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Potato Osmolarity

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Change in Potato Cells Due to the Effects of Osmosis (AYE YO POTATO! HOW MUCH OSMOLARITY YOU GOT?)

Introduction: The purpose of this experiment was to determine the osmolarity of a potato. Osmolarity is defined as the solute concentration expressed as molarity. Lerner and Lerner (2008) define osmosis as the movement of water across a membrane that is selectively permeable. Osmosis is important in plant cells because it plays a role in regulating guard cells, which are cells that are on the surface of a plant’s leaves and control the opening of the stoma. When the stoma is open, it increases the water movement out of the plant by a process called transpiration. When there is a shortage of water, the guard cells well get smaller and close the stomata, and the transpiration process will move slower. The osmolarity would be tested with solute concentrations ranging from 0.0 M to 0.6 M. The concentrations increased 0.1 M each time and the solute used was sucrose. The goal of this experiment was to determine which concentration had the least effect on the potato after being incubated. This information helped us estimate the osmolarity of the potato tuber tissue. Our group hypothesis is that the osmolarity of the potato will have the concentration with the 0.1 M solution. This hypothesis is based off the predicted outcome that smaller molarity concentrations will have the least effect on the potato. The more solute added to a solution decreases the concentration of water in most cases (Kosinski). The decrease in water concentration would then lead to a lower weight of the potato tuber once it has finished incubating.

Methods & Materials: For experiment A you will need the following items: 1 large potato tuber Forceps petri dish razor blade DI water metric ruler 7 250 mL beakers/ or disposable cups balance that weighs to the nearest 0.01g sucrose solutions from 0.1M to 0.7M cork borer paper towels calculator

For experiment B you will need the following items: 1 large potato tuber Forceps petri dish razor blade DI water metric ruler 7 250 mL beakers/ or disposable cups Vernier caliper sucrose solutions from 0.1M to 0.7M cork borer paper towels calculator

We took the 7 disposable cups and added 100 mL of the DI water and 100 mL of each sucrose solution in each cup. Then we obtained a large potato to make 7 potato tubers. Using a razor blade, we cut each of the potato tubers to 3 cm long, also making sure we cut the ends of the potato tubers as well. Placing them in a petri dish so they would not dry out, we then weighed each potato tuber on a scale. After recording the weight of the potato tuber, we cut the potato tuber in half long ways. We did this to all the potatoes and then placed each tuber in each cup of solution. We then incubated the potato tubers in the solutions for about 50 minutes. While those potato tubers incubated, we then bored 7 more potato tubers. We then cut the tubers to 3 cm long, doing the same as we did with the first set of tubers making sure both ends of the potato tuber were cut. This time we did not weigh them, but measured each tuber using a Vernier caliper. With the caliper we measured the diameter and the length of each potato tuber. While measuring one potato tuber, we put the others in the petri dish to keep them from drying out. After measuring each potato tuber, we then placed the tubers in the solutions. We waited again about 50 minutes on these tubers to incubate. After the first 50 minutes were up we took the potato tubers cut in half out of the solution. We used a paper towel and blotted the potato tubers. After blotting, we then reweighed both halves of the potato tuber. After re­weighing and recording the results we compared them to the first weights. After the second 50 minutes were up we removed the tubers from the solution, blotting them just as we did the first ones. After blotting we remeasured the diameter and the length of each potato tuber. Recording the new measurements we then compared them to the initial measurements.

Results: Table 1 illustrates the data recorded as the molarity solution experiment was being performed. These are our appropriate results we found to be true. At 0.1M the potato had the smallest weight change of 0.1 g. The potato had the same solute concentration as the 0.1M solution at this time. Graph 1 illustrates the weight change of the potato cylinder while sitting in each sucrose molarity solution when measured with the mass scale. Table 2 illustrates the data recorded as the caliper measurement experiment was being performed. These are our appropriate results we found to be true. At 0.5M, the smallest weight change of the potato was 126.4 mm^3. At this point, the potato had the same solute concentration as the 0.5M solution. Graph 2 represents the change in volume (mm^3) of the potato cylinder when placed in each sucrose molarity solution that were then measured with the caliper. Table 1.

Data for Experiment Estimating Osmolarity by Change in Weight

Approximate time in solutions: 55 minutes (12:55­1:50) Sucrose Molarity 0.0 Final weight (g) Initial Weight (g) Weight Change (g) % Change in Weight 0.1 0.2 0.3 0.4 1.01 1.35 ­.34 0.5 1.03 1.36 ­.33 0.6 .87 1.32 ­.45

1.42 1.35 1.36 1.22 1.35 1.36 1.40 1.41 .07 ­.01 ­.04 ­.19

5.19 .74

2.84 13.48 25.19 24.26 34.09

Graph 1.

