...Liquid-liquid extraction, separation of the organic and aqueous layer was used to extract the supernatant or extract. Also, thin layer chromatography or TLC was used to separate the different lipid components by using the Rf values computed. The farther the distance traveled by the compound (higher Rf), the more nonpolar the component, while the smaller the distance traveled, the more polar the component (lower Rf). Lecithin and cholesterol was not able to travel the plate. After, the isolated lipid was subjected to qualitative tests such as Acrolein test, test for phosphates, Leibermann-Burchard test and test for unsaturation. Acrolein tests determine the presence of glycerin; the test for phosphate detects phosphate groups in the structure of the lipid, Leibermann-Burchard uses cholesterol as the standard and detects the presence of steroids and terpenes. The test for unsaturation on the other hand detects the presence of double bonds through halogenation reaction. The extract contains glycerol, phosphate, cholesterol and double bonds. Errors in the experiment can be attributed to the human error, false methodology and presence of contaminant in the reagent used....
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...Coconut Oil: Why it is Good For You In this article, fats and oils are used interchangeably but in a strict sense, oil usually means liquid at room temperature and fat usually means solid at room temperature. However, coconut oil is solid at temperatures under 76 degrees F. So if you live at temperatures of 76 degrees F or more, coconut oil is liquid; if less than 76 degrees F, coconut oil is a fat. Saturated fat – one that has a small degree of unsaturation or double bonds and tends to be more solid at room temperatures lower than 76 degrees F. Example: butter, coconut oil. Monounsaturated oil – Contains some saturated fat but is largely oleic acid, a mono-unsaturated oil, which contains only one double bond. Example: Olive oil. Polyunsaturated oils – poly means many, so this means that the fat has more than one double bond. Example: linoleic (omega-6) acid has two double bonds; alpha-linolenic (omega-3) acid has three double bonds; arachidonic acid has four double bonds. The following information comes from the research of Ray Peat, Ph.D. and Mary G. Enig, Ph.D. References are given where applicable. I recommend only three types of fats to my clients: extra virgin olive oil, organic or, preferably raw butter, and organic coconut oil. Most people understand the first two but cringe at the thought of eating coconut oil. Here’s why I recommend coconut oil to everyone. Coconut oil has been used as cooking oil for thousands of years. Popular cookbooks advertised...
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...their calorie content 5. During digestion, protein is broken down to amino acids, carbohydrate to glucose and fat to fatty acids and monoglycerides 6. The form of sugar important to the body as blood sugar is Glucose 7. Dietary Guidelines recommend that carbohydrate intake continue approximately what percent of the total caloric intake? 8. All of the following are benefits of fiber except: 9. What is the function of bile? Is produced by the liver, stored in the gall bladder, and acts as an emulsifier to suspend fat 10. You have once again just finished a light breakfast of pancakes, syrup and orange juice. Which of the following series of events proceeds upon digestion/absorption of the meal until it is near time for lunch? 1. Blood Glucose rises 2. High blood glucose stimulates the pancreas to release insulin 3. Insulin stimulates glucose into cells & storage as glycogen in the liver and muscles 4. The body’s cells use glucose; blood levels decline 5.Low blood glucose levels stimulates pancreas to release glucagon into bloodstream 6. Glucagon stimulates liver cells to break down glycogen & release glucose into the bloodstream 7. Blood glucose begins to rise. 11. What compound is composed of 3 fatty acids and glycerol? Triglycerides 12. In which form are most dietary lipids found? Triglycerides 13. Lipids differ in their degree of saturation or...
