...I. 진단혈액 진단혈액 수련항목 (1) : 혈액학적검사 기본 술기 표준수련기간 : 1주 수련내용 : ◆ 용어정의 : • 혈액학적검사 : 혈액세포와 응고에 관련된 일련의 검사를 의미한다. 혈구의 체내분포, 구조, 기능에 관련된 검사, 골수에 분포하는 전구세포에 관한 검사, 혈구에 영향을 끼칠 수 있는 혈장 인자에 관한 검사 및 유전자 이상에 관련된 검사 등을 포괄적으로 포함한다. • 망상적혈구수 : 적혈구 성숙 단계 중 정염색성 적아구(orthochromatophilic normoblast) 바로 다음 단계의 세포로 핵이 빠져나간 직후부터를 의미한다. 미토콘드리아, 중심소체(centriole), 리보솜 등을 함유하고 있으며 말초혈액에서 24-48시간의 성숙과정을 거쳐서 성숙한 적혈구로 된다 (Ref. Williams 16th p373-374) ◆ 숙지할 필수 지식 : • 혈액학 검사에 사용되는 검체와 항응고제의 작용기전 및 종류 • 모세관 혈액의 채취 방법과 용도, 채취 시 주의점 및 정맥혈과의 차이점 • 적혈구침강계수(ESR) 검사의 원리 ◆ 습득할 필수 술기 : • Neubauer chamber의 사용 • 미량법(micromethod)를 이용한 헤마토크리트의 측정 • 수기법을 이용한 망상적혈구수 검사 ◆ 국내외 장비 및 시약 현황 : 해당없음 ◆ 추천되는 참고자료 : • 대한혈액학회. 혈액학, 2006. • 대한진단검사의학회 편, 진단검사의학 제 3판, 2001. • Henry, JB. Clinical Diagnosis and Management by Laboratory Methods, 24th ed. 2006. 보고서 제출 일자 : 200 년 월 일 평가자 : 지도전문의 인 (일자 : 200 년 월 일) 과장 인 (일자 : 200 년 월 일) 수련위원 인 (일자 : 200 년 월 일) 진단혈액 수련항목 (2) : 자동 혈구계산기 표준수련기간 : 2주 수련내용 : ◆ 용어정의 : • 헤마토크릿(Hct) : 혈액 전체 부피에 대한 적혈구 부피의 비율, 단위는 % 또는 L/L • 평균적혈구용적(MCV) : 적혈구의 평균 용적, 단위는 fL, • MCV = Hct (L/L) X 1,000/RBC count (X1012/L) • 평균적혈구혈색소(MCH) : 적혈구 한 개당 혈색소 양, 단위는 pg, • MCH = hemoglobin (g/L)/RBC count (X1012/L) • 평균적혈구혈색소농도(MCHC) : 적혈구 한 개당 평균 혈색소 농도, • 단위는 g/L, MCHC = hemoglobin (g/L)/Hct (L/L) • 적혈구분포지수(RDW)...
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...Introduction Every chemical compound absorbs, transmit or reflect light over a certain range of wavelength. In this experiment one of the main purpose is to introduce students to the quantitative and qualitative study of spectrophotometry. Spectrometry is the measurement of light that is absorbed or transmitted by the sample solution (Vo, n.d). It is one of the most commonly used methods in various fields to study the quantitative analysis of light that passes through a solution. Meanwhile, the use of spectrophotometer to determine the extent of absorption of wavelengths of visible of light is known as colorimetry. The intensity of light that passes through the sample as well as the concentration of the substance is measured by a spectrophotometer. The instrument consists of some basic components: Radiation light source (tungsten for visible region and deuterium lamp for ultraviolet region), wavelength selector (to isolate a desired wavelength form the source), a cuvette or container to hold the sample solution and a detector (photometer) which delivers the signal in a display device. The voltage output is determined by the difference in the amount of light absorbed by the sample. The fraction of light that passes through the solution is called transmittance, T and the proportion of light absorbed is the absorbance, Abs. Thus, the value of transmittance can be calculated by using the following formula: Transmittance, I = It/ I0 where, It = intensity of...
