...Coordinator : Dr Chee Swee Yong Lecturers : Ms Chang Chew Cheen, Dr Chee Swee Yong, Dr Lim Tuck Meng, Dr Sim Yoke Leng |Lab group |Lab A |Lab B | |Venue |D012A |D012B | |Monday @ 9.00 am – 11.30 am |P5 |P1 | |Monday @ 3.00 pm – 5.30 pm |P2 |P3 | |Wednesday @ 9.00 am – 11.30 am |P3 |P4 | |Wednesday @ 3.00 pm – 5.30 pm |P1 |P2 | |Thursday @ 3.00 pm – 5.30 pm |P4 |P5 | |Week |Experiment |Lab group |Lecturer | |1 |Briefing by HoD |Lab A/ Lab B |Dr Sim KM | | |Expt 1: Investigating the properties of Period 3 oxides |Lab A |Dr Chee SY | | | | | ...
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...Enzyme Kinetics: Inversion of Sucrose Abstract Enzymes are a class of proteins that catalyze practically all biochemical reactions. The enzymatic reactions were looked at by the use of two comparable reactions of enzyme invertase and a acidic form of the reaction. The order of the nonacidic enzymatic reaction was zero order due to substrate concentration and due to the fact that the reaction was time dependent. It is also due to having large R2 value at 0.9932 for D run and a smaller R2 value at 0.9902 for C run. The acidic runs had a first order reaction, which had a lower R2 values at 0.9028 for run D and 0.9028 for run C. Also, the percent error in run C at 33.31% was found to be much lower than the percent error in run D at 55.77%, which mean the concentration of run C is more effective than run D. I. Introduction In this experiment the chemical kinetics of the enzyme catalyzed inversion of sucrose was studied. The reaction that we will study in this experiment is the inversion of sucrose, catalyzed by the enzyme invertase that is derived from yeast: The rate of reaction of this reaction was compared to the same reaction that is to be catalyzed by hydrogen ions. Enzymes make up an important class of proteins that are used to catalyze a wide array of biochemical reactions. The enzyme that was used to catalyze the reactions in this lab experiment was the enzyme...
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...Determination of the Reaction Rate of Mg and HCl Alex Skartvedt Alex Skartvedt, Melanie Ho, Christina Olson, Thor Swerdykiak November 11, 2015 Fall 2015 Semester Abstract: The experimental rate law of the reaction: 2HCl(aq)+Mg(s)→MgCl2(aq)+H2(g) is being determined because kinetics play a large role in the reactions happening in everyday life. The rate law, k value will be obtained in the experiment. To obtain the information a pressure probe was connected to an Erlenmeyer flask in order to record the rate of the reaction of magnesium ribbon and hydrochloric acid. The order of HCl and Mg were then determined using the information found in from the reaction. The order in HCl and Mg were found to be less than the expected...
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... A pipette (also called a pipet, pipettor or chemical dropper) is a laboratory instrument used to transport a measured volume of liquid. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Lab dish washing Cleaning laboratory glassware isn't as simple as washing the dishes. Here's how to wash your glassware so that you won't ruin your chemical solution or laboratory experiment. You can rinse the glassware with the proper solvent, then finish up with a couple of rinses with distilled water, followed by final rinses with deionized water Water Soluble Solutions (e.g., sodium chloride or sucrose solutions) Rinse 3-4 times with deionized water then put the glassware away. Water Insoluble Solutions (e.g., solutions in hexane or chloroform) Rinse 2-3 times with ethanol or acetone, rinse 3-4 times with deionized water, then put the glassware away. In some situations other solvents need to be used for the initial rinse. Lab chemical stock maintenance • Remove items from the written inventory as they are disposed or used • Record all new orders • Record the received date on...