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...Growing Demand for Palm Oil in India With the world’s second largest population of 1.2 billion and its oils and fats consumption estimated at 17.8 million tonnes in 2010, India becomes one of the world’s leading oils and fats economies. India is one of the largest producers of oilseeds in the world, both in terms of the area and output. However, India is the third world’s leading importer of oils and fats in 2010. India’s oils and fats production have not been able to keep pace with the large demand in the country. Estimates indicate that while the oils and fats output grew at about two percent per annum over the past four years, consumption has grown at some seven percent per annum; hence import of oils and fats is necessary to fill the gap between production and consumption. The total consumption of oils and fats for 2010 was 17.8 million tonnes while local production was only able to cater 9.0 million tonnes. Palm Oil – Continue to be Major Importing Oil by India Palm oil has been the major imported oil by India which has become the world’s largest importer of palm oil. In 2010, a total of 6.58 million tonnes of palm oil were imported and it constituted about 70% of India’s total imports. Most of the palm oil imported into India is in the form of crude palm oil. The main two exporters of palm oil to India are Malaysia and Indonesia with Indonesia having the market share of 80% approximately. The large market share of palm oil in India’s oils and fats imports is driven by...
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...LIPIDS CHEMICAL COMPOSITION OF LIPIDS Lipids are a group of substances made up of carbon, hydrogen and oxygen but in different ratios from those of carbohydrates. Lipids have more carbohydrates and hydrogen in proportion to their oxygen. The lower the amount of oxygen in relation to carbon and hydrogen results in fats being a more concentrated source of energy than carbohydrates. Most of the energy from fats is provided by a class of lipids called triglycerides. These are lipids that are made up of two components i.e. Glycerol (a trihydric alcohol and fatty acid). Fatty Acids Almost all naturally occurring lipids yield fatty acids on hydrolysis. Some yield only one fatty acid per molecule while others may yield as many as three fatty acids. In other words, fatty acids are the building blocks of lipids. Fatty acids are compounds that are composed of an even numbered carbon chain of from about 4-20 carbon atoms in the length. They have a single carboxyl group and a long hydro-carbon chain which is the one responsible for the oily nature of the lipids. The long hydrocarbon chain is said to be hydrophobic because of its non-polar characteristics while the carboxyl end is said to be hydrophilic because it is polar (negatively charged). Fatty acids with no double bonds in their structural are called Saturated fatty acids and they include: Butryric acid (Butatonoic acid C-4) Caproic acid (Hexanoic C-6) Capric acid (Octanoic acid C-8) Fatty acids are named on...
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...Organic Virgin Coconut Oil - A Healthy Oil The Coconut Oil Story Virgin Coconut Oil: An introduction For about 3960 years of the of the past 4000 years of the documented historical use of the fruits of the coconut palm as a food and a pharmaceutical, the news has all been good. It was seen as a sustainable resource from which the harvested materials influenced every aspect of the lives of tropical communities, but most importantly its fruit, the coconut flesh, water, milk and oil. The use of coconut oil around the world in tropical regions is prolific: South and Central America, Africa, the Indian subcontinent, Micro-, Mele- and Poly-nesia and most of Asia. The uses are so respected that they were documented by Ayurvedic medicine in Sanskrit from 1500BC in all areas relating to the mind, body and spirit. Early European explorers including Captain Cook wrote affectionately about the beauty of communities across the Pacific using coconut oil as an integral part of their daily lives. During WWII the water of the young green coconut was successfully used as a substitute for a saline drip saving the lives of many allied soldiers. After the war, in England coconut oil was sold as "margarine" and in the USA as "coconut butter". However, this all changed in 1954. In the social environment of the USA in which coronary heart disease (CHD) was becoming the primary cause of mortality in the adult population, the young researcher David Kritchevsky published two academic papers. The initial...
