...power of sorghum and rapoko malts * to be able to compare the diastatic power between rapoko malt and sorghum malt SUMMARY Sorghum and rapoko malts were produced by grinding using pestle and mortar until 25g of the malt was obtained for each sample, transferred to 500ml flask and 0.5% NaCl was added , the mixture was closed and swirled. Time was noted and the infusion was left to stand for 2,5 hours at 20°C agitating it by rotation at 20 minutes intervals. Infusion was filtered thereafter through 32cm fluted filter and first 50ml was returned to filtrate and the final 20ml was transferred to a 100ml volumetric flask and was diluted to the mark with 0,5% NaCl .10ml of solution was pippeted to a 250ml flask and brought to 20°C. 200ml of starch solution was added to the flask and maintained at 20°C for 30 minutes. 250ml of 0.5NNaOH was added and mixed by inverting the flask, and topped to the mark with distilled water. A blank solution was prepared . 10ml of Soxhlet solution and 10ml of water were added to a 200ml volumetric flask and boiled. Titration was done into simmering solution in flask, on cooling blue colour returns. Rapoko had a volume of 230mls , sorghum 270mls. Blank of rapoko had 222ml and that of sorghum had 270ml. The diastatic power of rapoko was 21°and sorghum was 18°. INTRODUCTION The diastatic power (DP), also called the diastatic activity" or "enzymatic power", of a grain in general refers only...
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...Corn has been a large part of many cultures. In Mayan Corn was such an important crop to them that they actually gave sacrifices to ensure that the Corn crops came out with abundance. With all of the changing it has done many do not understand that Corn is actually a grass and a sophisticated one at that. Corn has adapted itself to many environments and has changed forms dramatically over decades of being grown. Although Corn has advanced many times over, it still requires human intervention. Corn has been so much more than just food for people. From creating food for the livestock we grow and eat to being used in many everyday products from medicines down to the rubber in tires and gas in cars. While for many years Corn was thought to be just another vegetable Corn is actually grass that has evolved many different ways, been implemented in many products, and has become a part of everyday life. Corn has many points where evolution has taken its role in the Corn we know today (Omnivores Dilemma P. 37-38). The structure of Corn has been a crucial factor for its survival. The structure of Corn cannot be weak and flimsy; it has to withstand harsh weather, plowing, birds, and or insects. Many times Corn can adjust itself to the different climates whether there is abundance of water or lack thereof. “Like a giant wick, a growing corn plant pulls moisture out of the soil. Some of that moisture escapes through the plant’s leaves and enters the atmosphere (transpiration). Water vapor...
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...Corn starch production process: Clean up except miscellaneous - soaked - crushed - germ separation - grinding - starch sizing - protein separation, starch sieve washing - centrifugal separation, drying. Corn starch processing Major equipment: 1. Elevator 2. Clean the screen 3. stone remove channel 4. Sulfurous acid tank 5. Sulfur absorption tower 6. Soak pot 7. 8. Gravity crushing mill 9. Needle grinding 10. 11. Germ hydrocyclone germ sieve 12. Pressure curved sieve 13. 14. Sink separator Corn starch process introduction: 1. corn starch process Raw material requirements: Corn: moisture % (m/m) ≤ 14%; The impurity rate % ≤2% ; Starch content % (m/m) ≥70% ; Starch: 65-68% Germ of 6-8% Fiber powder 8-10% Protein powder4.5 - 6% A ton of corn can produce about 0.3-0.32 tons alcohol traditional corn wet milling method (sulfurous acid aqueous solution reflux soak corn to extract the constituent of soluble corn soaking water, tooth grinding crushing, hydrocyclone separation and extraction of corn germ, screening to slag, the disc separator and cyclone separator combined separation to remove protein) closed cycle production of corn starch production process, to guarantee the reliability of the process. At the same time make full use of process water, achieve the goal of saving water. Corn starch use corn as the raw material, through raw grain cleaning, soaking, crushing, fine grinding and separation, starch delicate, dehydration, drying, packaging, finished product. Production...
