In experiment 1, we looked at the phagocytosis of fluorescent plastic beads and if macrophages would still eat the beads even though they are not alive. After we looked at the screenshot of all of the cells with the plastic beads, we could confirm that phagocytosis happens to any foreign substance, whether it’s alive or not. We then could move on to the next experiment, 2A. In this experiment, we are looking for the correct amount/concentration of Resolvin D1 that will best enhance the efficiency of efferocytosis. The macrophages that had been treated with the resolvin effector showed the greatest fluorescence, indicating that Resolvin D1 had a positive effect on macrophage activity, as we expected. Our results also indicated that the optimal concentration of Resolvin D1 was 0.25 nM (1:4), as the fluorescence values in our …show more content…
One error was that for Plate 1, we accidentally put the stock solution of Resolvin D1 in columns 6-8, and in column 9 we put the 0.25 nM Resolvin D1 solution. Even though these errors were made, this did not affect the experiment results; it just gave was repeated and unnecessary data. We then could move on to experiment 2B. In this experiment we wanted to determine the effect of certain effectors and also if combining SPMs and cytokines in mixtures and adding them to cells would affect efferocytosis in anyway. For Plate 1, the experiment was largely successful and produced expected results. Resolvin D1 (1:4) + no effector showed to be the best effector for macrophages as the macrophages in this condition took up the largest number of apoptotic cells and then digested the largest amount, resulting with the lowest amount of fluorescence among all conditions at 80 minutes. The macrophages treated with 1:4 Resolvin D1 and IL-13 had the second lowest amount of fluorescence, indicating they had digested the second highest number of apoptotic