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...value of vegetable oils PIEB II – MIB|MIEQ Yen Nguyen-MESG Index Abstract 3 Introduction 4 Methods and Materials 6 Results and Discussion 7 Acidity 7 Peroxide 9 Conclusion 11 References 12 Annex 14 Abstract Nowadays the quality of commercial oil is very concerned by the customers, as oxidative stability is one of the most important factors in keeping the quality of oils, fatty acids in neutral fraction, peroxide values and induction period were evaluated as stability parameters in vegetable oil, in order to detect oxidative changes during cooking, this study performed an experiment to determine the changing in acidity values and peroxide values of one vegetable oil sample in different treatment conditions. Both acidity values and peroxide values were determined using standard titration methods appropriate for each indicator. All acidity values were in range 0.7817 – 0.9138 mg KOH/kg oil and this is below the recommended maximum for acidity index, it means that the oil is accepted for consumption. However there were no significant different between samples in different temperature, this conflicts with some other studies that demonstrated the significant effect of temperature on acid value and oleic acid values. The values of peroxide were in the range of 4.14-5.87 meq/kg oil and also in the acceptable range for peroxide value. The results showed that with a small amount of BHT added (20mg/g oil) it had a better affect to the oil in short time heated...
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...ANALYSIS OF FOOD PRODUCTS FOOD SCIENCE 581 Instructor Prof. D. Julian McClements Room 238, Chenoweth Lab Tel: 545 1019 Email: mcclements@foodsci.umass.edu Course Description Food products are analyzed for a variety of reasons, e.g., compliance with legal and labeling requirements, assessment of product quality, determination of nutritive value, detection of adulteration, research and development. The lectures will cover the basic principles of analytical procedures and techniques commonly used to provide information about the chemical composition, structure and physical properties of food materials. The aim of the laboratory classes is to give students experience in performing food analysis experiments, analyzing data and reporting their findings. In addition, students are expected to work in teams on a special project where they will identify and critically assess the most appropriate analytical methods for analyzing the properties of a particular food product. [4 Credits]. Textbooks * Introduction to Food Analysis. S.S. Nielsen, 1998. Aspen Publishers - The best general overview of food analysis techniques currently available. (Required). * Food Analysis: Theory and Practice. Y. Pomeranz and C.E. Meloan, Chapman and Hall - General overview of food analysis techniques (Useful) * Food Analysis: Principles and Techniques. D.W. Gruenwedel and J.R. Whitaker, Marcel Dekker - General overview of food analysis techniques (Useful) * Analytical Chemistry of Foods...
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...enzyme class 3 and have been assigned sub-sub class 3.1.1 due to their specificity for carboxylic acid ester bonds. Lipases (triacylglycerol acylhydrolases, E.C. 3.1.1.3) are ubiquitous enzymes of considerablephysiological significance and industrial potential. Lipases catalyze the hydrolysis of triacylglycerols to glycerol and free fatty acids. In contrast to esterases, lipases are activated only when adsorbed to an oil–water interface (Martinelle et al., 1995) and do not hydrolyze dissolved substrates in the bulk fluid. A true lipase will split emulsified esters of glycerine and long-chain fatty acids such as triolein and tripalmitin. Lipases are serine hydrolases and contain the consensus sequence G – X1 – S – X2 – G as the catalytic moiety, where G – glycine, S – serine,X1 – histidine and X2 – glutamic or aspartic acid .Lipases display little activity in aqueous solutions containing soluble substrates. In contrast, esterases show normal Michaelis–Menten kinetics in solution. In eukaryotes, lipases areinvolved in various stages of lipid metabolism including fat digestion, absorption, reconstitution, and lipoprotein metabolism. In plants, lipases are found in energy reserve tissues.How lipases and lipids interact at the interface is still not entirely clear and is a subject ofintense investigation (Balashev et al., 2001). Sources of Lipase:...
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...CHEMISTRY CHEMISTRY (CLASSES XI–XII) Rationale Higher Secondary Stage is the most crucial stage of school education because at this stage specialised discipline based, content oriented courses are introduced. Students reach this stage after 10 years of general education and opt for Chemistry with a purpose of mostly for pursuing their career in basic sciences or professional courses like medicines, engineering, technology and studying courses in applied areas of science and technology at tertiary level. Therefore, at this stage, there is a need to provide learners with sufficient conceptual background of Chemistry, which will make them competent to meet the challenges of academic and professional courses after the higher secondary stage. National Curriculum Framework for School Education – 2005 recommends a disciplinary approach with appropriate rigour and depth with the care that syllabus is not heavy and at the same time it is comparable to the international level. It emphasizes a coherent focus on important ideas within the discipline that are properly sequenced to optimize learning. It recommends that theoretical component of Higher Secondary Science should emphasize on problem solving methods and the awareness of Syllabus for Secondary and Higher Secondary Levels 22 historical development of key concepts of science be judiciously integrated into content. The present exercise of syllabus development in Chemistry at Higher Secondary Stage is based on this framework...