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...Book 3 - Technology, use and potentialities of Latin American starchy tubers Chapter 18 Starch-based plastics Olivier Vilpoux1 & Luc Averous.2 18.1. Introduction Brazil disposes of approximately 240,000 tons of waste per day, an amount lower than that in the USA (607,000 t./day), but fairly above those in countries such as Germany (85,000 t./day) and Sweden (10,400 t./day). Of that total, a large amount goes to open waste deposits. Only a small quantity is disposed of in appropriate places. A city like São Paulo spends, per day, US$ 300,000 with waste. According to the Instituto de Pesquisas Tecnológicas – IPT (Institute of Technological Research), only few municipalities have specific teams and public policies engaged in dealing with the waste. When it is not treated, waste becomes a serious sanitary problem, since it exposes the community to illnesses like diarrhea, amebiasis, and parasitosis, apart from contaminating the soil, waters and water tables. Among solutions, there are the creation of sanitary landfills in appropriate places, adoption of selective collecting and recycling programs, conducting of campaigns with the aim of both making society conscious about the problem and demanding a greater participation of government authorities (Editora Abril, 2002). The manufacturing of biodegradable material offers an interesting solution for plastic materials. Like it happens with organic residues, such as foodstuff, elimination of biodegradable materials ...
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...that increase the rate of reactions without changing chemical equilibrium between reactants and products (Cooper, 2000). These enzymes have a distinct chemical composition that constructs an active site for substrates to bind to; this is the location where the substances come together to from an enzyme-substrate complex, which makes forming a product possible. The shape of the molecule is extremely important to its function. Enzymes are composed of unique three-dimensional conformations, due to the complex folding during the secondary, tertiary, and quaternary, stages of protein production. Extreme pH levels, heat, concentration, and other factors can easily denature these exclusive structures. α-amylase is a biological catalyst found in the saliva of various organisms, including humans. It functions as a catalyst for the hydrolysis of starch products located in consumed foods. Chemically, starch is comprised of two different molecules, amylose and amylopectin. The glucose molecules in amylose are connected in a liner/straight manner, whereas, the glucose in amylopectin are arranged in a spiral shape. These unique linkages are what give this molecule its overall shape, and ultimately, its function. Starches produced in plants are normally a combination of both these molecules at a 30:70 ratio favoring amylopectin. The standard experiment done to detect if a substance contains starch is a reaction with Iodine (I2KI). The I2 in the reaction will react with the amylose molecules...
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...Amylases are enzymes capable of hydrolysing starch and related saccharides. The best known are alpha-amylase, beta-amylase and glucoamylase. Starch degrading enzymes like amylase has received attention of their biotechnological significance and economic benefits. Alpha amylases are one of the important and widely used enzymes whose spectrum of applications has widened in many sectors such as clinical, medicinal and analytical chemistry.The fungal isolates include Aspergillus species, Mucor species and Rhizopus species. The parameters such as optimal incubation period, pH and starch concentrations were considered in the study. Overall result showed maximum activity at acidic Ph 6 at concentration of 3% and 5% with 4 days of incubation...
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...extract maximum returns out of an Investment, one needs to keep eyes and ears wide open to sense early any opportunity coming in the way. In the last report titled ' Comprehensive Analysis of Indian Starch & Starch Derivatives Sector ' a deep tabular and a brief theoretical analysis was presented of last 10 Years' Data to understand the scope and potential of Indian Starch & Starch Derivatives Sector. Now, in this report, we will present a deep theoretical argument based on the study of Chinese Starch & Starch Derivatives Sector over last two decades to understand the tremendous growth opportunity Indian Starch & Starch Derivatives Sector provides in the coming decade. It is worthwhile to note here that Indian Sector is almost a replica of Chinese Sector and Indian Sector, as at 2010, is at a stage where Chinese Sector was there in 1992. Over the time period between 1992-2009, Chinese Starch & Starch Derivatives Sector has grown more than 10 times and Indian Starch & Starch Derivatives Sector is bound to replicate such growth in the span of coming 10 years. We will also include in our analysis the consumption-trend of Sugar vis-a-vis Starch in China as well as India as Starch-based sweetners form 50 % of the marketsize of Chinese Starch & Starch Derivatives Sector whereas use of Starch-based sweetners as a substitute to sugar is only now catching up fast in India. We will also discuss briefly in this report the valuation aspect of Chinese and Indian sector at macro level and micro...