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...4A. Vesicular transfersome and liposome based transdermal gel of aceclofenac: 4A.1 Preformulation studies: Identification of drug was carried out by DSC, FTIR and UV spectrophotometry 4A.1.1 DSC studies-DSC studies were carried out at a temperature range of 25 º C to 165 º C at 10 º C increments of temperature. Figure4A.1-DSC Thermogram of Aceclofenac DSC thermogram indicates the M.P of 154º C which fairly matches with reported M.P of Aceclofenac, thus confirming the identity and purity of aceclofenac. 4A.1.2. FTIR studies- Figure4A.2 . FTIR spectra of Aceclofenac Figure.4A.3 Standard FTIR spectra of aceclofenac FTIR Interpretation: Figure 4A.4 Chemical structure of aceclofenac Table 4A.1 Interpretation...
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...Dr. Hjorth-Gustin Chemistry 201 Lab November 8th, 2010 Synthesis and Analysis of Iron(III) Oxalate Complex Discussion This experiment initially involved the synthesis of an iron (III) oxalate complex with the general formula Kw[Fex(C2O4)y] zH2O. The variables x, y, and z were determined through the duration of the entire experiment. Part 1 involves the synthesis of an iron (III) oxalate complex. The iron is first presented in its Fe2+ form, so it must first be oxidized to Fe3+ before the oxalate ion will readily bind to it. Hydrogen peroxide is the oxidant of choice: 2Fe2+ (aq) + H2O2 (l) + 2H+ (aq) ---> 2Fe3+ (aq) + 2H2O (l), in acidic solution. The oxalate ion is then free to coordinate to the Fe3+ ion, forming a complex of Fe(C2O4). The oxalate ion is the conjugate base of the weak oxalic acid, H2C2O4. In the synthesis of the iron (III) oxalate complex, 0.8668g of the final lime-green crystals were obtained. The average percent of C2O4 was 59.00% and the theoretical yield was 53.74%. With this, the percent error came out to 9.79%. The percentage of Fe(III) in the green crystal obtained was 14.5%. Theoretically, it should have been around 10%. This lack of accuracy was quite difficult to recognize considering the calculations were approved by the professor but may have been due to incorrectly preparing the buffer. Apart from the usual human-mediated errors in the measuring and distribution of chemicals and in the readings of instrumental...
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...S W I S S G E R M A N U N I V E R S I T Y INORGANIC & ORGANIC CHEMISTRY LABORATORY REPORT | Subject | : Inorganic & Organic Chemistry Laboratory | Lecturer | : Hery Sutanto S.Si | Instructor | : Tabligh Permana S.Si., Dian Sukmayanda S.Si | Faculty/Class | : Life Science/LS 2 A | Date of Experiment | : 11 April 2012 | Date of Lab. Report | : 18 April 2012 | Semester | : 2 | Time of Experiment | : 14.00-17.00 | | | | | | | | | | | | | | | | Experiment: | Principle of Spectroscopy | NAME : Melisa Grace (14211043) Nur Ratih K. (14111005) Group : G | | Campus BSD CityBumi Serpong DamaiTangerang 15321 – Indonesia | Tel. +62 21 537 6221 Fax. +62 21 537 6201 sgu.info@sgu.ac.id www.sgu.ac.id | EXPERIMENT 5: Extraction of Caffeine From Tea Leaves 1. Objective: To demonstrate the extraction of Caffeine as natural substance by using organic solvent and distillation technique. 2. The Materials, Equipments and Procedures: A) Materials * K2CrO4(Potassium Chromate) * H2SO4 (Sulphuric Acid) * Aquades B) Equipments * Beaker * Volumetric flask (50 ml and 25 ml) * Glass rod * Spatula * Watch glass * Graduated pipette * Pipette * Scale * UV-Vis spectrophotometer * Cuvette C) Methods 1. Equipment and materials necessary for the experiment were prepared on the working table. 2. Calculation...
