...The purpose of this experiment is to find the unknown concentration of two different proteins; the gamma globulin, and the bovine serum albumin. The measurement is done in the spectrophotometer by using the method of the Bradford Essay with the Coomassie Brilliant Blue G-250 dye, as a reagent. To identify the unknowns, first a colorimetric assay of both proteins with known concentrations is executed, and the data in plotted in two standard curves respectively. Finally, the unknown absorbance measurements are compared with the standard curves to find the absolute concentration, which is the most accurate measurement. Introduction Life as we know it is possible for the presence of the same elements repeatedly distributed throughout the matter that surrounds us in nature. These elements form molecules that in turn are the building blocks of every biological cell. Significantly, the information obtained from nature to understand life and evolution are the product of an applied scientific method, which has been improved by the invention and use of technology over time. Nowadays, Scientific are able to measure the components of specific samples precisely, but there are cases when limitations are present in their investigations. For example, that is the case of the measurement of proteins, which is not as easy as looking a sample through a microscope to be able to count how many proteins are present in the biological sample. Proteins are an...
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...London School of Engineering and Materials Science Laboratory report writing instructions DEN101 - Fluid Mechanics 1 Flow Rate Measurement Experiment A. Student Student Number: 1234567 Version 2.0, 27 November 2010 Template for Word 97-2003 Abstract This document explains what is expected in your Fluids 1 lab report. The sections that should be covered are outlined and a structure you could follow is proposed. Detailed advice on how to edit the report is given. The document concludes with the marking criteria for this lab report. Table of Contents Abstract 2 1. Introduction 3 1.1. Writing 3 1.2. Editing and formatting 3 1.3. Content of the introduction 4 2. Background and theory 4 3. Apparatus 4 4. Test 4 5. Experimental procedure 4 6. Results 5 7. Discussion 5 8. Conclusions 5 9. References 5 10. Appendix A: Marking criteria 6 Introduction Before starting to write a report, you should think about what is your audience. Am I writing for colleagues who want a lot of detail how it is done, or am I writing for my boss who just wants an executive summary as he has no time for details? In general, there is not a single type of audience and we have to make our writing suitable for the detailed read, as well as the fast perusal. To understand what is required from you in this report, please have a look at the marking criteria in the Appendix. 1 Writing To limit...
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...(Simple Diffusion) Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. The driving force for diffusion is You correctly answered: b. the kinetic energy of the molecules in motion. 2. In diffusion, molecules move You correctly answered: a. from high concentration to low concentration. 3. Which of the following dialysis membranes has the largest pore size? You correctly answered: d. 200 MWCO 4. Avogadro's number is a constant for the number of You correctly answered: b. molecules. 01/14/16 page 1 Experiment Results Predict Question: Predict Question 1: The molecular weight of urea is 60.07. Do you think urea will diffuse through the 20 MWCO membrane? Your answer : c. No, not at all. Predict Question 2: Recall that glucose is a monosaccharide, albumin is a protein with 607 amino acids, and the average molecular weight of a single amino acid is 135 g/mole. Which of the following will be able to diffuse through the 200 MWCO membrane? Your answer : b. both glucose and albumin Stop & Think Questions: The reason sodium chloride didn't diffuse left to right is that You correctly answered: c. the membrane pore size was too small. Glucose is a six-carbon sugar. Albumin is a protein with 607 amino acids. The average molecular weight of a single amino acid is 135 g/mole. There is no reason to run these solutes at the 20 MWCO because You did not answer this question. Correct answer: d. glucose and albumin are both too large...
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...Exp’t 112 Isolation of Casein, Lactose, and Albumin from Milk Adapted by R. Minard (Penn State Univ.) from Introduction to Organic Laboratory Techniques: A Microscale Approach, Pavia, Lampman, Kriz & Engel, Saunders, 1990. Revised 3/20/2000 PreLab Exercise: Draw a mechanism for the acid-catalyzed hydrolysis of the acetal bond in lactose; see reaction below. Hint: your textbook may be of some help; first identify the functional group that is undergoing a reaction and consider the catalyzed used. H HO OH CH2OH O O H OH H2C OH O HO HO OH OH H2C OH O HO HO + HO OH H2O OH CH2OH O HO H + D -Galactose D -Glucose OH α-Lactose: D -Galactose+ D -Glucose Introduction: The Chemistry of Milk Milk is a food of exceptional interest. Not only is milk an excellent food for the very young, but humans have also adapted milk, specifically cow’s milk, as a food substance for persons of all ages. Many specialized milk products like cheese, yogurt, butter, and ice cream are staples of our diet. Milk is probably the most nutritionally-complete food that can be found in nature. This property is important for milk, since it is the only food young mammals consume in the nutritionally significant weeks following birth. Whole milk contains vitamins (principally thiamine, riboflavin, pantothenic acid, and vitamins A, D, and K), minerals (calcium, potassium, sodium, phosphorus, and trace metals), proteins (which include all the essential amino acids), carbohydrates (chiefly lactose), and lipids...
