...7.4f Tumour markers In health, cell development, growth and death are regulated by complex chemical interactions. In cancer cells, these interactions are disturbed resulting in uncontrolled cell division, tumour development and malignancy. In these cells, abnormal types and levels of substances are produced and secreted into body fluids such as blood. Here, the tumour markers can be quantified by laboratory assay techniques. The ideal tumour marker should * be highly specific and sensitive to a type of cancer * directly reflect the progression of the cancer through the measured level * have a short half life to rapidly reflect treatment * discriminate between benign and metastatic growths * have low levels in the healthy population * have good assay sensitivity Tumour markers that fit these criteria have an important role to play in the screening, diagnosis and monitoring of cancers. These can be measured qualitatively by histological techniques or quantitatively in biochemistry labs by serological methods. Screening No tumour marker is specific or sensitive enough to be used as a definitive screening test for cancer. However, certain tumour markers have proved successful in screening for cancers in high risk populations. This is the case of prostate specific antigen or (PSA) and prostate cancer in middle to late aged men. In China α-fetoprotein (AFP) measurement are used for screening of hepatocellular carcinoma and in the UK faecal occult blood...
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...It can scavenge 50-75% of reactive radicals such as OH● [55]. Reactive oxygen species induced protein modifications can result in unfolding or alteration of protein structure [56]. Protein reversible modification such as S-glutathionylation, S-nitrosation and methionine sulfoxidation may have dual role of protection from irreversible oxidation and alteration of protein function[57-59]. Protein carbonylation is an irreversible and non-enzymatic reaction. Irreversible protein modification can lead to inactivation of various proteins, loss of their structure and function and could have detrimental effects on the cell. Therefore, protein carbonyl content is the most commonly used marker of protein oxidation[60]. Exposure of gas-phase of cigarette smoke modified human plasma proteins which produces carbonylated proteins with loss of sulfhydryl groups [61]. Recently, Nagai K et reported that the concentration of oxidized proteins in broncho-alveolar lavage fluids were significantly increased in asymptomatic smoker as compared to non-smokers...
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...Describe the structure-function relationships of human IgG and how might such an understanding influence the choice of IgG subclass in potential antibody-based therapies? Our body is under constant attack whether from external with viruses, bacteria and parasites or from the internal; with the development of tumour cells in cancer. We have a number of ways to prevent organisms from entering our body and surviving. This includes our skin, mucous membranes and the low pH of our stomach acids. However, if an organisms pass this barriers, the body have specific self defence mechanisms to act on these invasions. Humans have an intricate network of highly specialised cells known collectively as leukocytes or more commonly as white blood cells. Each cells is highly specialised and plays a critical role in the human response. One example is the HIV virus which infects and kills one particular type of immune cell which cause devastating consequences to the entire immune system. When the human body immune system invasion can lead to severe harm, the importance of understanding the structure and functions of this system may be seen as a vital role in combating the effect of these foreign substances. This essay will give some insight into the human IgG class structures and functions which is highly important in human defence system studies and show how these understanding can help in the development of potential antibody-based therapies. Antibodies is a group of protein which is involved...
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...There are no sources in the current document.The purpose of this essay is to explain the role of cytokines in depression. Over the last few decades there have been many controversial hypotheses put forward to explain the observation of depressive symptoms in people, who have a physical injury or are under psychological stress (Schiepers, et al., 2008). Research shows that most controversy was over how the messages from the immune system were interpreted or translated into being a signal to the central nervous system (CNS). While most research agreed that there was a correlation in data between cytokines and depressive symptoms, they did not believe this indicated a causal link (Anisman, 2002). I have chosen to focus one of the most popular theories, the “cytokine theory of depression”, as I believe the research shows that it has a causal link between inflammation, cytokine activity and depressive symptoms (Roque, et al., 2009). This hypothesis recognises and explains the highly integrated processes and mechanisms that a person or animal goes through, from the initial stressor to displaying the depressive symptoms, termed as “sickness behaviour” (Dantzer, 2006). “Sickness Behaviour” is the term given to the symptoms caused by high levels of pro-inflammatory cytokines in the periphery of the body; these symptoms overlap with the general characteristics of depression and include; decreased appetite, increased desire to sleep, cognitive dysfunction, social withdrawal and decreased...
