report, please have a look at the marking criteria in the Appendix. 1 Writing To limit...
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...HCl in Two Carbohydrates Solutions.). Please ensure the correct file(s) is (are) uploaded before clicking 'Finish'. Marks will be deducted if instructions in the lab manual and below are not adhered to. By submitting via this link, you acknowledge that you will adhere to all the instructions herein. * Reports are to be submitted in pairs (2 persons per group, both have to submit the SAME report). * Font: Arial, Size: 11, single-spaced. Step 1: Create a WORD file as, ‘Name_Partner's name_Practical group_No.of practical_Title.doc' e.g: Shalini_Prem_PG1_P3_Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions. The following MUST appear on the first page of Word: • Name: • Partner’s name: • Class: P.... • Date of lab class: (Day), (date)..... • Program: Foundation in Science • Unit code: FHSB 1214 • Unit description: Biology I • Year and trimester of study: 2013, trimester 1 • Title of lab report: Practical 3: Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions. • Lecturer’s name: Step 2: On the 2nd page of your Word file present your tables. Report must include: (1) title (2) objective - NO need to write the procedure and materials. (3) Result - Table 1 & 2, based on ACTUAL observation obtained in class. You may read manual page 7, 8 & 27 on how to write the observation. No need to include expected results in your report. (4) Discussion-answer all question (Question 1-9) in the lab manual (based...
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...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solutions ____________________________________________________________________________________ Objective: Students are expected to state the objective of this experiment. Apparatus & Equipments: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath, ~ 37 oC Water bath, ~ 95oC Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3 M Hydrochloric acid 3 M Sodium hydroxide Procedures: 1. Prepare two boiling tubes containing 1 ml solution A and 1 ml solution B respectively. Add 1 ml Benedict’s solution to each tube. Heat both tubes together in the (~95oC) water bath for two minutes. Record the results in table 1. 2. Add a few drops of fresh solution A and B separately spaced on a white tile. On each solution, add 1-2 drops of iodine solution. Mix with pen cover. Record your observations in the table 1. 3. Pipette 2 ml solution B into each of four boiling tubes. Label the tubes 1, 2, 3 and 4 respectively near mouth of tube. Label your group name. 4. Place tubes 1 and 2 in a water bath of ~37oC. (It doesn’t matter how long you put it in at this stage as no saliva or HCl have been added yet). 5. Salivate into a small beaker till it reaches about 5 ml. 6. Step (6) and (7) is to be done approximately at the same time. Measure out 4 ml of the saliva prepared in step (4) and pipette 2 ml each into tubes...
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...Student Sheet Name: Cheryl R Logsdon Date: September 4, 2015 Instructor’s Name: Christopher Haines Assignment: SCIE207 Phase 2 Lab Report Title: Animal and Plant Cell Structures Instructions: Your lab report will consist of the completed tables. Label each structure of the plant and animal cell with its description and function in the provided tables. When your lab report is complete, submit this document to your instructor in your assignment box. 1. Animal cell: Observe the diagram that shows the components of an animal cell. Using the textbook and virtual library resources, fill in the following table: Animal Cell Number Cell Structure Description and Function 1 Chromatin (DNA) The primary functions are to package DNA into a smaller volume to fit the cell, to enforce DNA macromolecule to allow mitosis, to prevent DNA damage, and to control gene expression and DNA replications 2 Nucleolus The largest structure in the nucleus of eukaryotic cells where it primarily serves as the site of ribosome synthesis and assembly. 3 Nucleus The nucleus is basically the command center of the cell, it controls eating, movement, and reproduction. 4 Nuclear Pore Are large protein complexes that serve as the pathways for exchange of the materials between the nucleus and cytoplasm 5 Nuclear Envelope A highly regulated membrane barrier that separates the nucleus from the cytoplasm in eukaryotic cells. 6 Centrioles Self-replicating organelles made up of nine bundles...
