...Sean Cui Biology Formal Lab Report 2 Intro Throughout this lab, the main topic is genetic transformation. Genetic Transformation is a phenotypic change caused by genotype change from transferring foreign genes into an organism. This lab is to transform the bacteria E.Coli with the GFP gene (Green Fluorescent Protein). GFP makes organisms glow in the dark with UV light, similar to jellyfishes.GFP will be transferred into E.Coli through the use of recombinant DNA, or simply a vector. A vector is a plasmid that transfers foreign DNA into cells. Bacteria can transfer plasmid to other bacteria so they can survive and adapt to the environment they are in. The pGLO plasmid includes the GFP gene (allows bacteria to glow), Beta-lactamase (resists ampicillin), ORI sites (allows bacteria to self replicate), and AraC (allows induction of GFP gene). Materials and Methods: In this lab, first there will be two micro test tubes, one labled pGLO+ and the other pGLO-. Using a sterile transfer pipet, transfer 205 microliters of CaCl2 into each tube. Then put the tubes on ice. Then use a sterile loop to pick up a single colony of bacteria from the starter plate. Put the colony in the pGLO+ tube and spin the loop. Then put the tube back in ice. Repeat this for the pGLO- tube with a new sterile loop. Next use a new sterile loop and get some DNA stock. With the loop, immerse it in only in the pGLO+ tube. After that, incubate the tubes in ice for 10 minutes. Label one plate LB/amp +pGLO...
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...Revistas Científicas de América Latina, el Caribe, España y Portugal Sistema de Información Científica Duangporn Kantachote, Pakorn Prachyakij, Wilawan Charernjiratrakul, Metta Ongsakul, Yodsawee Duangjitcharoen, Chaiyavat Chaiyasut, Teruhiko Nitoda, Hiroshi Kanzaki Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Electronic Journal of Biotechnology, vol. 13, núm. 5, 2010, pp. 1-15, Pontificia Universidad Católica de Valparaíso Chile Available in: http://www.redalyc.org/articulo.oa?id=173318799002 Electronic Journal of Biotechnology, ISSN (Electronic Version): 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile How to cite Complete issue More information about this article Journal's homepage www.redalyc.org Non-Profit Academic Project, developed under the Open Acces Initiative Electronic Journal of Biotechnology ISSN: 0717-3458 http://www.ejbiotechnology.info DOI: 10.2225/vol13-issue5-fulltext-1 Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Duangporn Kantachote1 1 · Pakorn Prachyakij1 · Wilawan Charernjiratrakul1 Metta Ongsakul · Yodsawee Duangjitcharoen2 · Chaiyavat Chaiyasut2 Teruhiko Nitoda3 · Hiroshi Kanzaki3 1 Department of Microbiology, Faculty of Science, Prince of Songkla University, Thailand...
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...The materials used in the transformation of Escherichia Coli include a transformation solution, a starter plate of bacteria colonies, and pGLO. A bacterial suspension is created by transferring 250 μl of transformation into two sterile 2.0 microcentrifuge tubes of different colors. One of the tubes is labeled +pGLO and the other is labeled –pGLO. Once 250 μl transformation solution is in each of the tubes they are placed on a round floating tube rack and then placed on ice. To complete the bacterial suspension, 2 large or 3 small colonies of bacteria are taken from the starter plate using a sterile loop. Holding the +pGLO tube between two fingers, the loop with the bacterial colonies is placed in the transformation solution. To ensure that the entire colony is completely dispersed in the transformation solution the loop is spun vigorously until there are no floating chunks remaining in the solution. Using a new sterile loop, this same process is...
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...microcontroller Intallation of Arduino Microcontroller 1 Abstract This lab demonstrates the ability to assemble a digital system according to instructions. Chapter 1 Arduino Microcontroller 1.1 Introduction The Arduino Uno is a microcontroller board based on the ATmega328 (datasheet). It has 14 digital input/output pins (of which 6 can be used as PWM outputs), 6 analog inputs, a 16 MHz ceramic resonator, a USB connection, a power jack, an ICSP header, and a reset button. It contains everything needed to support the microcontroller; simply connect it to a computer with a USB cable or power it with a AC-to-DC adapter or battery.. 1.2 Aim The aim of this lab was to install and get familiar with the operation of Arduino microcontroller. 1.3 Objectives The objectives of this lab are as follows: • Demonstrate the ability to assemble a digital system according to instructions • Demonstrate the operation of the Arduino Basis Board by running sample programs. 1 1.4 Apparatus • Arduino Micro comotroller kit • Personal Computer (PC) 1.5 Proceedures • Collected the Arduino microcontroller kit • Read the instructions • Followed the instructions to demonstrate the it was working well • Took 2 sample programs to verify that it was working well 2 Chapter 2 Assembling of the Arduino microcontroller 2.1 Introduction The arduino starter kit includes a pre-cut easy to assemble wooden base. It is build by following...
