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Organic Foods Lab

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Introduction
In today's world everything is somewhat modified, for example, plants are always being modified to fulfill better the humanity necessity and to resist the environment. Even though the greater part of the food that we consume is genetic modified (GMOs), still some foods that didn’t go through genetic modification (organic food). So a lab was designed with the objective to identify if the food consumed was GMO or organic.
The 35s promoter is a common characteristic that only a genetic modify plant have in its genetic material. If the plant’s DNA is isolated and experience a series of polymerase chain reaction (PCR), it is possible to identify the existence of 35s promoter by looking at a band around 162bp in an electrophoresis gel. …show more content…
Then 100 µL of Edwards buffer (dH2O, 1M Tris pH 8.0, 5M NaCl, 0.5M EDTA, 10% SDS) was added to each tube, and then the sample was grinded and well mixed. Another 900 µL of Edwards buffer was added to the mixture and vortexed to ensure the DNA extraction. The three tubes were then boiled for approximately 5 minutes, when the time was over the samples were centrifuged for 2 minutes at 12,000rpms. Next for each sample, 350 µL of the supernatant produced after the centrifugation was transferred to a clean tube and well mixed with 400 µL of isopropanol. The samples were allowed to rest for 3 minutes and then centrifuged once again for 5 minutes at 12,000 rpms. After centrifugation, the supernatant was removed, and the pellets were air-dried for 10 minutes. Finally, in each tube 100 µL of TE/RNase A buffer was added and then centrifuged for 1 minute at 12,000 rpms. * To preserve the samples it was necessary to store them in …show more content…
When all the beads were dissolved in each tube 2.5 µL of DNA sample was loaded to its respective vials. Finally, the other three premade PCR tubes was used and in each of them received 22.5 µL of the tubulin primer. When the beads were completely dissolved, each tube received 2.5 µL of each respective DNA sample.
With all samples in hand it was time to place all of them in the thermocycler. The machine ran for a total of 36 cycles at different conditions, fists at 96oC for 10 seconds, then at 94oC for 10 seconds, 55oC for 10 seconds, 72oC for 15 seconds, and 72oC for 1

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