...DNA Fingerprinting Using Agarose Gel S. Aaron Sowards Bio 122 Lab 04 Brianna Adanitsch Jakob Lester Minhenga Ngijoi 2/21/18 Dr. Chad R. Sethman Abstract DNA fingerprinting is the process of analyzing an individual’s DNA base-pair patterns. The DNA fingerprinting lab involved identifying the suspect using Agarose Gel and Polymerase Chain Reaction. It was found that suspect two s DNA matched the crime scene DNA. This is known because suspect twos DNA traveled the same distance as the crime scene DNA. DNA Fingerprinting Using Agarose Gel Introduction In 1984 Dr. Alex Jeffreys came up with deoxyribonucleic acid (DNA) fingerprinting, which is also known as DNA profiling or DNA typing. DNA fingerprinting is the analyzing...
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...In 2004, Mongelli, Reynolds and Steele studied the collision of the new technology of DNA fingerprinting which focuses in concluding the augmentation of DNA databases to include every convicted person, not just only the blood-and-guts crimes. This investigation can lead to its good effects on society, for putting one’s finger on unknown dead body, determining paternity, placing the suspect at the crime scene, more advanced leads, and connect crimes to identify the suspect. Meanwhile, their project aim is help to defeat and remove the public's doubt when it comes to DNA fingerprinting and help them know the information about the science in standing up for the use of DNA fingerprinting in the room in which legal cases are heard. Their study...
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...How has our knowledge of DNA improved the study of criminal forensics? Introduction Through genetics, the study of DNA, we are able to figure out what and how genes are responsible for many things like our hair color or why do some people look a lot like their parents and others don’t. It also allows us to understand better how species evolve and how are they related to each other. It is important to understand how DNA mutates, changes and replicates in order to get information about what mechanisms cause DNA to change. In the 1970s scientists developed a DNA sequencing technique and other methods to manipulate and analyze DNA. This gave them the basic tools to start exploring the DNA blueprint which provided the techniques for a vast international project called The Human Genome Project (MRC). The Human Genome Project which was a major international project with the goal of decoding all our genetic information by 2003. A rough draft was done in June 2003 and it was a huge milestone that helped us understand how our genes can determine who we are (Genome Project). Many of today’s advances in DNA and biotechnology allow scientists and medical doctors to potentially cure genetic disorders through gene therapy by inserting, deleting or manipulating genes (Tillery, page 686). Another use of DNA technology is the creation of mutation by transferring DNA from one organism to another through techniques like cloning and introducing new DNA sequence into an organism to alter...
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...How has the development of fingerprinting techniques in forensics increased the succession rate of convicting criminals from the period of 1890-1950? Introduction The birth of forensic science within the use of the police force revolutionised how crime investigations were conducted, via the work of the forensic scientists, or also known as ‘forensic officers’. This work would consist of collecting evidence from a crime scene such as looking for fingerprints on either a suspected murder weapon used by the murder or on a touchable surface which is in the crime scene where the suspect laid their hand on. Furthermore this collectivisation of evidence from the crime scene could mean collecting; foreign fibres (unusual fibres that seem to look like that they do not belong their i.e. due to their different colour, type of fabric or thickness of the fibre.); retrieving bullets and firearms form the crime scene and any possible DNA samples that can be collected in the crime scene such as blood spatter on the walls of a room, where in this case the crime was committed. This evidence would then be sent to be analysed by more forensic scientists in different subdivisions to help led a path to a final suspect, thus leading the police to a quick and successful conviction of a criminal and bring justice to their crimes. Overall this revolution of the police force saw the birth of a new era of fighting crime throughout the whole of the country, as well as throughout the rest of the...
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...‘unofficially’ regulate DNA analysis and force ‘private companies to adopt its technological system’” (Edmond 130). These debates in the courtroom have led to regulations that are in place to ensure the proper gathering and testing of DNA evidence so that only accurate evidence is admitted in court. Suspects have a right to a fair trial and following the DNA wars, fair trials include correct evidence to be presented otherwise they may be acquitted or a mistrial may be declared. One of the factors to ensuring correct evidence is a process known as the chain of custody. The chain of custody, if followed properly, keeps track of continuity of possession. Evidence must be correctly...
