...Started with 2 slants of nutrient agar and 2 slants of nutrient broth. 1 agar Petri dish. # 11 We inoculated nutrient agar using the needle by one streak from bottom to top. Broth was inoculated as well. One slant and one broth incubated at 25 C and other slant and broth at 37 C. Purpose 1… To determine optimum temp for bacteria Purpose 2… To start collecting morphological characteristics of colonial growth of the bacteria. Color of bacteria in Petri dish is yellowish beige. There are numerous small colonies. A few colonies are isolated but most seem linked. There is some elevation. Thursday 4/3 Slant 37 C Color of optimum growth slant seems to be a pale yellow color. The pattern of growth is bitted. Smaller rigid or dots are one each side. The bacterial growth seems to be spreading more rapidly on this slant. Soft and easily disturbed. Slant 25 C Color of slant appears a bit lighter yellow. The pattern is bitted as well but not as defined as slant 37 C. It is very similar to the optimum slant except 37 C was more visible and appeared to grow and spread quicker. This slant is tougher and denser than slant 37. This slant feels more jello like as well. Thursday 4/3 Broth 25 C There is a lot of bacterial growth on the bottom of the tube. The broth is pale in color except for the bottom where the growth is visible. The color is a pinkish orange color on the bottom. More Rose like to me. There appear to be round shaped dots as well. Broth 37 C Cluster of bacteria...
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...Analysis of Emily Dickinson’s Poem, “There’s a Certain Slant of Light” In Emily Dickinson’s poem, “There’s a Certain Slant of Light”, the speaker portrays “the slant of light” negatively, suggesting the light is loneliness, sorrow and despair that causes depression (Line 1). I will argue my point by showing how Dickinson uses literary devices to illuminate how depression is the potential demise of the soul. Imagery Dickinson uses imagery sets the stage for loneliness, despair and depression by writing, “There’s a Certain Slant of Light- Winter Afternoons” (Line 1). Here, the speaker asserts that the image of winter’s late afternoon is gloomy, cold, and dark suggesting loneliness. The speaker also asserts that the image of the light is nothing more that depression creeping in, similar to the way late afternoon light does then all of a sudden “it“ is dark like death. The nest 2 lines “That oppresses like the Heft of Cathedral Tunes” further suggests that the light causes a sorrow or heaviness that oppresses the soul like the drudgery of pipe organ music can (Lines3-4). Paradox Dickinson uses a paradox when she writes,”Heavenly hurt it gives us” (Line 5). The use of the world heavenly would make one think initially of peace. When the world hurt is used after it, the likely explanation becomes clearer. The speaker suggests the slant of light which Dickinson throughout the poem refers to as “it”; hurts more than one can fathom. The next three lines back this theory...
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...The process was to inoculate all biochemical tests and plates with bacteria from circled, isolated colonies on the TSA streak plate. Phenyl Red Broth (PR)-Glucose, PR broth-Lactose, Sucrose, Simmons Citrate Slant, Urea Broth, Gelatinase Stab, Triple Sugar Iron Agar (TSI) Slant, Motility, Nitrate broth, Tryptone both, and MR-VP broth test were inoculated and incubated for 24 hours at 37o C. After the 24 hour incubation period, observations were made. The Urea broth was gold and positive. Glucose broth was observed as yellow, positive and acidic. The Simmons Citrate Slant was observed blue and positive. Due to the slant changing to the color of blue it was positive. The sucrose broth observed as yellow and positive. The lactose was observed as pink, which was positive and acidic. Nitrate broth was observed cloudy light yellow or red; the nitrate reduction was performed, which includes incubation of the nitrate broth then add eight drops of reagent A and eight drops of reagent B and mix well. Tryptone (Indole) was observed as light yellow and fermented. MR-VP was not observed. The motility was observed as cloudy and moving, which made it positive. Gelatin was observed remained gelatin, which made it negative and no change. On the TSI slant was observed as Red/yellow the colors were separated, which made this positive...
