...Both ethanol and caffeine are present in many consumable goods such as beers and coffee (Lab Notebook). Both drugs are known for causing physiological differences in organisms. It is important to be aware of the potential ramifications these substances since they are so widely used and ingested. In humans, heart rates were increased slightly with the additional of ethanol (Abdel-Rahman 1987). In rats, heart rates were both increased and decreased with the addition of ethanol depending on the level of stress the rats were in (Arciero 1998). In humans, caffeine did not affect heart rates (Sparrow 1987). Different ages of humans had heart rates that were not affected by caffeine (Umemura 2006). The purpose of this experiment was to test if the quantity of ethanol and caffeine increases or decreases the heart rates in Daphnia magna. We predicted that the heart rates of the Daphnia would rise when exposed to ethanol, yet would remain the same when exposed to caffeine. We also hypothesized that the higher the concentration of the drug in the solution, the more effect the drug would have on the organism. Methods We conducted the experiment on Daphnia...
Words: 537 - Pages: 3
...Kaitlyn Hainline Biology-4 The Cell Cycle Lab I. Design A. Problem/Research Questions 1. What is the relative time it takes for a cell to go through each phase? B. Variables 1. Independent Variable- The independent variable is the different cell phases (Interphase, Prophase, Anaphase, and Telophase/Cytokinesis). 2. Dependent Variable-The dependent variable is the percentage of cells that are each cell phase. C. Controlled Factors- The magnification is a control factor because I was able to decide what magnification the microscope was on and mine was at (400x). Another control factor was the temperature because it stayed at room temperature throughout the whole experiment. D. Apparatus/Materials Needed * 2 pre stained onion root slides * A microscope at 400x magnification * Calculator * writing utensil * paper E. Procedure 1. Observe every cell in one high-power field of view and determine which phase of the cell cycle the cell is in. 2. Count at least two full fields of view. If you have not counted at least 200 cells, then count a third field of view. 3. Record, process and present your data. II. Data Collection and Processing A. Data Collection 1. Mass of | Number of cells in each phase + 1cell | Percent of cells in each phase (%) | Time spent in each phase (hours) | 2 slides tested | Slide 1 | Slide 2 | Slide 1 | Slide 2 | Slide 1 | Slide 2 | Interphase | 75 | 215 | 32% | 77% |...
Words: 1092 - Pages: 5
...Atichai Suwannapeng Pre Lab #6 Diffusion, Osmosis, and Tonicity Group #3 Section A3 Group Members: Laghu Shakya, Alex Maican, Kelvin Chen, Ziye Lin 10/18/2015 Abstract: In this lab we will be gaining an understanding of how transport in membranes work. This is important because in our semi permeable cell membrane the mode of movement relies on transport. Some methods of transportation for molecules are simple diffusion, facilitated diffusion, active transport, exocytosis and osmosis. The reason molecules tend to move around when dissolved in a solution is because all molecules display random thermal motion and have kinetic energy. Kinetic energy is what allows the molecules to diffuse down a gradient of high concentration to regions of low concentration until the distribution of molecules become equal and achieved dynamic equilibrium. The entire solution only becomes homogeneous when one of the several factors are reached: the size of the dye molecules, temperature of the solution, density of the solvent and concentration of the dye. Heat is what causes random motion of molecules and passively moves molecules in biological systems. However, we can’t see this movement with our naked eye. In order for us to see this movement, we must use a microscope to see the small particles move after collision, this is called the Brownian movement. When talking about the cell membrane we must understand that it is selectively permeable, which means it can choose what can pass through...
Words: 1835 - Pages: 8
...09-2.01 Read the entire experiment and organize time, materials, and work space before beginning. Remember to review the safety sections and wear goggles when working with chemicals. Objectives: To learn flagellar structure and arrangements common in microbes, and To use direct observation and testing to determine if a given microbe is motile. Materials From: Label or Box/Bag: Qty Item Description: Student Provides 1 Microscope 1 Paper Clip 1 10%-bleach Solution 1 Paper towels From LabPaq 1 Gloves packages - 11 pairs 1 Immersion Oil 1 Slide - Cover Glass - Cover Slip Cube (4) Culture Media Bag #2 - Refrigerate upon Receipt Culture Media Bag #2 - Refrigerate upon Receipt 2 Agar, 0.4% Motility Test Agar - 8 mL in Glass Tube Inoculation Instruments Inoculation Instruments 1 Inoculation Loop, Plastic Mask Bag Mask Bag 1 Mask with Earloops (11) in Bag 5" x 8" Slide Box MBK Slide Box MBK 1 Slide-Box-MBK with Blank-Slides (4) Pre-Lab Preparation: Place saved cultures of E. coli and S. epidermidis (from previous lab) in incubator 12-24 hours prior to the start of the experiment. Discussion and Review: Many bacteria are capable of motility, the ability to move under their own power. Most motile bacteria propel themselves by special organelles termed flagella. The bacterial flagellum is a noncontractile, semi-rigid, helical tube composed of protein and anchors to the bacterial cytoplasmic membrane and cell well by means of disk-like structures...