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...Journal of Controlled Release 113 (2006) 189 – 207 www.elsevier.com/locate/jconrel Review Role of antioxidants in prophylaxis and therapy: A pharmaceutical perspective D. Venkat Ratnam, D.D. Ankola, V. Bhardwaj, D.K. Sahana, M.N.V. Ravi Kumar ⁎ Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Phase-X, S.A.S. Nagar, Mohali, Punjab, India - 160062 Received 31 January 2006; accepted 26 April 2006 Available online 13 May 2006 Abstract Antioxidants are emerging as prophylactic and therapeutic agents. These are the agents, which scavenge free radicals otherwise reactive oxygen species and prevent the damage caused by them. Free radicals have been associated with pathogenesis of various disorders like cancer, diabetes, cardiovascular diseases, autoimmune diseases, neurodegenerative disorders and are implicated in aging. Several antioxidants like SOD, CAT, epigallocatechin-3-O-gallate, lycopene, ellagic acid, coenzyme Q10, indole-3-carbinol, genistein, quercetin, vitamin C and vitamin E have been found to be pharmacologically active as prophylactic and therapeutic agents for above mentioned diseases. Antioxidants are part of diet but their bioavailability through dietary supplementation depends on several factors. This major drawback of dietary agents may be due to one or many of the several factors like poor solubility, inefficient permeability, instability due to storage of food, first pass effect and GI degradation. Conventional...
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...CARIBBEAN EXAMINATIONS COUNCIL Caribbean Secondary Education Certificate CSEC® CHEMISTRY SYLLABUS Effective for examinations from May–June 2015 CXC 21/G/SYLL 13 Published by the Caribbean Examinations Council. All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form, or by any means electronic, photocopying, recording or otherwise without prior permission of the author or publisher. Correspondence related to the syllabus should be addressed to: The Pro-Registrar Caribbean Examinations Council Caenwood Centre 37 Arnold Road, Kingston 5, Jamaica Telephone Number: + 1 (876) 630-5200 Facsimile Number: + 1 (876) 967-4972 E-mail Address: cxcwzo@cxc.org Website: www.cxc.org Copyright © 2013 by Caribbean Examinations Council The Garrison, St Michael BB14038, Barbados CXC 21/G/SYLL 13 Contents RATIONALE ................................................................................................................................... AIMS ............................................................................................................................................. CANDIDATE POPULATION ............................................................................................................. SUGGESTED TIME-TABLE ALLOCATION ........................................................................................ ORGANISATION OF THE SYLLABUS .................................................
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...Employment News 31 May - 6 June 2014 www.employmentnews.gov.in 21 UNION PUBLIC SERVICE COMMISSION EXAMINATION NOTICE NO. 09/2014-CSP (LAST DATE FOR RECEIPT OF APPLICATIONS : 30/06/2014) DATE :31.05.2014 CIVIL SERVICES EXAMINATION, 2014 (Commission’s website-http://upsc.gov.in) F. No. 1/5/2013-E.I(B) : Preliminary Examination of the Civil Services Examination for recruitment to the Services and Posts mentioned below will be held by the Union Public Service Commission on 24th Aug., 2014 in accordance with the Rules published by the Department of Personnel & Training in the Gazette of India Extraordinary dated 31st May, 2014. (i) Indian Administrative Service. (ii) Indian Foreign Service. (iii) Indian Police Service. (iv) Indian P & T Accounts & Finance Service, Group ‘A’. (v) Indian Audit and Accounts Service, Group ‘A’. (vi) Indian Revenue Service (Customs and Central Excise), Group ‘A’. (vii) Indian Defence Accounts Service, Group ‘A’. (viii) Indian Revenue Service (I.T.), Group ‘A’. (ix) Indian Ordnance Factories Service, Group ‘A’ (Assistant Works Manager, Administration). (x) Indian Postal Service, Group ‘A’. (xi) Indian Civil Accounts Service, Group ‘A’. (xii) Indian Railway Traffic Service, Group ‘A’. (xiii) Indian Railway Accounts Service, Group 'A'. (xiv) Indian Railway Personnel Service, Group ‘A’. (xv) Post of Assistant Security Commissioner in Railway Protection Force, Group ‘A’ (xvi) Indian Defence Estates Service, Group...