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...Trypticase soy agar streak-plate prepared with: *Micrococcus roseus and Micrococcus luteus (1) Trypticase soy agar streak-plate prepared with: *Escherichia coli and Micrococcus luteus (4) Trypticase soy agar slants Bunsen burner Inoculating loop Permanent Marker Test tube rack Straight needle Striker Incubator Method Part A: Isolation of Discrete Colonies from a Mixed Culture * Label one Trypticase soy agar streak-plate using a permanent marker on the b ottom. Write the date (1/23/14), TTH2, student’s initials, the names M. roseus and M. luteus. There were also lines dividing the streak-plate into four sections with one section labeled I, one labeled II, one labeled III, and one labeled IV on the bottom of the plate. The quadrants should look like the labeled circle below. * Divide a second Trypticase soy agar streak-plate into four sections and label with the Roman numerals in the same way as the first plate. Also label the bottom of this plate with the student’s initials, TTH2, and the date (1/23/14). Write E. coli and M. luteus on the bottom of this plate. * Turn on the gas and light the Bunsen burner using a striker. * Bend the inoculating loop to make...
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...Dickinson states “ the eyes around - had wrung them dry-” which means the others had cried to a point they could no longer shed tears. Next the poem goes on about people gathering for the last day the person would be seen in a room. This may be referring to a hospital bed or funeral. In the third stanza “I willed my Keepsakes - Signed away” suggests the person is writing out their will. The unknown person is deciding what to put in their will making their life seem like it could be signed away. During this time a fly interrupts them. In the last stanza the person is dying and the last thing they heard was the fly’s buzz. The person was the stuck between the fly’s buzz and the light to the afterlight.Until finally the last line “ I could not see to see-” this is the very last moment in this person's life as the light faded away. The poem is about a person whose last memory before death took a toll is a fly...
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...broadcasted interview. When I listened to Giuliani and King talk about 9/11, I didn’t feel as though there was any kind of slant. As Giuliani and King reflect back on 9/11, it shows that not only are they still affected by the tragedy, but so is America. I believe Giuliani to be credible because he experienced 9/11 himself. I am not skeptical on his credibility because during 9/11, Giuliani was in the middle of the chaos trying to help others. He holds those memories dear to his heart, and will continue to for many years to come. I did not detect any bias from him because he was speaking from his personal opinion, and experience. The commercial from the Pedigree website was also paid by Pedigree and its creators. In my opinion, there was no slant in the Pedigree commercial. The Pedigree commercial lets me know that Pedigree is just another type of dog food trying to persuade animal owners into buying their brand because they say it is healthy. I do not find Pedigree to be credible because there is no proof that they are healthier than other brands. In the Pedigree ad, they show a dog and a person in the car and the car tilts over claiming the dog is overweight. They are also claiming that their dog food is healthy for overweight dogs. After seeing this ad, consumers may have the desire to switch their dog food over to Pedigree because they have a new “light” version for dogs that need to be on a diet. I think this ad tries to create a new fear for consumers. Some dog owners might...
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...Progressive Commercial: Meant to be Together * In the commercials from Progressive, the ads were paid for by Progressive. Progressive’s slant to their commercial is that Progressive insurance makes everything better. If it is the outdoors then Progressive makes being outside more fun and a better time overall. The examples given by Progressive show that if it is fun it is Progressive, this commercial does not concentrate on prices they just concentrate on showing people having fun, I believe that it reflects the believe that if people are having it appeals to more people, especially at this time with the economic problems that everyone is having. Wendy’s Commercial: Wild Berry Frosting * In the commercials from Wendy’s the ads were paid for by Wendy’s. Wendy’s slant to their commercial is that by having a Wendy’s product you can experience a mini vacation. The commercials from Wendy’s once again have the same reflection of society that Progressive’s commercials have, that they make everything more fun. I think that at this moment in time with the economic problems and everything that is happening, people are more apt to go for something that appeals to the light hearted, to their happier moments. 2nd Part I am not skeptical of Giuliani’s credibility as he, as well as every American, knows what happened on 9/11. His sincerity came across clearly and he was in no position to fake the topic, especially since it creates a very strong emotional feedback from almost...
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...Aseptic Technique and Culturing Microbes Aseptic Technique and Culturing Microbes September 30th Abstract: The purpose of this lab is to learn and gain knowledge about aseptic techniques to collect and transfer cultures, learning about different culture medias and proper temperature to grow bacteria. Materials used: -Incubator made from box, styrofoam pieces wrapped with foil -Thermometer -S.epidermise sample -L.acidophillus -Lamp -Broth MRS-9ml -Broth,Nutrient -Mask -Gloves -10% bleach -Sterile swab stick -Paper towel A. There is a difference between an Obligate and Faculative Aerobe. • An Obligative aerobe is an organism that requires oxygen to grow. They can only survives in the presence of oxygen. • Faculative aerobe is an organism that can grow in the presence and as well as in the abscense of oxygen but it grows more faster in the abscense of oxygen. B. To grow L.acidophillus and S.epidermice bacteria it needs approximate 37.o degree C temperature. C. To control or to prevent the growth of microorganisms it has two different ways .It is by killing microorganisms or by inhibiting the growth of microorganisms. Physical or chemical agents are used for it. Agents that kills bacterias are called bactericidal and the agent that slows the growth of bacterias are called bacteriostatics. There are different methods of killing and controlling the growth of microorganisms like Incineration, Sterilization, Irriadiation, Filtration, Toxic...