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...The premise of this lab is to measure our enzymatic activity of Alkaline phosphatase, we began measuring our activity by applying different substrate concentration of p-nitrophenyl phosphate in relationship to absorbance over the span of 70 seconds, the data obtained in table 5.1, the different substrate concentration 0.9mM, 0.675mM, 0.45mM, 0.225mM, 0.09mM, 0.045mM, 0.0225mM. keeping our enzyme constant at 100μL, what we concluded is a significant decrease on the absorbance as we decrease the substrate concentration, for example our initial reading of absorbance at 0.09mM concentration was 0.558, the same reading at 0.0225mM was significantly lower (0.176) thus indicating that substrate concentration does influence absorbance if the enzyme concentration remains constant....
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...8. Assessment System and Breakdown of Marks: Continuous assessment: 50% - Theoretical Assessment (Tests/Quizzes/Case Studies) (30%) - Practical Assessment (Lab reports/Lab tests) (20%) Final Examination 9. 10. 50% Academic Staff Teaching Unit: Objective of Unit: The aims of this course are to enable students to: • appreciate the important role of physics in biology. • elucidate the basic principles in introductory physics enveloping mechanics, motion, properties of matter and heat. • resolve and interpret quantitative and qualitative problems in an analytical manner. • acquire an overall perspective of the inter-relationship between the various topics covered and their applications to the real world. • acquire laboratory skills including the proper handling and use of laboratory apparatus and materials. 11. Learning Outcome of Unit: At the end of the course, students will be able to: 1. Identify and practice the use of units and dimensional analysis, uncertainty significant figures and vectors analysis. 2. Apply and solve problems related to translational and rotational kinematics and dynamics in one and two dimensions. 3. Apply and solve problems related to the conservation of energy. 4. Identify and compare the state and properties of matter, and fluid mechanics with various related principles, and kinetic theory of gases. 5. Identify the difference in temperature scales and solve problems related to the concept of heat and heat...
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...8. Assessment System and Breakdown of Marks: Continuous assessment: 50% - Theoretical Assessment (Tests/Quizzes/Case Studies) (30%) - Practical Assessment (Lab reports/Lab tests) (20%) Final Examination 9. 10. 50% Academic Staff Teaching Unit: Objective of Unit: The aims of this course are to enable students to: • appreciate the important role of physics in biology. • elucidate the basic principles in introductory physics enveloping mechanics, motion, properties of matter and heat. • resolve and interpret quantitative and qualitative problems in an analytical manner. • acquire an overall perspective of the inter-relationship between the various topics covered and their applications to the real world. • acquire laboratory skills including the proper handling and use of laboratory apparatus and materials. 11. Learning Outcome of Unit: At the end of the course, students will be able to: 1. Identify and practice the use of units and dimensional analysis, uncertainty significant figures and vectors analysis. 2. Apply and solve problems related to translational and rotational kinematics and dynamics in one and two dimensions. 3. Apply and solve problems related to the conservation of energy. 4. Identify and compare the state and properties of matter, and fluid mechanics with various related principles, and kinetic theory of gases. 5. Identify the difference in temperature scales and solve problems related to the concept of heat and heat...
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...of Central Florida CHM 2046L Chemistry Fundamentals Laboratory (1 Credit) Course Syllabus Graduate Laboratory Instructor: Office: Phone: E-mail: Office Hours: | Instructor: Dr. Cherie YestrebskyOffice: CH 325Phone: 407-823-2135E-mail: cherie.yestrebsky@ucf.edu | University Course Catalog Description Illustration of chemical principles and introduction to the techniques of inorganic and physical chemistry. Course Overview The Chemistry Fundamentals Laboratory course is designed to give students an insight into the processes of experimental chemistry. The course provides a series of authentic, challenging, and relevant questions which students seek to answer through experimentation in a safe environment. Lab techniques like titration, spectroscopy, dilution, and measurement will be incorporated with critical thinking exercises to enhance the learning process and improve comprehension of fundamental concepts. An introduction to writing within the science discipline will be addressed each week. Course Objectives At the end of the semester, students will be able to: * Keep safety the first priority while working in the laboratory * Design a procedure to answer a key question * Model how writing is used in a variety of chemistry genres * Experiment with glassware & equipment in alignment with their intended function * Develop stronger critical thinking skills * Use laboratory terminology/vocabulary in text and...