Words: 1250 - Pages: 5
...[pic] BSC2085L HUMAN ANATOMY & PHYSIOLOGY I LAB SYLLABUS Term 20151 Session 1 INSTRUCTOR: Dr. Mizanur Rahman OFFICE: Bldg. 72/ 2nd Floor (Academic Success Center) OFFICE DAY/TIME: Monday: 10:45 am -11.45 am/ Tuesday: 6 - 7 pm (Academic Success Center/Bldg.72/2nd Floor) TELEPHONE: 954-529-7195 (Prefer email than call me) EMERGENCY HOTLINE: 954-201-4900 (For school open/close info) CLASS ROOM: 70/116 CLASS DAY/HOUR: Monday: 12 - 1:50 pm (Ref.# 499260) Tuesday: 8 - 9:50 am (Ref.# 494608) Tuesday: 10 - 11:50 am (Ref.# 494609) Tuesday: 4 - 5:50 pm (Ref.# 494616) E-MAIL: mrahman@broward.edu PRE-REQUISITES: BSC2085 (Human Anatomy and Physiology I) CO-REQUISITES: BSC2085L TEXT: Exploring Anatomy & Physiology in the Laboratory / By Erin C. Amerman, 2013 (2nd Edition), ISBN-13: 978-161731-056-0) COURSE DESCRIPTION: This is a laboratory section that runs in conjunction with the Human Anatomy and Physiology I (BSC...
Words: 1988 - Pages: 8
...Demonstrate proper techniques involved in using centrifuge * Using filtration and centrifugation, separate the chloroplast from other organelles in a spinach leaf * Identify mitochondrion in an onion cell Materials: * Fresh spinach leaves * Grinding solution * 0.33 M sorbitol * 10mM sodium pyrophosphate (NaPO) * 4mMMgCl * 2mM Ascorbic Acid * Adjust pH to 6.5 with HCl * Chopping board and knife * Chilled mortar and pestle * Cheesecloth * Refrigerated preparative centrifuge * Suspension solution * 0.33 M Sorbitol * 2mMEDTA * 1rnMMgCl * 50rnMHEPES * Adjust pH to 7.6 with NaOH * Hematocytometer and microscope * Ice bath * Sprig of elodea Procedures: Part 1: Choroplasts from Spinach Leaves 1. Prepare an ice bath and pre-cool all glassware to be used. 2. Select several fresh spinach leaves and remove the large veins by tearing them loose from the leaves. Weigh out 4.0 grams of...
Words: 1049 - Pages: 5
...4- pre lab: preparation for protocol Investigating: Neurospora Crassa Hyphal Tip Growth. Hypothesis: Null Hypothesis | Another Hypothesis | 400 nm of Nacodazole will have no effect on Neurospora Crassa Hyphal Tip Growth. | 400 nm of Nacodazole will have an effect on Neurospora Crassa Hyphal Tip Growth. | 400 nm of Nacodazole will have no effect on Neurospora Crassa Hyphal Nuclear position. | 400 nm of Nacodazole will have an effect on Neurospora Crassa Hyphal Nuclear position. | Variables: Fungal growth Tip | Nuclear positioning | Independent on time | Independent on time | Dependent on the distance migrated | Dependent on the distance migrated | Controls: * Observing the normal movement of Neurospora Crassa Hyphal Tip Growth without 400nm of Nacodazole. * Observing the normal Nuclear position of Neurospora Crassa Hyphal Tip Growth without 400nm of Nacodazole. Protocol : Investigation of Neurospora Crassa fungal tip growth and nuclear position. 1) From day 3, Neurospora Crassa cushions slides are required 2) Kohler Illumination set up under bright field microscopy. 3) Pick up two agar cushions without bubbles and label them as control 1 and control 2 4) Add 50 ul of growing medium to each control and place a covering slips on the. 5) For 5 minutes, put the slides inside the incubator allowing the medium to transfer into the cell 6) After, place the one of the control under kohler Illumination microscope and...