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...The purpose of the enzyme lab conducted was to observe the chemical composition of cells. In order to do so we tested for the presence of organic molecules. Molecules are what forms when atoms bond together. Organic molecules of cells include proteins, carbohydrates, and lipids, which are composed of smaller molecules known as monomers and polymers. Polymers are joined monomers. A chemical reaction links monomers together occurs and releases a water molecule, this is called dehydration synthesis. Hydrolysis separates polymers into monomers by using water to break bonds. Organic catalysts called enzymes are proteins that increase the speed of a chemical reaction. In the lab we used Biuret reagent to test for proteins, iodine solution to test for starch, paper to test for lipids. In the first lab, we tested for the presence of proteins in samples by using blue solution called Biuret reagent, which changes to purple when a protein is present and pinkish-purple for peptides. First test tubes were marked at 1cm and then filled to the mark with water, albumin, pepsin, and starch. Next, five drops of Biuret reagent was added to the sample, covered with Parafilm, and swirled to mix. The water remained clear, indicating the sample lacked the presence of proteins, and thus was our negative control. The albumin sample observed changed to an orange-purple color, indicating the presence of protein. The peptin sample changed to a pink-purple hue, testing positive for presence of peptides...
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...Associate Degree Nursing Program NURS 122 Concept Care Map Evaluation Rubric: Maternal-Newborn |Value/categories |0 |1 |2 | |Assessment with Pathophysiology | < 90% data filled |> 90% data filled in |Complete with | | |incorrectly |correctly |Pathophysiology | |Lab work/rationale or reason for| | | | |labs | | | | | |0 |1 |2 | | |95% lab work and reasons | | |documented |documented |documented | |Step 3 - Care Map |0 |1 |2 | |Problem category Physiological |2 appropriate problems with|3 appropriate problems with|4 or more appropriate | |and psychological related to |Reason for admit and key |Reason for admit and key |problems with Reason for | |stages of postpartum |assessments |assessments |admit and assessments | | ...
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...stated that his father and paternal grandmother had developed diabetes. The other risk factor is obesity because B.Z. is 5’8” and 210lbs, which is very much over weight for his height. The pathophysiology of NIDDM, which is a non-insulin dependent diabetes mellitus and the body stops producing enough insulin for the body. The body does not have any ketones and occurs more frequently in people over the age of 30. The signs and symptoms of NIDDM are a delay in the secretion of insulin, it gets worse with age, Muscle and adipose tissue are most resistant, you will have high blood sugar levels, and the liver will initiate gluconeogenesis. The basic defect in this type of diabetes is genetics and obesity. 2. The significance of B.Z.’s report of Polydipsia and Polyuria are that the signs and symptoms are very similar to the signs and symptoms of diabetes. The 3 P’s in diabetes are polyuria, polydipsia and polyphagia. Polyuria is increased urine output. Polydipsia is increased thirst. Polyphagia is increased eating or hunger. The water is pulled intracellular causing dehydration. - The recent weight loss of B.Z. is due to the fluid in the osmolarity levels and the loss of body tissue. - Orthostatic Hypotention is an abnormal decrease in blood pressure when a person stands up. B.Z. has orthostatic hypotension because he is not getting enough blood flow to the brain. 3. Hyperosmotic hyperglycemic nonketotic coma is diabetes characterized by high blood levels of glucose...
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...tests should be carried out in a water bath at 95oC. Direct heating of test-tubes should not be taking place. Apparatus & Equipments: Test tubes Test tube rack Boling tubes Wooden holder Water bath, 95oC Spatula Materials: Iodine 0.1 M hydrochloric acid Sudan III Starch solution Corn oil Egg albumin 1% copper sulphate solution 1% sucrose solution 0.1 M Sodium hydroxide 1% glucose/fructose/lactose solution Absolute ethanol DCPIP (dichlorophenolindophenol) solution Ascorbic acid Introduction The nutrients in the food you eat supply your body with energy for growth and repair. These principle substances include carbohydrates, proteins, fats, minerals and vitamins. We can test for the presence of these important compounds in food by using chemical reagents that react in predictable ways in the presence of these nutrients. Please refer to the notes given above on: How to record qualitative data. (Marks will be awarded based on proper recording.) What to do if you don’t obtain the desired results. Flowchart Students will be allowed to proceed with the experiment only if they have come into the laboratory with a flowchart of the day’s experiment. Lab manual version 6_201505 FHSB1214 Biology I & FHSC1214 Fundamentals of Cell Biology 14 Procedures: Part 1: Identification of Carbohydrates (A) Test for reducing sugars The reducing sugars include all monosaccharide, such as glucose and fructose, and some disaccharides, such as maltose and lactose...