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...Background Information: In this lab, the different positions of a battery powered toy car moving in one direction over time (Velocity) was measured and recorded. Time and distance were the two physical quantities measured in this lab. A stopwatch and a meter stick were used to measure the distance the toy car travelled in a specific amount of time. Once the data points were collected and plotted, the relationship between the points was found using a line of best fit, which was in the form of y=mx+b. Experimental Design: The purpose of this lab was to determine the equation relating the position to time of a moving battery powered car. The independent variable was the time (measured in seconds, with a stopwatch) throughout which the toy car travelled and the dependent variable was the how far (measured in meters, with a meter stick) the toy car travelled (its position). The toy car remained constant throughout the experiment. The hypothesis was that as the time increases, the toy car will continue to move forward at a constant velocity linearly. Methods: The lab was carried out in a fairly wide area so the car could have enough room to move and so that the lab members and other members of the classroom would not step on the toy car or trip over it, both of which could result in injuries. After a long and wide enough area was cleared to carry out the lab, team members were assigned tasks to do during the lab; these tasks included a timer, a car starter, and two people who marked...
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...May 17, 2013 1. Video: Windows Server 2008: How to Install a Printer: The Windows Server 2008 video was interesting to say the least; however sound or narration to the video would have been more helpful. Since it is silent it is difficult at times to ascertain exactly what the demonstrator is trying to communicate. At times the instructor was pointing to menu lists even though these were not part of the process. The process pretty much followed the same process that the lab book suggested in fact I had just finished the lab when I started watching the video. The only difference here was that the setup was for adding the printer on a Standard TCP/IP Port, which wasn’t covered in the lab book. Typing a printer name also selected the same as the port name. The instructor did copy/paste the IP address and Port Name, which did make that part seem much easier as well. Strange though the device wasn’t detected on the network and additional information was asked if the instructor. A generic network card was selected and then the printer still wasn’t located but the Add Printer window appeared and allowed the instructor to select the printer out of hundreds of others. Finally, an IBM 4029 laser printer was selected and of course the computer prompted for the driver disk immediately. Then a name for the printer was typed in, and then the installation process continued. Afterwards the Printer Sharing window appeared and the instructor entered the information required for sharing...
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...CS1100: Excel Lab 1 Filtering and Summarizing Data To complete this assignment you must submit an electronic copy to Blackboard by the due date. Use the data in the starter file. In this lab you are asked to filter and calculate various descriptive statistics on a data set about students at a university and calculate various descriptive statistics on a data set about loans and investments. In addition, you will solve a problem using induction. Problem 1 (25 Points) In the sheet “Problem 1” you are given a list of festivals, their locations and the seasons that they fall in. Your task is: 1. Using IF, filter the data for these festivals by season. You will need to add a column for each season and then use the IF function to filter the data by season. Your formula should use proper anchoring so it is copyable both down and across. 2. Calculate the total number of festivals each season as well as the percentage of festivals that fall in each season. Your solution must look like this (including the formatting): Problem 2 (40 Points) Your solution must take into account the possibility that the data could change, i.e., do not “hard code” any data values. If the season of a festival changes, your calculations should update to the correct values automatically. Additionally, if new rows are inserted or existing rows are deleted, your spreadsheet model should still work. In the sheet “Problem 2”, you are given information about a savings account. Use this information to create...