Words: 786 - Pages: 4
...negative. The Gram-positive bacteria have an intricate set of amino sugars which we call peptidoglycan that form a thick cell wall around the plasma membrane. On the other hand, Gram-negative bacteria have an uncomplicated cell wall which thus has less peptidoglycan. The main source of identification within the Gram test is the fact that Gram-positive bacteria appear blue-purple while Gram-negative bacteria appear pink (Gram Stain). Bacteria can come in one of three different shapes; spirilla (spiral-shaped), cocci (round-shaped), and bacilli (rod-shaped). In order to be able to see these shapes a bacteria must be stained or dyed. I hypothesis that we will have one Gram-positive culture and one Gram-negative culture. (Lab Manual) In order to prepare this lab experiment we performed five steps. The first step in preparing this experiment was to heat fix a bacteria culture sample which in return stopped the sample from coming off the slide during the experiment. In order to perform this first step we first turned on a Bunsen burner and set the flames to a blue color which was used to sterilize an inoculating loop. Next, a drop of water was added to the slide using the loop which was again sterilized via the Bunsen burner. After, bacteria was collected from a culture tube and smeared onto the slide. We spread the bacteria onto the slide until we reached a milky, pale appearance. After we smeared the bacteria we allowed it to air dry and then passed it through the Bunsen burner...
Words: 1125 - Pages: 5
...Lab Manual Introductory Biology (Version 1.4) © 2013 eScience Labs, LLC All rights reserved www.esciencelabs.com • 888.375.5487 2 Table of Contents: Introduc on: Lab 1: Lab 2: Lab 3: Lab 4: The Scien fic Method Wri ng a Lab Report Data Measurement Introduc on to the Microscope Biological Processes: Lab 5: Lab 6: Lab 7: Lab 8: Lab 9: The Chemistry of Life Diffusion Osmosis Respira on Enzymes The Cell: Lab 10: Lab 11: Lab 12: Lab 13: Lab 14: Lab 15: Cell Structure & Func on Mitosis Meiosis DNA & RNA Mendelian Gene cs Popula on Gene cs 3 4 Lab Safety Always follow the instruc ons in your laboratory manual and these general rules: eScience Labs, LLC. designs every kit with safety as our top priority. Nonetheless, these are science kits and contain items which must be handled with care. Safety in the laboratory always comes first! Lab Prepara on • • Please thoroughly read the lab exercise before star ng! If you have any doubt as to what you are supposed to be doing and how to do it safely, please STOP and then: Double-check the manual instruc ons. Check www.esciencelabs.com for updates and ps. Contact us for technical support by phone at 1-888-ESL-Kits (1-888-375-5487) or by email at Help@esciencelabs.com. • Read and understand all labels on chemicals. If you have any ques ons or concerns, refer to the Material Safely Data Sheets (MSDS) available at www.esciencelabs.com. The MSDS lists the dangers, storage requirements, exposure treatment...
Words: 26048 - Pages: 105
...This experiment will be done using C57BI/6 wild type mice from Professor Moore’s lab. To begin two dams will be mated per male sire over night for a total of 10 females with 5 males by 5 pm. We will then look for evidence of fertilization through identification of the post copulation vaginal plug by 10:00 am the next morning. If more than two copulation plugs are identified then we will increase the number of dams per treatment group starting by increasing the number receiving lithium carbonate injections, but still attempting to maintain the 1:1 ratio of saline injected to lithium carbonate injected mice. The animals will be housed in specific pathogen-free barrier housing in the basement of LSP. It is likely that two rounds of timed matings...
Words: 684 - Pages: 3
...Methyl Red Voges-Proskauer Test Lab Tetia Richardson Microbiology DL3: Instructor Newton 3/29/2015 PURPOSE This experiment is designed to become familiar with and perform the MR-VP biochemical test, learn variations in how different organisms metabolize glucose, and to become familiar with and perform the catalase biochemical test. Materials Used 10% bleach solution Hydrogen peroxide Paper towels Saved E. coli culture Stock culture: S. epidermidis Gloves Candle used for a flame source Test Tube Test Tube rack Pipet Slide-Box-MBK with blank slides 2 Broth, MR-VP - 5 mL 1 Barritt’s A Reagent - 3 mL in Pipet 1 Barritt’s B Reagent - 3 mL in Pipet Methyl Red Reagent, 0.1% - 1 mL in Pipet 1 Inoculation Loop, Plastic 1 Mask with Earloops PROCEDURE Exercise 1 Procedural Steps The saved E. coli culture and S. epidermidis stock culture was incubated 12-24 hours prior to the start of the experiment. The work area was disinfected with 10% bleach solution. The MR-VP tubes were labeled: one E coli and the other S epidermidis. Each MR-VP broth tube was inoculated with the corresponding organism using aseptic techniques. The tubes were incubated for 48 hours at 35oC-37oC The reagents were allowed to warm to room temperature Two test tubes were labeled E. coli and two test tubes were labeled S. Epidermidis Half (2.5 mL) of the incubated MR-VP broth labeled E. coli was transferred into the two corresponding test tubes. This was repeated for the...
