Title:

Abstract:

Introduction:
Background:

PTC or phenylthocarbamide is conveyedby a single geneTAS2R38 that codes for a taste receptor on the tongue was eventually identified in 2003.
The ability to taste PTC is usually associated with dominant inheritance where the inability to taste PTC is to be recessive.
For decades, humans were classified as tasters or nontasters.
The goal of this experiment was to determine 1. Taster Phenotype, 2. Isolate DNA from each individual 3. Determine Taster Genotype

Hypothesis:
Ho: If I am a taster, then my genotype for PTC taster must be either TT (homozygous dominant) or Tt (heterozygous)

The traits of being able to taste versus the inability to taste follows the mendelian inheritance. In mendelian inheritance when two heterozygous dominant parents were mated, genotypic ratios were…..

Methods:
This experiment aimed to investigate the allele frequency of the PTC taster gene (TAS2R38) in a small population, represented by the students in class.
To determine the genotypic profile of the students PTC gene,a sample of DNA was extracted using Chelex resin. Polymerase chain reaction was then used to amplify then digest the gene of TAS2R38 gene with a restriction enzyme. Digested DNA will be cut into 2 fragments 239 bp and 64 bp in length. (Figure 2)
This discrepancy in length allowed us to profile the alleles using gel electrophoresis: non-tasters have only the uncut fragment, taster homozygotes the...The enzyme could either cut the enzyme or leave it whole producing a restriction fragment polymorphism that can be separated by agarose gel