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...The Foreign DNA used in this experiment contains the gene AmpR, which makes the transformed bacteria Ampicillin resistant. The plasmid also contains a gene for GFP, which would allow the bacteria to glow under UV light. The hypothesis in this lab states that if the E. Coli cell is accompanied by the pGLO plasmid, the cell will grow in environment containing Ampicillin, but if only containing Ampicillin without the plasmid growth would be inhibited. The other part of the hypothesis explains that in an environment containing Ampicillin, Arabinose, and the plasmid, the bacteria would both grow and glow. At the end of the experiment, the results proved that E. Coli could be transformed using foreign plasmid DNA to form Ampicillin resistance. INTORDUCTION Genetic Engineering is important in several fields of biotechnology and is bigger than ever in improving human insulin. There are many examples today on the use of genetic engineering and biotechnology. First and foremost is creating bacteria that improve human insulin; others are frost and pest resistance in agriculture and the breakdown of pollutants involved in bioremediation. Transformation is described as the uptake of naked DNA in the environment by a recipient bacterium (Bio 1162 lab manual). The foreign DNA contains the desired genetic material that is inserted into the host organism....
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...part in the justice field. They collect, preserve, and interpret evidence found at crime scenes. Their job is to reconstruct the crime from the evidence. To become a criminalist one must obtain a Bachelor’s degree in biological, physical, or forensic sciences, as well as have accumulated a minimum of twenty-four semester hours of chemistry, biology and math. (Becoming a Criminalist: How to become a Criminalist, n.d.) Criminalist can work in the public sector in local police departments or sheriff departments, on a regional level at state crime labs and medical examiners offices and at the federal level for the FBI, DEA, and CIA. They may also teach at public colleges and universities. If I were to choose to work in the public sector, I would choose a state lab to be able to sort through evidence and help interpret the facts of the crime. Criminalist may also find similar work in the private sector working for independent forensic labs as well as teaching at private schools. These would be jobs that would have a smaller workforce. (Cornett, n.d.) If choosing a career in the private sector I would choose to teach at a private university and impart the knowledge and skills needed for...
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...Uses of Statistical Information in the Workplace HCS 438 November 9, 2011 Uses of Statistical Information in the Workplace The collection, interpretation, analysis and presentation of data can be used in the workplace (Bennett, Briggs, & Triola, 2009). Predictions are made based on the data collected. The workplace is a healthcare organization, such as on a post-partum unit provide opportunities to collect, organize, observe and interpret information regarding this population. The purpose of this paper is to determine which methods of statistics are used as a measuring tool and what levels of measurements are used or could be used to improve the decision-making process for this organization. Statistics in the Workplace Many areas of the hospital facility use statistics in measurement. How do statistics affect this hospital healthcare facility in women services? One example is measuring nurse/patient ratios based on patient census and acuity. Patient acuity and census determines how many nurses are assigned to each patient based on their acuity. This also ensures patient and personnel safety. Patient satisfaction surveys are performed to improve patient care outcome as measured by HCAHPS (Hospital Consumer Assessment of Healthcare Providers and Systems); these tactics were discovered by the Studer Group. HCAHPS encourages leaders and staff members alike to realize the results are meaningful and that they translate to better, more consistent quality care, which...
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...“Streptokinase is an enzyme mixture that is useful in clearing blood clots that occur in the heart and the lower extremities. Another useful enzyme for dissolving blood clots that occur as a result of heart attacks is human tissue plasminogen activator (TPA). Recombinant TPA is produced by genetic engineering techniques, using bacteria cultures to produce large quantities of human TPA 3” (Shields). In the first lab assignment about penicillin—How Penicillin Was Discovered, the lesson talked about how mold must be producing a chemical that killed bacteria. Turns out that an enzyme inhibitor used as a drug is penicillin. The way that an enzyme inhibitor acts as a penicillin, is that it will cross-link different “strands of the wall and is catalyzed by the enzyme glycopeptide trans-peptidase. The shape of penicillin resembles that of the normal substrate of glycopeptide trans-peptidase, so that penicillin binds to the active site of the trans-peptidase enzyme. Once bound to the active site, penicillin forms a permanent bond with one of the amino acid residues. This chemical reaction permanently inhibits the glycopeptide trans-peptidase enzyme, thus preventing...