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...LabCorp SPECIMEN INFORMATION PATIENT INFORMATION REPORT STATUS: Caudill, Kyle ORDERING PHYSICIAN SPECIMEN: 19030556730 REQUISITION: 21990796 LAB REF NO: DOB: November 19, 1980 AGE: 34 GENDER: Male FASTING: Yes COLLECTED: 2015/07/09 14:21 Clinical Info: RECEIVED: 2015/07/09 17:09 FINAL NO CHANGES (DEMOGRAPHIC OTHER) ARE OR Stewart, Robert CLIENT INFORMATION 2015-07-12 06:39:00 -0700 Private MD Labs 445 Hwy 46S Suite 29-214 Dickson, TN 37055 REPORTED: 2015/07/12 06:39 Test Name CBC With Differential/Platelet WBC RBC Hemoglobin Hematocrit MCV MCH MCHC RDW Platelets Neutrophils Lymphs Monocytes Eos Basos Neutrophils (Absolute) Lymphs (Absolute) Monocytes(Absolute) Eos (Absolute) Baso (Absolute) Immature Granulocytes Immature Grans (Abs) Comp. Metabolic Panel (14) Glucose, Serum BUN Creatinine, Serum eGFR If NonAfricn Am eGFR If Africn Am BUN/Creatinine Ratio Sodium, Serum Potassium, Serum Chloride, Serum Carbon Dioxide, Total Calcium, Serum Protein, Total, Serum Albumin, Serum Globulin, Total A/G Ratio Bilirubin, Total Alkaline Phosphatase, S AST (SGOT) ALT (SGPT) Testosterone, Serum Testosterone, Serum Comment: Adult male reference interval up to 40 years old. Luteinizing Hormone(LH), S LH FSH, Serum FSH Result Flag Reference Range Lab 11.6 5.45 16.3 48.1 88 29.9 33.9 14.2 320 60 31 4 4 1 6.9 3.6 0.5 0.4 0.1 0 0.0 HIGH 3.4-10.8 x10E3/uL ...
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...Hepatic Encephalopathy Name________ Directions: Gender: Male Age: 47 Setting: Hospital Ethnicity: African American Preexisting Conditions: Cirrhosis secondary to alcohol hepatitis, hypertension, esophageal varices Coexisting Conditions: Disability: Unemployed (on disability) for past four years Socioeconomic: Married, father of two boys (ages 19 and 17 years old), history of drinking one quart of hard liquor each day for three years prior to diagnosis of cirrhosis Pharmacologic: Lactulose (Cephulac), neomycin sulfate (mycifradin sulfate) Client Profile: Mr. Escobar is a 47-year old male with a history of cirrhosis. He lives with his wife and teenage sons. His wife brought him to the emergency department today because she noticed that her husband had increasing confusion and lethargy and was having difficulty walking. His wife states, “ He is probably acting a little fun because he is sleep deprived. He hasn’t slept very much in the past few days.” Case Study: Mr. Escobar is afebrile. His blood pressure is 136/68, pulse 88, and respiratory rate 18. His oxygen saturation is 98% on room air. He is awake, alert, and oriented to person only. His speech is slow and he appears tired. The nurse notices a foul odor to his breath. Upon physical examination, he is found to have a slightly distended abdomen. The health care provider (HCP) does not note any asterixis. The HCP requests an abdominal ultrasound, which reveals fatty infiltration of the...
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...Chief Complaint: Mr. Cane is a 64-year-old male who has presented in clinic for recent tremors that are more prevalent at rest. History of Presenting Illness: Mr. Cane describes that over the past three months he has had “shaking” that he cannot control, particularly when he rests. He has noticed that these tremors have been affecting his right arm in particular. His everyday activities are being impacted by these symptoms and he is concerned that he may not be adequately perform his job anymore. He also reports that his limbs feel stiff. His wife reports that she has noticed slowness in his overall movements and that he has been depressed. His speech has also been noted to have become softer recently. Past Medical History: Mr. Cane reports...