Words: 1338 - Pages: 6
...INSTRUCTOR GUIDE Human Anatomy & Physiology Laboratory Manual MAIN VERSION, Eighth Edition Update CAT VERSION, Ninth Edition Update FETAL PIG VERSION, Ninth Edition Update ELAINE N. MARIEB, R.N., Ph.D Holyoke Community College SUSAN T. BAXLEY, M.A. Troy University, Montgomery Campus NANCY G. KINCAID, Ph.D Troy University, Montgomery Campus PhysioEx™ Exercises authored by Peter Z. Zao, North Idaho College Timothy Stabler, Indiana University Northwest Lori Smith, American River College Greta Peterson, Middlesex Community College Andrew Lokuta, University of Wisconsin—Madison San Francisco • Boston • New York Cape Town • Hong Kong • London • Madrid • Mexico City Montreal • Munich • Paris • Singapore • Sydney • Tokyo • Toronto Editor-in-Chief: Serina Beauparlant Project Editor: Sabrina Larson PhysioEx Project Editor: Erik Fortier Editorial Assistant: Nicole Graziano Managing Editor: Wendy Earl Production Editor: Leslie Austin Composition: Cecelia G. Morales Cover Design: Riezebos Holzbaur Design Group Senior Manufacturing Buyer: Stacey Weinberger Marketing Manager: Gordon Lee Copyright © 2009 Pearson Education, Inc., publishing as Pearson Benjamin Cummings, 1301 Sansome St., San Francisco, CA 94111. All rights reserved. Manufactured in the United States of America. This publication is protected by Copyright and permission should be obtained from the publisher prior to any prohibited reproduction, storage in a retrieval system, or transmission in any form or by any means...
Words: 120457 - Pages: 482
...twelfth; seven students are below grade level and 22 students are at or above grade level. Based on District Star Assessment results, two students are receiving additional reading help and one students is in a block math, and three students have specialized study halls. Interventions in the district are block classes co-taught with a special education teacher, remedial reading classes, or smaller more supportive study halls. This class has many hard working students that ask questions and have good rapport with each other. During many of the assignments and labs, the students worked collaboratively with their shoulder partner. This unit had aspects that were challenging for the students. Higher level thinking was demonstrated often with an analogy and writing conclusions and summaries of activities and labs. To help the students, assignments and rubrics were uploaded to Schoology, an online learning management system. All lab space is shared, so planning with the other Biology teachers was necessary so classes didn’t overlap. The high school is not one to one with technology, so computers need to be checked out. One student in class did not have access to computers at home, so it was necessary to make sure she had access to all assignments will she was at school. 2. Planning Instruction: Goals for this unit were to show that the process of structure determines function starts in cells and this process enables living things to maintain a balanced equilibrium, homeostasis...
Words: 4614 - Pages: 19
...Name: Your student Number: Faculty of Science and Engineering Faculty of Science and Engineering Practical Skills Practical Skills 4AB012/4BM005/4BM013/4PY008 Practical Session Laboratory Hand Book Basic Microbiology 2013-2014 Practical Sessions 1 – 5 Welcome and Some notes about this Hand book This booklet is your guide to the next 5 practical sessions. It contains all the methods that you need to complete each of the experiments you will carry out step by step. You must refer to these methods during the sessions as the ability to follow a standard method carefully and accurately is an essential skill that any scientist must learn. Other than the various methods the booklet also contains space to record the data you will gather during each of the sessions. This will be used later to share with the group so it is essential that you accurately and neatly complete the tables or write in the spaces provided without doodling or scribbling on the pages. You will also notice there may be a series of formative questions following each section. These will help you consolidate your knowledge and some preparation towards your final course work submission and exam. Finally, keep this book clean and tidy and it will be an invaluable source of information for the next few years on your degree course. We hope you enjoy the practical sessions after all it’s what science is all about. All the best The Module team xxx ...
Words: 13031 - Pages: 53
...The topic of Mars has long been of interest to astronomers and science fiction enthusiast alike. The premise of another planet supporting life excites people like no other. In 2004, The United States National Aeronautics and Space Administration, or NASA, began preliminary science experiments and instrument proposals for the Mars Science Laboratory (MSL) and a robotic space probe mission to Mars. After long testing and development stages, the mission birthed a rover, Curiosity, which was launched in November 2011 and subsequently landed August 6th 2012. As we speak Curiosity is collecting invaluable data for our understanding of mars including: habitability, climate and geology, and possibly setting up a manned mission to mars in the future. The possibilities that this new information can bring are the main reason that scientist and nonscientists alike are so excited for this pivotal mission. The Curiosity project began development in 2004. Astronomers and engineers worldwide entered their instrument proposals to NASA so they could hopefully be a part of the final mission. These components were sifted thoroughly and select components were developed for four years. By 2008, they were mostly finished with the hardware and software developments and they carried on testing. This extensive testing delayed liftoff, which was originally slated for September 2009, until November 2011. NASA then administered a poll on their website to decide the name of the rover, with Curiosity ultimately...
Words: 1255 - Pages: 6
