...announcement of what the business does today and why it exists. A Mission Statement captures the uniqueness of theorganization and its purpose or reason for existing (Evans & Lindsay, 2011). Strategies are broad statements that set the direction for the organization and state how the organization intends to achieve its mission and vision. Strategic objectives are important to an organization if it is to remain competitive (Biznik, 2012). 2. Statements a. History. Beckman Coulter began its history as two separate companies; Beckman Instruments and Coulter Electronics. Beckman Instruments was established by Dr. Arnold O. Beckman who invented a way for determining the precise measurement of pH in lemon juice which he called the acidometer, or more commonly known as the pH meter. Separately, the Coulter brothers, Wallace and Joseph, had invented an instrument that could measure cell size through electrical impedance- known today as the Coulter Principle. Coulter Electronics was acquired by Beckman Instruments in 1997 and the new organization was called Beckman-Coulter, Inc. The newly formed company covers the full spectrum of biomedical instrumentation for both the diagnostic and the research markets including hematology, cytometry, lab automation, centrifugation, immunochemistry, and a series of Life Science products such as...
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...microscope at first, and then use the fine to get the image in perfect focus. Always use the only fine adjustment when using the high powered lens. 4. Always put the dust back on the microscope when you are not using it. Instrument: pH meter - A pH Meter is a device used for measuring the pH, which is either the concentration or the activity of hydrogen ions, of an aqueous solution. It usually has a glass electrode plus a calomel reference electrode, or a combination electrode. pH meters are usually used to measure the pH of liquids, though special probes are sometimes used to measure the pH of semi-solid substances. Instructions on using and calibrating Ph meter: 1. Make sure that the pH probe you are using has been stored in a storage solution or a pH 4 solution. If this is not the case, soak the probe in distilled water for at least 24 hours. 2. Check that the meter is set in pH mode, and then rinse the probe of your meter in distilled water. Shake it off before placing it in a pH 7 solution for calibration. 3. Let the probe remain in the solution for at least 30 seconds to allow time for the meter to stabilise, and then adjust the meter so that it reads pH 7. 4. Rinse once again and then place it into a pH 4 solution, giving time for the meter reading to...
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...TechPro™ Operation Manual Model ARH1 MYRON L COMPANY 10-02 (WEB) EG Instrument Illustration Reference Junction (under Glass Bulb) pH Sensor (User replaceable) Conductivity Cell (Built-in Electrodes) Temperature Sensor pH Glass Electrode pH Sensor Protective Cap User selectable temperature compensation ratios (Solution Selection) KCl NaCl 442 Units of Measurement Parameter LO BATT mS µS PPM PPT mS - millisiemens/cm (millimhos/cm) µS - microsiemens/cm (micromhos/cm) PPM - parts per million PPT - parts per thousand Conductivity Conductivity TDS TDS Icon for pH COND pH °C TDS Icons for either Conductivity or Total Dissolved Solids (TDS) COND TDS pH pH key Conductivity or TDS key: User selectable for either Conductivity or TDS (Switch Selectable) pH/CONDUCTIVITY METER ARH1 MYRON L COMPANY Wrist/neck strap slot (user supplied) For detailed explanations, see Table of Contents 1-12-99 1 FEATURES and SPECIFICATIONS • • • • • • • • • • • A. Features Superior resolution 3 1/2 digit LCD Conductivity/TDS accuracy of ±1% of full scale pH accuracy of ± .05 pH units All electrodes are internal for maximum protection Latest electrode cell technology Water resistant Autoranging Conductivity/TDS Easy Conductivity/TDS and pH calibration User selectable Conductivity/TDS modes 3 “User Selectable” solution conversions (tempcos) Temperature Accuracy of ±1° C/F 3 1/2 Digit LCD 7.7x2.7x2.5 in. 196x68x64 mm 11.2oz./320g ABS ABS 0.2 oz./5 ml 0.04...
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...there is a significant amount of base (OH-) in the solution. In this lab experiment, the rate of this reaction will be studied using an ester called para-nitrophenyl acetate (PNA), which produces an alcohol, para-nitrophenol (PNP). Question: What is the rate of reaction for the hydrolysis of PNA? What is the rate constant k? How are the rate of reaction and the rate constant k affected by varying (1) substrate PNA concentration, and (2) changes in pH (OH- base concentration) and addition of different nitrogen-containing base compounds (i.e.catalysts)? Hypothesis: I hypothesize that the rate of reaction and the rate constant for the hydrolysis of PNA can be determined experimentally to be first order. Also, in the reaction, the experiment will develop as follows: PNA +H2O --> PNP (yellow) + Ac Materials and Methods: The following solutions will be used in the experiment. 1. 0.2 M Phosphate Buffer, pH 6.5 (13.6 g KH2PO4 / 0.5 L, adjust to pH 6.5) 2. 0.4 M Phosphate Buffer, pH 6.5 (27.2 g KH2PO4 / 0.5 L, adjust to pH 6.5) 3. 2 M Potassium Hydroxide (11.2 g KOH / 100 ml solution) 4. 4(para)-nitrophenyl acetate (PNA), 0.0005 M (0.045 g /500 ml), store in 4 oC fridge. 5. Imidazole, 0.025 M (0.17 g Imidazole /100 ml); 6. Super 10x Imidazole, 0.25M (1.7 g /100 ml) 7. 2-Methylimidazole, 0.025 M (0.21 g...
