PASIG CITY SCIENCE HIGH SCHOOL
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RESEARCH PLAN

I - Proposed Research Topic: Anti-paw edema property of Lupeol extracted from (Capsicum annuum var. longum) Siling Haba fruit against Streptococcus pyogenes and Mycobacterium tuberculosis. II - Proponents:

Ariane Joy C. Listanco
Alyssa Mae C. Eguia
Beatrice Ann P. Sablay
Ashley L. Quebrado
Alexis R. Redondo
Raffy A. Wamar

III - Background of the Study:

In this study, we sought to know the effectiveness of the anti-paw edema property of Lupeol on
Streptococcus pyogenes and Mycobacterium tuberculosis. We will investigate the effects of
Lupeol on the organisms in different concentration. There will be three replicates for every trial in order to have an accurate answer. Nonetheless, the extraction of the Lupeol from capsicum is to be done by laboratories available. The researchers were the one that will extract the Lupeol from Siling Haba with the aid of acid that came from an apple. The researchers want to expand the study and employments of Siling Haba for cooking purposes as well as for its therapeutic employments. Siling mahaba, siling haba, (long chili), siling pangsigang (chili for sinigang), siling
Tagalog (Tagalog chili), and sometimes called finger chili, is one of two common kinds of native chili found in the Philippines, the other being siling labuyo. The siling mahaba fruit grows to

between five and seven centimeters long, and is bright light green in color. While of moderate spiciness, it is much milder and less hot than siling labuyo.
It is an ingredient commonly used in Philippine cuisine, spicing up dishes like sinigang, dinuguan, pinangat, kilawin, paksiw, and sisig. This type of specie is not commonly used in research studies. So the researchers have decided to use this variable to expand the study for this certain species.

In this study, we tried to know the adequacy of the opposition to paw edema property of Lupeol on Streptococcus pyogenes and Mycobacterium tuberculosis. We will examine the impacts of
Lupeol on the organic entities in distinctive focus. There will be three repeats for every trial keeping in mind the end goal to have an exact answer. Nonetheless, the extraction of the Lupeol from capsicum is to be carried out by research centers accessible. The analysts were the particular case that will extricate the Lupeol from Siling Haba with the help of corrosive that originated from an apple. The analysts need to grow the study and employments of Siling Haba for cooking purposes as well as for its therapeutic employments.

Siling mahaba, siling haba, (long stew), siling pangsigang (bean stew for sinigang), siling
(Tagalog stew), and once in a while called finger bean stew, is one of two regular sorts of local bean stew found in the Philippines, the other being siling labuyo. The siling mahaba organic product develops to somewhere around five and seven centimeters in length, and is splendid light green in shading. While of moderate fieriness, it is much milder and less hot than siling labuyo. It is a fixing normally utilized as a part of Philippine cooking, spicing up dishes like sinigang, dinuguan, pinangat, kilawin, paksiw, and sisig. This kind of specie is not generally utilized as a part of examination studies. So the analysts have chosen to utilize this variable to grow the study for this certain species.
IV - Research Objectives:


This study aims to determine the Anti-paw edema property of Lupeol extracted from
Capsicum annuum var. longum (Green Chili) fruit against Streptococcus pyogenes and
Mycobacterium tuberculosis.



The goal of this study is to demonstrate its effectiveness against Streptococcus pyogenes and Mycobacterium tuberculosis.



To extract the Lupeol from Capsicum annuum var. longum) (Green Chili) fruit.



It aims to help the society by giving uses of the things that was not commonly used.



To contribute to the field of medicine.

V - Materials to be used
 Petri dish
 Streptococcus pyogenes and Mycobacterium tuberculosis organism.
 Test tubes
 Test tube rack
 Dropper
 Gloves
 Face mask
 Green Chili depending on the number you want to use
 Laboratory mice
 Syringe

VI – Methodology

A. Methodological Framework
Anti-paw edema property of Lupeol extracted from Capsicum annuum var. longum (green chili) fruit against Streptococcus pyogenes and Mycobacterium tuberculosis.

Culturing of Streptococcus pyogenes and
Mycobacterium tuberculosis.

Conduct of the experiment.

Keen observation.
Infecting the mice through exposing by Streptococcus pyogenes and Mycobacterium tuberculosis.

Extraction of Lupeol from Capsicum annuum var. longum (green chili).

Injecting the extracted Lupeol to the mice.

B. Research Methodology

Data collection.
Analyzation of the data. In conducting this experiment, the researchers will require to utilize several materials.
Two microorganisms which are staphylococcus aureus and salmonella typhimurium will require to at least have three trials in the two microorganisms respectively depending on how many level of concentration of the Lupeol to be applied. There is also a need for the extraction of
Lupeol coming from Capsicum annuum var. longum (green chili) for the direct application in the microorganisms. C. Research Procedure

Culturing
The researchers must choose at least two microorganisms to culture in three different trials.
With the microorganisms selected, identify the type of culture media needed. Decide whether it will be tested on organisms such as mice and etc.
Extraction
With the chosen laboratory support, the researchers will be required to have three test tubes containing the different level of concentration of Lupeol extracted from Capsicum annuum var. longum (green chili). In extracting the Lupeol the procedure for working on it must be chosen by the researchers.
Application
Once the culture and extraction has been made, the researchers will now conduct the experiment by exposing the microorganisms to laboratory mice and injecting it with the extracted Lupeol.
Observation
After conducting the experiment, there will be needing a keen observation to the two microorganisms to see the effect of the anti-paw edema property of Lupeol to the laboratory mice. Data Collection

After determining the effect of the anti-paw edema property of Lupeol from Capsicum annuum var. longum (green chili) on mice that being infected by those selected microorganisms, the researchers must gather all the data needed for the analyzation of the study. Lastly, an accurate outcome should be presented and the result should be validated.

VII - Background Literature/Studies
Capsaicin
All chili peppers, including green chilis, contain capsaicin, the compound that is responsible for the spicy sensation associated with eating a hot pepper. The spicier the pepper is, the more capsaicin it contains. The Scoville Heat Unit scale, which measures capsaicin content from 0 to
15,000,000 Scoville Heat Units, or SHUs, of pure capsaicin, places green chilis between 500 and 2,500 SHUs. The American Institute for Cancer Research reports that eating rich capsaicin sources like green chili peppers may help inhibit the growth of cancer cells, though more research is needed.
Green chilis are immature chili peppers, most often Pasilla, Anaheim or Poblano peppers that have been harvested before fully ripening. Not as spicy as red chili peppers, they are available raw, canned or pickled and are a common addition to traditional Hispanic dishes ranging from soups to snacks. Green chilis are low in calories, virtually fat-free and rich in nutrients that can enhance your health, including vitamins A, C and K and the phytonutrient capsaicin. If you have a chronic digestive disorder like irritable bowel syndrome or heartburn, however, spicy foods like chilis may exacerbate the symptoms.

Lupeol has a complex pharmacology, displaying antiprotozoal, antimicrobial, antiinflammatory, antitumor and chemopreventive properties.
Animal models suggest lupeol may act as an anti-inflammatory agent. A 1998 study found lupeol to decrease paw swelling in rats by 39%, compared to 35% for the standardized control compound indomethacin.
One study has also found some activity as a Dipeptidyl peptidase-4 inhibitor and prolyl oligopeptidase inhibitor at high concentrations (in the millimolar range).
It is an effective inhibitor in laboratory models of prostate and skin cancers.
As an anti-inflammatory agent, lupeol functions primarily on the interleukin system. Lupeol to decreases IL-4 (interleukin 4) production by T-helper type 2 cells.