Table 2.

Data for Experiment Estimating Osmolarity by Change in Volume Approximate Time in Solutions: 51 minutes (1:23­2:14) Sucrose Molarity 0.0 Final diameter (mm) 7.7 0.1 7.8 0.2 6.7 0.3 6.6 0.4 7.3 0.5 6.7 0.6 6.1

Final length (mm) 42.6 Final volume (mm^3) Initial diameter (mm) Initial length (mm) Initial volume (mm^3) Change in Volume (mm^3) % Change in Volume 1982.7

43.6 2082.3

43.2

39.5

40.6 1698.4

40.5 1427.2

39.6 1156. 7 7.5

1522.3 1350.7

6.5

7.8

7.7

7.8

7.7

6.8

40.3

40.1

40.1

39.6

41.3

42.8

42.3

1337.3

1915.2

1866.4 1891.3

1922.2

1553.6

1867. 8 ­711. 1

645.4

167.1

­344.1

­540.6

­223.8

­126.4

48.3

8.7

18.4

28.6

11.6

8.1

38.1

Graph 2.

Discussion: The experiment estimating osmolarity by change in weight showed the results that the osmolarity of the potato and the molarity of the sucrose solution was 0.1M. The experiment estimating osmolarity by change in volume of the potato using the caliper was 0.5M. One error that could have occurred was only one potato was used to test from, it could have been a bad potato. Another error could have been not swirling the solution with the potato every ten minutes. Inaccurate measurements could have occurred by not blotting the potatoes off completely. The potato cylinder could have bent when measuring, causing the length and or width of the potato to be incorrect. All of the ends may not have been completely cut off of the potatoes. The most important problem was that there was no replication of this experiment. Had we done it again, we could have had a different outcome. Method 1, using the weight to find the osmolarity of the potato was a better fit for finding the real osmolarity of a potato. We found that its osmolarity was 0.1M, which agree with our hypothesis that we thought that the osmolarity of the potato would have the same concentration as the .1M solution. Method 1 could have had its own problems as well, but it showed us exactly what the weight was, although the weight could have been slightly off, it may not have made much difference in our findings. Method 2, using the volume to determine the osmolarity of the potato was not the best fit. Our results did not agree with our hypothesis. There could have been many errors in measuring the potato tubers causing this to not be the best way to test for osmolarity. The biggest weakness of the entire experiment however, is that there was no replication of the experiment. If there is no replication, there is

nothing to compare to see what yields the best results. The significance of the experiment is to test to see what molarity solution the potato plant cells survive the best in, a higher or lower molarity. Scientists can use this information to run tests on certain plants to see how they survive in different environments. With movement of water changing at different rates, some plant cells may die from being in a more hypertonic or hypotonic solution. They need to be maintained at an isotonic solution to ensure the survival of the plant. (Urry et al.) If the plant is placed in a hypertonic solution, this will cause the water inside the cells to be drawn out by osmosis and cause the plants size to decrease. The cytoplasm of the cells will also be affected. The cytoplasm will decrease in size like the plant did, and this means it will shrink away from the cellulose cell wall. All of this will also lead to plasmolysis, which is the lack of structure for the plant causing it to lean or wilt. (Hitchen, Becky.) Conclusion: Our group hypothesis is supported because the osmolarity of the potato was the same as the 0.1M solution that we had predicted. The second method is inaccurate, because the way the potato is measured has a lot of room for errors. No one in our group had ever used a Vernier caliper before, making our measurements most likely inaccurate. With this we conclude that the first method shows our hypothesis to be right. The osmolarity to the potato was changed the least in the solution with 0.1M. This tells us that the potato reached an isotonic state by having a small weight change in a lower molarity solution.

Literature Cited: Hitchen, Becky. "Hypertonic, Isotonic and Hypotonic Solutions and How They Affect Plant and

Animal Cells. ­ Becky Hitchen." Internalandexternalenvironments2012. N.p., 2012. Web. 10 Oct. 2013. Kosinski, Robert. “Osmosis and the Water Potential of Potato Tissue.” Clemson University. N.p.,October 2012. Web. 10 October 2013. "Osmosis (cellular)." The Gale Encyclopedia of Science. Ed. K. Lee Lerner and Brenda Wilmoth Lerner. 4th ed. Detroit: Gale, 2008. Science In Context. Web. 10 Oct. 2013. Urry, L., M. Cain, S. Wasserman, P. Minorsky, R. Jackson, J. Reece. “Membrane Transport and Cell Signaling.” Campbell: Biology in Focus. Pearson Education. Page: 101. 2014.

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