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...strips of paper known as pH paper for very broad measurements of pH. pH paper can be bought for acidic neutral and alkaline compounds. Once dipped into the hair product, the paper changes color depending on the pH of the product. Then the color can be matched to a key (included with the pH paper) to determine the approximate pH. 2 Use a digital pH meter for more precise measurements. Though the specifics of using a pH meter will vary according to the model, the basics are the same. Sponsored Links Litmus paper Red, blue and neutral paper in books and reels manufactured in UK www.kaagat.com 3 Wash the electrode. The electrode is usually encased in a glass tube and can be washed by squirting it with distilled water. 4 Prepare the machine for calibration. This can be done by pressing the "cal" button on the machine and then immersing the electrode in one of the calibration solutions provided with the machine. These solutions are generally supplied at pH levels of 4, 7 and 10, and are in specially prepared bottles. The pH meter must be calibrated in two different solutions, but it doesn't matter which two are used. 5 Continue with the calibration. After pressing the "Cal" button, immerse the electrode in one calibration solution. Once the readout on the digital screen stabilizes, press the button marked "OK." Repeat this using a different solution (for example, calibrate first with the pH 7 solution, then calibrate again with the pH 10 solution). 6 Wash the...
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...Introduction Buffer is a solution that resists a change in pH when bases or acid are added. Solutions that are acidic contain high concentrations of hydrogen ions (H+) and have pH values less than seven. Buffer usually consist of a weak acid, and its conjugate base or a weak base and its conjugate acid. The function of buffer is to resist the changes in hydrogen ion concentration as a result of internal and environmental factor. This buffer experiment is important so that we relies the important of buffer in our life. Besides that, it is also important so that we master the buffer preparation techniques and can conduct the experiment ourselves without anyone to instruct us. Other than that, we got to learn how to operate a pH meter and we...
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...Acids, Bases, PH Introduction. This experiment helped to understand difference between acid and base and there PH levels. We learned how to identify buffer. Buffer is substances that resist changes in PH when acid or base is added to solution. Also we learned that water is neutral. Its mean that water has an equal concentration of H and OH. PH number of water is 7. Acid’s PH numbers less than 7. Bases’ PH number is greater than 7. As well we learned how to use and calibrate PH Meter. Materials and methods. For experiment at class we used two solutions, solution A and solution B. First we took PH meter and measured PH level of solution A. After we add to solution A 1.0ml of HCl and record PH level. Total of HCl added was 10 ml, we did it by adding 1.0 ml of HCl at the time. Second time we took solution A and start adding 10 ml(1.0 ml at the time) of NaOH. With PH meter we record number changing in PH. Third time we took solution B , measured PH level and did the same steps as with solution A. Results. Solution A. ml of acid or base added 1 2 3 4 5 6 7 8 9 10 HCl 6.95 6.91 6.92 6.93 6.91 6.90 6.88 6.88 6.87 6.88 NaOH 6.99 6.99 7.02 7.01 7.02 7.01 7.02 7.03 7.06 7.07 Solution B. ml of acid or base added...
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...compounds. Many compounds can look very similar, but have drastically different chemical properties, so it is important to know which tests can be performed on a compound to help determine what it is, or what it is not. In this experiment 2 sets of six unknown solid compounds were put into small jars and labeled with the numbers 1-12, numbers 4, 6 and 8 were not used. Each lab group was given one unknown solid and told to determine the samples properties through various testing. Physical characteristics were observed, by using a magnifying glass to view a small amount of the sample. Relative solubility was found by mixing a small amount of the unknown solid with deionized water. Electrical conductivity was measured by using a conductivity meter and noting the amount of bars on the...