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...Mechatronics BASIC MEASUREMENT TOOLS MTX 103 Fall 2 Credits 11 hours of lecture - 22 hours of lab Fundamentals of measurement tools. Topics include basic measurement, S.I. and U.S. customary measurement, precision measurement tools and dimensional gauging. Prerequisite: A grade of "C" or better in ELEC 101, 102, and 121; or concurrent enrollment in ELEC 101, 102, and 121; or consent of Instructional Unit. BASIC HYDRAULICS MTX 105 Fall 2 Credits 11 hours of lecture - 22 hours of lab Fundamentals of hydraulics. Topics include hydraulic power systems, hydraulic circuits, principles of hydraulic pressure and flow and various types of hydraulic valves. Prerequisite: A grade of "C" or better in ELEC 101, 102, and 121; or concurrent enrollment in ELEC 101, 102, and 121; or consent of Instructional Unit. BASIC PNEUMATICS MTX 107 Fall 2 Credits 11 hours of lecture - 22 hours of lab Fundamentals of pneumatics. Topics include pneumatic power systems, basic pneumatic circuits principles of pneumatic pressure and flow and pneumatic speed control. Prerequisite: A grade of "C" or better in ELEC 101, 102, and 121; or concurrent enrollment in ELEC 101, 102, and 121; or consent of Instructional Unit. ELECTRIC MOTOR CONTROL 1 MTX 110 Fall 4 Credits 22 hours of lecture - 44 hours of lab Fundamentals of electric motor control. Topics include electrical safety, control transformers, overload protection, ladder logic, control relays, electronic sensors, and other topics related...
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...The purpose of this lab was to genetically transform bacterial plasmid DNA with foreign pieces of DNA, pGLO, that causes expression of an ampicillin resistant gene, and see the effects it has on bacterial growth in presence of the antibiotic, ampicillin. We inserted pGLO DNA into the genome of the bacteria through the use of inoculation loops, Laurel Broth, transformation solution, and procedures such as heat shock and incubation. This experiment involved four bacterial agar plates, two of which we genetically transformed (experimental groups; +pGLO LB/amp/ara and +pGLO LB/amp), and two of which we did not genetically transform and were control plates (-pGLO LB and -pGLO LB/amp/). Out of the two genetically transformed bacteria, only the bacteria containing arabinose sugar (+pGLO LB/amp/ara) initiated expression of the GFP (Green Fluorescent Protein) gene, causing the bacterial...
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...i bought my toshiba netbook last year and it came with a windows 7 starter. two weeks ago i saw this at my system properties " Get more features with a new edition of Windows 7" and clicked it. and after a while it became windows 7 ultimate. but yesterday, a pop-up appeared on my taskbar saying that my windows is not genuine. now i cant change my background wallpaper and there is a message in the lower right of the screen saying "windows 7 ultimate, build 7601, this copy of windows is not genuine". i ran the diagnostic and this is the report. PLEASE HELP ME!! Diagnostic Report (1.9.0027.0): ----------------------------------------- Windows Validation Data--> Validation Code: 50 Cached Online Validation Code: 0xc004c4a2 Windows Product Key: *****-*****-YG69F-9M66D-PMJBM Windows Product Key Hash: /kehptF9HHVxM5d8dUnqgcfndXw= Windows Product ID: 00426-OEM-8992662-00497 Windows Product ID Type: 2 Windows License Type: OEM SLP Windows OS version: 6.1.7601.2.00010100.1.0.001 ID: {50A2E917-6A96-4AE0-ACB0-F354288D364B}(3) Is Admin: Yes TestCab: 0x0 LegitcheckControl ActiveX: N/A, hr = 0x80070002 Signed By: N/A, hr = 0x80070002 Product Name: Windows 7 Ultimate Architecture: 0x00000000 Build lab: 7601.win7sp1_gdr.111118-2330 TTS Error: Validation Diagnostic: Resolution Status: N/A Vista WgaER Data--> ThreatID(s): N/A, hr = 0x80070002 Version: N/A, hr = 0x80070002 Windows XP Notifications Data--> Cached Result: N/A, hr = 0x80070002 File Exists:...
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...each unit representing tenfold change in H+ ion concentration. The idea of the pH scale was devised by a Danish biochemist and part-time beer brewer named Soren Sorensen in 1909. He was searching for a convenient means of checking the acidity of his alcoholic product to prevent its spoilage by bacterial action. (Human Anatomy and Physiology; Seventh Edition ) Acids are proton donors while bases are proton acceptors. Referring to the pH scale numbers zero to fourteen, stomach acid and lemon juice are examples of strong acids having a pH of one and two while ammonia is an example of a base having a pH of 12. Milk and water are considered neutral because they are pretty even in the middle of the pH scale with a six and seven. (A&P I Lab Manual) pH is important to the body because thousands, maybe even millions of chemical reactions occur within the body. For you to live, all these reactions must be somewhat successful. For this to happen certain variables...
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