...2) Ecotoxicity testing on Daphnia magna Acute immobolisation test on Daphnia sp. following OECD guideline 202 In immobilization test, daphnids are exposed to the ENMs at the range of concentrations for 48 hrs. The immobilization of daphnids is indicated at 48 hrs and compared with control values. The percentages of immobilization against the concentration are plotted to determine the EC50 at 48 hrs. The procedure of testing is described as follows. a) D. magna at age of less than 24 hrs are used in this test; five healthy daphnids are placed into a test vessel filled with ISO test water and composite natural water for each ENM concentration and control. At least five concentrations of each ENM are tested. b) Daphnids are exposed to each concentration of each ENM for 48 hrs at...
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...Alcohol on Daphnia magna Yong Im El Centro College This experiment tested the effects of alcohol on the heart rate of Daphnia magna. We observed the heart rate of the D.magna in just water and compared it with the organism in a 5 percent alcohol solution. The results showed that the D.magna heart rate was decreased by 33 percent. The alcohol has an effect on the central nervous system. Introduction Alcohol is a common organic compound formed when a hydroxyl group (-OH) is substituted for a hydrogen atom in a hydrocarbon chain. A common form of alcohol is ethanol found in alcoholic beverages which is derived from fermentation of sugar with yeast. The anesthetic ether is also made from ethanol. Alcohol acts as a central nervous system depressant...
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...Heart Rate of a Daphnia Magna WCCC Tina Reott Anatomy Physiology II /SPRING SEMESTER 2015 Dr Matt Sisak Introduction In order for the body to operate properly, it is necessary for homeostasis, or a relatively stable environment, to be maintained. The cardiac center is the medulla oblongata regulates the heart rate to maintain a stable environment. It is composed of two neural pools. One is the cardioacceleratory center and the other is the cardioinhibitory center. Each regulates the heartbeat through the sympathetic cardiac nerves and the parasympathetic fibers of the vagus nerves respectfully. Many factors affect the heart rate including sensory and emotional stimuli as well as receptor input from proprioceptors (in...
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...drops of caffeine to six Daphnis for each temperature of the five different temperatures we are testing, 15°C, 20°C, 30°C, 35°C, and 40°C. after adding the caffeine, we calculated the BPM for each Daphnia individually, then the average for each temperature was taken. The independent variables that we manipulated in this experiment were the amount of the caffeine that was added to the Daphnia’s and the temperature of the water. The dependent variable we measured was the heartbeat of Daphnia’s per minute. One of the confounding variables was the concentration of the water in which the Daphnia’s was in. however; the same environment was used for all the Daphnia’s, which eliminates the effect of this variable in the result. The same light source and the same room temperature (baseline) were used in the all measurement of the...
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...Effect of Substances on the Heart Rate of a Daphnia Magna Lab Report Katherine Schmucker WCCC/Semester 2 A&P 2/Bio-072 Dr. Matthew Sisak February 6, 2015 Introduction: The substances that were applied to the Daphnia were aspirin, nasal spray, adrenalin, acetylcholine, and alka seltzer (College, p. 42). Aspirin’s active additive is acetyl salicylic acid (Henderson, n.d.). The nasal spray’s active additive is “therapeutic (Administration, 2002).” Adrenalin’s active additive is “acid tartrate (Agency, n.d.).” Acetylcholine contains “5-methyl tetrahydrofolate, huperzine A, and alpha-tocotrienol (Research, 2015).” Alka seltzer’s active additive include aspirin, sodium bicarbonate, and citric acid (Alka-Seltzer Effervescent Tablets Original,...
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...NATURE|Vol 461|1 October 2009 Vol 461|1 October 2009 RESEARCH HIGHLIGHTS JOURNAL CLUB Bruce R. Conklin Gladstone Institute of Cardiovascular Disease, San Francisco, California A geneticist wonders why we need to sleep. Scientists can have a love–hate relationship with sleep. We know that it is vital for our health, but not the reasons why. We celebrate dreams that provide inspiration, but often dismiss sleep as a chore. Yet deep sleep can provide insight into vexing problems. In 1920, pharmacologist Otto Loewi famously had a recurring dream that suggested how he could demonstrate neurotransmission in the lab. The key experimental details escaped him until he captured the dream in a bedside notebook. Later that day, he performed his Nobel-prizewinning experiments with the aid of a few frog hearts and a water bath. Now, a team led by Ying-Hui Fu reports that a single mutation in a gene called DEC2 can cause people to sleep for only about six hours per night instead of the usual eight (Y. He et al. Science 325, 866–870; 2009). This mutation seems to be exceedingly rare, with only two carriers found so far. Only by introducing this mutation into transgenic mice and fruitflies could the researchers show compelling evidence of the mutation’s effect. These two additional waking hours each day are quite remarkable when you consider that, over 80 years, this would add up to more than 8 years of extra productivity! Why are extreme short sleepers so rare? Surely evolutionary pressures...
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...Maria Vasilenko 223901 Feasibility of using biosensors for heavy metal detection in complex matrices such as bio-slurries. Master of Science Thesis Examiners: Professor Matti Karp Professor Raghida Lepistö Examiner and topic approved in The Science and Bioengineering Department Council meeting on 7.11.2012 Abstract TAMPERE UNIVERSITY OF TECHNOLOGY Master‟s Degree Programme in Science and Bioengineering Vasilenko Maria: Feasibility of using biosensors for heavy metal detection in complex matrices such as bio-slurries. Seminar paper, 97 pages November 2012 Major: Biotechnology Examiners: Matti Karp, Raghida Lepisto Keywords: environmental pollution, heavy metals, biosensors, slurries The quality of bioslurries that are used in industrial production and agriculture need to be watched very closely to avoid spreading of contaminants on area and poisoning of humans and animals. Because heavy metals are very stable and toxic in many chemical compositions, their amount should be estimated very thoroughly. A new approach that involved biosensors was tested in this study. Because the slurries are complex non-unified matrices which composed of two phases – solid and liquid, the cell behavior can varies a lot from the one that explained in water and so the estimation of ion concentration can be not reliable. It was shown that the cell actually behave different in the slurries. Normally the dissolved compounds suppress the biosensor activity and, in the same time, the ions in the...
