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Submitted By amylily
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Title: The effect of the concentration of hydrogen peroxide on the rate of reaction of catalase from potato
Focus question: How will the concentration of the substrate hydrogen peroxide affects the rate of reaction of the enzyme catalase.
Apparatus/Materials: Goggles, Lab coat, Potato, Core, 100mL beaker, Hose and stopper, Different concentrations of hydrogen peroxide (3.0%, 6.0% and 9.0%), Gas collecting tube
Variables:
Dependent variable: (1) rate of oxygen production
Independent variable: (1) Concentrations of hydrogen peroxide (3.0%, 6.0% and 9.0%)

Controlled variable: (1) The size and mass of the potato slices (2) The amount of time each experiment was left (3) The volume of hydrogen peroxide placed in the cylinder (4) The size of the test tubes (5) Temperature was left constant at room temperature (optimum temperature for catalase)
Procedure:
1. The lab coat and googles were put on 2. A potato was sliced into two equal pieces using a core 3. The potato slices was placed into a test tube 4. The gas collection tube was completely filled with water and placed into a 100mL beaker which was also filled with water. 5. The hose in the ‘stopper and hose’ was placed in the gas collection tube 6. 10 mL of 3% hydrogen peroxide was added and the stopper was immediately attached to the test tube as seen in the diagram below 7. The amount of oxygen produced was measured every minute for five minutes 8. Observations and results were recorded 9. Steps 2-8 was repeated using twice using 6% hydrogen peroxide and 9% hydrogen peroxide respectively. 10. A graph of oxygen produced versus time was plotted

Observations
Qualitative
3% H2O2 | 6% H2O2 | 9% H2O2 | Gas collecting tube contained the least gas | Gas collecting tube contained the moderate gas | Gas collecting tube contained the

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