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Renal Lab

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Physiology 12

Benefits of Urine Analysis

Abstract

In this experiment each of our classmates was to give an analysis taken of the sample from their own urine. Each individual was assigned to a group of a specific fluid intake, or to a control group, which had no fluid intake. The fluids included were cranberry juice, water, and Gatorade. The experiment first began with participants emptying their bladders before the lab experiment was to begin, starting at 11.30 a.m. Then each individual drank their assigned fluid, or consumed no fluid if in the control group. Each group was to then repeatedly wait 30 minutes after each urine segment, which consisted of emptying their bladders between the time periods of 11:30 a.m. and 1:30 p.m., noting the quantity of collected urine in ml, color (transparency), s…. and pH balance. Comparing the results, it can be seen that the results of these three variables among the separate groups were similar in reference to the volume in ml, pH, and color. These conclusions can be more readily understood as a result of the statistical graphs which interpolate the given data. From the data we can see that some groups’ urine color turned darker over time, while others’ samples appeared lighter. In addition, the quantity levels increased for some groups, while others remained at the same level. Lastly, the pH levels of the various groups were grouped within a tight range of numerical values.

Introduction

The kidneys are an important organ within the body, which are involved in the filtration of blood and the regulation of extracellular concentrations of water, electrolytes, and certain end products of metabolism. They regulate not only the concentrations of chemicals in the extracellular fluids (ECF) but also the pH and osmotic pressure (Reyes, pp.96)
Our experiment consisted in the examination of urinary sample to test for the presence of particular organic and inorganic constituents, as well as the specific pH levels.
The constituents which may be found within a urine sample include (inorganic): chlorides, phosphates, sulfates, and calcium. In this experiment, tests will be undertaken of the urine sample in search of such substances. This will partially be revealed by the color of the urine. The organic constituents which might be found within the sample include: glucose, ketone bodies, and bilirubin. Typically these substances are found in very limited quantities, and can provide valuable data for consideration from the experimental data.
Since most of the body is water-based (50-60%), the pH level has profound effects on all body chemistry, health and disease. All regulatory mechanisms (including breathing, circulation, digestion, and hormonal production) serve the purpose of balancing pH, by removing caustic metabolized acid residues from body tissues without damaging living cells. “The kidneys maintain normal acid-base balance primarily through the reabsorption of sodium and the tubular secretion of hydrogen and ammonium ions. Urine becomes increasingly acidic as the amount of sodium and excess acid retained by the body increases. Alkaline urine, usually containing bicarbonate-carbonic acid buffer, is normally excreted when there is an excess of base or alkali in the body. Secretion of an acid or alkaline urine by the kidneys is one of the most important mechanisms the body uses to maintain a constant body pH” (www.rnceus.com).
Even the experts, who acknowledge that a neutral or slightly alkaline pH is good, disagree on what this means with regard to body fluids. For example, some researchers consider a slightly acid urine pH to be normal (healthy) because it might indicate that the body is eliminating acids that are a natural product of metabolism. Other experts maintain that the urine should be close to neutral or slightly alkaline. Acid/alkaline balance is extremely important to normal physiology. We will be ascertaining the pH levels of the various urine samples for this experiment.

This experiment was undertaken in order to better understand the functions of the kidney and urinary system. It is our group’s hypothesis that the urine samples as given by our experimental subjects will contain trace amounts of organic constituents, and inorganic constituents which fall within the standard deviations as pronouncing a healthy urinary system. The amount of trace organic constituents will be higher in the urine samples of those who drank cranberry juice (with its correspondingly greater amounts of glucose and fructose), while those who drank Gatorade (with its correspondingly greater amounts of inorganic solutes, including 110 mg of sodium, 30 mg of potassium, and 93 mg of choride (www.gatorade.com)) will show higher levels of inorganic constituents in their urine samples. These increased quantities of constituents should results in a darker urine color. In addition, the greater quantities of constituents results in an increased level of osmolarity, which should result in greater quantities of urine as a result of osmosis. A diet high in meat and cranberry juice will keep the urine acidic (http://www.rnceus.com/ua/uaph.html) Thus, we would expect the cranberry samples to have a lower pH level.

Methods and Materials

Experiment 1:

1. 55 Students 2. Computer 3. Cups w/Lids 4. Latex Gloves 5. pH Strips 6. pH Chart 7. Water 8. Gatorade 9. Cranberry Juice 10. Urine

Subjects were to observe and record the color, volume, and pH of their urine. The experiment took place on Tuesday and Thursday between 2:00p.m. and 9:30p.m. There were a total of 55 subjects who volunteered for this experiment. At 2:00p.m, each subject was to desist from imbibing any food or liquid intake (except for water) until 6:00p.m. Then at 6:45p.m, the subjects were to go to the bathroom and flush out their systems. Each subject was then assigned to one particular drinking group which included: water, cranberry juice, Gatorade, or the control group (no intake). Besides the control group, the other three groups were to drink a cup of their source after the first half hour of class and were to urinate every half hour, between 7:00p.m - 9:00p.m. Each subject was to urinate in a measured cup, observe, and then record data which included pH balance, quantity as measured in mL, transparency, and color. Later, all the data would be analyzed and compared for further results.

Results

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