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...1 M hydrochloric acid Sudan III Starch solution Corn oil Egg albumin 1% copper sulphate solution 1% sucrose solution 0.1 M Sodium hydroxide 1% glucose/fructose/lactose solution Absolute ethanol DCPIP (dichlorophenolindophenol) solution Ascorbic acid Introduction The nutrients in the food you eat supply your body with energy for growth and repair. These principle substances include carbohydrates, proteins, fats, minerals and vitamins. We can test for the presence of these important compounds in food by using chemical reagents that react in predictable ways in the presence of these nutrients. Please refer to the notes given above on: How to record qualitative data. (Marks will be awarded based on proper recording.) What to do if you don’t obtain the desired results. Flowchart Students will be allowed to proceed with the experiment only if they have come into the laboratory with a flowchart of the day’s experiment. Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell Biology 14 Procedures: Part 1: Identification of Carbohydrates (A) Test for reducing sugars The reducing sugars include all monosaccharide, such as glucose and fructose, and some disaccharides, such as maltose and lactose, using 0.1 – 1% sugar solutions. Common tests for reducing sugars include Benedict’s test (described below) and Fehling’s test (not described here). See ‘basis of test’ below for explanation of the following reaction: Benedict’s test...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme studies I (Experiment 2) Practical 4 Enzyme studies II Practical 5 Cell studies I - - Practical 10 Energetics II Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell...
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...The purpose is to find which liquid would be the most effective in dissolving blow pops. In the previous year, research has shown that the two different type of substances, polar and nonpolar affects whether something would dissolve. In this lab, we will test Blow-Pops into 4 different liquids, water, oil, soda, and vinegar to see which would be the most effective. First, we put the Blow-Pops into the different cups provided with the different liquids, then, we observe and check after every five minutes to see if the liquids have any effects on the Blow-Pop. Lastly, we concluded that out of the four different liquids we used, vinegar was the liquid that Blow-Pop would dissolve the fastest in. It has long be considered that sugar can...
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...IDENTIFICATION OF UNKNOWN BACTERIA It is virtually impossible to identify bacteria based on physical characteristics alone. This is due to the fact that there are only a few basic shapes and physical features commonly seen in the prokaryotic world. Instead, biochemical testing has been used to make bacterial identification down to the “species” level. These schemes are based on creating and matching biochemical profiles of the production of enzymes, acids and gases by isolated pure cultures of a given microorganism. Identification schemes and flow charts can be found in reference texts such as “Bergey’s Manual of Determinative Bacteriology” or “The Prokaryotes”. Each group of students will receive a TSA slant or broth containing a pure culture of an unknown bacterium belonging to the Family Enterobacteriaceae. It is the responsibility of the group to maintain stock cultures of the organism provided. Working stock cultures will be used to inoculate the various biochemical test media over the next several weeks and should be fresh and free from contaminants. A reserve stock culture should be made and after incubation and comparison with the original slant, kept with the original slant in the refrigerator. It is critically important that aseptic techniques are used during transfers and inoculations to prevent contamination of your cultures. If contamination is suspected, you will be able to fall back to your reserve stock. If you fail to maintain a reserve stock...
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...PRACTICAL REPORT NAME | Dylan Yong Chun Yen | PARTNER’S NAME | Ng Cheau Wen | PRATICAL GROUP | PG 15 | DATE OF PRATICAL SESSION | 18th June 2014 | PROGRAMME | Foundation in Science ( P stream ) | UNIT CODE | FHSB 1214 | UNIT DESCRIPTION | Biology I | YEAR &TRIMESTER OF STUDY | 2014 Trimester 1 | TITLE OF LAB REPORT | Practical 3: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions | LECTURER’S NAME | Ms. Bong Siew Mee | Title: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions Objective: To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate solutions. Results: Table 1: Observations made when two carbohydrate solutions provided in laboratory were tested with Benedict and Iodine solution. | Observations | Conclusions | Solution A | Benedict’s test: An initial blue translucent mixture turned to brick-red opaque solution and moderate amount of precipitate settled after heated at a high temperature for two minute. | Presence of reducing sugar | | Iodine test: The translucent colouration of the mixture retained its yellowish-brown colour. | Absence of starch | Solution B | Benedict’s test: The translucent colouration of the mixture remained its blue colour...