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...Vol 450 | 20/27 December 2007 | doi:10.1038/nature06385 LETTERS Isolation of rare circulating tumour cells in cancer patients by microchip technology Sunitha Nagrath1*, Lecia V. Sequist2*, Shyamala Maheswaran2, Daphne W. Bell2{, Daniel Irimia1, Lindsey Ulkus2, Matthew R. Smith2, Eunice L. Kwak2, Subba Digumarthy2, Alona Muzikansky2, Paula Ryan2, Ulysses J. Balis1{, Ronald G. Tompkins1, Daniel A. Haber2 & Mehmet Toner1 Viable tumour-derived epithelial cells (circulating tumour cells or CTCs) have been identified in peripheral blood from cancer patients and are probably the origin of intractable metastatic disease1–4. Although extremely rare, CTCs represent a potential alternative to invasive biopsies as a source of tumour tissue for the detection, characterization and monitoring of non-haematologic cancers5–8. The ability to identify, isolate, propagate and molecularly characterize CTC subpopulations could further the discovery of cancer stem cell biomarkers and expand the understanding of the biology of metastasis. Current strategies for isolating CTCs are limited to complex analytic approaches that generate very low yield and purity9. Here we describe the development of a unique microfluidic platform (the ‘CTC-chip’) capable of efficient and selective separation of viable CTCs from peripheral whole blood samples, mediated by the interaction of target CTCs with antibody (EpCAM)-coated microposts under precisely controlled laminar flow conditions, and without requisite...
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...Name: Student Number: UNIVERSITY OF TORONTO Faculty of Arts and Sciences APRIL 2011 EXAMINATIONS LMP301H1S DURATION: 2 HOURS Examination Aids: Non-programmable calculators are allowed Version A SECTION I – MULTIPLE CHOICE (88 marks) The 44 questions are of equal value (2 marks). Select the most correct answer for each question. 1. Which of the following statements is TRUE regarding lipid biomarkers? i. ii. iii. iv. A. B. C. D. E. Total Cholesterol to HDL-C ratio is more sensitive for heart disease than cholesterol alone Elevated triglycerides are a defining criterion in metabolic syndrome Increase in lipoprotein(a) reflects accumulation of the small-dense form of LDL particles Measurement of Apo-A1 levels is useful in patients with metabolic syndrome at high risk i and ii i and iii iii and iv iv only All of the above (i, ii, iii and iv) 2. Which of the following statements is FALSE regarding regulation of body water? A. B. C. D. E. Receptors in the hypothalamus respond to increased osmolality and stimulate thirst As the effective circulating volume increases, aldosterone secretion is suppressed Congestive heart failure may arise from volume overload of the circulatory system Anti-diuretic hormone stimulates the Na+/K+ transporter to retain sodium and water Angiotensin II stimulates vasoconstriction and sodium resorption in the kidney Page 1 of 15 Name: Student Number: 3. Given the following test results: sodium potassium chloride bicarbonate glucose...
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...Paraben Paradox Parabens are preservatives used in a variety of personal care, cosmetic, pharmaceutical, food products. Studies have confirmed the ubiquitous presence of parabens, with levels detected in wastewater, rivers, soil and house dust. Parabens have also been detected in human tissues and bodily fluids. Parabens are popular preservatives because of their low toxicity and cost, their broad inertness, and their worldwide regulatory acceptance (Soni et al. 2005). Methyl paraben (MP) and propyl paraben (PP) are two of the most used parabens (Andersen 2008). Parabens easily penetrate the skin. The European Commission on Endocrine Disruption has listed parabens as Category 1 priority substances, based on evidence that they interfere with hormone function. Parabens can mimic estrogen, the primary female sex hormone. They have been detected in human breast cancer tissues, suggesting a possible association between parabens in cosmetics and cancer. It has been estimated that women are exposed to 50 mg per day of parabens from cosmetics. However, studies investigating the health effects of parabens are conflicting. There are no restrictions on the use of parabens in cosmetics in Canada, whereas International regulations are stronger. The European Union restricts the concentration of parabens in cosmetics. Parabens are typically...
