For this examination, we quantified the speed of the compound response of hydrogen peroxide and guaiacol with the chemical peroxidase, removed from turnips. We tried this response first, by using three pH levels; pH 4.0, pH 7.0 and pH 8.0 to see which pH was ideal for the response of hydrogen peroxide and guaiacol. We utilized a colorimeter to quantify the measure of light that went through the substance at various times amid the response. Based off of our perusing of the colorimeter, we ascertained the underlying response speed of the concoction response and confirmed that pH 7.0 was the ideal pH for this compound response since it had the most elevated IRV. INTRODUCTION:
Enzymes are very unique and important to the well being of humans, …show more content…
We needed a substrate(guaiacol and hydrogen peroxide), enzyme(1ml of turnip peroxidase), deionized water, .3 ml of guaiacol, and .3 ml of hydrogen peroxide. Then fill a cuvette with more than half of deionized water . Next, we added .3 ml of guaiacol and 1ml of turnip peroxidase to the deionized water. After that, we covered it with Parafilm and then began to mix. The next step to the experiment is to observe. After every five minutes, we would observe the color of the reaction , and whether it changed. RESULTS:
The information we gathered demonstrates our speculation since it demonstrates that the quickest enzymatic response rate happens when the turnip peroxidase focus is 2%. Our information demonstrates the test tube with 2% peroxidase achieving 100% oxygen in just two minutes instead of more than 5 for both .5% and 1%. Our resulting unmistakably demonstrate that the response is significantly quicker with a higher compound concentration causing it to turn brownish. DISCUSSION:
As the group discussed the experiment, we were not surprised by the results. On the other hand ,we wondered that if we lowered the amount of enzyme solution would the reaction take longer, speed up, or remain constant . We also can see if the reaction changes to the same color as this