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...BIOL2103 Biological Sciences Laboratory Course Practical 3 Laboratory manual Isolation of nucleic acid and spectrophotometry Introduction: The ability to isolate and quantify nucleic acids accurately and rapidly is a prerequisite for many of the methods used in biochemistry and molecular biology. The concentration of DNA or RNA in a sample, and its condition, are often estimated by running the sample on an agarose gel. Such concentration estimates are semiquantitative at best and are time-consuming. For a more accurate determination of the concentration of DNA or RNA in a sample, a UV spectrophotometer is commonly used. Spectrophotometry uses the fact that there is a relationship between the absorption of ultraviolet light by DNA/RNA and its concentration in a sample. The absorption maximum of DNA/RNA is approx 260nm. The purity of a solution of DNA can be determined using a comparison of the optical density values of the solution at various wavelengths. For pure DNA, the observed A260/A280 ratio will be near 1.8. Elevated ratios usually indicate the presence of RNA. The A260/A280 ratio is used to assess RNA purity. An A260/A280 ratio of 1.8-2.1 is indicative of highly purified RNA. The 260/280 ratio below 1.8 often signal the presence of a contaminating protein or phenol. Alternatively, protein or phenol contamination is indicated by 230/260 ratios greater than 0.5. Workflow Time 2 days before the lab session During lab session 1:30 pm Task Cell...
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...a polysaccharide. Taking advantage of the bimolecular interactions that sugars have with particular enzymes, these substances can be oxidized and converted into other useful byproducts. In this experiment, the concentration of glucose will be determined in solutions containing diluted Gatorade and enzyme mix, among other contributing reagents. The role of the enzyme mix is to provide a way to accurately measure the glucose. The enzymes used, glucose oxidase and horseradish peroxidase, are ideal for this experiment due to their selective nature of binding to particular molecules that fit them. The byproducts produced from the enzymatic reactions provide a component (ferricyanide), who’s absorbance is then measurable through spectrophotometry. Spectrophotometry involves the passing of light through a substance to measure the resulted intensity that passed completely through. Changes in temperature, reaction time, and volume of enzyme mix will also be examined to observe the effect of each on the resulting glucose...
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...IDENTIFICATION OF STATINS IN RICE FERMENTED WITH MONASCUS SPECIES FROM THE MUSEUM OF NATURAL HISTORY Jude Carlo J. Muca1, Reigna S. Romero1, Ricardo R. Santos², Roberto Z. Yuseco3, Florence M. Blanco1, Lei Anne C. Carolino1, Engkhuan Chew1, Ericka Joy B. De Guzman1, Jordan Carlo S. Galang1, Jin-Gu Lee1, Lawrence Y. Maliwat1, Marixie Ann Q. Manarang1, Jeshua Caleb B. Miole1, Michael Henry B. Piano1, Lou Anthony S. Sico1, Mark Lester I. Tolenada1, Mellanie B. Victoria1 1 Medical Student, Angeles University Foundation, Angeles City 2009; ²Head, Department of Biochemistry, School of Medicine, Angeles University Foundation, Angeles City 2009; 3 Faculty, Department of Biochemistry, School of Medicine, Angeles University Foundation, Angeles City 2009; ABSTRACT Pharmaceutical intervention through statin drugs is the most common way of slowing down the adverse effects of heart disease due to cholesterol deposition by regulating the activity of HMG-CoA reductase which catalyzes conversion of HMG-CoA to mevalonate. In order to seek alternatives for commercially available statin drugs, this study idnetified the statins that can be produced by fermentation of rice by Monascus strains available at the Museum of Natural History (MNH). It also shall serve as a set-off point for further studies regarding the use of fermentation products for treatment of certain ailments, such as heart disease. A total of eight (8) isolates available at the Museum of Natural History (MNH) of the University...
