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Cell Process

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Submitted By runqi851216
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Cell Processes: Photosynthesis
Part 1: Pigments in Plant Leaves
Purpose: The purpose of this experiment is to visualize the pigments present in plant leaves by using paper chromatography.
Materials Used: Chromatography vial with cap, wax pencil, disposable transfer pipet, chromatography solvent, chromatography paper strip, sharp pencil, ruler, pair of scissors, piece of fresh pre-soaked spinach, coin, pair of forceps.
Observations and Data: Distance of Pigments From Original Pencil Line | | Pigment | Color | Migration Distance (mm) | Rf Value | | Carotene | Orange | 59 mm | .94 | | Xanthophyll | Yellow | 56 mm | .89 | | Chlorophyll a | Light Green | 29 mm | .46 | | Chlorophyll b | Dark Green | 14 mm | .22 | Solvent | | 63 mm | |

Analysis and Results: 1. Which pigment migrated the farthest on the chromatogram? Explain how this migration occurred.
According to the data Carotene travel furthest, this is because it was the most soluble and it didn't form bonds with the paper. 2. What does the Rf value represent? If you were to perform your experiment on a chromatography paper twice the length of the one used, would your Rf values still be the same?
Rf value represent the distance traveled by the pigments divided by distance traveled by solvent. The Rf value would stay the same because if the pigments travel further so will the solvent. 3. How do plant pigments and the absorption spectrum relate to photosynthesis?
In a plant there are various pigments, and the reason why there are so many of pigments is because each absorbs light more efficiently in different part of the spectrum. For example, chlorophyll a absorbs well when the wavelength is about 400-450 nm, and chlorophyll b absorbs well when wavelength is 600-650. In conclusion, plants have so many pigments is because it wants to absorb all the colors in the light

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