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...Content Frame Skip Breadcrumb Navigation Home arrow Chapter 2 arrow Nursing Care Plan Nursing Care Plan Nursing Diagnosis Impaired Verbal Communication r/t sedation, presence of artificial airway, or decreased level of consciousness Long Term Goal: Patient is able to use a form of communication to get needs met and relate to his environment Short Term Goals / Outcomes: Patient and nurse will establish a means of communication Patient will be able to effectively communicate and needs Intervention Rationale Evaluation Assess the patient’s primary and preferred means of communication (verbal, written, gestures) Communication can be frustrating for both the nurse and patient. It is critical that the nurse and patient determine the best method for each patient. Patient can write words clearly on paper Assess the patient’s preferred language and ability to understand written words, pictures and gestures The nurse can not assume that the patient is grasping the information that is provided. In recognition of the vast array of cultures and physical challenges that patient’s face, it is the nurse’s responsibility to communicate effectively Patient speaks and reads English. Recognize that the presence of an artificial airway will hinder the patient’s ability to communicate When air does not pass over the vocal cords, sounds are not produced. Other methods of communication will have...
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...Care Plan : Pregnancy Induced Hypertension (PIH) Patient Conference Report History of events leading to admission: This is a 46 y/o female that was admitted to Brandon Hospital. She is 28 weeks gestation with twins. Medical diagnosis: Pregnancy Induced Hypertension Past Medical History: Seizure disorder for which she takes Lamictal, infertility, 2nd. Invitro with twins, she has a sinus infection. Past Surgical History: Laparoscopy for endometriosis X3, surgery for broken jaw, tonsillectomy, Pertinent Lab Results: Most recent labs done 2/22/15, (CBC) NA+ 156, BUN- 28, Albumin- 8.0, Protein- 10.0, Creatinine- 1.3, Pertinent diagnostic results: Chest X-ray: pulmonary edema and cardiomegaly, cardiac catheterization, EKG: atrial fibrillation. Lists of Medications: Amoxicillin, Docosate sodium, Labetalol HCL, Lamotrigine (lamictal). Allergies: Macrobid Code status: Full code Vital Signs: T 99.2F, oral P 80, regular B/P 186/100 SaO2 100% Weight- 160lbs. Ht. 5’5 List 3 pertinent medications given by you on your shift. List actions and indications, side effects, and nursing considerations: |Name: |Amoxicillin | |Action and indications. |Binds to bacterial cell wall, causing cell death, spectrum of amoxicillin is broader than penicillin. Treatment...
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...Associate Level Material Medical Report This assignment is for you to create a screening tool for potential hires in your health care facility. As the health care administrator, you would want to ensure that your future employees have a strong understanding of medical reports and medical terminology. You are writing these reports for the applicants to read, interpret, and answer a set of questions you have developed. Refer to the samples of medical records reports on pages (142-144, 196, & 261-263) of the textbook. Each medical record should be completed and contain two questions you would ask of the potential hires. The following suggestions will help you get started: • Sometimes it is easier to start at the end. Think of the diagnosis the patient will receive. If you know what the end diagnosis will be, it makes it easy to know what symptoms, signs, and diagnostic methods would be used to achieve that diagnosis. • For the History of Present Illness, consider what questions the physician might ask the patient about his or her chief complaint and symptoms and then chart that in this section. This section serves as an account of what the patient would report, based on their symptoms. Remember, symptoms are subjective, in that they are conditions experienced by the patient, and are therefore included in the patient history. • For Past Medical History, document anything the patient may indicate in terms of past medical conditions that would be relevant...
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...Critical Care Case Study Crystal Meyer Mohave Community College Nursing 222 Mrs. Michelle Christensen April 1, 2014 Critical Care Case Study ADMISSION TC is a 61-year-old English speaking Caucasian female born on April 29, 1952. She weighs 99.7 Kg and is 5 feet, 5 inches in height with a BMI of 35.84. On March 5, 2014, TC was brought into the emergency department after her daughter-in-law called 911 when she found TC unresponsive at home in her bathroom. When paramedics arrived, she was found to be cool, pale, and diaphoretic with oxygen saturations in the high 70’s. Emergency responders placed a non-rebreather high flow oxygen mask and her oxygenation began to improve with saturations in the low 90’s. Upon arrival to the emergency department, TC’s vital signs were as follows: T 97.4; P 97; BP 120/95 mm Hg; RR 15 per minute; and O2 sats of 98% via NRB oxygen mask on 8L. A chest x-ray (CXR) revealed no abnormality and lungs were determined to be grossly clear. However, TC was checked for a pulmonary embolism via a pulmonary artery angiogram with IV contrast and found to have a large clot burden with a small saddle embolism. TC also complained of right ankle pain. An X-ray of her right ankle revealed a distal tib/fib fracture, which was presumed to be related to her fall during her hypoxic episode. With these findings, TC was admitted to the Intensive Care Unit of Kingman Regional Medical Center and placed on an NPO diet in preparation for placement of an inferior vena...
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