Bi. What are the tissue types of each of the 5 layers of the cornea?
The Epithelium consists of stratified epithelial tissue, the Bowman’s layer consists of connective tissue (more specifically Collagen fibres), the Stroma consists also of connective tissue (again Collagen fibres but also keratocytes), Descemet’s membrane is a basement membrane which is a type of epithelial tissue and the Endothelium is epithelial tissue.

Bii. What are the functions of each of the 5 layers of the cornea?
Epithelium:
-The Epithelium acts as a protective layer to the eye. A type of barrier that blocks the movement of molecules such as dust from one side of the layer to the other.
-Due to consisting of non-keratinised squamous cells, it displays a very smooth …show more content…
What is Descemet’s membrane?
Descemet’s membrane is the basement membrane of the Endothelium. It is made up of epithelial tissue. It is produced constantly over a persons lifetime so will thicken.
It consists of two laminae:
-Anterior laminae
-Poster laminae
Descemet’s is very resistant to trauma but also has the ability to regenerate.
Remington LA. Clinical Anatomy & Physiology of the Visual System 3rd edition, page 18

Bvii. What important role does the endothelium play in the physiology of the cornea?
The endothelium is leaky. This incomplete barrier allows the entrance of nutrients. Water will pass out of this incomplete barrier and water will be actively transported out. The fluid needs to be drained for the Stroma and the cornea as a whole to be transparent. This obviously means that the Endothelium plays a massively important role as without it, the layers and cells wouldn’t receive the nutrients they require to function properly and without the drainage of fluid, the eye would be almost useless as the surfaces that should be transparent, would be cloudy.
Remington LA. Clinical Anatomy & Physiology of the Visual System 3rd edition, pages 18-20

External Limbus

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A. What are the principles of histological staining?
We stain cells so they are visible under the microscope, so we can clearly see the different structures in the cell when using the microscope. Also many biochemical reactions may occur, letting us identify structures or organisms.
B. Explain the term H&E, which applies to the staining technique used in the eye.sis slide.
H&E stands for Hematoxylin and Eosin. This is the dye used to stain the ‘eye.sis’ slide. It is the most commonly used stain for general histological preparations. This dye causes parts to be dyed shades of either blue or pink.
Dongmei Cui, John P. Naftel, James C. Lynch, Gongchao Yang, William P. Daley, Duane E. Haines, and Jonathan D. Fratkin. Atlas of Histology with functional and clinical corrections 1st edition.
C. How will the colours present in tissue with H&E staining relate to different types of tissue?
The parts of the cell that contain chromatic material such as RNA and DNA will be stained blue due to the Hematoxylin. The rest of the cell will pick up the pink stain as the eosin will bind to proteins.
Skip Brown, M.Div, HT (ASCP).The Science and Application of Hematoxylin and Eosin