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...+ 2H2O(l) → Cu(H2O)42+(aq) + 2NO2(g) + 2NO3-(aq) Zn(s) + 4HNO3(aq) + 2H2O(l) → Zn(H2O)42+(aq) + 2NO2(g) + 2NO3-(aq) These reactions produce the complex ions of Cu and Zn: Cu(H2O)42+ and Zn(H2O)42+. Complex ions are made of a central atom covalently bonded to two or more anions or molecules called ligands. Ammonia can be replace the water ligands by the reactions: Cu(H2O)42+(aq) + 4NH3(aq) → Cu(NH3)42+(aq) + 4H2O(l) Zn(H2O)42+(aq) + 4NH3(aq) → Zn(NH3)42+(aq) + 4H2O(l) Cu(NH3)42+ is deep blue and Zn(NH3)42+ is colorless, making spectrophotometry a possible option to measure the concentration of copper in the solution. The absorbance of the solution can be evaluated with a spectrophotometer, and according to the Beer-Lambert Law, the amount of light energy absorbed at a particular wavelength by a compound in solution is proportional to its concentration in that solution: A = abc The objectives of this lab include learning the fundamentals of spectrophotometry and the Beer-Lambert Law, learning how to use a spectrophotometer, preparing and using a calibration curve based on the Beer-Lambert Law, and learning about oxidation of less active metals and complex ion formation. The purpose of this lab is to experimentally determine the amount of copper in a penny using the absorbance characteristics of the complex ion Cu(NH3)42+. The hypothesis of this experiment is Methods: Solution Preparation: To create the standard solutions, 0.00 mL, 2.00 mL, 4.00 mL, 6.00 mL, 8.00 mL and 10.00 mL...
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...At Coast to Coast Flavors, we manufacture and blend flavor concentrates and syrups for the beverage and food service industries. Coast to Coast Flavors’ customers include Fortune 500 and other large corporations which require strict quality control and manufacturing standards. We are committed to consistently providing products of the highest quality to all of our customers as well as providing a level of customer service unmatched in our industry. Our on-site research laboratory supports a state of the art quality assurance platform. The lab is fully furnished and staffed to complete gas chromatography, spectrophotometry, and microbiological testing in support of both the quality assurance program and our product expansion activities. Our quality assurance program characteristically includes production-batch testing and sample preservation and can be customized to the special needs of our customers. The company's laboratory employs full-time staff members to perform quality assurance and product development activities. Ready-2-Use Syrups create a variety of mouthwatering snowball treats for your family, friends or customers. Available in 18 mouthwatering flavors, C2C syrups are easy to use ... simply pour over shaved ice. If more variety is desired, try our IcyOrb brand of concentrates. With 52 available flavors, one for each week of the year, the possibilities are boundless for producing thought-provoking flavor and color combinations. We like to please as many customers...
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...high energy chemical molecules that become subunits of carbohydrates. There are four different pigment groups present in leaves of photosynthesizing plants. Studies indicate that only the chlorophyll IS involved in the actual absorption of light energy and later conversion to chemical energy of living cells. The other pigments also absorb light energy, but it is transferred to the chlorophyll for conversion to chemical energy. Biochemists have developed a variety of methods for the purification and analysis of biomolecules. Several of these techniques will be used in this laboratory exercise in order to isolate and study the photosynthetic pigments, chlorophyll a, chlorophyll b, and carotenoids. These include paper chromatography and spectrophotometry. Paper chromatography separates compounds on paper as solvent carries the mixture...
