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Eukaryotes Cell Cycle

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In eukaryotes, reproduction at a cellular level requires interphase, mitosis, and cytokinesis- collectively known as the cell cycle. Interphase includes the three stages: G1, S, and G2. The G1 (growth) phase is where the cell prepares for DNA and chromosome replication, taking approximately 10 hours. The S phase, 9 hours long, is where the DNA and chromosomes actually replicate giving 2 exact copies called sister chromatids. In G2, the cell takes four hours to prepare for cell division. Mitosis itself takes only an hour and occurs in both haploid and diploid cells. The duplicated chromosomes separated into two nuclei. The five stages of mitosis are prophase, prometaphase, metaphase, anaphase and telophase. After mitosis is complete, cytokinesis …show more content…
Centrioles form on opposite pole ends of the cell and the mitotic spindle appears outside of the nucleus while the nucleolus shrinks. Prometaphase occurs before the nuclear envelope breaks down. In prometaphase, the spindle fibers attach to the kinetochore (multiprotein complex) which bind to each centromere. During metaphase, the kinetochore microtubules orient chromosomes so the centromeres become aligned at the metaphase plate. Anaphase is when the paired chromosomes separate at kinetochores. The sister chromatids get separated and are pulled to opposite sides. In telophase, the two sets of daughter chromosomes reach their opposite sides. The nuclear envelope reforms while the chromosomes become less condensed, and the spindle fibers …show more content…
The two new nuclei are compartmented into separate daughter cells. The partitioning is different in both the plant and animal cells. In plant cells, a cell plate is synthesized between the two daughter cells. In animal cells, cytokinesis results in the cell membrane being pinched inward as a cleavage furrow leaving two daughter cells, each with one nucleus.
Meiosis follows phases similar to mitosis, but there are two successive division of a diploid nucleus after one DNA replication. At the end of meiosis, the chromosome number of the parent cells are reduced so that the four daughter cells have one half the number of the parent cell. meiosis II, follows the same steps as mitosis, in which the sister chromatids are separated. At the end of meiosis II, four haploid daughter cells are produced as opposed to the two diploid daughter cells that are produced after mitosis.
We examined the slides (mitochondria of turtle liver cells, nucleic acids of onion root tip, generalized animal cell, human male chromosomes, and human female chromosomes) using a compound light microscope with different magnifications to identify all stages. The objective of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals so we then further assessed them in the onion root tip cells using the compound light

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