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...Jeffreys is a British geneticist who developed techniques for DNA fingerprinting and DNA profiling that are now used worldwide by investigators to bring justice to perpetrators as well as to resolve paternity and immigration disputes. After finishing his doctorate, he moved to the University of Amsterdam, where he worked on mammalian genes as a researcher, and then to the University of Leicester in 1977, where in 1984 he discovered a method of showing variations between people's DNA, formulating and developing genetic fingerprinting. Jeffreys says he had an “eureka moment” in his lab in Leicester after looking at the x-ray film image of a DNA experiment on 10 September 1984, which surprisingly showed similarities and differences...
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...geneticist who developed techniques for DNA fingerprinting and DNA profiling that are now used worldwide by investigators to bring justice to perpetrators as well as to resolve paternity and immigration disputes. Jeffreys was born into a middle-class family in Oxford, where he spent the first six years of his life until 1956, when the family moved to Luton, Bedfordshire. He attributes his curiosity and inventiveness to having been gained from his father, as well as his paternal grandfather, who held ma ny patents. When he was eight, his father gave him a chemistry set, which he enhanced over the next few years with extra chemicals, even including a small bottle of Sulphuric acid. He says he liked making small explosions, but an accidental splash of the sulphuric acid caused a burn, which left a permanent scar on his chin (now under his beard). His father also bought him a...
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...Research on DNA Forensics 1. Is DNA evidence perfect? 2. Should every prisoner have the right to reopen his or her case for DNA testing? Follow-Up Questions • Who should decide who does and does not have the right to reopen a case? • Who should pay for the testing? • How is the credibility of our legal system affected by the fact that numerous people have been convicted and then exonerated of a crime? 3. Would you vote to convict a person of a crime based solely on DNA evidence? Follow-Up Questions • Would you be more or less comfortable with convicting a person of a crime based solely on DNA evidence than with convicting a person based on eyewitness testimony? • Other than scientific evidence, what else might influence the decision of a jury? • If the case were heard by a technology-savvy jury, would the verdict have been different? • If the memories of an eyewitness are open to contamination, how can we trust what honest people see? • Should eyewitnesses that wrongly implicate a person for a crime be held accountable for damages? • Should a wrongly convicted person seek damages and restitution? What would be the implications for our legal system? • How does the questionable nature of eyewitness testimony affect the use of DNA forensics? Internet Research links Killer’s Trial (http://www.pbs.org/wgbh/nova/sheppard/) From WGBH Boston, this is a collection of on-line resources that accompany a NOVA special investigating the Sam Sheppard murder...
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...Unit 6 Lab 1: DNA Fingerprinting Arielle Chambers Ms. A. Wain ITT Technical Institute SC 2730 9 February 2015 1. What kinds of patient samples are used for the purpose of identifying possible pathogens? a. Fluid from lymph nodes b. Stool sample c. Urine sample d. Blood sample e. Sputum sample 2. What does PCR do, how does it work, and why is it useful? PCR is also known as the Polymerase Chain Reaction, it is a fast and inexpensive way to amplify small segments of DNA (genome.gov). PCR works by amplifying a segment of DNA, the sample is heated first the DNA denatures, or separates into two pieces of single-stranded DNA (genome.gov). After that an enzyme called Taq polymerase synthesizes builds two new strands of DNA, it uses the original strands as templates (genome.gov). The process results in the duplication of the original DNA, each of the new molecules contain one old and one new strand of DNA (genome.gov). Each of these strands can be used to create two new copies, and so on, and so on. The cycle of denaturing and synthesizing new DNA is repeated as many as 30 or 40 times, leading to more than one billion exact copies of the original DNA segment (genome.gov).The entire cycling process of PCR is automated and can be completed in just a few hours. It is directed by a machine called a thermo cycler; it is programmed to alter the temperature of the reaction every few minutes to allow DNA denaturing and synthesis (genome.gov). PCR is useful...
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...1)Introduction; Crime today is at an extreme high. However, forensic science has been there to help solve every crime committed Forensic science is the scientific method of gathering and examining information about the past. The word forensic comes from the Latin forēnsis, meaning "of or before the forum. The word forensic basically means the key to solve a crime.This is the technology used to help forensic teams to analyze and solve crimes.- This is especially important in law enforcement where forensics is done in relation to criminal or civil law,[1] but forensics are also carried out in other fields, such as astronomy, archaeology, biology and geology to investigate ancient times. Forensic Science is used to Identify Criminals Rape, murder, theft, and other crimes almost always leave a devastating mark on the victim. . In modern forensic science, the crime laboratories include photography section, Evidence storage section, identification section, chemistry section, General examination section, Fire arms section, instrument section and crime scene search section. 2)Origins of forensic science: In 16th-century Europe, medical practitioners in army and university settings began to gather information on the cause and manner of death. Ambroise Paré, a French army surgeon, systematically studied the effects of violent death on internal organs.[9][10] Two Italian surgeons, Fortunato Fidelis and Paolo Zacchia, laid the foundation...