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...(C) Iodine Test for Starch Iodine test was used to test for the presence of starch in the given food material. When iodine solution was mixed with starch it turned from brown to dark blue in color. The reaction could take place even with little amount of starch as iodine test was a sensitive one. Iodine solution penetrated easily into the starch when the cell walls have been destroyed, hence chopping and crunching or biscuit-cream cracker, potato and groundnut were needed. IN the experiment conducted, biscuit-cream cracker, potato and groundnut showed positive results against the test, indicating starch was present in these food samples. However different color intensity was shown. Starch was a polysaccharide consisting of glucose units joined together by glycosidic bonds. Starch existed in two types of molecules which were amylose and amylopectin. Generally starch contained more amylopectin (65% to 85%) compare to amylose. The reaction between amylose and iodine was said to account the color change of the solution as the present of amylose was higher in most of the starch product. Amylose molecules consisted of single, mostly unbranched chains of glucose molecules and had helical structure which was a coil or spring. Iodine on its own was insoluble in water, therefore potassium triiodide solution was used as a reagent, as it dissociated, the iodide ion reacted reversibly with the iodine to yield the triiodine ion. A further reaction between a triidide ion and an iodine molecule...
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...addition, this range is the most acceptance level by the consumer. 6. Nutritional value Chickpea pasta also have high nutritional value in mineral and fat where 25% chickpea flour has a significantly lower glycaemic index and a higher mineral content than durum wheat pasta. Legumes are characterized by high contents of minerals, which depend on the species and cultivar and also on the characteristics of the soil where the plants are grown. The presence of non digestible constituents of chickpea flour is one of the factor making digestible starch slowly digest and low in starch hydrolysis. It includes resistant starch, oligosaccharide, polyphenols and lectins. The total starch level in chickpea indicates a dilution effect by the lesser starch content (Esmat, 2010). The final product of chickpea...
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...content of those foods. In order to determine which specific major molecules were present in certain foods we conducted four different tests. The iodine test tested for starch by putting a few drops of iodine on a food substance. If the food turned a blue-black color then it was positive for starch. The Benedict’s test tested for the presence of sugar. In order to test you put 1 ml of solution to be tested and add an equal volume of Benedict’s reagent in a test tube and heat in a water bath for two minutes. If the solution goes from blue, to green-yellow, then to a red-brown color it means it is positive for sugar. The next test is the Biuret test and its tests for peptide bonds in proteins. To test you add a few drops of Biuret reagent to a food substance. If it turns a violet-purple color then it is positive for protein. The final test is the Grease Spot test for lipids. If there is a “grease spot” present after conducting this test, then the food tested positive for lipids. The first group of foods that were tested was the fruits and they consisted of apples, bananas, plantains, and avocados. The apple, banana, and plantain contained starch and sugar and this is correct because these two macromolecules are present in plants. The only exception to this group is the avocado. It contains a small amount of starch (since it is still a plant), but it also contains proteins and lipids because of its single large pit (seed) whereas the other fruits listed above have numerous smaller...