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...Employment News 11 - 17 February 2012 www.employmentnews.gov.in 21 Union Public Service Commission EXAMINATION NOTICE NO. 04/2012-CSP DATED 11.02.2012 (LAST DATE FOR RECEIPT OF APPLICATIONS : 05.03.2012) CIVIL SERVICES EXAMINATION, 2012 (Commission's website - http://www.upsc.gov.in) F. No. 1/4/2011-E.I(B) : Preliminary Examination of the Civil Services Examination for recruitment to the Services and Posts mentioned below will be held by the Union Public Service Commission on 20th May, 2012 in accordance with the Rules published by the Department of Personnel & Training in the Gazette of India Extraordinary dated 4th February, 2012. (i) Indian Administrative Service. (ii) Indian Foreign Service. (iii) Indian Police Service. (iv) Indian P & T Accounts & Finance Service, Group ‘A’. (v) Indian Audit and Accounts Service, Group ‘A’. (vi) Indian Revenue Service (Customs and Central Excise), Group ‘A’. (vii) Indian Defence Accounts Service, Group ‘A’. (viii) Indian Revenue Service (I.T.), Group ‘A’. (ix) Indian Ordnance Factories Service, Group ‘A’ (Assistant Works Manager, Administration). (x) Indian Postal Service, Group ‘A’. (xi) Indian Civil Accounts Service, Group ‘A’. (xii) Indian Railway Traffic Service, Group ‘A’. (xiii) Indian Railway Accounts Service, Group 'A'. (xiv) Indian Railway Personnel Service, Group ‘A’. (xv) Post of Assistant Security Commissioner in Railway Protection Force, Group ‘A’ (xvi) Indian Defence Estates Service, Group ‘A’. (xvii) Indian Information...
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...Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 17 Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Introduction to Biochemistry Water Amino Acids and the Primary Structures of Proteins Proteins: Three-Dimensional Structure and Function Properties of Enzymes Mechanisms of Enzymes Coenzymes and Vitamins Carbohydrates Lipids and Membranes Introduction to Metabolism Glycolysis Gluconeogenesis, The Pentose Phosphate Pathway, and Glycogen Metabolism The Citric Acid Cycle Electron Transport and Oxidative Phosphorylation Photosynthesis Lipid Metabolism Amino Acid Metabolism Nucleotide Metabolism Nucleic Acids DNA Replication, Repair, and Recombination Transcription and RNA Processing Protein Synthesis Recombinant DNA Technology 1 10 27 46 65 85 104 119 137 153 169 185 199 213 227 241 256 269 284 300 315 330 348 Chapter 1 Introduction to Biochemistry 1) Which elements account for more than 97% of the weight of most organisms? A) C, H, N, Mg, O, S B) C, H, N, O, P, S C) C, H, N D) Fe, C, H, O, P E) Ca2+ , K+ , Na+ , Mg 2+ , ClAnswer: B Page Ref: Section 2 2) Proteins in biological membranes may be A) porous. B) attached to the membrane surface. C) span the membrane. D) All of the above E) B and C only Answer: D Page Ref: Section 3 3) Which statement...
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