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...A ∑ This eBook is provided by www.PlentyofeBooks.net E= mc 2 Plenty of eBooks is a blog with an aim of helping people, especially students, who cannot afford to buy some costly books from the market. For more Free eBooks and educational material visit www.PlentyofeBooks.net Uploaded By Bhavesh Pamecha (samsexy98) 1 Handwriting Analysis The CompZete Basic Book NEW PAGE BOOKS A division of The Career Press, Inc. Franklin Lakes, NJ Copyright 0 1980 by Karen Amend and Mary S . Ruiz All rights reserved under the Pan-American and International Copyright Conventions. This book may not be reproduced, in whole or in part, in any form or by any means electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system now known or hereafter invented, without written permission from the publisher, The Career Press. HANDWRITING ANALYSIS ISBN 0-87877-050-X Printed in the U.S.A. by Book-mart Press To order this title, please call toll-fiee I-800-CAREER-1 (NJ and Canada: 201-848-0310) to order using VISA or MasterCard, or for fbrther information on books fiom Career Press. The Career Press, Inc., 3 Tice Road, PO Box 687, Franklin Lakes, NJ 07417 The author of this book does not dispense medical advice nor prescribe the use of any technique as a form of treatment for medical problems without the advice of a physician, either directly or indirectly. The intent of the author is only to offer information.of a general...
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...WAAS Update This year AEC created an amendment to the WAAS STC for the EC-145. The existing commercially developed EC-145 STC for WAAS implementation modified an aircraft with dual Garmin GNS-430 VOR/LOC/COM/GPS units, a DM 451 Distance Measuring Equipment (DME) system, and dual FCDM/SMD-45 systems. The final configuration for this aircraft allows Single Pilot/Dual Pilot Instrument Flight Rules (SP/DPIFR) with NMS1-2 switching capable of GPS display on either FCDM/SMD-45 system. The specific aircraft that was used for the initial STC work had several items of equipment that were installed outside of the Type Certificated option choices. As a result, the aircraft pre-modification configuration for the prototype installation of the STC was very different from the Type Certificated options pre-modification configuration. The revision of the STC to account for the configuration differences is required in order to allow for a broader aircraft serial number applicability baseline. A large number of MBB-BK 117C-2 aircraft serial numbers have no provisions for display of the #1 GPS on the FCDM/SMD-45 systems since the NMS1-2 switching option was not installed in these aircraft. The baseline MBB-BK 117C-2 aircraft GPS system status annunciator location are not within the primary field of view of the flight crew as defined in FAA guidance material. This has important implications to be detailed later in this document. There are further issues with the certification basis and physical...
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...Clinical Microbiology Lab Final December 13, 2013 Table of content Gram Stain Technique……………………………………………………………………………………………… page 1 Culture Transfer Technique……………………………………………………………………………………… page 2 Acid-Fast Stain Technique………………………………………………………………………………………… page 3 The importance of the Gram Stain Technique to a physician……………………………………. page 4 The importance of varying shapes/colonies formation of bacteria……………………………. page 5 Spore Stain Technique………………………………………………………………………………………………. page 6 The Importance of incubation/protocol techniques…………………………………………………... page 7 The importance of various types of media for bacterial growth…………………………………. page 7 The importance of biochemical analysis in the microbial process……………………………… page 8 The importance of studying Clinical Microbiology and how the course will assist me in reaching my professional goals……………………….. page 9 Bibliography……………………………………………………………………………………………………………… page 10 Gram Stain Technique The Gram Stain is one of the most important differential stains used in bacteriology. (Cappuccino and Sherman, Microbiology A Laboratory Manual) Using the gram stain it is possible to determine purple gram-positive cells (S. aureus) from pink gram-negative cells (E. coli). The results of the Gram Stain make it possible to identify microorganisms by their shape, number and morphology. In a clinical setting these results can help in treatment by identifying the type of microorganism...
