THE PASSAGE OF SALYCILATE INTO YEAST CELLS

AIM: Aim of the experiment is to investigate and analyse the effect of pH on the transfer of salicylate across membranes.
INTRODUCTION: As Schedule
METHOD: As Schedule

RESULTS:

Test Tube number | Absorbance | 1 | 0 | 2 | 0.171 | 3 | 0.337 | 4 | 0.496 | 5 | 0.598 | 6 | 0.801 | 7 | 1.598 | 8a | 0.46 | 9a | 0.629 | 10a | 0.701 | 11a | 0.899 | 8b | 0.704 | 9b | 0.793 | 10b | 0.830 | 11b | 0.877 |

1. 1.0 x (PCV/100)X0.9= Volume of yeast
The PCV= 18%
:. 1.0x (18/100)x 0.9 = VY
Vy= 0.162 2. Total volume- yeast volume= volume of supernatant
:. 3.0ml- 0.162 = 2.838

3. Concentration of salicylate | Sal.concentration | V.S x C.S | Amount of sal. In yeast cells | Conc of sal. Inside yeast | 1 | 0 | 0 | 0 | 0 | 2 | 0.1 | 0.2838 | 1.7162 | 10.59 | 3 | 0.2 | 0.5876 | 1.4324 | 8.84 | 4 | 0.3 | 0.8514 | 1.1486 | 7.09 | 4 | 0.4 | 1.1352 | 0.8648 | 5.34 | 6 | 0.5 | 1.419 | 0.581 | 3.59 | 7 | 1.0 | 2.838 | -0.838 | 5.17 |

4. Volume of supernatant x concentration of salicylate
:.
0 x2.838 = 0 0.1 x 0.2838 = 0.2838 0.2 x 0.2383 =0.5876 0.3 x 0.2383 = 0.8514 0.4 x 0.2383 = 1.1352 0.5 x 0.2383 = 1.419 0.6 x 0.2383 = 2.838 5. Amount of salicylate in yeast cells= 2mg-amount of salicylate in supernatant
2mg.
( Please refer to table 2)

6. Concentration of salicylate inside yeast = amount of sal./ V.Y
(Please refer to table 2).

7. PH = PKa + log(salt/acid) = pKa+ log{A-/AH}

:.
2.9 =3.0 + log{A-/AH} PkA 3.0 for salicylate
2.9 – 3.0 =-0.1=