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...Benefits of Immunotherapy from advances in immunology and recombinant DNA technology. Immunotherapy is a form of medical treatment intended to stimulate or restore the ability of the immune system to fight infection and disease. This can be by inducing, enhancing, or suppressing an immune response. Immunotherapies designed to elicit or amplify an immune response are classified as activation immunotherapies, while those that reduce or suppress immune response are suppression immunotherapies. Active immunotherapy has been effective against agents that normally cause acute self-limiting infectious disease. However, a more effective immunotherapy for chronic infectious diseases or cancer requires the use of appropriate target antigens; the optimization of the interaction between the antigenic peptide, the antigen-presenting cells (APC) and the T cell; and the simultaneous blockage of the negative regulatory mechanisms that impede immunotherapeutic effect. Furthermore, passive immunotherapy using monoclonal antibodies and receptor FC-fusion proteins has come of age and has shown great clinical success. Several monoclonal antibodies, including unmodified antibodies and antibodies armed with toxins or radionuclides, have been approved to prevent allograft rejection or to treat autoimmune diseases and cancer. Several others are in clinical trials. Preventive vaccines have been successful against infectious agents such as viruses, which cause self-limiting diseases that are normally...
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...Hindawi Publishing Corporation Obstetrics and Gynecology International Volume 2013, Article ID 173184, 11 pages http://dx.doi.org/10.1155/2013/173184 Review Article Uterine Fibroids: Pathogenesis and Interactions with Endometrium and Endomyometrial Junction Andrea Ciavattini,1 Jacopo Di Giuseppe,1 Piergiorgio Stortoni,1 Nina Montik,1 Stefano R. Giannubilo,1 Pietro Litta,2 Md. Soriful Islam,3 Andrea L. Tranquilli,1 Fernando M. Reis,4 and Pasquapina Ciarmela3 1 Woman’s Health Sciences Department, Faculty of Medicine, Polytechnic University of Marche, Via Corridoni 11, 60123 Ancona, Italy Department of Gynaecological Sciences and Human Reproduction, University of Padova, Via Giustiniani 3, 35128 Padova, Italy 3 Department of Experimental and Clinical Medicine, Faculty of Medicine, Polytechnic University of Marche, Via Tronto 10/a, 60126 Ancona, Italy 4 Department of Obstetrics and Gynecology, Federal University of Minas Gerais and National Institute of Hormones and Women’s Health, 30130-100 Belo Horizonte, MG, Brazil 2 Correspondence should be addressed to Andrea Ciavattini; ciavattini.a@libero.it Received 28 February 2013; Revised 10 June 2013; Accepted 13 August 2013 Academic Editor: Hilary Critchley Copyright © 2013 Andrea Ciavattini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Uterine leiomyomas (fibroids...
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...Email: nm@biotecnol.com Abstract Tribodies are multifunctional recombinant antibody derivatives, which utilize the natural in vivo heterodimerization of the heavy chain (Fd fragment) and light chain (L) of a Fab fragment, to form a scaffold, upon which additional functions can be incorporated, such as additional binders - e.g. scFv binding domains. Each chain can be extended preferably at the C-terminus with an additional scFv binder. The chains are co-produced in mammalian cells, where the host-cell BiP chaperone drives the formation of the heavy chain-light chain heterodimer (Fd:L) - this reaction does not appear to be inhibited by the chain extensions, and leads to a very specific heterodimerization, using molecules abundantly present in serum (non-immunogenic) These heterodimers are stable, with each of the binders retaining their specific affinities, with the bivalent tribody having higher affinity, and higher activatation of T-cell proliferation and cytotoxicity in vivo. This design allows easy engineering of multispecificity in a single molecule, e.g. bispecific antibodies bivalent for the target and monovalent for effector activation (e.g. for T-cell activation), or trispecific antibodies pur sang. 1 Introduction ............................................................................................................................................................................................... 2 2 Tribody Structure, Production and Stability .......................
