Introduction Bacteria are microscopic unicellular prokaryotic organisms characterized by the lack of a membrane-bound nucleus and membrane-bound organelles. They are remarkably adaptable to diverse environmental conditions and are found in bodies of all living organisms and on all parts of the earth. The purpose of microbial biochemical tests is to identify the unique traits it yields and with that knowledge we can then categorize them in groups and specify them by scientific name. These experiments
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Unit 2 Assignment, Yvonne McAlpin For this week’s assignment covering disease research, I chose pancreatitis. I chose this because my friend was just recently diagnosed with this. She is a very healthy person, in fact healthier than I and a little younger. I thought it would be a perfect research topic. Pancreatitis means inflammation of the pancreas that can appear in two very different ways. Acute pancreatitis is sudden while chronic pancreatitis is recurring or persistent. Some cases
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Enzymes: Virtual Lab Introduction: This is a virtual lab simulation of enzymes and substrates. It teaches about enzymatic activity and how it is affected by substrate concentration and pH. Students are to visualize the virtual lab as an actual lab and conduct the experiment as such. Purpose This investigation will determine the effects of substrate concentration and pH on the initial rate of an enzyme-catalyzed reaction. Materials Computer Pencil/pen Enzymes at various pH Substrates
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93 7plummer, D T, Elliot, B A, Cooke, K B and Wilkinson, J H (1963) Biochem J 87,416-422 8Zubay, G (1993) Biochemistry, Third Edition, p 663, Brown', Dubuque, Iowa '~Rawn, J D (1983) Biochemistry, p 675. Harper and Row, New York "~Horton, H R, Moran, L A, Ochs, R S, Rawn, J D and Scrimgeour, K G (1993) Principles of Biochemistry. pp 12.20-21, Neil Patterson Publishers/Prentice Hall, Englewood Cliffs, New Jersey I I Werner, R (1992) Essential Biochemistry and Molecular Biology, Second Edition, pp
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What is the effect of temperature, pH, and substrate concentration on Enzyme activity? Purpose: Enzymes are proteins that speed up chemical reactions in cells. They break down molecules called substrates. Each enzymes have only one substrate that breaks down. Enzymes are produced in the cells of the body and affect the rate of almost all the chemical reactions which take place in living organisms. The rate of enzymes activity is influenced by temperature, pH, and substrate concentration
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Shigella dysenteriae (far left). Pseudomonas aeruginosa (center) is a nonfermenter. The end product of glycolysis is pyruvate. Organisms that are capable of converting pyruvate to formic acid and formic acid to H2 (g) and CO2 (g), via the action of the enzyme formic hydrogen lyase, emit gas. This gas is trapped in the Durham tube and appears as a bubble at the top of the tube. Escherichia coli and Proteus
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Lindsey M. Maurice-Walker Week 6/7 Results Biochemistry Lab March 14, 2013 Results: Table 1 and Table 2, given below, display the average absorbance of the samples, the amount of protein in each sample, the activity value, the specific activity, and the molar absorptivity calculated from the above equations for Phosphatase and Invertase. Table 1. Value Summary for Phosphatase | | Abs400 | mg of protein | Units (activity)(mg/min) | Specific Activity(ug/min/mg) | CYE | 0.296333333 |
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The cytoplasm and extracellular environment of the cell are aqueous solutions. They are composed of water, which is the solvent, or dissolving agent, and numerous organic and inorganic molecules, which are the solutes, or dissolved substances. Organelle membranes and the plasma membrane are selectively permeable, allowing water to freely pass through but regulating the movement of solutes. The cell actively moves some dissolved substances across membranes, expending adenosine triphosphate (ATP)
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Enzymes are biological catalysts that carry out thousands of chemical reactions that occur in living cells. Most of them are proteins. They speed up metabolic reactions in the body but remain chemically unchanged themselves In the experiment, the enzyme amylase was involved.this enzyme takes part in the digestion ofcarbohydrate. digestion is the break down of large complex and insoluble food into small simple soluble molecules for the body to absorb it. starch is a complex, the body have to
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Introduction (5 marks) Enzymes are globular shaped proteins that are found throughout the body, with their main function being to act as biological catalysts. An enzyme can act to speed up or regulate the rate of the reaction, in order to maintain an efficient rate of biological reactions. Enzymes, whilst having an important role in the reaction of many chemicals within the body, are not consumed in the reaction, and so are able to catalyze many reactions in their life cycle. Enzymes are able to reduce
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