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... Acid-Base Reactions Purpose: The purpose of this set of laboratory experiments is to study the correlation between acids and bases by measuring and comparing their pH level. This can then be used to further evaluate the neutralization of acids with a base, such as stomach acid with calcium carbonate, antacid tablets. Data: Part B-1: Test pH of Laboratory Chemical Solutions Name Chemical Formula Calculated pH of 0.010 M Solution Laboratory pH Test results (0.010 M) Sodium Chloride NaCl 7 7.07 Ammonia 〖NH〗_3 10.62 10.15 Sodium Acetate 〖NaCH〗_3 〖CO〗_2 8.88 7.24 Hydrochloric Acid HCl 2.0 1.71 Ammonium Chloride 〖NH〗_4 Cl 5.63 6.58 Acetic Acid 〖CH〗_3 〖CO〗_2 H 3.38 3.33 Sodium Hydroxide, Lye, or Caustic Soda NaOH 12.0 11.68 Ammonium Acetate 〖NH〗_4 〖CH〗_3 〖CO〗_2 7.0 7.69 Part B-2: Test pH of paired Acid and Base solutions: Compound Names Compound Formulas Measured pH of Equal volumes of Solutions Hydrochloric Acid + Sodium Hydroxide HCl+NaOH 4.58 Ammonia + Acetic Acid 〖NH〗_3+〖CH〗_3 〖CO〗_2 H 9.05 Part B-3: Test pH of common Household items Household Item Name: Test pH: Lemon Juice 2.07 Ammonia 11.53 Mouth Wash 4.04 Vinegar 2.02 Part C- Testing Effectiveness of Antacids Acid Solution Antacid Test #1 pH result Test #2 pH result Diluted 20 mL of HCl solution to simulate Stomach Acidity none 2.45 Control Value 2.45 Control Value Diluted 20 mL of HCl solution to simulate Stomach Acidity 0.20 grams of Topcare Brand...
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...Lynne Andrew NB 1110 / M 5 – 7:50 Lab 1 February 2, 2015 pH and Buffer – Using Red Cabbage Indicator to Measure pH Introduction This experiment was performed to evaluate the following hypothesis: Red cabbage indicator can be used as a measure of pH. According to Campbell, et al, in Biology: Concepts and Connections, red cabbage contains a water-soluble pigment called anthocyanin that changes color when it is mixed with an acid or a base. The pigment turns red in acidic environments with a pH less than 7 and the pigment turns bluish-green in alkaline (basic) environments with a pH greater than 7 (2012). The pH indicating property of red cabbage indicator is well accepted in the scientific community and is a useful standard for us to become familiar with. As stated in the lab text, having a known set of standards is important to gaining information about unknowns (2011). Red cabbage contains high levels of anthocyanins, which give the cabbage its deep purple hue. The pigments also change in relation to changes in pH, according to the lab text (2011). According to a University College London lab experiment titled Red Cabbage pH Indicator, with most pH indicators, the compound gains a proton at low pH (lots of H+) but loses it at higher pH. Although this may seem a slight change, it is enough to alter the wavelengths of light reflected by the compound. As a result, we see the color change with respect to pH. The lab description continues to state anthocyanins have...
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...Potentiometric Measurement – Determination of the pH of an alkali solution, a buffer solution , a hair shampoo, a hand wash and distilled water. Objective To carry out potentiometric measurement to determine whether a solution is controlled by the buffer system or by a strong electrolyte. To prepare the samples with different concentration by adding distilled water. Introduction The potentiometric measurement is to determine the difference in potential between a working (an indicator) electrode and a counter (reference) electrode by using the potentiometer. The working or indicator electrode represents the cathode which locates at the right half-cell whereas the counter or reference electrode represents the cathode which locate at the left half-cell. In most of the chemical processes, pH is one of the most general laboratory measurements because many chemical processes are dependent on pH. The speed rate of chemical reactions can often be significantly altered by changing the pH of the solution. Apart from that, the solubility of many chemicals in solution and their bio-availability is dependent on pH. The physiological chemistry of living organisms usually has very specific pH boundaries. The term pH derives from a combination of pH for the power and H for the symbol of the element Hydrogen. pH is the negative log of the activity of hydrogen ions. pH = 〖log〗_(10 a) H^+ At a given temperature, pH represents the activity of hydrogen ions in a solution...
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...absorb water from the air and when dried will remain dry for a reasonable period of time. Potassium hydrogen phthalate may be purchased in pure form at reasonable cost. Potassium hydrogen phthalate is a primary standard. This means that carefully prepared solutions of known concentration of potassium hydrogen phthalate may be used to determine, by titration, the concentration of another solution such as sodium hydroxide. The equation for the reaction of potassium hydrogen phthalate with sodium hydroxide is: KCO2C6H4CO2H + NaOH ( KCO2C6H4CO2Na + H2O The equivalence point of a titration occurs when chemically equivalent amounts of acid and base are present. At this point the pH changes rapidly with a small addition of acid or base. If a pH meter is used in the titration and the pH plotted vs the volume of base added, the equivalence point is the middle of the vertical part of the curve....