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...Biologically Important Molecules, Carbohydrates, Proteins, lipids and Nucleic Acids Tiffany Osbey Jackson State University Lab Report Authors: Tiffany Osbey Portia Mcmlertrey Jamia Ransome Darrius Bryant Abstract: Organic molecules are those primarily made up of carbon, hydrogen and oxygen. The common organic compounds of living organisms are carbohydrates, proteins, lipids, and nucleic acids. Each of these macromolecules (polymers) are made of smaller subunits (monomers). The bonds between these subunits are formed by dehydration synthesis. This process requires energy; a molecule of water is removed (dehydration) and a covalent bond is formed between the subunits (Fig.1). Breaking this bond is called hydrolysis; it requires the addition of a water molecule and releases energy. Each class of these macromolecules has different structures and properties. For example, lipids (composed of fatty acids) have many C-H bonds and relatively little oxygen, while proteins (composed of amino acids) have amino groups (-NH3+) and carboxyl (-COOH) groups. These characteristic subunits and chemical groups impart different properties to the macromolecules. For example, monosaccharide’s such as glucose are polar and soluble in water, whereas lipids are non-polar and insoluble in water. Objective: Perform lest to detect the presence of biology important carbohydrates, proteins lipids and nucleic acid. Explain the importance of a positive and a negative control in biochemical test. Use biochemical...
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...Food Chemistry Laboratory report The analysis of Lipids Determination of fat by Soxhlet Extraction and Determination of fat content in milk by Gerber method Introduction Lipids are molecular organic compounds, composed largely of carbon, oxygen and hydrogen and are essential for cell growth. Lipids are non-soluble in water and combine with carbohydrates and proteins to form the majority of all plant and animal cells. Lipids are transported through the body attached to protein and are an important element of food. Lipids are the third major micronutrient required for the body after carbohydrates and protein. Lipids are also a medical term for ‘fat’ and though all fats are lipids, not all lipids are fats. Lipids have many roles within the body, one of its main duties is to transport, absorb and digest the fat soluble vitamins A,D,E and K. They provide the most energy to the body, are used for energy storage, cell membrane development, and they protect vital organs. Other major purposes are that they insulate layers under the skin, provide vitamins, essential nutrients and fatty acids in the body. Fatty acids cannot be made by the body and are therefore fat is essential in the diet. In many foods the lipid components add to flavor perception, texture, mouth feel and appearance. (Intro to food Nutrition handout, 15/02/11) It is important to remember that although fat is essential, the body only requires 30% of daily calorie intake to come from fat. Over consumption of...
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...UTAR FHSC1214 Fundamentals of Cell Biology Trimester 1 How YOU can do well in BIOLOGY Follow the 4A’s and you can expect A’s. A ttitude • Attend ALL lectures, tutorials and practicals on time without fail. • Be attentive in class and revise your notes after class while the topic is still fresh in your mind. Why waste time re-reading 2-3 months later? • Do your assignments faithfully as they carry marks for the finals. • Come prepared for lessons (i.e. read up beforehand). • Read up beforehand before attending lectures so that you won’t be lost and wasted hours of your life week after week. • Why stress yourself out if you can avoid it? Do NOT count on last minute revision for tests and examinations, as it will be too late to catch up and seek help in areas where you may find confusing or unclear of. • Why panic before exams because you can’t find this or that? Keep separate files for lecture, tutorial and practical. File up the respective notes systematically so that you do not lose them along the semester. • Do you expect the lecturer/ tutor to be available all the time to answer your questions? It is YOUR responsibility to take the initiative to clear your doubts or satisfy your curiosity to understand certain scientific phenomena by reading up on the relevant topics. A Based on a true story… A professor at the National University of Singapore recounts how on one occasion a student consulted him days before the exam. Student:...
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...HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The pronouns "I", "We" and "They" are not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the summary, the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized is preferred. For example, Staphylococcus aureus. The genus is capitalized but the species is not. After the full genus name is given in the paper, it can be written as S. aureus, but still italicized. This is as long as there in no other...
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