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...and trust on forensic evidence is unshakable. In particular, jurors significantly rely on DNA analysis, scientific evidence, and testimonies by forensic experts to help decide the outcome of most criminal cases. Hence, justice and freedom for an individual can depend on the proficiency, reliability and efficiency of a forensic laboratory and their forensic analysts. Investigative or methodological forensic errors either on...
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...DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a person's DNA makeup, which can also be used as the person's identifier. DNA profiling should not be confused with full genome sequencing.[1] It is used in, for example, parental testing and criminal investigation. Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different to distinguish one individual from another, unless they are monozygotic twins.[2] DNA profiling uses repetitive ("repeat") sequences that are highly variable,[2] called variable number tandem repeats (VNTR), particularly short tandem repeats (STR)s. VNTRs loci are very similar between closely related humans, but so variable that unrelated individuals are extremely unlikely to have the same VNTRs. The DNA profiling technique was first reported in 1984[3] by Sir Alec Jeffreys at the University of Leicester in England,[4] and is now the basis of several national DNA databases. Dr. Jeffreys's genetic fingerprinting was made commercially available in 1987, when a chemical company, Imperial Chemical Industries (ICI), started a blood-testing centre in England.[5] Contents [hide] 1 DNA profiling process 1.1 RFLP analysis 1.2 PCR analysis 1.3 STR analysis 1.4 AmpFLP ...
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...DNA is a molecule that contains the body’s genetic information. ”DNA typing is also known as DNA profiling and genetic fingerprinting. Forensic investigators use this method to identify the suspects involved in a criminal case(s). “Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different to distinguish one individual from another.” (Accessexcellence.org) A Mr. Alec Jefferys first documented the DNA typing procedure in 1984 at the University of Leicester in England. Inside ones DNA, we find what is called Short Tandem Repeats (STR). It is known why STR exist, but what is significant is that over 30 percent of human DNA is made up by these STR. “STRs are locations on the chromosome that contain short sequence elements that repeat themselves with in the DNA molecule.”(Saferstein, R pg.397) With Polymerase Chain Reaction or PCR technology, DNA research has made leaps and bounds. This method greatly enhances a single piece of DNA and makes thousands of copies of that particular DNA sequence. Another reason PCR is proven beneficial is that it moves shorter strands of DNA that are supposed to be more stable and less subjected to degradation, as oppose to the longer strand that break in bad environment atmospheres. Particularly PCR can enhance the STRs found in individuals blood and can be used to tell the difference between different individuals DNA, thereby proving a person innocent or guilty. In criminal investigations today DNA is used...
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...Deoxyribonucleic acid, commonly known as DNA, is the carrier of genetic information and the building blocks of all organisms. It is also the building block for both the defense and prosecution in criminal cases. This paper will explore the history of DNA profiling in the criminal justice system. While it is a common misconception that James Watson and Francis Crick discovered DNA in the 1950s, it was actually discovered in the 1800s. The molecule now known as DNA was first identified in the 1860s by a Swiss chemist called Johann Friedrich Miescher. Johann set out to research the key components of white blood cells, part of our body’s immune system. The main source of these cells was pus-coated bandages collected from a nearby medical clinic....
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...General Biology 1005 Laboratory Schedule BCC Central Campus Fall Session 2 2011 All required readings should be completed before coming to the lab. Lab coats, closed toe shoes and eye protection are required for all laboratories. |Dates |Days |Lab # |Lab Title |Required Pre Lab Reading | | | | | |Hoefnagels | | | | | |(1st Edition) | |8/22-23 |M-T |1 |Introduction: Safety and Scientific Method |Pages 11-16 | |8/25-25 |W-Th |2 |Forensic Microscopy |Pages 50-53 | |8/29-30 |M-T |3 |Cell Chemistry I: Amino Acids, Water and pH |Pages 29-34, 37-40 | |8/31-9/1 |W-Th |4 |Cell Chemistry II: Carbohydrates and Lipids |Pages 33-37 | |9/5-6 |M-T | |LABS CLOSED | | | | | |LABOR DAY ...
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