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...absorb water from the air and when dried will remain dry for a reasonable period of time. Potassium hydrogen phthalate may be purchased in pure form at reasonable cost. Potassium hydrogen phthalate is a primary standard. This means that carefully prepared solutions of known concentration of potassium hydrogen phthalate may be used to determine, by titration, the concentration of another solution such as sodium hydroxide. The equation for the reaction of potassium hydrogen phthalate with sodium hydroxide is: KCO2C6H4CO2H + NaOH ( KCO2C6H4CO2Na + H2O The equivalence point of a titration occurs when chemically equivalent amounts of acid and base are present. At this point the pH changes rapidly with a small addition of acid or base. If a pH meter is used in the titration and the pH plotted vs the volume of base added, the equivalence point is the middle of the vertical part of the curve....
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...Determining the equilibrium constant from pH values of changing concentrations of ethanoic acid Design Research question: How will altering the concentrations of ethanoic acid affect the pH value, and, in-turn, the equilibrium constant? Background information: When weak acids react, the reaction typically does not go to completion. Rather, the system goes to an intermediate state in which the rates of the forward and reverse reactions are equal. Such a system is said to be in chemical equilibrium. When equilibrium is reached, the reactants and the products have concentrations which do not change with time. When in equilibrium at a particular temperature, a reaction mixture obeys the Law of Chemical Equilibrium, which imposes a condition on the concentrations of reactants and products. This condition is expressed in the equilibrium constant Kc for the reaction. In this experiment, I will study the equilibrium properties of the reaction between ethanoic acid, otherwise known as acetic acid (CH3COOH) and water (H2O): CH3COOH(aq) + H2O(l) CH3COO-(aq) + H3O+(aq) When solutions containing ethanoic acid and water are mixed, they react to some extent, forming CH3COO- and H3O+. As a result of the reaction, the equilibrium amounts of CH3COOH and H2O will be less; for every mole of CH3COO- formed, one mole of CH3COOH and one mole of H2O will react. The equilibrium constant expression Kc for Reaction 1 is: Kc = [CH3COOH] / [H3O+][CH3COO-] The value of...
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...INVESTIGATION OXYGEN CONTENT AND PH ON VARIOUS AQUAUTIC ENVIRONMENTS – RIVER IRVINE EXPERIMENTAL BIOLOGY ALISTAIR LAMONT INTRODUCTION THE RIVER IRVINE FLOWS THROUGH THE SOUTH WEST OF SCOTLAND. IT IS AYRSHIRES SECOND LARGEST RIVER. IT HAS NO MAJOR SEWAGE TREATMENT AT ALL, SEWAGE IS PUMPED TO THE COAST TO BE TREATED. IRVINE IS RECOVERING FROM ITS PAST HISTORY AS AN INDUSTRIAL RIVER , ALTHOUGH LEGACY REMAINS AND CAUSES SERIOUS OBSTACLES FOR MIGRATORY FISH. AIM THE AIM OF THIS INVESTIGATION IS TO OBSERVE THE WATER QUALITY IN THE RIVER IRVINE. MATERIALS SAMPLES OF WATER FROM DIFFERENT PARTS OF THE RIVER IRVINE X3 BEAKER X3 PH METER PHOTOMETER CONDUCTIVITY METER METHOD A BEAKER WAS FILLED WITH A SAMPLE FROM THE LOWER PART OF THE RIVER IRVINE, ANOTHER BEAKER FILLED WITH A SAMPLE FROM THE MIDDLE PART AND ALSO THE UPPER SECTION. A PH METER WAS CALIBRATED AND THE PH LEVEL OF THE WATER FROM EACH SAMPLE WAS THEN TESTED AND RECORDER. THE EXACT SAME PROCEDURE THEN DONE USING THE CONDUCTIVITY METER TO TEST THE CONDUCTIVITY OF THE WATER, AGAIN THE RESULT FROM EACH SAMPLE WAS RECORDED. THE PHOTOMETER WAS THEN USED TO TEST THE OXYGEN CONTENT OF THE SAMPLES AND THE RESULTS RECORDED. RESULTS PH LEVEL TABLE 1 REGION OF RIVER IRVINE | PH LEVEL | LOWER | 6.93 | MIDDLE | 7.15 | UPPER | 7.4 | AVERAGE | 7.2 | CONDUCTIVITY LEVEL TABLE 2 REGION OF RIVER IRVINE | CONDUCTIVITY LEVEL | LOWER | 109 | MIDDLE | 78 | UPPER | 22 | AVERAGE | 69.66 | BIO LOGICAL...
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