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... April 2012 Table of Contents 1.0 The Immune System 3 2.0 Antibodies 6 3.0 Avian Antibodies 7 4.0 Why IGY? 8 5 .0 Polyclonal Versus Monoclonal Antibodies………………………………………………………..9 6.0 Purpose of IGY Inc. – Antibody Extraction 10 7.0 IGY for Use in Humans ………………..………………………………………………………….11 8.0 References 15 IGY Immune Technologies & Life Sciences Inc. 1.0 The Immune System The human immune system is comprised of a complex network of lymphoid organs and highly specialized cells that protect the body against infection by recognizing and killing pathogens. This immune system includes lymphatic organs and vessels, lymph nodes, white blood cells, specialized cells and serum factors. The immune system utilizes both the lymphatic vessels and the blood circulatory system to transport white blood cells called lymphocytes through out the body (1). The immune system can be classified as an innate or adaptive system. As a first line of defense against pathogens, we depend on innate immunity. Innate immunity is nonspecific and includes physical barriers such as skin, mucous membranes and secretions with antimicrobial activity including tears and mucous. Pathogens that cross this line of defense are often destroyed by phagocytes which are white blood cells that engulf and digest cellular debris and foreign agents by the process known as phagocytosis. However, innate immunity is not sufficient to ward off all...
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...Alternative Cancer Therapies Table of Contents 714-X ABM Mushroom AHCC Aloe Vera Anticoagulants Antineoplastons Antioxidants Anvirzel Artemisinin Asparagus Berries Boluses Bovine Cartilage Cancell Cansema Carnivora Alternative Cancer Therapies Page 1 Updated 05/17/11 Bookmark this page...as we learn of more therapies throug Please report any broken links by contacting info@mnwelldir.org Perhaps we should call these "unproven therapies" since many of them are on the American Cancer Society's infamous black list. Simply because something is "unproven" does not mean that it has been "disproven." And if a therapy fits the following— 1. It works. Castor Oil Packs Cayenne Pepper Chaparral Chinese Bitter Melon Chiropractic Clodronate Coley's Toxins Contortrostatin C-Statin D-limonene DMSO Electrolyzed Water Ellagic Acid Enzyme Therapy Escharotics Essential Oils 2. It's inexpensive. 3. Few, if any, negative side effects. 4. It's not patentable. —odds are it will stay on the black list because no one is going to spend a dime to prove its effectiveness. Medicine is a business. Cancer is a business. The FDA is running a protection racket, protecting drug companies and the AMA from anyone with an inexpensive and effective treatment for money making diseases. The following therapies are not guaranteed to work, at least by us. They are presented to you for information purposes only. For many, their effectiveness has been shown in limited clinical trials, but each one, by itself, is not...
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...-[ \ UNIVERSITY OF CAPE COAST SCHOOL OF NURSING MSC. ADVANCED NURSING PRACTICE COURSE: ADVANCED CLINICAL PRACTICUM I AND II IN SPECIALTY AREA COURSE CODE: NUR 822S and NUR 829S PATIENT / FAMILY CASE STUDY (A NURSING PROCESS APPROACH) ON A CLIENT WITH GESTATIONAL TROPHOBLASTIC NEOPLASM BY: CHARLOTTE LAMPTEY SN/ADN/15/0030 AUGUST, 2016 CONTENTS * PREFACE * ACKNOWLEDGEMENT * INTRODUCTION CHAPTER ONE: OVERVIEW OF CLIENT SITUATION I. Literature review of gestational trophoblastic neoplasm CHAPTER TWO: COMPREHENSIVE HOLISTIC ASSESSMENT OF PATIENT/FAMILY I. Patient’s medical and personal history including review of the systems II.Physical examination III.Diagnostic evaluation IV.Nutritional assessment V. Psychosocial history VI. Patient developmental assessment VII.Spiritual assessment VIII.Quality of life assessment IX.Admission of patient CHAPTER THREE: ANALYSIS OF DATA CHAPTER FOUR: COLLABORATIVE PLAN OF CARE I. Presumptive medical diagnosis II.Nursing diagnosis III.Evidence-based interventions IV.Additional diagnostic procedures warranted but not done Medication to be ordered CHAPTER FIVE: DISCHARGE PLAN I. Community service and resource needed II.Client education plan III.Plans for follow-up of care CHAPTER SIX: EVALUATION PLAN Termination of care * SUMMARY * CONCLUSION * REFERENCE PREFACE ...
