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Gram Stain Lab Report

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In order to figure out what family an organism belongs to all the way down to the species, a scientist will perform many tests. Each test done will help to eliminate other bacteria. Once a scientist knows the species, the right steps can be taken to help out people if they get contaminated with the bacteria.

When I received my unknown bacteria, I started of doing a Gram Stain. This test revealed to me that I had a Gram negative bacilli in a random arrangement. This step is very important to figure out what type of family the bacteria belongs to. Gram negative bacteria have a thin peptidoglycan layer and a waxy outer layer. This is important to know when it comes down to giving antibiotics. Most bacteria are susceptible to antibiotics …show more content…
I inoculated 2 TSA plates. I placed one into a 4C environment and the second one in a 40C incubator. To my surprise, the plate in the 4C had moderate growth while the place in 40C incubator had not growth visible. The plate in the 40C incubator most likely had the proteins denatured. The same thing can happen to humans when temperature in body becomes too high. Proteins in the brain can become denatured and we don’t want that to ever occur. Being able to breakdown starch is important to our bodies. We eat so many foods that contain starch. We need to break it down, use it or store it for later use. Starch gives our cells energy. Starch is stored in our body in the form of glycogen which can be pulled later for energy when needed. My bacteria is unable to breakdown starch meaning it doesn’t contain the enzyme amylase.

Many cells in our body including blood cells will not be able to survive high salt concentrations. This will cause our cells to crenate, taking out all water possible in the cell. Water is an essential part of our life. Humans are made mostly up by water. My bacteria was unable to grow in high salt concentration of 8.5%. For humans, .9% NaCl solution is equal to the tonicity of our blood …show more content…
To break it down, it means a false unit. Pseudo, meaning false, was derived from Greek, while monas is from Latin, meaning ‘a single unit.’ “Pseudomonas putida is capable of causing pneumonia, catheter-related blood stream infection, acute cholecystitis, cholangitis, tonsillitis, thrombophlebitis and skin and soft tissue infections” (Thomas, Okamoto, Bankowski & Seto 2013). That’s a scary list of infection/inflammations Pseudomonas putida capabilities in humans. Pseudomonas putida bacteremia was documented 28 times. Out of those 28 cases, 92.9% were cured with appropriate antibiotics. There’s a higher chance of catching this type of microbe with having an indwelling device. Some of the Pseudomonas putida strains can form biofilms making it hard for antibiotics to kill it (Molina 2014). Pseudomonas putida is a common bacteria living in water and soil. Pseudomonas putida also has its upside. “Pseudomonas putida can break down many types of hydrocarbons and organic toxins. In that manner, Pseudomonas putida helps clean up our environment” (Kwok). Even though this type of bacteria is capable of even leading to death, it can help to clean up our environment. Just because we hear the word bacteria, doesn’t mean we need to jump up, run and use an antibacterial. Every person has a normal microbiota, which helps with keeping out new bacteria’s due to limited space and the normal microbiota is already using up the nutrients available.

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