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...Michaelis-Menten Kinetics: The Enzyme-Catalyzed Hydrolysis of Sucrose1 Background Of the numerous chemical reactions whose kinetics have been studied, some of the most interesting and complex are those which take place in living organisms. These reactions, when studied under non-biological conditions (lack of organisms and their various biomolecules), are often exceptionally slow: often hours or days, and sometimes even years are required before equilibrium is reached. Yet life requires seconds or even fractions of seconds to produce the same results, and it does this through the use of biological catalysts called enzymes. Enzymes are proteins with special clefts or pockets in their structures in which the reactions occur: the tremendous advantage that enzymes have is that these pockets, by nature of their shape and chemical environment, force the reactants to form transition states much lower in energy than the transitions states the reactants would form under other circumstances, say in solution. Thus, life can subsist at a reasonable pace and it does not take us a week to walk across a room or digest an apple. Of the many kinetic models for enzymes currently used, the first (and simplest) developed is the Michaelis-Menten Model, developed in 1913 by Leonor Michaelis and Maud Menten, which is still quite useful today. One of its early triumphs was the successful explanation of the kinetics of sucrase, or β-fructofuranidase, an enzyme found in yeast which cleaves...
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...and Guns The world of Forensic Scientist is an amazing and fascinating place. There are so many aspects that go into forensic science but in this paper we are only covering bloodstain spatter patterns. Bloodstain spatter patterns are not solely used to solve crimes but I do feel it is one of the most important. Bloodstains never lie. A bullet is traveling at its fastest speed when it leaves the barrel of the gun. Bullets traveling after firing is said to be using kinetic energy. Kinetic energy is the energy the bullet has due to its motion from the firing pin striking the gun powder causing an explosion that forced the bullet to discharge from the barrel. Now this bullet possess kinetic energy and will do harm to anything lying within its targeted path. (Kinetic Energy website) The kinetic energy given to bullets fried from rifles will be a higher velocity then that of a pistol or shot gun. Rifles barrels are longer and constructed to with stand more pressure and thus leading to a greater velocity of kinetic energy on a bullet (Warlow 67). Death can occur from firing a bullet straight up in the air. When the bullet is returning to the earth due to the gravitational pull it will pick up velocity. However the bullet can return either base or head side up (89). Powders also have different burning rates for different guns. When you have a longer barrel your burning rate of powder will be longer but if your barrel is shorter then you will have a faster burning rate (Warlow 67)...
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...Osmosis of Water Biology 105 Lab 3/22/11 Introduction Cells have kinetic energy, a source of energy stored in cells. This energy causes molecules to bump into each other and move in new directions. Diffusion is one result of this molecular movement. Diffusion is the random movement of molecules from an area of higher concentration to areas of lower concentration. Osmosis is a special kind of diffusion where water moves through a selectively permeable membrane that only allows certain molecules to diffuse though (Lab Manual 7e, 2010). Diffusion or osmosis occurs until dynamic equilibrium has been reached. This is the point where the concentrations in both areas are equal and no net movement will occur from one area to another. If two solutions have the same solute concentration, the solutions are said to be isotonic. If the solutions differ in concentration, the area with the higher solute concentration is hypertonic and the area with the lower solute concentration is hypotonic. Besides osmosis and diffusion, molecules and ions can be moved by active transport. This process includes the use of ATP (adenosine tri-phosphate) to drive molecules in or out of a cell. Active transport is generally used to move molecules against a concentration gradient, from an area of low concentration to an area of higher concentration of molecules. The purpose of this experiment was to show that osmosis of water thru plant stomata does not require energy (ATP)...