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...Alzheimer's disease is the most common cause of dementia. Research advances have enabled detailed understanding of the molecular pathogenesis of the hallmarks of the disease-ie, plaques, composed of amyloid β (Aβ), and tangles, composed of hyperphosphorylated tau. However, as our knowledge increases so does our appreciation for the pathogenic complexity of the disorder. Familial Alzheimer's disease is a very rare autosomal dominant disease with early onset, caused by mutations in the amyloid precursor protein and presenilin genes, both linked to Aβ metabolism. By contrast with familial disease, sporadic Alzheimer's disease is very common with more than 15 million people affected worldwide. The cause of the sporadic form of the disease is unknown, probably because the disease is heterogeneous, caused by ageing in concert with a complex interaction of both genetic and environmental risk factors. This seminar reviews the key aspects of the disease, including epidemiology, genetics, pathogenesis, diagnosis, and treatment, as well as recent developments and controversies. 100 years ago, Alois Alzheimer gave a lecture at a congress in Tubingen, Germany, on the first case of the disease that Kraepelin some years later named Alzheimer's disease.1 In this single case. Alzheimer described typical clinical characteristics with memory disturbances and instrumental signs, and the neuropathological picture with miliary bodies (plaques) and dense bundles of fibrils (tangles), which we today...
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...I. 진단혈액 진단혈액 수련항목 (1) : 혈액학적검사 기본 술기 표준수련기간 : 1주 수련내용 : ◆ 용어정의 : • 혈액학적검사 : 혈액세포와 응고에 관련된 일련의 검사를 의미한다. 혈구의 체내분포, 구조, 기능에 관련된 검사, 골수에 분포하는 전구세포에 관한 검사, 혈구에 영향을 끼칠 수 있는 혈장 인자에 관한 검사 및 유전자 이상에 관련된 검사 등을 포괄적으로 포함한다. • 망상적혈구수 : 적혈구 성숙 단계 중 정염색성 적아구(orthochromatophilic normoblast) 바로 다음 단계의 세포로 핵이 빠져나간 직후부터를 의미한다. 미토콘드리아, 중심소체(centriole), 리보솜 등을 함유하고 있으며 말초혈액에서 24-48시간의 성숙과정을 거쳐서 성숙한 적혈구로 된다 (Ref. Williams 16th p373-374) ◆ 숙지할 필수 지식 : • 혈액학 검사에 사용되는 검체와 항응고제의 작용기전 및 종류 • 모세관 혈액의 채취 방법과 용도, 채취 시 주의점 및 정맥혈과의 차이점 • 적혈구침강계수(ESR) 검사의 원리 ◆ 습득할 필수 술기 : • Neubauer chamber의 사용 • 미량법(micromethod)를 이용한 헤마토크리트의 측정 • 수기법을 이용한 망상적혈구수 검사 ◆ 국내외 장비 및 시약 현황 : 해당없음 ◆ 추천되는 참고자료 : • 대한혈액학회. 혈액학, 2006. • 대한진단검사의학회 편, 진단검사의학 제 3판, 2001. • Henry, JB. Clinical Diagnosis and Management by Laboratory Methods, 24th ed. 2006. 보고서 제출 일자 : 200 년 월 일 평가자 : 지도전문의 인 (일자 : 200 년 월 일) 과장 인 (일자 : 200 년 월 일) 수련위원 인 (일자 : 200 년 월 일) 진단혈액 수련항목 (2) : 자동 혈구계산기 표준수련기간 : 2주 수련내용 : ◆ 용어정의 : • 헤마토크릿(Hct) : 혈액 전체 부피에 대한 적혈구 부피의 비율, 단위는 % 또는 L/L • 평균적혈구용적(MCV) : 적혈구의 평균 용적, 단위는 fL, • MCV = Hct (L/L) X 1,000/RBC count (X1012/L) • 평균적혈구혈색소(MCH) : 적혈구 한 개당 혈색소 양, 단위는 pg, • MCH = hemoglobin (g/L)/RBC count (X1012/L) • 평균적혈구혈색소농도(MCHC) : 적혈구 한 개당 평균 혈색소 농도, • 단위는 g/L, MCHC = hemoglobin (g/L)/Hct (L/L) • 적혈구분포지수(RDW)...
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