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Caenorhabditis Elegans Lab Report

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Introduction:
The fundamental objective behind the performance of this procedure is to surmise the mechanism of the transfer of genetic material and to accomplish this the model organism Caenorhabditis elegans were implored. The determination of the inheritance pattern of a particular unknown mutant gene in Caenorhabditis elegans was explored. Caenorhabditis elegans was chosen due to the relative ease of maintenance of the organism in laboratory setting, its small size and their short life cycle around 3 days as well as there small genome and anatomic simplicity2.
Methods:
A controlled mating environment must first be established to effectively determine the inheritance pattern of the mutant gene of interest. Caenorhabditis elegans have two distinct genders that are distinguishable by physical appearance under microscopic examination, male and hermaphrodite. …show more content…
Being that the distribution between sexes should be approximately 50/50.

Discussion: The parental generation of the three crosses contained true breeding hermaphrodites possessing a short and fat phenotype characteristic of dumpy #6 mutants and true breeding males displaying the wild type phenotype. Cross 1 and cross 3 suggest that mainly selfing amongst hermaphrodites had taken place. Statistical test of the probability that results of that nature would show that something other than mating between hermaphrodite and male had taken place. By reason to insufficient correlation between the results of each cross a definitive conclusion not be

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