S.NO TOPICS PAGE#
01 Object, Introduction, Method 01. 1, 2
02 Method 02, Method 03. 3
03 Method 04, Method 05. 4, 5
04 References. 6,7

OBJECT:
To determine the presence of Vitamin C by number of many methods.
INTRODUCTION:
Vitamin C is powerful antioxidant and it is also known as ascorbic acid and found mostly in fruits and vegetables it helps the body form and maintain connective tissue including bone, blood vessel and skin. Most experts recommended that Vitamin C from a diet high in fruits and vegetables rather than taking supplements. Fresh squeezed orange juice or fresh frozen concentrate is better pick than ready to drink orange juice. The fresh juice contain more active Vitamin C. Drink fresh frozen juice within one week after reconstituting …show more content…
METHOD 03:
SPECTROPHOTOMETRIC DETERMINATION:
A shimadzu spectrophotometer (model UV-1601) with a pair of 1 cm quartz cell was used. For this reagent required 5% of metaphosphoric acid , 10%acetic acid, 15 gms of solid metaphosphoric acid were dissolved in a mixture of 40 ml of glacial acetic acid and 450 ml of distilled water in a 500 ml volumetric flask this solution was filtered and collected.

10% THIOUREA SOLUTION, 2,4 DINITROPHENYL-HYDRAZINE SOLUTION,85%SULPHURIC …show more content…
Waste material should be washed properly or solid waste throw in the bin which cause pollution and hazardous effect in the whole environment.
TITRATION OF VITAMIN C :
STEP 01:
STANDARD SOLUTION FOR ASCORBIC ACID:
Take 25 ml of Vitamin C Plus 10 drops of 1% starch solution then tirate the whole solution until light blue color is observed after this record the final and initial concentration of iodine is note and you will get the concentration of vitamin C after doing this titration step 01.
STEP 02:
FRUIT JUICE SAMPLES ARE BLACK CURRANT, ORANGE AND MANGO:
Take 1.25 ml of juice and add 10 drop of 1% starch solution after this titrate the solution until end point reaches and giv light blue color which indicates the presence of vitamin C in the solution this titration is repeated 2 to 3 times for average and accurate result now record the intial and final volume of iodine solution is used in performing.