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...requiring the same building blocks for life as organisms inhabiting moderate (mesophilic) temperatures. Despite this, research on the biosynthetic pathways psychrophiles use is very limited. The enzymes found in these microbes are adapted with structural features that give them the ability to function up to 10 times higher catalytic efficiency than their mesophilic homologues. The overall goal of the proposed research is to use a biochemical and genetics approach to study the stability, structure, and function of psychrophilic proteins involved or believed to be involved in the nitrogen metabolism of Methanococcoides burtonii. The specific objectives are: 1. Investigate the structural and functional properties of M. burtonii’s nifH and nifD gene products using sequence analysis, UV-VIS spectroscopy, and two-hybrid studies; 2. Investigate the structure, stability, and kinetics of M. burtonii’s glutamate dehydrogenase through sequence analysis, site-directed mutagenesis, protein modeling, and kinetic assays. Cold-enzyme study can increase knowledge in the field of protein folding and catalysis and the broader impacts of this project include (1) potential biotechnological applications of cold-enzymes (2) the involvement of high school students and science teacher(s) in hands-on research; and (3) the presentation of research results to a non-scientific audience in understandable terms. Project Description Research Objectives and Significance About 75% of our biosphere’s temperature...
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...A Kinetics Experiment The Rate of a Chemical Reaction: A Clock Reaction Andrea Deoudes February 2, 2010 Introduction: The rates of chemical reactions and the ability to control those rates are crucial aspects of life. Chemical kinetics is the study of the rates at which chemical reactions occur, the factors that affect the speed of reactions, and the mechanisms by which reactions proceed. The reaction rate depends on the reactants, the concentrations of the reactants, the temperature at which the reaction takes place, and any catalysts or inhibitors that affect the reaction. If a chemical reaction has a fast rate, a large portion of the molecules react to form products in a given time period. If a chemical reaction has a slow rate, a small portion of molecules react to form products in a given time period. This experiment studied the kinetics of a reaction between an iodide ion (I-1) and a peroxydisulfate ion (S2O8-2) in the first reaction: 2I-1 + S2O8-2 I2 + 2SO4-2. This is a relatively slow reaction. The reaction rate is dependent on the concentrations of the reactants, following the rate law: Rate = k[I-1]m[S2O8-2]n. In order to study the kinetics of this reaction, or any reaction, there must be an experimental way to measure the concentration of at least one of the reactants or products as a function of time. This was done in this experiment using a second reaction, 2S2O3-2 + I2 S4O6-2 + 2I-1, which occurred simultaneously with the reaction under investigation. Adding...
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...Introduction: In this experiment, the rate law and rate constant for a reaction of oxalic acid with permanganate were determined. Every chemical reaction ranges from hours to femtoseconds to occur. The rate of a chemical reaction can be determined by assessing the change in either the reactant or product in a given time or point (Connors, 1990). It is important to take account of the stoichiometric ratios of each component, regardless as to which compound is chosen to determine the rate. The study of the rates of chemical reactions is called Chemical Kinetics (Soustelle, 2011). The following is an example of a generic chemical reaction: aA + bB → cC + dD The rate of the reaction can be expressed as a function of each reactant and each product: Rate = -1a∆A∆t= -1b∆B∆t= +1c∆C∆t= +1d∆D∆t For each reaction, the concentration is decreasing as the reaction occurs; therefore representing the rate as a positive value and a negative sign in the rate definition is used. The concentration of the products is conversely increasing as the reaction proceeds; therefore changing in concentration is positive (Connors, 1990). The reaction rate can be measured by accurately measuring the change in concentration of one of the reaction species over time. This experiment utilized a visual change in color of one of the reactants used; however, since visual indications can be subjective and it is important to perform the experiment multiple times. Also, since the color change would indicate that...
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...Strong’s topics are presented in updated form. Today we are blessed with UHV compatible materials, advanced fabrication methods, standardized fittings and a plethora of pumps and gauges, almost all of which are available for purchase via the internet. We forget about the days when obtaining a decent vacuum involved a certain resolve and ability to make do with adapted components and various concoctions of sticky goops. In this article we’ll take a look at some of the examples in Strong’s handbook and compare them to what we have now. Strong’s RepresentativeVacuum Systems Strong divided vacuum systems into two classifications: static and kinetic. Static system were of the type that would be used for achieving high levels of clean vacuum for applications such as evacuating x-ray tubes and surface studies. Kinetic systems were of the type that could be opened frequently for process type work and where small leaks and higher outgassing rates could be tolerated. While we don’t use those terms today, the...
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