. Peroxidase (PO) Peroxidases is a multipurpose stress sensitive enzyme (Thakker et al., 2013) that catalyses the oxidation of variety of substrates, mainly hydrogen peroxide (H2O2). The H2O2-dependent reaction catalyzed by Peroxidases results into the condensation of phenolics to lignins (Thakker et al., 2013), thereby removing the toxic H2O2 from tissues (Lebeda et al., 1999). The key function of PO is lignin biosynthesis (Bruce & West, 1989) in plants. Lignins add strength to the cell wall creating
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3.2.4 Investigating Enzyme Action The effect of catalase concentration on reaction rate with Hydrogen Peroxide 3 March 2016 Nazka Nurbyek Abstract The purpose for this reaction was to study the effect of catalase concentration on reaction rate with Hydrogen Peroxide. Baker’s yeast in the amounts of 0.1 g, 0.2 g, 0.3 g, 0.4 g, and 0.5 g were used as a source of catalase. Yeast was suspended in 1.5% Hydrogen Peroxide solution (H2O2) and reaction rate with catalase was measured using a gas pressure
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The pathogenicity of entomopathogenic fungi, Beauveria bassiana, Metarhizium anisopliae and Verticillium lecanii, was evaluated against the adult of vine mealybug ,Planococcus ficus (Signoret) under laboratory conditions by dipping bioassays. The results showed that the virulence of B. bassiana was higher than the virulence of M.anisopliae and V. lecanii. In case of B. bassiana isolate, the LC50 value was 6 × 104 conidia /ml while LT50 recorded 5.30 days at 5 × 107 conidia /ml. Results showed that
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2.6.4 Vacuum drying Amellal and Benamara (2008) investigated the drying ability of date (Phoenix dactylifera L.) pulp cubes from three Algerian common varieties (Mech - Degla, Degla - Beida, and Frezza). Drying process was carried out under partial vacuum (200 mbar) at 60, 80, and 100° C. Compared to the Newton model, the Henderson and Pabis model better described drying kinetic of Mech - Degla and Frezza pulps at 60 and 80° C with a mean relative error (MRE) not higher than 6.07%. The same model
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Addition Sequence B(ml) S(ml) T(ml) Working reagent Distilled water Uric acid Standard (S) Sample 1.0 1.0 1.0 0.02 -- -- -- 0.02 -- -- -- 0.02 Mix well and incubate at 37oC for 5 min. or at R.T. (25oC) for 15 min. Measure the absorbance of the standard (Abs.S), and Test Sample (Abs.T) against the Blank, within 30 min. Calculations Uric Acid in mg/dl = Abs.T X 8
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active site which produces water and oxygen. Therefore, as the amount of hydrogen peroxide increased then the displaced oxygen will increase but eventually it should level out due to the effect of substrate concentration on enzyme activity (Allot, 99-100, 2014). Catalase is an enzyme that is found in the liver of animals and it aids to catalyze the breakdown of hydrogen peroxide into water and oxygen (Allot, 99-100, 2014).
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ABSTRACT: Cells are composed of various molecules that vary in structure and function. It’s important to study the treatment that these molecules receive when they enter the body after being occupied from the outside sources in the form of different nutrients. The purpose of this lab is to test the process of hydrolysis occurring in different macromolecules by using the biochemical tests. This lab also indicates the hydrolysis of macromolecules when heat or acidity is applied to the molecules or
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A. Generate a fermentation profile for each of the organisms you tested. Glucose fermentation shows the ability of a bacterium to ferment carbohydrate as well as its ability to convert end products (pyruvic acid) into gaseous byproducts (Levinson, 2014). During this experiment 2 of the Staphylococcus epdidermidis tubes turned yellow. It was difficult to tell if there was an air bubble in any of the tubes that turned yellow, so I may be wrong on some of the fermentation profiles. The S. cerevisiae
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sources. They contribute to the body’s structure and help regulate body processes. Some minerals help regulate water balance; others help regulate energy metabolism, and some effect growth and development. “Many minerals act as cofactors needed for enzyme activity.” (WileyPlus) Minerals interact with other minerals as well as other nutrients and other components of the diet. Water is the one nutrient that is often overlooked. It accounts for 60% of our body water and is found inside cells and
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United States Patent [191 [11] 4,418,086 Marino et a1. [45] Nov. 29, 1983 [54] EXPANDED TEXTURED PROTEIN PRODUCT AND METHOD FOR MAKING SAME [56] References Cited U.S. PATENT DOCUMENTS 3,623,885 11/1971 3,745,021 7/1973 [75] Inventors: Richard P. Marino, Bedford Hills; Hamdy .......................... .. 426/802 X Middlesworth et a1. ..... .. 426/805 X 3,857,968 12/1974 3,968,269 7/1976 Haas et a1. .................... .. 426/805 X Payne et al. .. .
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