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...HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY GENERAL Unknown reports in microbiology are written in scientific format. Scientific writing is written differently from other types of writing. The results of the exercise or experiment are what are being showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. The writing should be simple and easy to understand. There is a specific style that must be followed when writing scientific reports. Scientific writing is typically written in the passive voice. The pronouns "I", "We" and "They" are not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the unknown." It is also customary to write in the past tense for most of the report. This includes the introduction, the summary, the description of the materials and methods and the results. The present tense is reserved for the conclusions about the results. See the examples given below. Some other general rules that should be followed are: Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name should be italicized or underlined. Italicized is preferred. For example, Staphylococcus aureus. The genus is capitalized but the species is not. After the full genus name is given in the paper, it can be written as S. aureus, but still italicized. This is as long as there in no other...
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...Week Seven Lab Discussion Each team member completed an oxidation-reduction lab during week six. The team members then shared their findings with each other for comparison and discussion. The purpose of this lab was to determine relative oxidizing and reducing strengths of a series of metals and corresponding ions. Oxidation and reduction reactions occur simultaneously. In oxidation, electrons are given, and in reduction, electrons are gained. An oxidizing agent is a chemical substance, which has a large tendency to gain electrons, while a reducing agent is an element, which loses electrons to another chemical species and is therefore oxidized. This lab used metals and provided substances to determine reactions. These results were recorded in a data table and the results were analyzed based on these observations. These results were used to help the team gain a better understanding of the reactions between the metals and the solutions. Discuss and Compare End-of-Lab Questions Data Table Results: Magnesium (Mg) in sodium sulfate (Na2SO4): three team members observed bubbling, two stated that they observed no reaction, and one observed only a slight reaction. Zinc (Zn) in magnesium sulfate (MgSO4): no team members observed a reaction. Lead (Pb) in zinc nitrate (Zn(NO3)2: five members did not observe a reaction, while one member noted and removed a layer of tarnish, and last member stated that it formed a clear coating on the lead. Lead (Pb) in iron (III) chloride...
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...Sylvia Plath (1932-1963) An American poet and novelist of the 20th century was born in Boston, Massachusetts. Sylvia Plath is best known for her semi-autobiographical novel The Bell Jar and her second volume of poetry collection, Ariel. She was one of the most dynamic and expressive poets of her time. As a student she was successful, won many awards and scholarships ,at the age of 11 she started keeping a journal of her poems of which many were published in her early years itself. However, inspite of such perfections in her academic life she felt anything but perfect in her own skin. Her poems show her deep anguishes with her own life involving her broken marriage with Ted Hughes, unresolved issues with her parents with so much light on the passing of her father when she was only eight and her own vision of herself. At the time of her undergraduate years she has started showing symptoms of severe depression and already had a history of mental illness since childhood which ultimately lead her to her death. Her conditions led her to try to commit suicide not once but twice before she finally succeeded the third time. She had a sort of disturbed mind which can be felt through her much personal poems such as “daddy” which brings out her deep insecurities of being “fatherless” Feminists potrayed Sylvia as a woman driven to madness by a domineering father, unfaithful husband and demanding duties of motherhood. The hardness of her life increased her need to write ,which she could...
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...Process and Techniques of Finding Unknown J Abstract: As part of a focal study within Microbiology, many scientists have endeavored to identify the many species and types of microbes found in different environments. In an effort to categorize, understand and distinguish one microbe from another, scientists developed tests to show unique characteristics of microbes. This experiment enlists these tests, such as PCR, Simple and Gram staining, anaerobic growth tests, IMViC, Catalase, Oxidase, selective and differential media to identify an unknown microorganism. The Unknown organism studied was labeled “J” and found to be a gram negative, rod shaped bacteria that does not produce endospores. The selective and differential agars produced no growth on the MSA agar plate showing that the bacteria did not favor a salty environment of the Mannitol salts and showed an acidic by product in the selective and differential media of MacConkey’s Agar. The bacteria showed to metabolize sugars but did not produce any gaseous byproducts. After 16s rRNA was processed and run through a PCR, electricphoresis was used to run the RNA out on a gel in order to sequence the RNA which was then compared in a database. Furthermore, the Unknown J did not produce or metabolize starch. The bacteria did not react with the Citrate, KEY oxidase. When compared to other known bacteria tested, unknown J proved to be Escherichia coli. Introduction: Identifying bacterial causes for certain diseases that have...
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