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...| Shailesh Thapa | | | 949 Dorchester Ave Apt 2, Dorchester, MA 02125 6177043638 candidboy18@yahoo.com | ObjectivesTo gain experience in the biotechnology company as an intern and learn more about the opportunities in the biotechnical field.Education * University of Massachusetts Boston(2014)Bunker Hill Community College (2014) * Related Course Work: Cell Biology (lab) * Dickinson State University (2010-2013) * Related Course Work: Genetics (Lab) , Microbiology (Lab), Organic Chemistry I and II(Lab) experience Student Senate Head Delegate | Dickinson State Student SenateMay 2012 – May 2013 * -Represent Dickinson State University (DSU) in North Dakota Student Association meetings and inform about current activities of students in DSU * -Arrange the hotels, vehicles and amount of money for students travelling for the NDSA meetings. * -Help organize any meetings that are going to take place in the University * * President of DSU International Club * Dec 2012-May 2013 * -Organize different International New years and lead the international club. * -Help students with any kind of difficulties and present it to the student senate. * * DSU Student Center, Front desk * June 2012- Jan 2013 * -Help students with all kinds of campus related activities, and provide the right information * -Answer phone calls from all over the world and guide them to the right department * -Guide guests and guardians to the right office * -Organize and arrange...
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...was first opened in 1793, and pennies were originally crafted out of 100% pure copper. By 1857, the composition of the penny was altered to incorporate nickel and then switched to a combination of tin and zinc in 1864. Due to a need for copper in World War II, pennies later became zinc-coated steel in 1943. The final alteration to the penny’s composition was made in 1983 when the tin was eliminated, and pennies became primarily zinc; only containing about 2.5% copper. Meredith, 2022. For this experiment, only pennies minted from 1983 and newer were used, so that the exact amount of copper in a penny could be found and all used pennies would hold about the same amount. This experiment also used spectrophotometry, to help determine the percent of copper in the pennies. Spectrophotometry is a method used to measure how much light a chemical substance absorbs by “measuring the intensity of light as a beam of light passes through a sample solution” (Vo, 2013). There are two components that make up a spectrophotometer, one being the spectrometer that produces different wavelengths of light, which in turn is used to transmit photons. The other component is the photometer, which detects the number of photons that are absorbed by a solution (Vo, 2013). For this experiment, the spectrophotometer was used to measure the absorption of known samples which would later be used to find the concentration and absorption of the copper penny solution. The information gathered by the spectrophotometer...
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...To begin, MOs were suspended and hydrated in high-grade water, so that they could maintain concentration. Additionally, the solution in which the MOs existed was isotonic, and centrifuged to remove any unwanted particles. The concentration of the MOs was then assessed through spectrophotometry, to allow for proper dosage for injections into the zebrafish embryos. Before injection of the MOs into the embryos, the needle was calibrated with 10 thirty second pulses, and then attached to a foot pedal and placed under a dissecting microscope to allow for equal amounts of injection in each embryo. MOs are injected into the yolk of the embryos to reduce the chances of disruption due to secondary effects in the blastomere stage. The researches injected the embryos between the 1 and 8 cell stage with a dosage of 1.5-6 ng. In this experiment two types of injections were tested, one in which the MOs phenocopied existing mutations already present in the zebrafish, and the other in which MOs were added until a threshold of the reduced...
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...Effects of Temperature on Membrane Permeability on Beta vulgaris Abstract In this experiment, we studied the effect of temperature on the cell membrane of a common garden beet Beta vulgaris. Beet roots were washed thoroughly, cut into six cylinders and then placed in separate test tubes. We placed each test tube in separate water baths at different temperatures for a specific period of time. The test tubes were then removed from the water baths and the absorbance of the solutions containing betalain pigments was recorded using a spectrophotometer . Maximum absorbance was recorded at -8°C (followed by the absorbance at 78.9 °C) indicating maximum release of pigments from the cell while minimum absorbance was recorded at 8°C. The results of the experiment were mostly consistent with the expectations and it was found that with increase in temperature the absorbance increases and so does the release of pigments from the cell. Introduction Cell is the basic structural and functional unit of life . The word cell comes from the Latin word ‘cella’ , meaning small room. Cell was first discovered by Robert Hooke as a descriptive term for the smallest living biological creature. The cell theory, developed by Schleiden and Schwann states that all cells arise from pre- existing cells by cell division , each cell acts as an elementary organism and all organisms are composed of one or more cells. On the basis of number of cells organisms can be classified as unicellular...
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