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...A Review of Literature Related to Oil Spill Dispersants 1997-2008 for Prince William Sound Regional Citizens’ Advisory Council (PWSRCAC) Anchorage, Alaska by Merv Fingas Spill Science Edmonton, Alberta PWSRCAC Contract Number - 955.08.03 Disclaimer: The opinions expressed in this PWSRCAC-commissioned report are not necessarily those of PWSRCAC September, 2008 ii Abstract This report is a review of the literature on oil spill dispersants published from 1997 to August, 2008. The report identifies and focusses on recent advances in dispersant effectiveness, toxicity, and biodegradation. Other topics such as application, use, behaviour and fate are also covered. The prime motivation for using dispersants is to reduce the impact of oil on shorelines, thus the application must be successful and effectiveness high. As some oil would come ashore, discussion remains on what effectiveness is required to significantly reduce the shoreline impact. A major issue is the actual effectiveness during spills so that these values can be used in estimates for the future. The second motivation for using dispersants is to reduce the impact on birds and mammals on the water surface. The benefits of using dispersants to reduce impacts on wildlife still remain unknown. The third motivation for using dispersants is to promote the biodegradation of oil in the water column. The effect of dispersants on biodegradation is still a matter of dispute. Some papers state that dispersants inhibit...
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...GMO MYTHS AND TRUTHS An evidence-based examination of the claims made for the safety and efficacy of genetically modified crops Michael Antoniou Claire Robinson John Fagan June 2012 GMO Myths and Truths An evidence-based examination of the claims made for the safety and efficacy of genetically modified crops Version 1.3 by Michael Antoniou Claire Robinson John Fagan © Earth Open Source www.earthopensource.org 2nd Floor 145–157, St John Street, London EC1V 4PY, United Kingdom Contact email: claire.robinson@earthopensource.org June 2012 Disclaimer The views and opinions expressed in this paper, or otherwise published by EOS, are those of the authors and do not represent the official policy, position, or views of other organizations, universities, companies, or corporations that the authors may be affiliated with. GMO Myths and Truths 2 About the authors Michael Antoniou, PhD is reader in molecular genetics and head, Gene Expression and Therapy Group, King’s Cols: lege London School of Medicine, London, UK. He has 28 years’ experience in the use of genetic engineering technology investigating gene organisation and control, with over 40 peer reviewed publications of original work, and holds inventor status on a number of gene expression biotechnology patents. Dr Antoniou has a large network of collaborators in industry and academia who are making use of his discoveries in gene control mechanisms for the production of research, diagnostic and therapeutic products...
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...No. Nama Perguruan Tinggi AKADEMI AKUNTANSI PGRI JEMBER Nama Pengusul Sisda Rizqi Rindang Sari Program Kegiatan Judul Kegiatan 1 PKMK KUE TART CAENIS ( CANTIK, ENAK DAN EKONOMIS) BERBAHAN DASAR TAPE 2 AKADEMI FARMASI KEBANGSAAN Nensi MAKASSAR AKADEMI KEBIDANAN CITRA MEDIKA SURAKARTA AKADEMI KEBIDANAN GIRI SATRIA HUSADA AKADEMI KEPERAWATAN KERTA CENDIKA SIDOARJO AKADEMI KEPERAWATAN KERTA CENDIKA SIDOARJO AKADEMI KEPERAWATAN KERTA CENDIKA SIDOARJO Putri Purnamasari PKMK LILIN SEHAT AROMA KURINDU PANCAKE GARCINIA MANGOSTANA ( PANCAKE KULIT MANGGIS ) 3 PKMK 4 Latifah Sulistyowati PKMK Pemanfaatan Potensi Jambu Mete secara Terpadu dan Pengolahannya sebagai Abon Karmelin (Karamel Bromelin) : Pelunak Aneka Jenis Daging Dari Limbah Nanas Yang Ramah Lingkungan, Higienis Dan Praktis PUDING“BALECI”( KERES) MAKANAN BERSERATANTI ASAM URAT 5 Achmad PKMK Zainunddin Zulfi 6 Dian Kartika Sari PKMK 7 Radita Sandia PKMK Selonot Sehat (S2) Diit untuk Penderita Diabetes 8 AKADEMI PEREKAM Agustina MEDIK & INFO KES Wulandari CITRA MEDIKA AKADEMI PEREKAM MEDIK & INFO KES Anton Sulistya CITRA MEDIKA AKADEMI PEREKAM Eka Mariyana MEDIK & INFO KES Safitri CITRA MEDIKA AKADEMI PEREKAM MEDIK & INFO KES Ferlina Hastuti CITRA MEDIKA AKADEMI PEREKAM Nindita Rin MEDIK & INFO KES Prasetyo D CITRA MEDIKA AKADEMI PEREKAM MEDIK & INFO KES Sri Rahayu CITRA MEDIKA AKADEMI PERIKANAN YOGYAKARTA PKMK Kasubi Wingko Kaya Akan Karbohidrat...
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