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...Project Report Writing Project title: To show that starch is formed as a result of photosynthesis. Introduction This report writing is about conducting an experiment to show that starch is formed as a result of photosynthesis (testing for starch in a green leaf). It would talk about the aim, list of all apparatus needed and the different methods used. It would also illustrate the three conditions necessary for photosynthesis that is, if carbon dioxide, sunlight and chlorophyll are necessary for photosynthesis. In addition, a test would be carried out to demonstrate that starch is formed as a result of photosynthesis. Last but not the least, outcomes expected from each test carried out would be given in this report after each investigation is done. Plan Plan is to research on relevant information for my project and to produce a written report at the end. I would be researching about the apparatus and equipments that are required for the experiment. Would ensure good lab practice is followed at all times when a test is being carried out. I would research on methods or procedures that are needed to carry out the experiment. Again would be carrying out practical work in each session to improve the design of the project. I would also research on conditions necessary for photosynthesis, which is to investigate the need for carbon dioxide in photosynthesis. Last but not the least; investigate if the necessity of sunlight and chlorophyll are necessary for photosynthesis. Aim ...
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...Functions of Potassium in Plants P When water supply is short, K is pumped out of the guard cells. The pores close tightly to prevent loss of water and minimize drought stress to the plant. If K supply is inadequate, the stomates become sluggish – slow to respond – and water vapor is lost. Closure may take hours rather than Potassium (K) increases Enzyme Activation minutes and is incomplete. crop yield and improves Enzymes serve as cataAs a result, plants with an quality. It is required for lysts for chemical reactions, insufficient supply of K are numerous plant growth being utilized but not conmuch more susceptible to processes. sumed in the process. They water stress. bring together other molecules in such a way Accumulation of K in plant roots prothat the chemical reaction can take place. duces a gradient of osmotic pressure that Potassium “activates” at least 60 different draws water into the roots. Plants deficient in enzymes involved in plant growth. The K K are thus less able to absorb water and are changes the physical shape of the enzyme more subject to stress when water is in short molecule, exposing the appropriate chemical- supply. ly active sites for reaction. Potassium also neutralizes various organic anions and other Photosynthesis compounds within the plant, helping to stabiThe role of K in photosynthesis is comlize pH between 7 and 8...optimum for most plex. The activation of enzymes by K and its enzyme reactions. involvement...
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... solutions. Results: Table 1: Observations made when two carbohydrate solutions provided in laboratory were tested with Benedict and Iodine solution. | Observations | Conclusions | Solution A | Benedict’s test: An initial blue translucent mixture turned to brick-red opaque solution and moderate amount of precipitate settled after heated at a high temperature for two minute. | Presence of reducing sugar | | Iodine test: The translucent colouration of the mixture retained its yellowish-brown colour. | Absence of starch | Solution B | Benedict’s test: The translucent colouration of the mixture remained its blue colour. | Absence of reducing sugar | | Iodine test: The initial yellowish-brown translucent mixture turned to bluish-black opaque solution when solution was mixed. | Presence of starch | Table 2: Colour reactions of Benedict’s test for saliva and 3M hydrochloric acid in two carbohydrate solutions provided in laboratory. Tube | Contents | Temperature (ºC) | Benedict’s Test—Colour...
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...Practical 2 Cell Biology Biological Studies II molecules II Practical 3 Cell Biology Studies III Practical 4 Cell Biology Studies IV Practical 8 Cell Biology Studies VIII Practical 5 Cell Biology Studies V Practical 6 Cell Biology Studies VI Practical 7 Cell Biology Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages of Plant Mitosis and Meiosis DNA, Mitosis and Meiosis Modelling 71 Respiration of Yeast 93 Practical 3 Enzyme studies I (Experiment 1) Optional: Practical 3 Enzyme...
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...Macromolecules Carbohydrates are a type of macromolecule. Their subunit or monomer are simple sugars such as glucose and fructose. These are created with hydrogen bonds and are inorganic CH2OH. Glucose an example of a carbohydrates function is to provide energy for the organs to function in the body. Carbohydrates are a from of short term energy. A polysaccharide starch consists of repeating glucose molecules and has the function of breaking back down into glucose to again provide energy to the body. Macromolecules break down by hydrolysis a process of removing water to create a polymer. Lipids or fats are another form of macromolecules. The structure of a lipid consists of carbo to hydrogen bonds and carbon to oxygen single and double bond,...
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